LOCUS       P527                    4382 bp ds-DNA     circular     21-MAY-2020
DEFINITION  .
ACCESSION   urn.local...md-5xoajnh                                             
VERSION     urn.local...md-5xoajnh                                             
COMMENT     
COMMENT     Module C
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    577..597
                     /locus_tag="oCS2174"
                     /ApEinfo_label="oCS2174"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      join(3528..4382,1..2)
                     /locus_tag="High Copy Ori"
                     /ApEinfo_label="High Copy Ori"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Terminator      2143..2358
                     /locus_tag="g7 terminator"
                     /ApEinfo_label="g7 terminator"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    537..2138
                     /locus_tag="RcTAL"
                     /ApEinfo_label="RcTAL"
                     /ApEinfo_fwdcolor="#ff66ff"
                     /ApEinfo_revcolor="#ff66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(818..839)
                     /locus_tag="oCS2172"
                     /ApEinfo_label="oCS2172"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            2407..3460
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Promoter        24..449
                     /locus_tag="CaMV 35S promoter short"
                     /ApEinfo_label="CaMV 35S promoter short"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2097..2115)
                     /locus_tag="oCS2173"
                     /ApEinfo_label="oCS2173"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    536..536
                     /locus_tag="BsaI overhang"
                     /ApEinfo_label="BsaI overhang"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1758..1776
                     /locus_tag="oCS2175"
                     /ApEinfo_label="oCS2175"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2139..2142
                     /locus_tag="BsaI overhang(1)"
                     /ApEinfo_label="BsaI overhang"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1050..1068
                     /locus_tag="oCS2176"
                     /ApEinfo_label="oCS2176"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     5'_UTR          466..527
                     /locus_tag="TMV Omega"
                     /ApEinfo_label="TMV Omega"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3255..3260
                     /locus_tag="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2383..2389
                     /locus_tag="AarI site (N4v top strand; N8v bottom strand)"
                     /ApEinfo_label="AarI site (N4v top strand; N8v bottom
                     strand)"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1..7)
                     /locus_tag="AarI site (N4v top strand; N8v bottom
                     strand)(1)"
                     /ApEinfo_label="AarI site (N4v top strand; N8v bottom
                     strand)"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#66ffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4373..4379)
                     /locus_tag="SapI site"
                     /ApEinfo_label="SapI site"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    12..15
                     /locus_tag="AarI overhang"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2375..2378
                     /locus_tag="AarI overhang(1)"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cacctgctca tCCGGggcgc gccGTCAACA TGGTGGAGCA CGACACTCTG GTCTACTCCA
       61 AAAATGTCAA AGATACAGTC TCAGAAGATC AAAGGGCTAT TGAGACTTTT CAACAAAGGA
      121 TAATTTCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCGAAAGGA
      181 CAGTAGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCA
      241 TTCAAGATCT CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG
      301 TGGAAAAAGA AGAGGTTCCA ACCACGTCTA CAAAGCAAGT GGATTGATGT GACATCTCCA
      361 CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG
      421 GAAGTTCATT TCATTTGGAG AGGACACGCT CGAGTATAAG AGCTCATTTT TACAACAATT
      481 ACCAACAACA ACAAACAACA AACAACATTA CAATTACATT TACAATTATC GATACAATGA
      541 CGCTCCAATC ACAGACTGCT AAAGACTGCC TTGCATTAGA TGGCGCTTTA ACGCTGGTGC
      601 AATGCGAGGC CATCGCTACA CATAGGTCCC GAATAAGCGT CACtCCTGCC CTCAGAGAGA
      661 GATGTGCAAG GGCTCACGCC CGTCTGGAAC ACGCCATCGC CGAGCAAAGA CATATATACG
      721 GCATAACCAC TGGGTTTGGC CCGCTTGCAA ATCGACTGAT TGGAGCAGAT CAAGGCGCAG
      781 AACTCCAACA AAACCTTATT TACCACCTTG CCACGGGGGT TGGGCCAAAA TTGAGTTGGG
      841 CTGAGGCAAG GGCCCTGATG TTGGCCAGGC TGAACTCAAT TCTCCAGGGA GCTTCAGGGG
      901 CTTCCCCAGA GACAATCGAT CGTATCGTAG CAGTACTCAA CGCTGGATTT GCCCCCGAGG
      961 TTCCGGCTCA AGGAACTGTC GGCGCAAGCG GTGACCTGAC CCCGCTCGCA CACATGGTGC
     1021 TCGCTCTGCA GGGCCGAGGT CGTATGATTG ACCCAAGTGG CCGTGTTCAA GAGGCCGGAG
     1081 CCGTCATGGA TCGTCTGTGC GGTGGGCCCC TGACCCTGGC TGCCAGgGAC GGACTGGCAC
     1141 TGGTAAACGG AACTTCCGCT ATGACGGCCA TTGCCGCCCT CACCGGTGTA GAAGCTGCCA
     1201 GAGCCATAGA TGCTGCTTTA AGGCACTCCG CTGTACTTAT GGAAGTACTT AGCGGACACG
     1261 CAGAGGCATG GCATCCTGCC TTTGCAGAGC TTCGTCCTCA TCCAGGTCAA TTGAGAGCCA
     1321 CAGAGCGTCT TGCACAAGCC TTAGATGGCG CCGGTAGAGT GTGTAGGACC TTAACTGCTG
     1381 CAAGACGTCT tACCGCCGCC GACCTGAGGC CCGAGGACCA TCCCGCACAG GATGCCTACT
     1441 CATTGAGAGT AGTTCCGCAA TTAGTAGGGG CAGTCTGGGA CACACTTGAT TGGCATGACA
     1501 GGGTAGTCAC GTGCGAGTTA AACAGCGTCA CAGATAACCC TATATTTCCT GAGGGCTGCG
     1561 CTGTGCCTGC TCTCCATGGC GGGAACTTTA TGGGTGTACA TGTCGCACTC GCTTCTGATG
     1621 CACTGAACGC TGCATTGGTA ACTCTGGCTG GTTTAGTTGA GAGGCAGATC GCAAGACTGA
     1681 CAGACGAGAA GTTGAATAAG GGCTTACCAG CATTCCTCCA CGGAGGACAG GCTGGATTAC
     1741 AAAGTGGGTT CATGGGGGCC CAAGTCACCG CTACTGCTTT GCTCGCCGAA ATGCGAGCTA
     1801 ACGCTACCCC tGTCTCAGTA CAGTCTCTTT CAACAAATGG CGCAAACCAG GACGTTGTGT
     1861 CCATGGGTAC GATTGCAGCC AGGCGAGCAC GAGCACAATT GTTACCGCTG TCACAGATTC
     1921 AGGCTATTTT GGCATTAGCT TTGGCACAAG CTATGGATTT GTTAGACGAT CCGGAAGGAC
     1981 AAGCAGGCTG GTCACTCACT GCCAGgGACC TCAGGGACCG AATCAGGGCT GTAAGTCCCG
     2041 GATTACGTGC CGATAGGCCA CTTGCCGGGC ACATTGAGGC AGTCGCACAA GGACTTCGTC
     2101 ATCCATCTGC CGCTGCCGAC CCTCCGGCTA GTGGTTGAGC TTGATCCCCC GTCGACAGCT
     2161 AGCTATATCA TCAATTTATG TATTACACAT AATATCGCAC TCAGTCTTTC ATCTACGGCA
     2221 ATGTACCAGC TGATATAATC AGTTATTGAA ATATTTCTGA ATTTAAACTT GCATCAATAA
     2281 ATTTATGTTT TTGCTTGGAC TATAATACCT GACTTGTTAT TTTATCAATA AATATTTAAA
     2341 CTATATTTCT TTCAAGATcc agagctctgg tgacAGTGgt gggcaggtgt gatgaaaagc
     2401 cttagggtca ggtggcactt ttcggggaaa tgtgcgatga acccctattt gtttattttt
     2461 ctaaatacat tcaaatatgt atccgctaat gagacaataa ccctgataaa tgcttcaatt
     2521 atattgaaaa aggaagagta tgagtatcca acatttccgt gtcgccctta ttcccttttt
     2581 tgcggccttc tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2641 tgaagatcag ttgggtgccc gagtgggtta cattgaactg gatctcaaca gcggcaaaat
     2701 cttagagagt tttcgccccg aagaacgctt cccaatgatg agcactttca aagttctgct
     2761 atgtggcgcg gtgttatccc gtattgatgc tgggcaagag caactcggtc gccgcataca
     2821 ctattctcag aatgacttgg ttgagtattc accagtcaca gaaaagcatc ttacggatgg
     2881 catgacagta agagaattat gtagtgctgc cataaccatg agtgataaca ctgcggccaa
     2941 cttacttctg acaacgattg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3001 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3061 cgagcgtgac accacgatgc ctgtagcaat ggcaacaact ctccgcaaac tattaactgg
     3121 cgaactactt actcttgctt cacgccagca actcattgac tggatggagg cggacaaagt
     3181 tgcaggacca cttctgcgct cggcacttcc ggctggctgg tttattgctg ataaatctgg
     3241 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3301 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3361 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacg aagggaaata
     3421 aatagtagcc cgcctgatgt gcgggctttt ttttggtggt ggttaccagt gtcgcttagc
     3481 taccgtaggt cctcttaggc ggtccgatta gcgacatagt catcaatgcc ttgacggcaa
     3541 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3601 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3661 gctaccagcg gtggtttgtt tgccggatca agagctacta actctttttc cgaaggtaac
     3721 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3781 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3841 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc tcacagccca gcttggagcg
     3961 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4081 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4141 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4201 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4261 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4321 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4381 cc     
//
