LOCUS       Exported                7595 bp ds-DNA     circular SYN 31-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Paola Rossolillo
  TITLE     Direct Submission
  JOURNAL   Exported Jan 31, 2020 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_recomb     1..34
                     /label=LoxP
     polyA_signal    224..272
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     239..260
                     /label=HSV TK p(A)-RV
     CDS             complement(367..1077)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     377..386
                     /label=mCherry RP
     misc_feature    complement(1114..1235)
                     /label=p10 promoter
     primer_bind     complement(1197..1216)
                     /label=p10-FW
     promoter        1275..1366
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1299..1318
                     /label=bac1
     misc_feature    1404..1479
                     /label=MSC 1
     primer_bind     complement(1519..1538)
                     /label=SV40polyA
     polyA_signal    1625..1759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     1878..3235
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1894..3235)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      3382..3837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4119..4223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4224..5084
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5255..5843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     6406..7233
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6406..6530
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(6568..7173)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctggtt taaacgtacc cgtagtggct
       61 atggcagggc ttgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
      121 ggttggggtg gggaaaagga agaaacgcgg gcgtattggt cccaatgggg tctcggtggg
      181 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
      241 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
      301 ctccttccgt gtttcagtta gcctccccca tctcccggta ccgcatgcta tgcatcagct
      361 gctagcttac ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc
      421 gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac
      481 gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc
      541 gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc
      601 gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac
      661 ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc
      721 ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg
      781 ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg
      841 cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg
      901 cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc
      961 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
     1021 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatacc
     1081 tcctggctcg agatcccggg tgatcaagtc ttcgtcgagt gattgtaaat aaaatgtaat
     1141 ttacagtata gtattttaat taatatacaa atgatttgat aataattctt atttaactat
     1201 aatatattgt gttgggttga attaaaggtc cgtatactag tatcgattcg cgacctactc
     1261 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     1321 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     1381 ataccgtccc accatcgggc gcggatcccg gtccgaagcg cgcggaattc aaaggcctac
     1441 gtcgacgagc tcactagtcg cggccgcttt cgaatctaga gcctgcagtc tcgacaagct
     1501 tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc
     1561 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     1621 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1681 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1741 atcttatcat gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag
     1801 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactatca taacccctag
     1861 gcgattaatt gatccgggtt attagtacat ttattaagcg ctagattctg tgcgttgttg
     1921 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
     1981 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
     2041 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
     2101 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
     2161 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
     2221 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
     2281 actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt
     2341 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
     2401 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
     2461 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
     2521 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
     2581 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
     2641 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
     2701 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
     2761 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
     2821 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
     2881 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
     2941 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
     3001 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
     3061 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
     3121 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
     3181 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat
     3241 cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt
     3301 cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3361 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     3601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3721 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     3841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3901 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3961 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4021 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4081 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5401 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5881 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5941 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6001 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6061 ggccgattca ttaatgcaga acggctccgc ccactattaa tgaaattaaa aattccaatt
     6121 ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga aagaaaaatg
     6181 tcgtcgacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa atattgaacg
     6241 atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt atactaaact
     6301 gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta atcaaggctc
     6361 tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat cttttaatca
     6421 aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtc gacaagctct
     6481 gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga ataataaaac
     6541 aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa caagaacatt
     6601 tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat atttaaaatc
     6661 attttcaaat gattcacagt taatttgcga caatataatt ttattttcac ataaactaga
     6721 cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct cataaaaatt
     6781 aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt ttttgttgtc
     6841 ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag tttttctgta
     6901 atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa ttattaaatt
     6961 tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg catagtacgc
     7021 agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat aactttccaa
     7081 aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac tattgtctgc
     7141 gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa actaatatca
     7201 caaactggaa atgtctatca atatatagtt gctgatatcc cattttacca ccataatact
     7261 taaaatgatc cacaaacaag ccaccaccaa tcgctacact caccgccgct acagacgcta
     7321 caatcacctc atatttattc tcctcacata tttattctat tctaattctc aatcatacca
     7381 atcaacttct catactcaat cataccaatc aatacacatt atactactac tcatttccaa
     7441 tcattcacat tcataatata tctgttatac tactccttat cactactctc tccatccaca
     7501 ttattatcct ccatcatacg acgctacata actacgtggt tcctaactac accaagcata
     7561 gccctagcca gccagccaca ccttacagcc ttttt
//
