LOCUS       Exported                6564 bp ds-DNA     circular SYN 31-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Paola Rossolillo
  TITLE     Direct Submission
  JOURNAL   Exported Jan 31, 2020 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    224..272
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     239..260
                     /label=HSV TK p(A)-RV
     misc_feature    362..378
                     /label=MSC 2
     misc_feature    complement(401..522)
                     /label=p10 promoter
     primer_bind     complement(484..503)
                     /label=p10-FW
     promoter        562..653
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     586..605
                     /label=bac1
     CDS             704..727
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    729..804
                     /label=MSC 1
     primer_bind     complement(844..863)
                     /label=SV40polyA
     polyA_signal    950..1084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     1203..2560
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1219..2560)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      complement(2712..3152)
                     /direction=LEFT
                     /label=F1 ori
     promoter        3444..3548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3549..4409
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4580..5168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     5731..6558
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5731..5855
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5893..6498)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctggtt taaacgtacc cgtagtggct
       61 atggcagggc ttgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
      121 ggttggggtg gggaaaagga agaaacgcgg gcgtattggt cccaatgggg tctcggtggg
      181 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
      241 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
      301 ctccttccgt gtttcagtta gcctccccca tctcccggta ccgcatgcta tgcatcagct
      361 gctagcacca tggctcgaga tcccgggtga tcaagtcttc gtcgagtgat tgtaaataaa
      421 atgtaattta cagtatagta ttttaattaa tatacaaatg atttgataat aattcttatt
      481 taactataat atattgtgtt gggttgaatt aaaggtccgt atactagtat cgattcgcga
      541 cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata
      601 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg
      661 attattcata ccgtcccacc atcgggcgcg gatcgccacc atggactaca aggacgacga
      721 tgacaaggga tcccggtccg aagcgcgcgg aattcaaagg cctacgtcga cgagctcact
      781 agtcgcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg agaagtacta
      841 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      901 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1081 gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg
     1141 agttggctgc tgccaccgct gagcaataac tatcataacc cctaggcgat taattgatcc
     1201 gggttattag tacatttatt aagcgctaga ttctgtgcgt tgttgattta cagacaattg
     1261 ttgtacgtat tttaataatt cattaaattt ataatcttta gggtggtatg ttagagcgaa
     1321 aatcaaatga ttttcagcgt ctttatatct gaatttaaat attaaatcct caatagattt
     1381 gtaaaatagg tttcgattag tttcaaacaa gggttgtttt tccgaaccga tggctggact
     1441 atctaatgga ttttcgctca acgccacaaa acttgccaaa tcttgtagca gcaatctagc
     1501 tttgtcgata ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt
     1561 atgcgctttt gtatttcttt catcactgtc gttagtgtac aattgactcg acgtaaacac
     1621 gttaaataaa gcttggacat atttaacatc gggcgtgtta gctttattag gccgattatc
     1681 gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa gacgattttg ccatagccac
     1741 acgacgccta ttaattgtgt cggctaacac gtccgcgatc aaatttgtag ttgagctttt
     1801 tggaattatt tctgattgcg ggcgtttttg ggcgggtttc aatctaactg tgcccgattt
     1861 taattcagac aacacgttag aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa
     1921 atctactaat ggcggcggtg gtggagctga tgataaatct accatcggtg gaggcgcagg
     1981 cggggctggc ggcggaggcg gaggcggagg tggtggcggt gatgcagacg gcggtttagg
     2041 ctcaaatgtc tctttaggca acacagtcgg cacctcaact attgtactgg tttcgggcgc
     2101 cgtttttggt ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa tagcttccaa
     2161 caattgttgt ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca ttggtggagc
     2221 gggcggcaat tcagacatcg atggtggtgg tggtggtgga ggcgctggaa tgttaggcac
     2281 gggagaaggt ggtggcggcg gtgccgccgg tataatttgt tctggtttag tttgttcgcg
     2341 cacgattgtg ggcaccggcg caggcgccgc tggctgcaca acggaaggtc gtctgcttcg
     2401 aggcagcgct tggggtggtg gcaattcaat attataattg gaatacaaat cgtaaaaatc
     2461 tgctataagc attgtaattt cgctatcgtt taccgtgccg atatttaaca accgctcaat
     2521 gtaagcaatt gtattgtaaa gagattgtct caagctcgga tcgatcccgc acgccgataa
     2581 caagcctttt catttttact acagcattgt agtggcgaga cacttcgctg tcgtcgcctg
     2641 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     2701 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     2761 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2821 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2881 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     2941 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3001 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     3061 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3121 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     3181 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     3241 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3301 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     3361 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5281 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5341 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5401 gcagaacggc tccgcccact attaatgaaa ttaaaaattc caattttaaa aaacgcagca
     5461 agagaaacat ttgtatgaaa gaatgcgtag aaggaaagaa aaatgtcgtc gacatgctga
     5521 acaacaagat taatatgcct ccgtgtataa aaaaaatatt gaacgatttg aaagaaaaca
     5581 atgtaccgcg cggcggtatg tacaggaaga ggtttatact aaactgttac attgcaaacg
     5641 tggtttcgtg tgccaagtgt gaaaaccgat gtttaatcaa ggctctgacg catttctaca
     5701 accacgactc caagtgtgtg ggtgaagtca tgcatctttt aatcaaatcc caagatgtgt
     5761 ataaaccacc aaactgccaa aaaatgaaaa ctgtcgacaa gctctgtccg tttgctggca
     5821 actgcaaggg tctcaatcct atttgtaatt attgaataat aaaacaatta taaatgctaa
     5881 atttgttttt tattaacgat acaaaccaaa cgcaacaaga acatttgtag tattatctat
     5941 aattgaaaac gcgtagttat aatcgctgag gtaatattta aaatcatttt caaatgattc
     6001 acagttaatt tgcgacaata taattttatt ttcacataaa ctagacgcct tgtcgtcttc
     6061 ttcttcgtat tccttctctt tttcattttt ctcctcataa aaattaacat agttattatc
     6121 gtatccatat atgtatctat cgtatagagt aaattttttg ttgtcataaa tatatatgtc
     6181 ttttttaatg gggtgtatag taccgctgcg catagttttt ctgtaattta caacagtgct
     6241 attttctggt agttcttcgg agtgtgttgc tttaattatt aaatttatat aatcaatgaa
     6301 tttgggatcg tcggttttgt acaatatgtt gccggcatag tacgcagctt cttctagttc
     6361 aattacacca ttttttagca gcaccggatt aacataactt tccaaaatgt tgtacgaacc
     6421 gttaaacaaa aacagttcac ctcccttttc tatactattg tctgcgagca gttgtttgtt
     6481 gttaaaaata acagccattg taatgagacg cacaaactaa tatcacaaac tggaaatgtc
     6541 tatcaatata tagttgctga tatc
//
