LOCUS       Exported                6913 bp ds-DNA     circular SYN 31-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 31, 2020 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     1..34
                     /label=LoxP
     polyA_signal    224..272
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     239..260
                     /label=HSV TK p(A)-RV
     misc_feature    362..378
                     /label=MSC 2
     misc_feature    complement(401..522)
                     /label=p10 promoter
     primer_bind     complement(484..503)
                     /label=p10-FW
     promoter        562..653
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     586..605
                     /label=bac1
     misc_feature    691..766
                     /label=MSC 1
     CDS             768..797
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(837..856)
                     /label=SV40polyA
     polyA_signal    943..1077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     1196..2553
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1212..2553)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      2700..3155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3437..3541
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3542..4402
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4573..5161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     5724..6551
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5724..5848
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5886..6491)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctggtt taaacgtacc cgtagtggct
       61 atggcagggc ttgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
      121 ggttggggtg gggaaaagga agaaacgcgg gcgtattggt cccaatgggg tctcggtggg
      181 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
      241 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
      301 ctccttccgt gtttcagtta gcctccccca tctcccggta ccgcatgcta tgcatcagct
      361 gctagcacca tggctcgaga tcccgggtga tcaagtcttc gtcgagtgat tgtaaataaa
      421 atgtaattta cagtatagta ttttaattaa tatacaaatg atttgataat aattcttatt
      481 taactataat atattgtgtt gggttgaatt aaaggtccgt atactagtat cgattcgcga
      541 cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata
      601 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg
      661 attattcata ccgtcccacc atcgggcgcg gatcccggtc cgaagcgcgc ggaattcaaa
      721 ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga atctagacac catcatcacc
      781 atcaccatca ccaccattaa ctgcagtctc gacaagcttg tcgagaagta ctagaggatc
      841 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      901 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      961 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     1021 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga
     1081 tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     1141 tgctgccacc gctgagcaat aactatcata acccctaggc gattaattga tccgggttat
     1201 tagtacattt attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg
     1261 tattttaata attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa
     1321 tgattttcag cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat
     1381 aggtttcgat tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat
     1441 ggattttcgc tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg
     1501 atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct
     1561 tttgtatttc tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat
     1621 aaagcttgga catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg
     1681 tcccaaccct cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc
     1741 ctattaattg tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt
     1801 atttctgatt gcgggcgttt ttgggcgggt ttcaatctaa ctgtgcccga ttttaattca
     1861 gacaacacgt tagaaagcga tggtgcaggc ggtggtaaca tttcagacgg caaatctact
     1921 aatggcggcg gtggtggagc tgatgataaa tctaccatcg gtggaggcgc aggcggggct
     1981 ggcggcggag gcggaggcgg aggtggtggc ggtgatgcag acggcggttt aggctcaaat
     2041 gtctctttag gcaacacagt cggcacctca actattgtac tggtttcggg cgccgttttt
     2101 ggtttgaccg gtctgagacg agtgcgattt ttttcgtttc taatagcttc caacaattgt
     2161 tgtctgtcgt ctaaaggtgc agcgggttga ggttccgtcg gcattggtgg agcgggcggc
     2221 aattcagaca tcgatggtgg tggtggtggt ggaggcgctg gaatgttagg cacgggagaa
     2281 ggtggtggcg gcggtgccgc cggtataatt tgttctggtt tagtttgttc gcgcacgatt
     2341 gtgggcaccg gcgcaggcgc cgctggctgc acaacggaag gtcgtctgct tcgaggcagc
     2401 gcttggggtg gtggcaattc aatattataa ttggaataca aatcgtaaaa atctgctata
     2461 agcattgtaa tttcgctatc gtttaccgtg ccgatattta acaaccgctc aatgtaagca
     2521 attgtattgt aaagagattg tctcaagctc ggatcgatcc cgcacgccga taacaagcct
     2581 tttcattttt actacagcat tgtagtggcg agacacttcg ctgtcgtcgc ctgatgcggt
     2641 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     2701 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2761 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2821 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2881 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     2941 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3001 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     3061 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3121 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     3181 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3241 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3301 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3361 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3421 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3481 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3541 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3601 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3661 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3721 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3781 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3841 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3901 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3961 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4021 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4081 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4141 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4201 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4261 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4321 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4381 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4441 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4501 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4681 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4981 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5221 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5281 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5341 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagaac
     5401 ggctccgccc actattaatg aaattaaaaa ttccaatttt aaaaaacgca gcaagagaaa
     5461 catttgtatg aaagaatgcg tagaaggaaa gaaaaatgtc gtcgacatgc tgaacaacaa
     5521 gattaatatg cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc
     5581 gcgcggcggt atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc
     5641 gtgtgccaag tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga
     5701 ctccaagtgt gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg tgtataaacc
     5761 accaaactgc caaaaaatga aaactgtcga caagctctgt ccgtttgctg gcaactgcaa
     5821 gggtctcaat cctatttgta attattgaat aataaaacaa ttataaatgc taaatttgtt
     5881 ttttattaac gatacaaacc aaacgcaaca agaacatttg tagtattatc tataattgaa
     5941 aacgcgtagt tataatcgct gaggtaatat ttaaaatcat tttcaaatga ttcacagtta
     6001 atttgcgaca atataatttt attttcacat aaactagacg ccttgtcgtc ttcttcttcg
     6061 tattccttct ctttttcatt tttctcctca taaaaattaa catagttatt atcgtatcca
     6121 tatatgtatc tatcgtatag agtaaatttt ttgttgtcat aaatatatat gtctttttta
     6181 atggggtgta tagtaccgct gcgcatagtt tttctgtaat ttacaacagt gctattttct
     6241 ggtagttctt cggagtgtgt tgctttaatt attaaattta tataatcaat gaatttggga
     6301 tcgtcggttt tgtacaatat gttgccggca tagtacgcag cttcttctag ttcaattaca
     6361 ccatttttta gcagcaccgg attaacataa ctttccaaaa tgttgtacga accgttaaac
     6421 aaaaacagtt cacctccctt ttctatacta ttgtctgcga gcagttgttt gttgttaaaa
     6481 ataacagcca ttgtaatgag acgcacaaac taatatcaca aactggaaat gtctatcaat
     6541 atatagttgc tgatatccca ttttaccacc ataatactta aaatgatcca caaacaagcc
     6601 accaccaatc gctacactca ccgccgctac agacgctaca atcacctcat atttattctc
     6661 ctcacatatt tattctattc taattctcaa tcataccaat caacttctca tactcaatca
     6721 taccaatcaa tacacattat actactactc atttccaatc attcacattc ataatatatc
     6781 tgttatacta ctccttatca ctactctctc catccacatt attatcctcc atcatacgac
     6841 gctacataac tacgtggttc ctaactacac caagcatagc cctagccagc cagccacacc
     6901 ttacagcctt ttt
//
