LOCUS       Exported                6901 bp ds-DNA     circular SYN 30-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2020 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    224..272
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     239..260
                     /label=HSV TK p(A)-RV
     primer_bind     complement(348..366)
                     /label=Backbone-F
     misc_feature    362..378
                     /label=MSC 2
     misc_feature    complement(401..522)
                     /label=p10 promoter
     primer_bind     complement(484..503)
                     /label=p10-FW
     promoter        562..653
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     576..595
                     /label=pBakPAC-FP
     primer_bind     586..605
                     /label=bac1
     misc_feature    691..766
                     /label=MSC 1
     CDS             768..785
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(825..844)
                     /label=SV40polyA
     polyA_signal    931..1065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     1184..2541
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1200..2541)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      2688..3143
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3425..3529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3530..4390
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4561..5149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     5712..6539
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5712..5836
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5874..6479)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctggtt taaacgtacc cgtagtggct
       61 atggcagggc ttgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
      121 ggttggggtg gggaaaagga agaaacgcgg gcgtattggt cccaatgggg tctcggtggg
      181 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
      241 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
      301 ctccttccgt gtttcagtta gcctccccca tctcccggta ccgcatgcta tgcatcagct
      361 gctagcacca tggctcgaga tcccgggtga tcaagtcttc gtcgagtgat tgtaaataaa
      421 atgtaattta cagtatagta ttttaattaa tatacaaatg atttgataat aattcttatt
      481 taactataat atattgtgtt gggttgaatt aaaggtccgt atactagtat cgattcgcga
      541 cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata
      601 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg
      661 attattcata ccgtcccacc atcgggcgcg gatcccggtc cgaagcgcgc ggaattcaaa
      721 ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga atctagacac catcaccatc
      781 accattaact gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat
      841 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      901 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
     1081 cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1141 tgagcaataa ctatcataac ccctaggcga ttaattgatc cgggttatta gtacatttat
     1201 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat
     1261 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg
     1321 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta
     1381 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc
     1441 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt
     1501 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt
     1561 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaataa agcttggaca
     1621 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg
     1681 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg
     1741 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc
     1801 gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta
     1861 gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt
     1921 ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc
     1981 ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc
     2041 aacacagtcg gcacctcaac tattgtactg gtttcgggcg ccgtttttgg tttgaccggt
     2101 ctgagacgag tgcgattttt ttcgtttcta atagcttcca acaattgttg tctgtcgtct
     2161 aaaggtgcag cgggttgagg ttccgtcggc attggtggag cgggcggcaa ttcagacatc
     2221 gatggtggtg gtggtggtgg aggcgctgga atgttaggca cgggagaagg tggtggcggc
     2281 ggtgccgccg gtataatttg ttctggttta gtttgttcgc gcacgattgt gggcaccggc
     2341 gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc ttggggtggt
     2401 ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat ctgctataag cattgtaatt
     2461 tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat tgtattgtaa
     2521 agagattgtc tcaagctcgg atcgatcccg cacgccgata acaagccttt tcatttttac
     2581 tacagcattg tagtggcgag acacttcgct gtcgtcgcct gatgcggtat tttctcctta
     2641 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     2701 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2761 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2821 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2881 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     2941 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3001 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     3061 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3121 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     3181 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3241 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3301 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3361 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3421 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3481 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3541 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3601 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3661 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3721 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3781 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3841 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3901 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3961 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4021 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4081 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4141 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4201 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4261 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4321 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4381 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4441 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4501 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4561 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4621 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4681 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4741 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4801 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4861 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4921 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4981 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5041 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5101 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5161 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5221 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5281 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5341 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagaacgg ctccgcccac
     5401 tattaatgaa attaaaaatt ccaattttaa aaaacgcagc aagagaaaca tttgtatgaa
     5461 agaatgcgta gaaggaaaga aaaatgtcgt cgacatgctg aacaacaaga ttaatatgcc
     5521 tccgtgtata aaaaaaatat tgaacgattt gaaagaaaac aatgtaccgc gcggcggtat
     5581 gtacaggaag aggtttatac taaactgtta cattgcaaac gtggtttcgt gtgccaagtg
     5641 tgaaaaccga tgtttaatca aggctctgac gcatttctac aaccacgact ccaagtgtgt
     5701 gggtgaagtc atgcatcttt taatcaaatc ccaagatgtg tataaaccac caaactgcca
     5761 aaaaatgaaa actgtcgaca agctctgtcc gtttgctggc aactgcaagg gtctcaatcc
     5821 tatttgtaat tattgaataa taaaacaatt ataaatgcta aatttgtttt ttattaacga
     5881 tacaaaccaa acgcaacaag aacatttgta gtattatcta taattgaaaa cgcgtagtta
     5941 taatcgctga ggtaatattt aaaatcattt tcaaatgatt cacagttaat ttgcgacaat
     6001 ataattttat tttcacataa actagacgcc ttgtcgtctt cttcttcgta ttccttctct
     6061 ttttcatttt tctcctcata aaaattaaca tagttattat cgtatccata tatgtatcta
     6121 tcgtatagag taaatttttt gttgtcataa atatatatgt cttttttaat ggggtgtata
     6181 gtaccgctgc gcatagtttt tctgtaattt acaacagtgc tattttctgg tagttcttcg
     6241 gagtgtgttg ctttaattat taaatttata taatcaatga atttgggatc gtcggttttg
     6301 tacaatatgt tgccggcata gtacgcagct tcttctagtt caattacacc attttttagc
     6361 agcaccggat taacataact ttccaaaatg ttgtacgaac cgttaaacaa aaacagttca
     6421 cctccctttt ctatactatt gtctgcgagc agttgtttgt tgttaaaaat aacagccatt
     6481 gtaatgagac gcacaaacta atatcacaaa ctggaaatgt ctatcaatat atagttgctg
     6541 atatcccatt ttaccaccat aatacttaaa atgatccaca aacaagccac caccaatcgc
     6601 tacactcacc gccgctacag acgctacaat cacctcatat ttattctcct cacatattta
     6661 ttctattcta attctcaatc ataccaatca acttctcata ctcaatcata ccaatcaata
     6721 cacattatac tactactcat ttccaatcat tcacattcat aatatatctg ttatactact
     6781 ccttatcact actctctcca tccacattat tatcctccat catacgacgc tacataacta
     6841 cgtggttcct aactacacca agcatagccc tagccagcca gccacacctt acagcctttt
     6901 t
//
