LOCUS       EF_tTpEF2               2201 bp ds-DNA     circular     06-SEP-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1609..1609
                     /label="Mut G->A, silent"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1489..1491)
                     /label="BspMI"
                     /ApEinfo_revcolor=#ff1827
                     /ApEinfo_fwdcolor=#ff1827
     misc_feature    complement(1493..2029)
                     /label="pro-CDS-Ter of SpecR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1057..1491)
                     /label="pro-CDS-Ter of SpecR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(2011..2013)
                     /label="BspMI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1507..1525
                     /label="SpecR R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1057..1491)
                     /label="Spec gene"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    248..287
                     /label="UNSX"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1493..1494)
                     /label="BspMI"
                     /ApEinfo_revcolor=#ff1827
                     /ApEinfo_fwdcolor=#ff1827
     misc_feature    complement(1057..1491)
                     /label="SpecR"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    complement(1493..1848)
                     /label="Spec gene"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(378..966)
                     /label="pMB1-pUC19 ORI variant"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1489..1491)
                     /label="BspMI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2015..2016)
                     /label="BspMI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    819..819
                     /label="pMB1 high-copy number mutation"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1493..1494)
                     /label="BspMI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1524..1543)
                     /label="SpecR F"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    288..317
                     /label="Extra region of pBR322"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1614..1619)
                     /label="BpmI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    43..82
                     /label="UNS1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1..42)
                     /label="In T-DNA region AH003392.2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     158             95..235
                     /label="Tp.EF2.term"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1883..1883
                     /label="Mut C->A"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(84..89)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1849..1996)
                     /label="specR_promoter_from_pKD13"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1493..1848)
                     /label="SpecR"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
ORIGIN
        1 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctCATTACTC GCATCCATTC
       61 TCAGGCTGTC TCGTCTCGTC TCaggtctcc gcttATATCT TCTTGCAACA ATGGTAGCGC
      121 GATTGATGAG ATAATAGATA AAAAAATCCA CTGTGAATGT TTTTCAATGA GTCTACAATA
      181 GTGACTTGAC AGGTCGTATA GAGTGAGACA TTAGTTATCT CTAACCAACC CTGCTcgctC
      241 GAgaccaCCA GGATACATAG ATTACCACAA CTCCGAGCCC TTCCACCcac agaatcaggg
      301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      541 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      781 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1021 cgttaaggga ttttggtcat gcattctagg tgattattat ttgccgacta ccttggtgat
     1081 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1141 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1201 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1261 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1321 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1381 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1441 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac cagcaagaat
     1501 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1561 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcac tctctccagg
     1621 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1681 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     1741 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     1801 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatgc gaaacgatcc
     1861 tcatcctgtc tcttgatcag atattgatcc cctgcgccat cagatccttg gcggcaagaa
     1921 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     1981 ttccggttcg cttgctaagc ttgcatgcct gcaagtcgac tctagaggag cggtgatcac
     2041 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     2101 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     2161 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg a
//