LOCUS       pEG103_mmPing20F       11402 bp    DNA     circular UNA 05-FEB-2020
DEFINITION  .
ACCESSION   urn.local...q-bizxbz5
VERSION     urn.local...q-bizxbz5
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /note="Geneious type: boarder"
                     /standard_name="Left Border"
     misc_feature    complement(373..924)
                     /note="Geneious type: motif"
                     /standard_name="BAR"
     promoter        1815..2651
     misc_feature    2516..2544
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="mPing_Flank_F(35S For)"
     misc_feature    2664..2684
                     /note="Geneious type: attB1"
                     /standard_name="attB1"
     misc_feature    2688..3333
                     /note="Geneious type: Transposon"
                     /standard_name="mmPing20F"
     promoter        2931..3140
     misc_feature    complement(3338..3358)
                     /note="Geneious type: attB2"
                     /standard_name="attB2"
     misc_feature    3367..4122
                     /note="Geneious type: reporter"
                     /standard_name="GFP-HIS6"
     primer_bind     complement(3447..3475)
                     /standard_name="mPing_Flank_R(GFP Rev)"
     terminator      4174..4900
     misc_feature    5125..5149
                     /note="Geneious type: boarder"
                     /standard_name="Right Border"
     misc_feature    complement(6210..7210)
                     /note="STA region from pVS1 plasmid"
                     /standard_name="STA region from pVS1 plasmid"
     source          <6210..>7210
                     /organism="Binary vector pCAMBIA-2300"
                     /mol_type="other DNA"
                     /db_xref="taxon:118407"
     rep_origin      complement(7803..8803)
     source          <7803..>8803
                     /organism="Binary vector pCAMBIA-2300"
                     /mol_type="other DNA"
                     /db_xref="taxon:118407"
     misc_feature    complement(9213..9473)
                     /note="bom site from pBR322"
                     /standard_name="bom site from pBR322"
     source          <9213..>9473
                     /organism="Binary vector pCAMBIA-2300"
                     /mol_type="other DNA"
                     /db_xref="taxon:118407"
     misc_feature    complement(9390..9409)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="VR"
     rep_origin      complement(9613..9893)
     source          <9613..>9893
                     /organism="Binary vector pCAMBIA-2300"
                     /mol_type="other DNA"
                     /db_xref="taxon:118407"
ORIGIN      
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gcaggcttaa ggccagtcac
     2701 aatgggggtt tcactggtgt gtcatgcaca tttaataggg gtaagactga ataaaaaatg
     2761 attatttgca tgaaatgggg atgagagaga aggaaagagt ttcatcctgg tgaaactcgt
     2821 cagcgtcgtt tccaagtcct cggtaacaga gcgaaacccc cgttgaggcc gattcgtttc
     2881 attcaccgga tctcttgcgt ccgcctccgc cgtgcgacct ctgcattctc aagcttcatc
     2941 agctggcttg tggggaccag acaaaaaagg aatggtgcag aattgttagg cgcacctacc
     3001 aaaagcatct ttgcctttat tgcaaagata aagcagattc ctctagtaca agtggggaac
     3061 aaaataacgt ggaaaagagc tgtcctgaca gcccactcac taatgcgtat gacgaacgca
     3121 gtgacgacca caaaagaatt ccgcgccgcg ccggattatg ggtacaaatg atcccagcaa
     3181 cttgtaccaa ttaaatgcta tgcatagtca tggaaacgtc aaagtgaaac ccctccactg
     3241 tggggattgt ttcataaaag atttcatttg aaagaagatg gtataatatt ttgggtagcc
     3301 gtgcaatgac actagccatt gtgactggcc taagacccag ctttcttgta caaagtggtg
     3361 ctcgagatgg tagatctgac tagtaaagga gaagaacttt tcactggagt tgtcccaatt
     3421 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     3481 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     3541 gttccgtggc caacacttgt cactactttc tcttatggtg ttcaatgctt ttcaagatac
     3601 ccagatcata tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag
     3661 gagaggacca tcttcttcaa ggacgacggg aactacaaga cacgtgctga agtcaagttt
     3721 gagggagaca ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga
     3781 aacatcctcg gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc
     3841 gacaagcaaa agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc
     3901 ggcgtgcaac tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     3961 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     4021 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     4081 gaactataca aagctagcca ccaccaccac caccacgtgt gacctaggtg agtctagaga
     4141 gttaattaag acccgggact agtccctaga gtcctgcttt aatgagatat gcgagacgcc
     4201 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg
     4261 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact
     4321 atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct actacttata
     4381 tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg
     4441 atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa
     4501 gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag
     4561 tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg
     4621 gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt
     4681 ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc
     4741 gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt
     4801 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
     4861 caacatgtcg aggctcagca ggacctgcag gcatgcaagc ttggcactgg ccgtcgtttt
     4921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5041 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
     5101 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     5161 gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     5221 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
     5281 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     5341 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     5401 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     5461 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     5521 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     5581 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     5641 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     5701 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     5761 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     5821 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     5881 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     5941 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     6001 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     6061 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     6121 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     6181 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     6241 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     6301 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     6361 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     6421 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     6481 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     6541 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     6601 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     6661 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     6721 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     6781 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     6841 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     6901 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     6961 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     7021 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     7081 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     7141 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     7201 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     7261 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     7321 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     7381 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     7441 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     7501 atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     7561 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     7621 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     7681 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     7741 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     7801 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     7861 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     7921 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     7981 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     8041 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     8101 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     8161 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     8221 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     8281 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     8341 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     8401 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     8461 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     8521 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     8581 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     8641 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     8701 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     8761 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     8821 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     8881 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     8941 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     9001 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     9061 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9121 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9181 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     9241 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9301 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10141 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
    10201 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
    10261 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
    10321 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
    10381 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
    10441 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
    10501 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
    10561 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
    10621 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
    10681 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
    10741 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
    10801 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
    10861 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
    10921 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
    10981 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    11041 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    11101 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    11161 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    11221 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    11281 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    11341 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    11401 gg
//
