LOCUS pAS_4xMmaPylT(UUA)_FLAG 9428 bp ds-DNA circular 13-FEB-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature 1938..2006 /label="PylT TAA" misc_feature 2353..2595 /label="h7SK promoter" misc_feature 6611..6918 /label="PB5'" misc_feature complement(7455..7485) /label="promoter for the E. coli lac operon" misc_feature 1695..1937 /label="h7SK promoter" CDS complement(8568..9428) /label="bla CDS" misc_feature 5999..6130 /label="SV40 EEL poly(A)" misc_feature complement(7809..8397) /label="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication" misc_feature 2024..2266 /label="h7SK promoter" CDS 3276..4664 /label="MmAcKRS3" misc_feature 727..744 /label="M13 uni (-21) (mismatches: 0)" misc_feature complement(6377..6605) /label="Core Insulator" misc_feature 2267..2335 /label="PylT TAA" misc_feature 4679..5272 /label="IRES" CDS 5285..5884 /label="pac from Streptomyces alboniger CDS" misc_feature complement(1..105) /label="bla Promoter" misc_feature 705..727 /label="M13 uni (-43) (mismatches: 0)" misc_feature 3242..3248 /label="MCS" misc_feature 1366..1608 /label="h7SK promoter" misc_feature 3276..3302 /label="FLAG" misc_feature complement(7465..7490) /label="pEX-Rev (mismatches: 0)" misc_feature complement(131..586) /label="f1 bacteriophage origin of replication" misc_feature complement(7406..7423) /label="M13 rev (-29) (mismatches: 0)" misc_feature 2596..2664 /label="PylT TAA" misc_feature 871..1110 /label="PB3'" misc_feature 1609..1677 /label="Pylt TAA" promoter 2696..3241 /label="EF1" misc_feature 1116..1344 /label="Core Insulator" ORIGIN 1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA 1081 AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA 1141 GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT 1201 CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG 1261 GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG 1321 ACACCTGGGG GGATACGGGG AAAAGGCCTC CACgGCCaaC TAGTTCTGCA GTATTTAGCA 1381 TGCCCCACCC ATCTGCAAGG CATTCTGGAT AGTGTCAAAA CAGCCGGAAA TCAAGTCCGT 1441 TTATCTCAAA CTTTAGCATT TTGGGAATAA ATGATATTTG CTATGCTGGT TAAATTAGAT 1501 TTTAGTTAAA TTTCCTGCTG AAGCTCTAGT ACGATAAGTA ACTTGACCTA AGTGTAAAGT 1561 TGAGATTTCC TTCAGGTTTA TATAGCTTGT GCGCCGCCTG GGTACCTCGG AAACCTGATC 1621 ATGTAGATCG AACGGACTTT AAATCCGTTC AGCCGGGTTA GATTCCCGGG GTTTCCGGAC 1681 AAGTGCGGTT TTTCCTGCAG TATTTAGCAT GCCCCACCCA TCTGCAAGGC ATTCTGGATA 1741 GTGTCAAAAC AGCCGGAAAT CAAGTCCGTT TATCTCAAAC TTTAGCATTT TGGGAATAAA 1801 TGATATTTGC TATGCTGGTT AAATTAGATT TTAGTTAAAT TTCCTGCTGA AGCTCTAGTA 1861 CGATAAGTAA CTTGACCTAA GTGTAAAGTT GAGATTTCCT TCAGGTTTAT ATAGCTTGTG 1921 CGCCGCCTGG GTACCTCGGA AACCTGATCA TGTAGATCGA ACGGACTTTA AATCCGTTCA 1981 GCCGGGTTAG ATTCCCGGGG TTTCCGGACA AGTGCGGTTT TTCCTGCAGT ATTTAGCATG 2041 CCCCACCCAT CTGCAAGGCA TTCTGGATAG TGTCAAAACA GCCGGAAATC AAGTCCGTTT 2101 ATCTCAAACT TTAGCATTTT GGGAATAAAT GATATTTGCT ATGCTGGTTA AATTAGATTT 2161 TAGTTAAATT TCCTGCTGAA GCTCTAGTAC GATAAGTAAC TTGACCTAAG TGTAAAGTTG 2221 AGATTTCCTT CAGGTTTATA TAGCTTGTGC GCCGCCTGGG TACCTCGGAA ACCTGATCAT 2281 GTAGATCGAA CGGACTTTAA ATCCGTTCAG CCGGGTTAGA TTCCCGGGGT TTCCGGACAA 2341 GTGCGGTTTT TCCTGCAGTA TTTAGCATGC CCCACCCATC TGCAAGGCAT TCTGGATAGT 2401 GTCAAAACAG CCGGAAATCA AGTCCGTTTA TCTCAAACTT TAGCATTTTG GGAATAAATG 2461 ATATTTGCTA TGCTGGTTAA ATTAGATTTT AGTTAAATTT CCTGCTGAAG CTCTAGTACG 2521 ATAAGTAACT TGACCTAAGT GTAAAGTTGA GATTTCCTTC AGGTTTATAT AGCTTGTGCG 2581 CCGCCTGGGT ACCTCGGAAA CCTGATCATG TAGATCGAAC GGACTTTAAA TCCGTTCAGC 2641 CGGGTTAGAT TCCCGGGGTT TCCGGACAAG TGCGGTTTTT CActagtgct ctagaaagga 2701 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 2761 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact 2821 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 2881 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc 2941 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc 3001 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt 3061 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt 3121 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct 3181 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta 3241 ctctagagCT AGCGTTTAAA CTTAAGCTTG CCACCATGGA CTACAAGGAC GACGACGACA 3301 AGATGGACAA GAAGCCCCTG AACACCCTGA TCAGCGCCAC AGGACTGTGG ATGTCCAGAA 3361 CCGGCACCAT CCACAAGATC AAGCACCACG AGGTGTCCCG GTCCAAAATC TACATCGAGA 3421 TGGCCTGCGG CGATCACCTG GTCGTCAACA ACAGCAGAAG CAGCCGGACA GCCAGAGCCC 3481 TGCGGCACCA CAAGTACAGA AAGACCTGCA AGCGGTGCAG AGTGTCCGAC GAGGACCTGA 3541 ACAAGTTCCT GACCAAGGCC AACGAGGACC AGACCAGCGT GAAAGTGAAG GTGGTGTCCG 3601 CCCCCACCCG GACCAAGAAA GCCATGCCCA AGAGCGTGGC CAGAGCCCCC AAGCCCCTGG 3661 AAAACACCGA AGCCGCTCAG GCCCAGCCCA GCGGCAGCAA GTTCAGCCCC GCCATCCCCG 3721 TGTCTACCCA GGAAAGCGTC AGCGTCCCCG CCAGCGTGTC CACCAGCATC TCTAGCATCT 3781 CAACCGGCGC CACAGCTTCT GCCCTGGTCA AGGGCAACAC CAACCCCATC ACCAGCATGT 3841 CTGCCCCTGT GCAGGCCTCT GCCCCAGCCC TGACCAAGTC CCAGACCGAC CGGCTGGAAG 3901 TGCTCCTGAA CCCCAAGGAC GAGATCAGCC TGAACAGCGG CAAGCCCTTC CGGGAGCTGG 3961 AAAGCGAGCT GCTGAGCCGG CGGAAGAAGG ACCTCCAGCA AATCTACGCC GAGGAACGGG 4021 AGAACTACCT GGGCAAGCTG GAAAGAGAGA TCACCCGGTT CTTCGTGGAC CGGGGCTTCC 4081 TGGAAATCAA GAGCCCCATC CTGATCCCCC TGGAGTACAT CGAGCGGATG GGCATCGACA 4141 ACGACACCGA GCTGAGCAAG CAGATTTTCC GGGTGGACAA GAACTTCTGC CTGCGGCCCA 4201 TGATGGCCCC CAACATCTTC AACTACGCCC GGAAACTGGA TCGCGCTCTG CCCGACCCCA 4261 TCAAGATTTT CGAGATCGGC CCCTGCTACC GGAAAGAGAG CGACGGCAAA GAGCACCTGG 4321 AAGAGTTTAC AATGCTGAAC TTTTTCCAGA TGGGCAGCGG CTGCACCAGA GAGAACCTGG 4381 AATCCATCAT CACCGACTTT CTGAACCACC TGGGGATCGA CTTCAAGATC GTGGGCGACA 4441 GCTGCATGGT GTACGGCGAC ACCCTGGACG TGATGCACGG CGACCTGGAA CTGTCTAGCG 4501 CCGTCGTGGG ACCCATCCCT CTGGACCGGG AGTGGGGCAT CGATAAGCCC TGGATCGGAG 4561 CCGGCTTCGG CCTGGAACGG CTGCTGAAAG TCAAGCACGA CTTTAAGAAC ATCAAGCGGG 4621 CTGCCAGAAG CGAGAGCTAC TACAACGGCA TCAGCACCAA CCTGTGATGA GGATCCGCGG 4681 CCGCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC 4741 CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG 4801 GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC 4861 AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA 4921 AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG 4981 TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG 5041 TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC 5101 AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT 5161 CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC 5221 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGCGTC 5281 CGGAATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC 5341 CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC 5401 GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT 5461 CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC 5521 GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG 5581 CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA 5641 GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT 5701 GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT 5761 CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC 5821 CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC 5881 CTGAGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 5941 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGTTAA 6001 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 6061 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 6121 TCATGTCTGG AATTGACTCA AATGATGTCA ATTAGTCTAT CAGAAGCTAT CTGGTCTCCC 6181 TTCCGGGGGA CAAGACATCC CTGTTTAATA TTTAAACAGC AGTGTTCCCA AACTGGGTTC 6241 TTATATCCCT TGCTCTGGTC AACCAGGTTG CAGGGTTTCC TGTCCTCACA GGAACGAAGT 6301 CCCTAAAGAA ACAGTGGCAG CCAGGTTTAG CCCCGGAATT GACTGGATTC CTTTTTTAGG 6361 GCCCATTGGT ATGGCTTTTT CCCCGTATCC CCCCAGGTGT CTGCAGGCTC AAAGAGCAGC 6421 GAGAAGCGTT CAGAGGAAAG CGATCCCGTG CCACCTTCCC CGTGCCCGGG CTGTCCCCGC 6481 ACGCTGCCGG CTCGGGGATG CGGGGGGAGC GCCGGACCGG AGCGGAGCCC CGGGCGGCTC 6541 GCTGCTGCCC CCTAGCGGGG GAGGGACGTA ATTACATCCC TGGGGGCTTT GGGGGGGGGC 6601 TGTCCCTGAT ATCTATAACA AGAAAATATA TATATAATAA GTTATCACGT AAGTAGAACA 6661 TGAAATAACA ATATAATTAT CGTATGAGTT AAATCTTAAA AGTCACGTAA AAGATAATCA 6721 TGCGTCATTT TGACTCACGC GGTCGTTATA GTTCAAAATC AGTGACACTT ACCGCATTGA 6781 CAAGCACGCC TCACGGGAGC TCCAAGCGGC GACTGAGATG TCCTAAATGC ACAGCGACGG 6841 ATTCGCGCTA TTTAGAAAGA GAGAGCAATA TTTCAAGAAT GCATGCGTCA ATTTTACGCA 6901 GACTATCTTT CTAGGGTTAA TCTAGCTGCA TCAGGATCAT ATCGTCGGGT CTTTTTTCCG 6961 GCTCAGTCAT CGCCCAAGCT GGCGCTATCT GGGCATCGGG GAGGAAGAAG CCCGTGCCTT 7021 TTCCCGCGAG GTTGAAGCGG CATGGAAAGA GTTTGCCGAG GATGACTGCT GCTGCATTGA 7081 CGTTGAGCGA AAACGCACGT TTACCATGAT GATTCGGGAA GGTGTGGCCA TGCACGCCTT 7141 TAACGGTGAA CTGTTCGTTC AGGCCACCTG GGATACCAGT TCGTCGCGGC TTTTCCGGAC 7201 ACAGTTCCGG ATGGTCAGCC CGAAGCGCAT CAGCAACCCG AACAATACCG GCGACAGCCG 7261 GAACTGCCGT GCCGGTGTGC AGATTAATGA CAGCGGTGCG GCGCTGGGAT ATTACGTCAG 7321 CGAGGACGGG TATCCTGGCT GGATGCCGCA GAAATGGACA TGGATACCCC GTGAGTTACC 7381 CGGCGGGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 7441 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 7501 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 7561 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 7621 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 7681 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 7741 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 7801 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 7861 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 7921 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 7981 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 8041 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 8101 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 8161 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 8221 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 8281 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 8341 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 8401 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 8461 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 8521 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 8581 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 8641 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 8701 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 8761 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 8821 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 8881 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 8941 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 9001 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 9061 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 9121 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 9181 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 9241 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 9301 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 9361 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 9421 ATACTCAT //