LOCUS pAS_4xBstTyrT(CUA)_EcoT 9576 bp ds-DNA circular 05-FEB-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature complement(4640..4655) /label="IRES_down" promoter 2764..3309 /label="EF1" misc_feature 4598..4621 /label="flag" misc_feature 4640..4649 /label="15mer BsrGI 3'" primer_bind 812..831 /label="BII(R)" misc_feature complement(4640..4649) /label="Seq_BsrGI_down" misc_feature complement(918..919) /label="3'TR" misc_feature 6141..6144 /label="SV40 pA" misc_feature 4640..4649 /label="BamHI(IRES)_15R" misc_feature complement(1725..1806) /label="BSt tRNA Tyr CUA" CDS 5230..6024 /label="aph(3')-II (or nptII)" CDS complement(8719..9576) /label="Amp" misc_feature 7030..7064 /label="5'TR" primer_bind complement(7583..7602) /label="pLac(F)" enhancer 1112..1327 /label="core insul" misc_feature 6150..6276 /label="SV40 pA" misc_feature complement(2497..2739) /label="h7SK promoter" misc_feature complement(1462..1704) /label="h7SK promoter" misc_feature 3326..4624 /label="AzF-RS1" misc_feature complement(2070..2151) /label="BSt tRNA Tyr CUA" misc_feature complement(921..936) /label="3'TR" misc_feature complement(1380..1461) /label="BSt tRNA Tyr CUA" misc_feature complement(2152..2394) /label="h7SK promoter" misc_feature complement(887..917) /label="3'TR" misc_feature 887..917 /label="31bp spacer" misc_feature 4642..5229 /label="IRES" misc_feature complement(1807..2049) /label="h7SK promoter" enhancer complement(6525..6755) /label="core insul" misc_feature complement(2415..2496) /label="BSt tRNA Tyr CUA" misc_feature complement(874..886) /label="3'TR" ORIGIN 1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA 1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG 1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC ACgGCCaact agTAAAAACC GCACTTGTCT 1381 GGAGGGGGAC GGATTCGAAC CGCCGAACCC AAAGGGAGCG GATTTAGAGT CCGCCGCGTT 1441 TAGCCACTTC GCTACCCCTC CGAGGTACCC AGGCGGCGCA CAAGCTATAT AAACCTGAAG 1501 GAAATCTCAA CTTTACACTT AGGTCAAGTT ACTTATCGTA CTAGAGCTTC AGCAGGAAAT 1561 TTAACTAAAA TCTAATTTAA CCAGCATAGC AAATATCATT TATTCCCAAA ATGCTAAAGT 1621 TTGAGATAAA CGGACTTGAT TTCCGGCTGT TTTGACACTA TCCAGAATGC CTTGCAGATG 1681 GGTGGGGCAT GCTAAATACT GCAGgcaaAA AAACCGCACT TGTCTGGAGG GGGACGGATT 1741 CGAACCGCCG AACCCAAAGG GAGCGGATTT AGAGTCCGCC GCGTTTAGCC ACTTCGCTAC 1801 CCCTCCGAGG TACCCAGGCG GCGCACAAGC TATATAAACC TGAAGGAAAT CTCAACTTTA 1861 CACTTAGGTC AAGTTACTTA TCGTACTAGA GCTTCAGCAG GAAATTTAAC TAAAATCTAA 1921 TTTAACCAGC ATAGCAAATA TCATTTATTC CCAAAATGCT AAAGTTTGAG ATAAACGGAC 1981 TTGATTTCCG GCTGTTTTGA CACTATCCAG AATGCCTTGC AGATGGGTGG GGCATGCTAA 2041 ATACTGCAGc aagAAAAACC GCACTTGTCT GGAGGGGGAC GGATTCGAAC CGCCGAACCC 2101 AAAGGGAGCG GATTTAGAGT CCGCCGCGTT TAGCCACTTC GCTACCCCTC CGAGGTACCC 2161 AGGCGGCGCA CAAGCTATAT AAACCTGAAG GAAATCTCAA CTTTACACTT AGGTCAAGTT 2221 ACTTATCGTA CTAGAGCTTC AGCAGGAAAT TTAACTAAAA TCTAATTTAA CCAGCATAGC 2281 AAATATCATT TATTCCCAAA ATGCTAAAGT TTGAGATAAA CGGACTTGAT TTCCGGCTGT 2341 TTTGACACTA TCCAGAATGC CTTGCAGATG GGTGGGGCAT GCTAAATACT GCAGcgatAA 2401 AAACCGCACT TGTCTGGAGG GGGACGGATT CGAACCGCCG AACCCAAAGG GAGCGGATTT 2461 AGAGTCCGCC GCGTTTAGCC ACTTCGCTAC CCCTCCGAGG TACCCAGGCG GCGCACAAGC 2521 TATATAAACC TGAAGGAAAT CTCAACTTTA CACTTAGGTC AAGTTACTTA TCGTACTAGA 2581 GCTTCAGCAG GAAATTTAAC TAAAATCTAA TTTAACCAGC ATAGCAAATA TCATTTATTC 2641 CCAAAATGCT AAAGTTTGAG ATAAACGGAC TTGATTTCCG GCTGTTTTGA CACTATCCAG 2701 AATGCCTTGC AGATGGGTGG GGCATGCTAA ATACTGCAGC TCCAAGGCCA ctagtgctct 2761 agaaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 2821 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 2881 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 2941 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 3001 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 3061 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 3121 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 3181 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 3241 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 3301 ggcgcctact ctagagctag cCACCATGGC AAGCAGTAAC TTGATTAAAC AATTGCAAGA 3361 GCGGGGGCTG GTAGCCCAGG TGACGGACGA GGAAGCGTTA GCAGAGCGAC TGGCGCAAGG 3421 CCCGATCGCG CTCCTGTGCG GCTTCGATCC TACCGCTGAC AGCTTGCATT TGGGGCATCT 3481 TGTTCCATTG TTATGCCTGA AACGCTTCCA GCAGGCGGGC CACAAGCCGG TTGCGCTGGT 3541 AGGCGGCGCG ACGGGTCTGA TTGGCGACCC GAGCTTCAAA GCTGCCGAGC GTAAGCTGAA 3601 CACCGAAGAA ACTGTTCAGG AGTGGGTGGA CAAAATCCGT AAGCAGGTTG CCCCGTTCCT 3661 CGATTTCGAC TGTGGAGAAA ACTCTGCTAT CGCGGCGAAC AACTATGACT GGTTCGGCAA 3721 TATGAATGTG CTGACCTTCC TGCGCGATAT TGGCAAACAC TTCTCCGTTA ACCAGATGAT 3781 CAACAAAGAA GCGGTTAAGC AGCGTCTCAA CCGTGAAGAT CAGGGGATTT CGTTCACTGA 3841 GTTTTCCTAC AACCTGTTGC AGGGTTATAG CATGGCCTGT GCGAACAAAC AGTACGGTGT 3901 GGTGCTGCAA ATTGGTGGTT CTGACCAGTG GGGTAACATC ACTTCTGGTA TCGACCTGAC 3961 CCGTCGTCTG CATCAGAATC AGGTGTTTGG CCTGACCGTT CCGCTGATCA CTAAAGCAGA 4021 TGGCACCAAA TTTGGTAAAA CTGAAGGCGG CGCAGTCTGG TTGGAcCCGA AGAAAACCAG 4081 CCCGTACAAA TTCTACCAGT TCTGGATCAA CACTGCGGAT GCCGACGTTT ACCGCTTCCT 4141 GAAGTTCTTC ACCTTTATGA GCATTGAAGA GATCAACGCC CTGGAAGAAG AAGATAAAAA 4201 CAGCGGTAAA GCACCGCGCG CCCAGTATGT ACTGGCGGAG CAGGTGACTC GTCTGGTTCA 4261 CGGTGAAGAA GGTTTACAGG CGGCAAAACG TATTACCGAA TGCCTGTTCA GCGGTTCTTT 4321 GAGTGCGCTG AGTGAAGCGG ACTTCGAACA GCTGGCGCAG GACGGCGTAC CGATGGTTGA 4381 GATGGAAAAG GGCGCAGACC TGATGCAGGC ACTGGTCGAT TCTGAACTGC AACCTTCCCG 4441 TGGTCAGGCA CGTAAAACTA TCGCCTCCAA TGCCATCACC ATTAACGGTG AAAAACAGTC 4501 CGATCCTGAA TACTTCTTTA AAGAAGAAGA TCGTCTGTTT GGTCGTTTTA CCTTACTGCG 4561 TCGCGGTAAA AAGAATTACT GTCTGATTTG CTGGAAAGAC TACAAAGACG ATGACGACAA 4621 GTgATGAATC GATGGATCCG CGGCCGCGCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG 4681 GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT 4741 TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC 4801 CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG 4861 CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC 4921 GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC 4981 CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA 5041 AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT 5101 GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA 5161 AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA ACACGATAAT 5221 ACCTCCGGAA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 5281 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 5341 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 5401 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 5461 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 5521 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 5581 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 5641 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 5701 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 5761 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 5821 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 5881 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 5941 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 6001 CGCCTTCTTG ACGAGTTCTT CTGAGTCGAC aatcaacctc tggattacaa aatttgtgaa 6061 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 6121 atgcctttgt atcatgcGTT AACTaaactT GTTTATTGCA GCTTATAATG GTTACAAATA 6181 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 6241 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGAAT TGACTCAAAT GATGTCAATT 6301 AGTCTATCAG AAGCTCATCT GGTCTCCCTT CCGGGGGACA AGACATCCCT GTTTAATATT 6361 TAAACAGCAG TGTTCCCAAA CTGGGTTCTT ATATCCCTTG CTCTGGTCAA CCAGGTTGCA 6421 GGGTTTCCTG TCCTCACAGG AACGAAGTCC CTAAAGAAAC AGTGGCAGCC AGGTTTAGCC 6481 CCGGAATTGA CTGGATTCCT TTTTTAGGGC CCATTGGTAT GGCTTTTTCC CCGTATCCCC 6541 CCAGGTGTCT GCAGGCTCAA AGAGCAGCGA GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC 6601 ACCTTCCCCG TGCCCGGGCT GTCCCCGCAC GCTGCCGGCT CGGGGATGCG GGGGGAGCGC 6661 CGGACCGGAG CGGAGCCCCG GGCGGCTCGC TGCTGCCCCC TAGCGGGGGA GGGACGTAAT 6721 TACATCCCTG GGGGCTTTGG GGGGGGGCTG TCCCTGATAT CTATAACAAG AAAATATATA 6781 TATAATAAGT TATCACGTAA GTAGAACATG AAATAACAAT ATAATTATCG TATGAGTTAA 6841 ATCTTAAAAG TCACGTAAAA GATAATCATG CGTCATTTTG ACTCACGCGG TCGTTATAGT 6901 TCAAAATCAG TGACACTTAC CGCATTGACA AGCACGCCTC ACGGGAGCTC CAAGCGGCGA 6961 CTGAGATGTC CTAAATGCAC AGCGACGGAT TCGCGCTATT TAGAAAGAGA GAGCAATATT 7021 TCAAGAATGC ATGCGTCAAT TTTACGCAGA CTATCTTTCT AGGGTTAATC TAGCTGCATC 7081 AGGATCATAT CGTCGGGTCT TTTTTCCGGC TCAGTCATCG CCCAAGCTGG CGCTATCTGG 7141 GCATCGGGGA GGAAGAAGCC CGTGCCTTTT CCCGCGAGGT TGAAGCGGCA TGGAAAGAGT 7201 TTGCCGAGGA TGACTGCTGC TGCATTGACG TTGAGCGAAA ACGCACGTTT ACCATGATGA 7261 TTCGGGAAGG TGTGGCCATG CACGCCTTTA ACGGTGAACT GTTCGTTCAG GCCACCTGGG 7321 ATACCAGTTC GTCGCGGCTT TTCCGGACAC AGTTCCGGAT GGTCAGCCCG AAGCGCATCA 7381 GCAACCCGAA CAATACCGGC GACAGCCGGA ACTGCCGTGC CGGTGTGCAG ATTAATGACA 7441 GCGGTGCGGC GCTGGGATAT TACGTCAGCG AGGACGGGTA TCCTGGCTGG ATGCCGCAGA 7501 AATGGACATG GATACCCCGT GAGTTACCCG GCGGGCGCGC TTGGCGTAAT CATGGTCATA 7561 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 7621 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 7681 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 7741 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 7801 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 7861 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 7921 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 7981 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 8041 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 8101 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 8161 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 8221 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 8281 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 8341 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 8401 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 8461 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 8521 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 8581 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 8641 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 8701 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 8761 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 8821 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 8881 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 8941 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 9001 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 9061 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 9121 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 9181 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 9241 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 9301 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 9361 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 9421 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 9481 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 9541 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCAT //