LOCUS pAS_4xMmaPylT_FLAG-AbKR 9305 bp ds-DNA circular 13-FEB-2020 DEFINITION . FEATURES Location/Qualifiers promoter complement(1451..1693) /label="h7SK promoter" promoter 2696..3241 /label="EF1" misc_feature 5967..5986 /label="pCEP-Reverse (mismatches: 0)" promoter complement(2109..2351) /label="h7SK promoter" misc_feature complement(7686..8274) /label="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication" misc_feature complement(2369..2437) /label="PylT" misc_feature complement(6254..6482) /label="Core Insulator" CDS 6983..7354 /label="Length: 372;Found at strand: positive;Start codon:ATG" misc_feature 727..744 /label="M13 uni (-21) (mismatches: 0)" misc_feature complement(2024..2028) /label="U6 terminator" misc_feature complement(7283..7300) /label="M13 rev (-29) (mismatches: 0)" promoter complement(1780..2022) /label="h7SK promoter" misc_feature complement(1366..1370) /label="U6 terminator" misc_feature complement(1382..1450) /label="PylT" CDS 5162..5761 /label="pac from Streptomyces alboniger CDS" misc_feature complement(7332..7362) /label="promoter for the E. coli lac operon" promoter complement(2438..2680) /label="h7SK promoter" misc_feature 5876..6007 /label="SV40 EEL poly(A)" misc_feature complement(1..105) /label="bla Promoter" misc_feature 705..727 /label="M13 uni (-43) (mismatches: 0)" misc_feature 4556..5149 /label="IRES" misc_feature complement(2353..2357) /label="U6 terminator" CDS complement(8445..9305) /label="bla CDS" misc_feature complement(131..586) /label="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis" misc_feature 7377..7398 /label="CAP binding activates transcription in the presenceof cAMP." misc_feature complement(7342..7367) /label="pEX-Rev (mismatches: 0)" misc_feature 3242..3248 /label="MCS" misc_feature complement(7284..7300) /label="common sequencing primer, one of multiple similarvariants" misc_feature 728..744 /label="common sequencing primer, one of multiple similarvariants" misc_feature complement(1695..1699) /label="U6 terminator" misc_feature 6488..6795 /label="PB5'" misc_feature complement(2040..2108) /label="PylT" misc_feature complement(1711..1779) /label="PylT" misc_feature 3261..3284 /label="FLAG" misc_feature 871..1110 /label="PB3'" misc_feature 3258..4547 /label="AbKRS_chIPYE" misc_feature 1116..1344 /label="Core Insulator" misc_feature 7308..7324 /label="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition canberelieved by adding lactoseorisopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA 1081 AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA 1141 GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT 1201 CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG 1261 GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG 1321 ACACCTGGGG GGATACGGGG AAAAGGCCTC CACgGCCaac tagtgaaaaa ccgcacttgt 1381 ccggaaaccc cgggaatcta acccggctga acggatttag agtccgttcg atctacatga 1441 tcaggtttcc gaggtaccca ggcggcgcac aagctatata aacctgaagg aaatctcaac 1501 tttacactta ggtcaagtta cttatcgtac tagagcttca gcaggaaatt taactaaaat 1561 ctaatttaac cagcatagca aatatcattt attcccaaaa tgctaaagtt tgagataaac 1621 ggacttgatt tccggctgtt ttgacactat ccagaatgcc ttgcagatgg gtggggcatg 1681 ctaaatactg caggaaaaac cgcacttgtc cggaaacccc gggaatctaa cccggctgaa 1741 cggatttaga gtccgttcga tctacatgat caggtttccg aggtacccag gcggcgcaca 1801 agctatataa acctgaagga aatctcaact ttacacttag gtcaagttac ttatcgtact 1861 agagcttcag caggaaattt aactaaaatc taatttaacc agcatagcaa atatcattta 1921 ttcccaaaat gctaaagttt gagataaacg gacttgattt ccggctgttt tgacactatc 1981 cagaatgcct tgcagatggg tggggcatgc taaatactgc aggaaaaacc gcacttgtcc 2041 ggaaaccccg ggaatctaac ccggctgaac ggatttagag tccgttcgat ctacatgatc 2101 aggtttccga ggtacccagg cggcgcacaa gctatataaa cctgaaggaa atctcaactt 2161 tacacttagg tcaagttact tatcgtacta gagcttcagc aggaaattta actaaaatct 2221 aatttaacca gcatagcaaa tatcatttat tcccaaaatg ctaaagtttg agataaacgg 2281 acttgatttc cggctgtttt gacactatcc agaatgcctt gcagatgggt ggggcatgct 2341 aaatactgca ggaaaaaccg cacttgtccg gaaaccccgg gaatctaacc cggctgaacg 2401 gatttagagt ccgttcgatc tacatgatca ggtttccgag gtacccaggc ggcgcacaag 2461 ctatataaac ctgaaggaaa tctcaacttt acacttaggt caagttactt atcgtactag 2521 agcttcagca ggaaatttaa ctaaaatcta atttaaccag catagcaaat atcatttatt 2581 cccaaaatgc taaagtttga gataaacgga cttgatttcc ggctgttttg acactatcca 2641 gaatgccttg cagatgggtg gggcatgcta aatactgcag aactagtgct ctagaaagga 2701 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 2761 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact 2821 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 2881 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc 2941 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc 3001 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt 3061 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt 3121 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct 3181 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta 3241 ctctagagCT AGCCACCATG GACTACAAGG ACGACGACGA CAAGATGGAC AAGAAACCCC 3301 TGGACGTGCT GATCTCTGCC ACCGGCCTTT GGATGAGCAG AACCGGCACA CTGCACAAGA 3361 TCAAGCACTA CGAGATCAGC CGGTCCAAGA TCTACATCGA GATGGCCTGC GGCGACCACC 3421 TGGTCGTGAA CAACAGCAGA AGCTGCAGAC CCGCCAGAGC CTTCAGATAC CACAAGTACA 3481 GAAAGACCTG CAAGCGGTGC AGAGTGTCCG GCGAGGACAT CAACAACTTT CTGACCAGAA 3541 GCACCGAGGG CAAGACCAGC GTGAAAGTGA AGGTGGTGTC CGAGCCTAAA GTGAAGAAAG 3601 CCATGCCTAA GAGCGTGTCC AGGGCTCCCA AGCCTCTGGA AAATCCTGTG TCTGCCAAGG 3661 CCAGCACCGA CACCTCTAGA AGCGTTCCAT CTCCTGCCAA GAGCACCCCT AACAGCCCTG 3721 TGCCTACAAG CGCCTCTGCC CCAGCCCTGA CCAAGTCCCA GACCGACCGG CTGGAAGTGC 3781 TCCTGAACCC CAAGGACGAG ATCAGCCTGA ACAGCGGCAA GCCCTTCCGG GAGCTGGAAA 3841 GCGAGCTGCT GAGCCGGCGG AAGAAGGACC TCCAGCAAAT CTACGCCGAG GAACGGGAGA 3901 ACTACCTGGG CAAGCTGGAA AGAGAGATCA CCCGGTTCTT CGTGGACCGG GGCTTCCTGG 3961 AAATCAAGAG CCCCATCCTG ATCCCCCTGG AGTACATCGA GCGGATGGGC ATCGACAACG 4021 ACACCGAGCT GAGCAAGCAG ATTTTCCGGG TGGACAAGAA CTTCTGCCTG CGGCCCATGC 4081 TGGCCCCCAA CCTGTACAAC TACatgCGGA AACTGGATCG CGCTCTGCCC GACCCCATCA 4141 AGATTTTCGA GATCGGCCCC TGCTACCGGA AAGAGAGCGA CGGCAAAGAG CACCTGGAAG 4201 AGTTTACAAT GCTGAACTTT gccCAGATGG GCAGCGGCTG CACCAGAGAG AACCTGGAAT 4261 CCATCATCAC CGACTTTCTG AACCACCTGG GGATCGACTT CAAGATCGTG GGCGACAGCT 4321 GCATGGTGtt cGGCGACACC CTGGACGTGA TGCACGGCGA CCTGGAACTG TCTAGCGCCG 4381 TCGTGGGACC CATCCCTCTG GACCGGGAGT GGGGCATCGA TAAGCCCTGG ATCGGAGCCG 4441 GCTTCGGCCT GGAACGGCTG CTGAAAGTCA AGCACGACTT TAAGAACATC AAGCGGGCTG 4501 CCAGAAGCGA GAGCTACTAC AACGGCATCA GCACCAACCT GTGATGAGGA TCCGCGGCCG 4561 CGCCCCTCTC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG 4621 TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC 4681 CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA 4741 GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA 4801 CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC 4861 CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC 4921 CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC 4981 AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG 5041 TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC 5101 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCA TGGCGTCCGG 5161 AATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT 5221 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA 5281 CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA 5341 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA 5401 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG 5461 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA 5521 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG 5581 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT 5641 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC 5701 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTg 5761 aGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 5821 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGTTAACTT 5881 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 5941 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 6001 TGTCTGGAAT TGACTCAAAT GATGTCAATT AGTCTATCAG AAGCTATCTG GTCTCCCTTC 6061 CGGGGGACAA GACATCCCTG TTTAATATTT AAACAGCAGT GTTCCCAAAC TGGGTTCTTA 6121 TATCCCTTGC TCTGGTCAAC CAGGTTGCAG GGTTTCCTGT CCTCACAGGA ACGAAGTCCC 6181 TAAAGAAACA GTGGCAGCCA GGTTTAGCCC CGGAATTGAC TGGATTCCTT TTTTAGGGCC 6241 CATTGGTATG GCTTTTTCCC CGTATCCCCC CAGGTGTCTG CAGGCTCAAA GAGCAGCGAG 6301 AAGCGTTCAG AGGAAAGCGA TCCCGTGCCA CCTTCCCCGT GCCCGGGCTG TCCCCGCACG 6361 CTGCCGGCTC GGGGATGCGG GGGGAGCGCC GGACCGGAGC GGAGCCCCGG GCGGCTCGCT 6421 GCTGCCCCCT AGCGGGGGAG GGACGTAATT ACATCCCTGG GGGCTTTGGG GGGGGGCTGT 6481 CCCTGATATC TATAACAAGA AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA 6541 AATAACAATA TAATTATCGT ATGAGTTAAA TCTTAAAAGT CACGTAAAAG ATAATCATGC 6601 GTCATTTTGA CTCACGCGGT CGTTATAGTT CAAAATCAGT GACACTTACC GCATTGACAA 6661 GCACGCCTCA CGGGAGCTCC AAGCGGCGAC TGAGATGTCC TAAATGCACA GCGACGGATT 6721 CGCGCTATTT AGAAAGAGAG AGCAATATTT CAAGAATGCA TGCGTCAATT TTACGCAGAC 6781 TATCTTTCTA GGGTTAATCT AGCTGCATCA GGATCATATC GTCGGGTCTT TTTTCCGGCT 6841 CAGTCATCGC CCAAGCTGGC GCTATCTGGG CATCGGGGAG GAAGAAGCCC GTGCCTTTTC 6901 CCGCGAGGTT GAAGCGGCAT GGAAAGAGTT TGCCGAGGAT GACTGCTGCT GCATTGACGT 6961 TGAGCGAAAA CGCACGTTTA CCATGATGAT TCGGGAAGGT GTGGCCATGC ACGCCTTTAA 7021 CGGTGAACTG TTCGTTCAGG CCACCTGGGA TACCAGTTCG TCGCGGCTTT TCCGGACACA 7081 GTTCCGGATG GTCAGCCCGA AGCGCATCAG CAACCCGAAC AATACCGGCG ACAGCCGGAA 7141 CTGCCGTGCC GGTGTGCAGA TTAATGACAG CGGTGCGGCG CTGGGATATT ACGTCAGCGA 7201 GGACGGGTAT CCTGGCTGGA TGCCGCAGAA ATGGACATGG ATACCCCGTG AGTTACCCGG 7261 CGGGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 7321 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 7381 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 7441 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 7501 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 7561 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 7621 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 7681 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 7741 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 7801 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 7861 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 7921 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 7981 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 8041 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 8101 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 8161 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 8221 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 8281 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 8341 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 8401 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 8461 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 8521 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 8581 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 8641 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 8701 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 8761 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 8821 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 8881 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 8941 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 9001 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 9061 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 9121 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 9181 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 9241 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 9301 CTCAT //