LOCUS [pSW036]_pUC19-SSW07-ac 5144 bp ds-DNA circular 30-OCT-2018 DEFINITION . KEYWORDS "creator:New England Biolabs" "marker:AmpR" "organism:Escherichia coli" "ref:pmid\:2985470" FEATURES Location/Qualifiers misc_feature 2336..3049 /label="acsA upstream" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 CDS complement(880..1671) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 3164..3169 /label="RBS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind complement(4863..4879) /label="M13 rev" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 CDS complement(1672..1740) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 4052..4070 /label="T7_term" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3050..4129 /label="PCR from pSJ141" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 protein_bind 4887..4903 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b rep_origin complement(121..709) /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter complement(1741..1845) /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3178..3978 /label="YFP" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter complement(4911..4917) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3050..3138 /label="P_cpt" /ApEinfo_revcolor=#008000 /ApEinfo_fwdcolor=#008000 promoter complement(4918..4935) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 2336..4862 /label="acsA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 4130..4862 /label="acsA downstream" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter complement(4936..4941) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 2319..2335 /label="M13 fwd" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 ORIGIN 1 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 61 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 121 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 181 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 241 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 301 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 361 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 421 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 481 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 541 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 601 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 661 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 721 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 781 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 841 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 901 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 961 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 1021 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 1081 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 1141 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 1201 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 1261 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 1321 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 1381 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 1441 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 1501 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 1561 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 1621 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 1681 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 1741 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 1801 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 1861 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG 1921 TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 1981 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 2041 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 2101 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 2161 AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC 2221 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT 2281 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTgcaat 2341 gctgagatga tcctcggtga actccttgat cgccaagcct ttggtgtggt gcaaccgtta 2401 ctgtttgatc cagcgatcgc cccggagaat gaaaccattg cttttttacg gggacgctat 2461 atctggcaaa caaccttggc cgccgtccca ccatcgccaa cccaagcaac ggcgatcgcc 2521 acggtgcaag aaaattggga catggcccta gcccaacagc ccaacgaccc cgaaattttg 2581 atggccctgg gttttgtcca ttacctcaat gcagattacg aagccgccaa tgatcgttgg 2641 tatgaagcct tagcagcggc aacgacccag gatttccccc cagtcctcca ggcggagatt 2701 tatgcgggcc tggcgattag tatgtacgat ttggcccaag ggcaacccac cgacgttgac 2761 caggaaattc ttgtggggaa agccgaaaaa ctccgggcga tcgccctaga tctcgatcgt 2821 tcccagctca acccccgccg cctcgaacaa agttggttat ggttccctga ggcgatcgcc 2881 acttggcaaa ccctgctcgc tttagaagaa tctccctagg tcatatccga ggcgtacatt 2941 caatcaactt gagcgcaaaa atcttgcaaa ccattacggc ttaagcacga gaccccctga 3001 tggggcggac ttgggggaca atggggtgaa ataaatccca cgataataat taacaaaaaa 3061 gcaggaataa aattaacaag atgtaacaga cataagtccc atcaccgttg tataaagtta 3121 actgtgggat tgcaaaagga gctcccaact cataaagtca agtaggagat taattccatg 3181 ggccatcatc atcatcatca tcatcatcat cacagcagcg gccatatcga aggtcgtcat 3241 atgcaggcag cagcctcgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 3301 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 3361 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 3421 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc 3481 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3541 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3601 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3661 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3721 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3781 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 3841 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 3901 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 3961 gacgagctgt acaagtaacg agggcggtgc tttggcagga tccggctgtc taacaaagcc 4021 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg 4081 gcctctaaac gggtcttgac gggttttttg tctagatcaa cggcctcaaa gatgtccaga 4141 ggacagaggg tgtcttccct tgaggggagt cggagggaaa atttccatta ggtgtccctc 4201 cgactgcttg ccttgaccta gttcccttcg ataaaggcga aacggccctg ttgacccgat 4261 tcatccattt caatttgggc cacaaaaaat tccttctgga caatttcccc ttcctctgtg 4321 aaggaaattt cacccaaagg cgtgacgtag gtacctgcaa aaatttcgtc ccgcagttgt 4381 cgtcgcaagt ccggtagagc aagagtttct aggggcgttt tttcgtcgag gctgctgagg 4441 gcttcaacaa aaacttggat cgccgtaaaa gcctgggcac taaattgggg cggctctttt 4501 tggttttgtt gaaaataggc gtcccgaaac gcgcggttaa tctcattatc taactcggca 4561 ctgtaggctt gggccaccaa caccccatca cattttgctt ggcagacggg gaaaatatta 4621 gaagtattta agccgttccc cccaacaata atgccgttgt aacctaattc tcgcagttgc 4681 ttcaccaggt tgcccgcatc ggctgctaac cccgaaataa tcactagatc tggttgaaca 4741 ttgagtgcgg cagtcacctg ggcttgaaaa tcagtgtcgg tggtttgaaa cctttgtacc 4801 gtgactaatt ccagaccaaa atcttggaca gccgtctgga aggtttctgt ctctgattta 4861 ctGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4921 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4981 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 5041 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 5101 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATC 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