LOCUS       [pSW036]_pUC19-SSW07-ac 5144 bp ds-DNA     circular     30-OCT-2018
DEFINITION  .
KEYWORDS    "creator:New England Biolabs" "marker:AmpR" "organism:Escherichia  
            coli" "ref:pmid\:2985470"
FEATURES             Location/Qualifiers
     misc_feature    2336..3049
                     /label="acsA upstream"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(880..1671)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3164..3169
                     /label="RBS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(4863..4879)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     CDS             complement(1672..1740)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4052..4070
                     /label="T7_term"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3050..4129
                     /label="PCR from pSJ141"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    4887..4903
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     rep_origin      complement(121..709)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(1741..1845)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3178..3978
                     /label="YFP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(4911..4917)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        3050..3138
                     /label="P_cpt"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     promoter        complement(4918..4935)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2336..4862
                     /label="acsA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4130..4862
                     /label="acsA downstream"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(4936..4941)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     2319..2335
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
ORIGIN
        1 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
       61 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
      121 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
      181 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
      241 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
      301 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
      361 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
      421 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
      481 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
      541 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
      601 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
      661 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
      721 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
      781 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
      841 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
      901 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
      961 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     1021 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     1081 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     1141 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     1201 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     1261 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     1321 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     1381 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     1441 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     1501 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     1561 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     1621 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     1681 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     1741 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     1801 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     1861 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG
     1921 TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT
     1981 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG
     2041 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA
     2101 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG
     2161 AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC
     2221 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT
     2281 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTgcaat
     2341 gctgagatga tcctcggtga actccttgat cgccaagcct ttggtgtggt gcaaccgtta
     2401 ctgtttgatc cagcgatcgc cccggagaat gaaaccattg cttttttacg gggacgctat
     2461 atctggcaaa caaccttggc cgccgtccca ccatcgccaa cccaagcaac ggcgatcgcc
     2521 acggtgcaag aaaattggga catggcccta gcccaacagc ccaacgaccc cgaaattttg
     2581 atggccctgg gttttgtcca ttacctcaat gcagattacg aagccgccaa tgatcgttgg
     2641 tatgaagcct tagcagcggc aacgacccag gatttccccc cagtcctcca ggcggagatt
     2701 tatgcgggcc tggcgattag tatgtacgat ttggcccaag ggcaacccac cgacgttgac
     2761 caggaaattc ttgtggggaa agccgaaaaa ctccgggcga tcgccctaga tctcgatcgt
     2821 tcccagctca acccccgccg cctcgaacaa agttggttat ggttccctga ggcgatcgcc
     2881 acttggcaaa ccctgctcgc tttagaagaa tctccctagg tcatatccga ggcgtacatt
     2941 caatcaactt gagcgcaaaa atcttgcaaa ccattacggc ttaagcacga gaccccctga
     3001 tggggcggac ttgggggaca atggggtgaa ataaatccca cgataataat taacaaaaaa
     3061 gcaggaataa aattaacaag atgtaacaga cataagtccc atcaccgttg tataaagtta
     3121 actgtgggat tgcaaaagga gctcccaact cataaagtca agtaggagat taattccatg
     3181 ggccatcatc atcatcatca tcatcatcat cacagcagcg gccatatcga aggtcgtcat
     3241 atgcaggcag cagcctcgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3301 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3361 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3421 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
     3481 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3541 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3601 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3661 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3721 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     3781 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3841 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     3901 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3961 gacgagctgt acaagtaacg agggcggtgc tttggcagga tccggctgtc taacaaagcc
     4021 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     4081 gcctctaaac gggtcttgac gggttttttg tctagatcaa cggcctcaaa gatgtccaga
     4141 ggacagaggg tgtcttccct tgaggggagt cggagggaaa atttccatta ggtgtccctc
     4201 cgactgcttg ccttgaccta gttcccttcg ataaaggcga aacggccctg ttgacccgat
     4261 tcatccattt caatttgggc cacaaaaaat tccttctgga caatttcccc ttcctctgtg
     4321 aaggaaattt cacccaaagg cgtgacgtag gtacctgcaa aaatttcgtc ccgcagttgt
     4381 cgtcgcaagt ccggtagagc aagagtttct aggggcgttt tttcgtcgag gctgctgagg
     4441 gcttcaacaa aaacttggat cgccgtaaaa gcctgggcac taaattgggg cggctctttt
     4501 tggttttgtt gaaaataggc gtcccgaaac gcgcggttaa tctcattatc taactcggca
     4561 ctgtaggctt gggccaccaa caccccatca cattttgctt ggcagacggg gaaaatatta
     4621 gaagtattta agccgttccc cccaacaata atgccgttgt aacctaattc tcgcagttgc
     4681 ttcaccaggt tgcccgcatc ggctgctaac cccgaaataa tcactagatc tggttgaaca
     4741 ttgagtgcgg cagtcacctg ggcttgaaaa tcagtgtcgg tggtttgaaa cctttgtacc
     4801 gtgactaatt ccagaccaaa atcttggaca gccgtctgga aggtttctgt ctctgattta
     4861 ctGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
     4921 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
     4981 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA
     5041 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
     5101 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATC
//