LOCUS [pSW068]_pUC-SSW07-fadD 7480 bp ds-DNA circular 08-MAY-2019 DEFINITION . KEYWORDS "creator:New England Biolabs" "marker:AmpR" "organism:Escherichia coli" "ref:pmid\:2985470" FEATURES Location/Qualifiers gene 1295..1362 /label="lacUV5" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 promoter complement(7281..7385) /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 3540..4349 /label="KanR" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 misc_feature 2238..2243 /label="-10" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 2215..2249 /label="pMB2 Promoter" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2215..2220 /label="-35" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 protein_bind 5283..5299 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b -10_signal 1355..1361 /label="-10" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 promoter complement(5314..5331) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 -35_signal 1332..1337 /label="-35" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 rep_origin complement(5661..6249) /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 1416..1421 /label="RBS" /ApEinfo_revcolor=#0080ff /ApEinfo_fwdcolor=#0080ff gene 1430..1978 /label="'tesA_CO" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2936..2936 /label="W220" /ApEinfo_revcolor=#0080ff /ApEinfo_fwdcolor=#0080ff promoter complement(5307..5313) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 1295..1362 /label="Promoter_P_1" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 CDS complement(7212..7280) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1332..1333 /label="CA->TT Mutation in cLac143" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 2059..2077 /label="T7 terminator" /ApEinfo_revcolor=#0080ff /ApEinfo_fwdcolor=#0080ff promoter complement(5332..5337) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 379..395 /label="M13 fwd" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 misc_feature 1362..1396 /label="Lac Operator" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(5259..5275) /label="M13 rev" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 misc_feature 396..1248 /label="fadD upstream" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 396..5258 /label="fadD region" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 1295..1361 /label="Promoter" /ApEinfo_revcolor=#0080ff /ApEinfo_fwdcolor=#0080ff gene 2267..3358 /label="LacI" /ApEinfo_revcolor=#0080ff /ApEinfo_fwdcolor=#0080ff misc_feature 3429..3456 /label="rrnB T2 from Melamine plasmid" /ApEinfo_revcolor=#808080 /ApEinfo_fwdcolor=#808080 misc_feature 1265..1288 /label="LacOperator" /ApEinfo_revcolor=#0080ff /ApEinfo_fwdcolor=#0080ff CDS complement(6420..7211) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1985..2006 /label="rrnB T2 from Melamine plasmid" /ApEinfo_revcolor=#808080 /ApEinfo_fwdcolor=#808080 misc_feature 1249..3462 /label="clac143 tesA" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 4350..5258 /label="fadD downstream" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 1368..1368 /label="Predicted Transcription Start Site" /ApEinfo_revcolor=#0080ff /ApEinfo_fwdcolor=#0080ff ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTtaatt aaaatttgac tgcgatcgcc 421 tgcaatggcc tgggcctggg cgacaatttt cgggctaggt tcgtaatctt tgggggtagc 481 gatgcgaata ttcatgccga ctaacgcgcc accgaggagg atcgagtggg cgacattatt 541 gccatcaccc acataggtca tggtcaggcc ttcgagttgg ccgaaggttt cctgcatcgt 601 ttgcagatcc gccaacatct gacaggggtg ttctaaatct gtaagggcgt tgataatcgg 661 catttcacag taatcagcga aggtttcgag atcctgttga tcaaaggtac gaatcgccaa 721 aatatcgaga tagcggtcta aaaccctggc ggtgtcctcg atgggctcgc cgcgacccac 781 ttgggtacgg tttgggttca ggtcaatgac attgccaccc agttggtaca tggccacgct 841 aaaggagact cgggtgcggg tagaggcttt gtaaaataac agaccgaggg ttttggggca 901 ggtgggggcg atttttccct gtttgagatc tgccgccagg tttagcaggg taataatttc 961 tgtgctgctg agatctgcaa tgctcagaag atcccgaccc ttgagtgcat tcatcctgtt 1021 ttcggctcct taccgtggca aaaaaatcat tataagctcc cctccagatt ccacaaaagc 1081 ttgcaagggc gatcgccact ggtcttcgtc aggaaatgtt gatggcgatc ccaagggggt 1141 gacacttgaa aaactgtcta cttttgggga gatgggtgaa atctggtttt aaagtaggat 1201 tgtagccatg atttcggctt gttttttgcg cttttaaatg gaattgcctc tggacatctc 1261 caaaCGAATT GTGAGCGCTC ACAATTCGga attcttaaca aaaaagcagg aataaaatta 1321 acaagatgta aTTgacataa gtcccatcac cgttgtataa atgtgtggaa ttgtgagcgg 1381 ataacaattt cacacaccaa ctcataaagt caagtaggag attaattccA TGGATACCCT 1441 CCTCATTCTC GGCGATAGTC TCTCCGCCGG TTATCGCATG TCTGCCTCTG CCGCCTGGCC 1501 CGCCCTCCTC AATGATAAAT GGCAAAGCAA AACCTCTGTG GTGAATGCCA GTATTTCCGG 1561 CGATACCTCT CAACAAGGCC TCGCCCGTCT CCCCGCCCTC CTCAAACAAC ACCAACCCCG 1621 TTGGGTGTTG GTGGAACTCG GCGGTAATGA TGGCCTCCGC GGTTTTCAAC CCCAACAAAC 1681 CGAACAAACC CTCCGGCAAA TTCTCCAAGA TGTGAAAGCC GCCAATGCCG AACCCCTCCT 1741 CATGCAAATT CGCCTCCCCG CCAATTATGG TCGCCGCTAC AATGAAGCCT TTAGTGCCAT 1801 TTATCCCAAA CTCGCCAAAG AATTTGATGT GCCCCTCCTC CCCTTTTTCA TGGAAGAAGT 1861 GTACCTCAAA CCCCAATGGA TGCAAGATGA TGGTATTCAC CCCAATCGGG ATGCCCAACC 1921 CTTTATTGCC GATTGGATGG CCAAACAACT CCAACCCCTC GTGAATCATG ATAGTTAATC 1981 TAGATTTCTT GTACAAACTC GGCCCTatcc ggctgtctaa caaagcccga aaggaagctg 2041 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg 2101 tcttgacggg ttttttgtct agatcaacgg cctcaaccta ctactgggct gcttcctaat 2161 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaTTTACA 2221 GCTAGCTCAG TCCTAGGTAC AATGCTAGcc ggaggagggt caattcaTgg tggtgaatgt 2281 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc 2341 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc 2401 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc 2461 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc 2521 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg 2581 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg 2641 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac 2701 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt 2761 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca 2821 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg 2881 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactTTag 2941 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc 3001 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg 3061 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg 3121 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt 3181 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt 3241 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc 3301 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 3361 agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc 3421 ccttgagaAA ATGACGAGCG AATTAGTCAT CTTTctAGAT TAacattgca caagataaaa 3481 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta 3541 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt 3601 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct 3661 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca 3721 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga 3781 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg 3841 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg 3901 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca 3961 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg 4021 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc 4081 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata 4141 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg 4201 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat 4261 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt 4321 ttcatttgat gctcgatgag tttttctaag cgatccgaat ggcggaatct tcgggtattg 4381 atgctaccct gaaaatattt tgcagatagg gcccctttag cccactgatt taacaaaacc 4441 gagttacatt tctctaggaa aacattcaca tggacggaaa ccaagtgcct actgttaccc 4501 tcaaacgccc aattaatctg aaggtcatcg tgaccccccg ctggaaagag gaagtacagc 4561 agcaactcca gggacaaata gcgacttttg atggtcaatt acaacaactg gatgtccaag 4621 gaaatcgcac cattagtgaa cttcaacaac agggcaatac cccccaggtc gctcaacaaa 4681 tggaaaatat tcaactccag gttaaccaaa aaaagcggga actccttgag aagaaaaacc 4741 aagtgctcca gcagctccaa caggtacaaa acttagaatt agatcaagag tttctccagg 4801 gtcagatgga gagtttcttt gaagtgaaag tgggcgataa tctggtgcgc aagctgaatg 4861 ttgaacttgt ggtccgggat ggtgttgttg aagaaattcg cggcgacctc taattttttt 4921 gtgattgctg tcgcgtgaca gtgttcggct cccccgatga cacttgcggt gcgggggatt 4981 tttaatgggg aaaaaattca tgaaaattgg cattgtcggg ctgggtttaa tcggtggatc 5041 tttggcgatc gccttccggg aaaaaggctt agaagtgttg ggtatttccc ggaaaaatac 5101 aacctgcgaa acggccttga ggaagggaat tgtcgaccaa gccagcacag atttttctct 5161 tctcaaggat acggatgtgg tgtttctctg cacgccgatc aaagcgattt tgcccacggc 5221 tcaaaccctt attccccacc tcaaatccac ggcgatttGT CATAGCTGTT TCCTGTGTGA 5281 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC 5341 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC 5401 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC 5461 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT 5521 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 5581 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 5641 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 5701 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 5761 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 5821 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 5881 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 5941 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 6001 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 6061 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 6121 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 6181 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 6241 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 6301 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 6361 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 6421 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 6481 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 6541 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 6601 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 6661 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 6721 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 6781 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 6841 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 6901 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 6961 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 7021 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 7081 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 7141 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 7201 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 7261 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 7321 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 7381 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA 7441 TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC //