LOCUS       Exported                9982 bp ds-DNA     circular SYN 10-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 10, 2020 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2935..4428
                     /label=CRY2
                     /note="note=""direct strand 
                     translation""translation=""MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSV
                     FPVFIWCPEEEGQFYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIR
                     VTGATKVVFNHLYDPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTS
                     FNSYWKKCLDMSIESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRA
                     "
     misc_feature    4462..5166
                     /label=mCherry
                     /note="note=""direct strand 
                     translation""translation=""VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFE
                     IEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSF
                     PEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEAS
                     SERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHN
                     "
     misc_feature    5245..6771
                     /label=PLD
                     /note="note=""direct strand 
                     translation""translation=""GGSADSATPHLDAVEQTLRQVSPGLEGDVWER
                     TSGNKLDGSAADPSDWLLQTPGCWGDDKCADRVGTKRLLAKMTENIGNATRTVDISTLA
                     PFPNGAFQDAIVAGLKESAAKGNKLKVRILVGAAPVYHMNVIPSKYRDELTAKLGKAAE
                     NITLNVASMTTSKTAFSWNHSKILVVDGQSALTGGINSWKDDYLDTTHPVSDVDLALTG
                     "
     misc_feature    6772..6837
                     /label=P2A
                     /note="note=""direct strand 
                     translation""translation=""GSGATNFSLLKQAGDVEENPGP"""
     misc_feature    6844..7356
                     /label=CIBN
     misc_feature    7381..7860
                     /label=2xFYVE
ORIGIN
        1 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
       61 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1921 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     1981 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2041 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     2101 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     2161 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     2221 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2281 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2341 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2401 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2461 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2521 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2581 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2641 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2701 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2761 gaggtctata taagcagagc tctccctatc agtgatagag agtcgacgag ctcgtttagt
     2821 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     2881 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacgc caccatgaag
     2941 atggacaaaa agactatagt ttggtttaga agagacctaa ggattgagga taatcctgca
     3001 ttagcagcag ctgctcacga aggatctgtt tttcctgtct tcatttggtg tcctgaagaa
     3061 gaaggacagt tttatcctgg aagagcttca agatggtgga tgaaacaatc acttgctcac
     3121 ttatctcaat ccttgaaggc tcttggatct gacctcactt taatcaaaac ccacaacacg
     3181 atttcagcga tcttggattg tatccgcgtt accggtgcta caaaagtcgt ctttaaccac
     3241 ctctatgatc ctgtttcgtt agttcgggac cataccgtaa aggagaagct ggtggaacgt
     3301 gggatctctg tgcaaagcta caatggagat ctattgtatg aaccgtggga gatatactgc
     3361 gaaaagggca aaccttttac gagtttcaat tcttactgga ggaaatgctt agatatgtcg
     3421 attgaatccg ttatgcttcc tcctccttgg cggttgatgc caataactgc agcggctgaa
     3481 gcgatttggg cgtgttcgat tgaagaacta gggctggaga atgaggccga gaaaccgagc
     3541 aatgcgttgt taactagagc ttggtctcca ggatggagca atgctgataa gttactaaat
     3601 gagttcatcg agaagcagtt gatagattat gcaaagaaca gcaagaaagt tgttgggaat
     3661 tctacttcac tactttctcc gtatctccat ttcggggaaa taagcgtcag acacgttttc
     3721 cagtgtgccc ggatgaaaca aattatatgg gcaagagata agaacagtga aggagaagaa
     3781 agtgcagatc tttttcttag gggaatcggt ttaagagagt attctcggta tatatgtttc
     3841 aacttcccgt ttactcacga gcaatcgttg ttgagtcatc ttcggttttt cccttgggat
     3901 gctgatgttg ataagttcaa ggcctggaga caaggcagga ccggttatcc gttggtggat
     3961 gccggaatga gagagctttg ggctaccgga tggatgcata acagaataag agtgattgtt
     4021 tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat ggggaatgaa gtatttctgg
     4081 gatacacttt tggatgctga tttggaatgt gacatccttg gctggcagta tatctctggg
     4141 agtatccccg atggccacga gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa
     4201 tatgacccag aaggtgagta cataaggcaa tggcttcccg agcttgcgag attgccaact
     4261 gaatggatcc atcatccatg ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa
     4321 ctcggaacaa actatgcgaa acccattgta gacatcgaca cagctcgtga gctactagct
     4381 aaagctattt caagaacccg tgaagcacag atcatgatcg gagcagcagg tggaggcggt
     4441 tcaggcggag gtggcagcgg cgtgagcaag ggcgaggagg ataacatggc catcatcaag
     4501 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     4561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     4681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     4741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     4801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     4861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     4921 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     4981 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     5041 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     5101 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     5161 tacaagagat ctcgaagcgc ggccgcggga gcaggaggag cagctcgagg cgcagcagcg
     5221 ggagcaggag gagcaggtcg atcgggcgga tctgcagatt cagctacacc acatttggac
     5281 gccgttgagc aaacattgag gcaagtgtcc ccaggcctgg agggcgatgt atgggaaaga
     5341 actagtggaa ataaattgga cggaagtgcc gctgatccca gtgattggtt gttgcaaacc
     5401 ccaggatgtt ggggcgatga taaatgcgct gacagagttg ggacaaaaag gttgctggct
     5461 aagatgactg aaaatatagg gaatgccacc agaaccgtag atattagtac acttgcacct
     5521 ttcccaaacg gcgcattcca agacgctatt gtcgctggtt tgaaggagtc cgcagctaaa
     5581 ggcaataagt tgaaagttag gatactggtt ggggctgcac ccgtctacca catgaatgta
     5641 ataccttcca agtataggga cgaattgact gcaaaacttg gcaaagccgc agaaaacata
     5701 actttgaacg tggctagtat gactacatca aaaacagctt tctcctggaa ccatagcaaa
     5761 atcttggttg tagacgggca aagtgccctt actggtggaa taaatagctg gaaggatgat
     5821 tacttggata ctactcatcc agtgtctgat gttgatcttg cactgaccgg accagctgca
     5881 ggctctgccg gtagatacct tgacactttg tggacatgga cctgccaaaa caagagtaac
     5941 atcgcttccg tctggtttgc agcttccggc aacgcagggt gcatgcccac tatgcataaa
     6001 gatacaaacc ccaaggcatc acctgccacc gggaatgtcc cagtaatagc cgttggaggg
     6061 ttgggcgttg gcatcaaaga tgtggacccc aagtccactt tcaggcccga tctgccaaca
     6121 gctagcgata ctaagtgtgt ggtaggactt cacgacaata ctaacgccga tagagactat
     6181 gatacagtca acccagaaga gagtgccttg agagctcttg ttgcttcagc aaaaggtcac
     6241 attgaaatat ctcagcagga tttgaacgct acatgtccac ccttgcctag gtatgacatt
     6301 agattgtacg atgccttggc tgctaaaatg gctgctgggg ttaaggtgag aattgtggtt
     6361 agcgacccag ccaacagggg cgcagtgggc agtgggggtt acagccaaat aaaatcactt
     6421 agtgaaatat cagacacttt gagaaacagg cttgcaaata tcacaggcgg tcaacaagct
     6481 gccaaaactg caatgtgtag caatttgcag cttgcaacct ttagatcctc ccccaacggc
     6541 aaatgggcag acgggcatcc ttatgcccaa caccataagt tggtttccgt cgactcatcc
     6601 acattttata tcggttcaaa aaatttgtac ccatcctggc tgcaagattt tggctacata
     6661 gtggaatccc cagaggctgc caagcaactg gatgccaaat tgcttgaccc tcagtggaag
     6721 tactcacagg agactgcaac agtggattac gcaagaggaa tctgcaacgc tggaagcggc
     6781 gctacaaact ttagtctgct caagcaggct ggcgatgtcg aggaaaaccc aggtcccggt
     6841 accatgaatg gagctatagg aggtgacctt ttgctcaatt ttcctgacat gtcggtccta
     6901 gagcgccaaa gggctcacct caagtacctc aatcccacct ttgattctcc tctcgccggc
     6961 ttctttgccg attcttcaat gattaccggc ggcgagatgg acagctatct ttcgactgcc
     7021 ggtttgaatc ttccgatgat gtacggtgag acgacggtgg aaggtgattc aagactctca
     7081 atttcgccgg aaacgacgct tgggactgga aatttcaagg cagcgaagtt tgatacagag
     7141 actaaggatt gtaatgaggc ggcgaagaag atgacgatga acagagatga cctagtagaa
     7201 gaaggagaag aagagaagtc gaaaataaca gagcaaaaca atgggagcac aaaaagcatc
     7261 aagaagatga aacacaaagc caagaaagaa gagaacaatt tctctaatga ttcatctaaa
     7321 gtgacgaagg aattggagaa aacggattat attcatgtga ccggtggcgc cttagaattc
     7381 gaaagtgatg ccatgttcgc tgctgaaaga gcccctgact gggtggatgc tgaggaatgc
     7441 catcggtgca gagtacagtt tggggtggtg acccgcaagc atcactgccg agcatgtggg
     7501 cagatcttct gtggcaagtg ctcctccaag tactccacca tccccaagtt cggcattgag
     7561 aaggaggtgc gcgtgtgtga gccctgctat gagcagctga acaagaaggc acaaggacaa
     7621 ggatccgaaa gtgatgccat gttcgctgct gaaagagccc ctgactgggt ggatgctgag
     7681 gaatgccatc ggtgcagagt acagtttggg gtggtgaccc gcaagcatca ctgccgagca
     7741 tgtgggcaga tcttctgtgg caagtgctcc tccaagtact ccaccatccc caagttcggc
     7801 attgagaagg aggtgcgcgt gtgtgagccc tgctatgagc agctgaacaa gaaggcatga
     7861 tctagagggc cctattcgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7921 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7981 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8041 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8101 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     8161 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8221 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8281 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8341 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8401 cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct
     8461 tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     8521 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     8581 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     8641 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     8701 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     8761 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     8821 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     8881 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     8941 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     9001 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     9061 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     9121 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     9181 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     9241 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     9301 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     9361 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     9421 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     9481 aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     9541 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     9601 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9661 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9721 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     9781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9961 aatgaatcgg ccaacgcgcg gg
//
