LOCUS       Exported               10695 bp ds-DNA     circular SYN 14-SEP-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 10, 2020 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        844..862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    871..904
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    947..1140
                     /label=hCOL1A1 Nterm
                     /note="color: #993366"
     misc_feature    1063..1128
                     /label=Signal peptide
                     /note="color: #a6acb3"
     CDS             1063..1065
                     /codon_start=1
                     /label=Start site
                     /note="color: #00ff00"
                     /translation="M"
     CDS             1141..1842
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELY"
     CDS             1843..6169
                     /codon_start=1
                     /label=hCOL1A1-Cterm
                     /note="color: #00ff00"
                     /translation="DQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPG
                     PPGPPGLGGNFAPQLSYGYDEKSTGGISVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGE
                     PGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKG
                     HRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGAPGPAGARGND
                     GATGAAGPPGPTGPAGPPGFPGAVGAKGEAGPQGPRGSEGPQGVRGEPGPPGPAGAAGP
                     AGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPGGPPGPKGNSGEPGAPGSKG
                     DTGAKGEPGPVGVQGPPGPAGEEGKRGARGEPGPTGLPGPPGERGGPGSRGFPGADGVA
                     GPKGPAGERGSPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQ
                     DGRPGPPGPPGARGQAGVMGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQG
                     PPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGER
                     GFPGERGVQGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGERGAAGL
                     PGPKGDRGDAGPKGADGSPGKDGVRGLTGPIGPPGPAGAPGDKGESGPSGPAGPTGARG
                     APGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNV
                     GAPGAKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGGKGPRGETGP
                     AGRPGEVGPPGPPGPAGEKGSPGADGPAGAPGTPGPQGIAGQRGVVGLPGQRGERGFPG
                     LPGPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGAK
                     GDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPAGPVGPVGARGPAGPQGP
                     RGDKGETGEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGAPG
                     KDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSFLPQPPQEKAH
                     DGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDW
                     KSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKDKRHVWFGE
                     SMTDGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKA
                     LLLQGSNEIEIRAEGNSRFTYSVTVDGCTSHTGAWGKTVIEYKTTKTSRLRIIDVAPLD
                     VGAPDQEFGFDVGHVCFL*TPSIPTWLPPTQPTFPPTRKQTSNPN*TPSKAKKWETISH
                     GLWKIFFSFAFISQT*FLSLTNRT*PKTKSAFNLTKKKKK"
     misc_feature    6227..6228
                     /label=CTAG in between here
                     /note="color: #a6acb3"
     polyA_signal    6249..6473
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6519..6947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6961..7290
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      7141..7276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             7357..8151
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8325..8446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(8495..8511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8519..8535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8543..8573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8543 .. 8549 / #ffffff / -10
                        2: 8550 .. 8567 / #ffffff
                        3: 8568 .. 8573 / #ffffff / -35"
     protein_bind    8588..8609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8897..9482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9653..10513)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9653 .. 10444 / #ccffcc
                        2: 10445 .. 10513 / #ccffcc / signal sequence
                     Cleavage site after base 10444"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10514..10618)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagaatt
      841 ttgtaatacg actcactata gggcggccgc ataacttcgt atagcataca ttatacgaag
      901 ttatggatca ggccaaatcg gccgagctcg aattcgtcga gagcggagac gggagtttct
      961 cctcggggtc ggagcaggag gcacgcggag tgtgaggcca cgcatgagcg gacgctaacc
     1021 ccctccccag ccacaaagag tctacatgtc tagggtctag acatgttcag ctttgtggac
     1081 ctccggctcc tgctcctctt agcggccacc gccctcctga cgcacggcca agaggatatc
     1141 gtgagcaagg gcgaggagaa taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     1201 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1261 ccctacgagg cctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc
     1321 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggtctacat taagcaccca
     1381 gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg
     1441 atgaacttcg aggacggcgg cattattcac gttaaccagg actcctccct gcaggacggc
     1501 gtgttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1561 cagaagaaga ccatgggctg ggaggcctcc gaggagcgga tgtaccccga ggacggcgcc
     1621 ctgaagagcg agatcaagaa gaggctgaag ctgaaggacg gcggccacta cgccgccgag
     1681 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac
     1741 atcaagttgg acatcgtgtc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1801 gccgagggcc gccactccac cggcggcatg gacgagctgt acgaccaaga aaccaccggc
     1861 gtcgagggac ccaagggaga cactggcccc cgaggcccaa ggggacccgc aggcccccct
     1921 ggccgagatg gcatccctgg acagcctgga cttcccggac cccccggacc ccccggacct
     1981 cccggacccc ctggcctcgg aggaaacttt gctccccagc tgtcttatgg ctatgatgag
     2041 aaatcaaccg gaggaatttc cgtgcctggc cccatgggtc cctctggtcc tcgtggtctc
     2101 cctggccccc ctggtgcacc tggtccccaa ggcttccaag gtccccctgg tgagcctggc
     2161 gagcctggag cttcaggtcc catgggtccc cgaggtcccc caggtccccc tggaaagaat
     2221 ggagatgatg gggaagctgg aaaacctggt cgtcctggtg agcgtgggcc tcctgggcct
     2281 cagggtgctc gaggattgcc cggaacagct ggcctccctg gaatgaaggg acacagaggt
     2341 ttcagtggtt tggatggtgc caagggagat gctggtcctg ctggtcctaa gggtgagcct
     2401 ggcagccctg gtgaaaatgg agctcctggt cagatgggcc cccgtggcct gcctggtgag
     2461 agaggtcgcc ctggagcccc tggccctgct ggtgctcgtg gaaatgatgg tgctactggt
     2521 gctgccgggc cccctggtcc caccggcccc gctggtcctc ctggcttccc tggtgctgtt
     2581 ggtgctaagg gtgaagctgg tccccaaggg ccccgaggct ctgaaggtcc ccagggtgtg
     2641 cgtggtgagc ctggcccccc tggccctgct ggtgctgctg gccctgctgg aaaccctggt
     2701 gctgatggac agcctggtgc taaaggtgcc aatggtgctc ctggtattgc tggtgctcct
     2761 ggcttccctg gtgcccgagg cccctctgga ccccagggcc ccggcggccc tcctggtccc
     2821 aagggtaaca gcggtgaacc tggtgctcct ggcagcaaag gagacactgg tgctaaggga
     2881 gagcctggcc ctgttggtgt tcaaggaccc cctggccctg ctggagagga aggaaagcga
     2941 ggagctcgag gtgaacccgg acccactggc ctgcccggac cccctggcga gcgtggtgga
     3001 cctggtagcc gtggtttccc tggcgcagat ggtgttgctg gtcccaaggg tcccgctggt
     3061 gaacgtggtt ctcctggccc tgctggcccc aaaggatctc ctggtgaagc tggtcgtccc
     3121 ggtgaagctg gtctgcctgg tgccaagggt ctgactggaa gccctggcag ccctggtcct
     3181 gatggcaaaa ctggcccccc tggtcccgcc ggtcaagatg gtcgccccgg acccccaggc
     3241 ccacctggtg cccgtggtca ggctggtgtg atgggattcc ctggacctaa aggtgctgct
     3301 ggagagcccg gcaaggctgg agagcgaggt gttcccggac cccctggcgc tgtcggtcct
     3361 gctggcaaag atggagaggc tggagctcag ggaccccctg gccctgctgg tcccgctggc
     3421 gagagaggtg aacaaggccc tgctggctcc cccggattcc agggtctccc tggtcctgct
     3481 ggtcctccag gtgaagcagg caaacctggt gaacagggtg ttcctggaga ccttggcgcc
     3541 cctggcccct ctggagcaag aggcgagaga ggtttccctg gcgagcgtgg tgtgcaaggt
     3601 ccccctggtc ctgctggtcc ccgaggggcc aacggtgctc ccggcaacga tggtgctaag
     3661 ggtgatgctg gtgcccctgg agctcccggt agccagggcg cccctggcct tcagggaatg
     3721 cctggtgaac gtggtgcagc tggtcttcca gggcctaagg gtgacagagg tgatgctggt
     3781 cccaaaggtg ctgatggctc tcctggcaaa gatggcgtcc gtggtctgac cggccccatt
     3841 ggtcctcctg gccctgctgg tgcccctggt gacaagggtg aaagtggtcc cagcggccct
     3901 gctggtccca ctggagctcg tggtgccccc ggagaccgtg gtgagcctgg tccccccggc
     3961 cctgctggct ttgctggccc ccctggtgct gacggccaac ctggtgctaa aggcgaacct
     4021 ggtgatgctg gtgctaaagg cgatgctggt ccccctggcc ctgccggacc cgctggaccc
     4081 cctggcccca ttggtaatgt tggtgctcct ggagccaaag gtgctcgcgg cagcgctggt
     4141 ccccctggtg ctactggttt ccctggtgct gctggccgag tcggtcctcc tggcccctct
     4201 ggaaatgctg gaccccctgg ccctcctggt cctgctggca aagaaggcgg caaaggtccc
     4261 cgtggtgaga ctggccctgc tggacgtcct ggtgaagttg gtccccctgg tccccctggc
     4321 cctgctggcg agaaaggatc ccctggtgct gatggtcctg ctggtgctcc tggtactccc
     4381 gggcctcaag gtattgctgg acagcgtggt gtggtcggcc tgcctggtca gagaggagag
     4441 agaggcttcc ctggtcttcc tggcccctct ggtgaacctg gcaaacaagg tccctctgga
     4501 gcaagtggtg aacgtggtcc ccctggtccc atgggccccc ctggattggc tggaccccct
     4561 ggtgaatctg gacgtgaggg ggctcctggt gccgaaggtt cccctggacg agacggttct
     4621 cctggcgcca agggtgaccg tggtgagacc ggccccgctg gaccccctgg tgctcctggt
     4681 gctcctggtg cccctggccc cgttggccct gctggcaaga gtggtgatcg tggtgagact
     4741 ggtcctgctg gtcccgccgg tcctgtcggc cctgttggcg cccgtggccc cgccggaccc
     4801 caaggcccac gtggtgacaa gggtgagaca ggcgaacagg gcgacagagg cataaagggt
     4861 caccgtggct tctctggcct ccagggtccc cctggccctc ctggctctcc tggtgaacaa
     4921 ggtccctctg gagcctctgg tcctgctggt ccccgaggtc cccctggctc tgctggtgct
     4981 cctggcaaag atggactcaa cggtctccct ggccccattg ggccccctgg tcctcgcggt
     5041 cgcactggtg atgctggtcc tgttggtccc cccggccctc ctggacctcc tggtccccct
     5101 ggtcctccca gcgctggttt cgacttcagc ttcctgcccc agccacctca agagaaggct
     5161 cacgatggtg gccgctacta ccgggctgat gatgccaatg tggttcgtga ccgtgacctc
     5221 gaggtggaca ccaccctcaa gagcctgagc cagcagatcg agaacatccg gagcccagag
     5281 ggcagccgca agaaccccgc ccgcacctgc cgtgacctca agatgtgcca ctctgactgg
     5341 aagagtggag agtactggat tgaccccaac caaggctgca acctggatgc catcaaagtc
     5401 ttctgcaaca tggagactgg tgagacctgc gtgtacccca ctcagcccag tgtggcccag
     5461 aagaactggt acatcagcaa gaaccccaag gacaagaggc atgtctggtt cggcgagagc
     5521 atgaccgatg gattccagtt cgagtatggc ggccagggct ccgaccctgc cgatgtggcc
     5581 atccagctga ccttcctgcg cctgatgtcc accgaggcct cccagaacat cacctaccac
     5641 tgcaagaaca gcgtggccta catggaccag cagactggca acctcaagaa ggccctgctc
     5701 ctccagggct ccaacgagat cgagatccgc gccgagggca acagccgctt cacctacagc
     5761 gtcactgtcg atggctgcac gagtcacacc ggagcctggg gcaagacagt gattgaatac
     5821 aaaaccacca agacctcccg cctgcgcatc atcgatgtgg cccccttgga cgttggtgcc
     5881 ccagaccagg aattcggctt cgacgttggc catgtctgct tcctgtaaac tccctccatc
     5941 ccaacctggc tccctcccac ccaaccaact ttccccccaa cccggaaaca gacaagcaac
     6001 ccaaactgaa ccccctcaaa agccaaaaaa tgggagacaa tttcacatgg actttggaaa
     6061 atattttttt cctttgcatt catctctcaa acttagtttt tatctttgac caaccgaaca
     6121 tgaccaaaaa ccaaaagtgc attcaacctt accaaaaaaa aaaaaaaaac tctccagcgc
     6181 tggatcccct ggtgctgatg gtcctgctgg tgctcctggt actccccaaa cccgctgatc
     6241 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6301 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6361 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6421 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6481 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6961 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7021 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7081 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7141 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7201 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7261 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7321 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     7381 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7441 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     7501 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     7561 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     7621 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     7681 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     7741 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     7801 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     7861 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     7921 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     7981 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     8041 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     8101 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     8161 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     8221 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8281 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     8341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8461 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8521 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8581 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8641 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8701 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8761 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8821 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9301 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9421 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9481 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9541 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9601 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9661 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9721 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9781 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9841 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9901 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9961 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10021 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10081 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10141 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10201 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10261 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10321 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10381 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10441 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10501 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10561 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10621 catttccccg aaaagtgcca cctgacgtct aaacaaatag gggttccgcg cacatttccc
    10681 cgaaaagtgc cacct
//
