LOCUS pAAV_EF1a_DIO_hC 5944 bp ds-DNA circular 24-FEB-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin 5219..5901 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1594..1595,1597..1598,1600..1604,1606..1610, 1612..1613,1615..1616,1618..1619,1621..1622,1624..1625, 1627..1631,1633..1634,1636..1640,1642..1646,1648..1652, 1654..1658,1660..1661,1663..1664,1666..1673,1675..1679, 1681..1682,1684..1688,1690..1691,1693..1706,1708..1712, 1714..1715,1717..1721,1723..1727,1729..1730,1732..1733, 1735..1736,1738..1739,1741..1745,1747..1754,1756..1760, 1762..1766,1768..1772,1774..1775,1777..1778,1780..1784, 1786..1790,1792..1799,1801..1805,1807..1808,1810..1814, 1816..1826,1828..1829,1831..1832,1834..1841,1843..1844, 1846..1847,1849..1853,1855..1859,1861..1862,1864..1868, 1870..1874,1876..1877,1879..1880,1882..1883,1885..1886, 1888..1895,1897..1901,1903..1904,1906..1910,1912..1916, 1918..1928,1930..1937,1939..1940,1942..1943,1945..1946, 1948..1949,1951..1952,1954..1961,1963..1967,1969..1970, 1972..1976,1978..1982,1984..1985,1987..1994,1996..1997, 1999..2000,2002..2003,2005..2006,2008..2009,2011..2015, 2017..2024,2026..2027,2029..2033,2035..2036,2038..2039, 2041..2042) /label=Vivid /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=#ff80c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 3439..3745 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2044..2046 /label=VP16 Transactivation Domain /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 3422..3877 /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1456..1578 /label=N-Cre /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1453..1455 /label=iCRE /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 4462..5121 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal 2690..3170 /label=hGH polyA signal /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 89..104 /label=REP78 Binding Element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3319..3334 /label=REP78 Binding Element(1) /ApEinfo_label=REP78 Binding Element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(14..29) /label=REP78 Binding Element(2) /ApEinfo_label=REP78 Binding Element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3244..3259) /label=REP78 Binding Element(3) /ApEinfo_label=REP78 Binding Element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 121..141 /label=D' Element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3207..3227) /label=D' Element(1) /ApEinfo_label=D' Element /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 60..68 /label=ITR HAIRPIN REPEAT 1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3268..3276 /label=ITR HAIRPIN REPEAT 1(1) /ApEinfo_label=ITR HAIRPIN REPEAT 1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(72..80) /label=ITR HAIRPIN REPEAT 1(2) /ApEinfo_label=ITR HAIRPIN REPEAT 1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3280..3288) /label=ITR HAIRPIN REPEAT 1(3) /ApEinfo_label=ITR HAIRPIN REPEAT 1 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 38..46 /label=ITR HAIRPIN REPEAT 2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3290..3298 /label=ITR HAIRPIN REPEAT 2(1) /ApEinfo_label=ITR HAIRPIN REPEAT 2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(50..58) /label=ITR HAIRPIN REPEAT 2(2) /ApEinfo_label=ITR HAIRPIN REPEAT 2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3302..3310) /label=ITR HAIRPIN REPEAT 2(3) /ApEinfo_label=ITR HAIRPIN REPEAT 2 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 enhancer 2070..2659 /label=WRE /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=#cc66ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 242..1419 /label=Human EF1A 1.2 kb Promoter /ApEinfo_fwdcolor=#ccff66 /ApEinfo_revcolor=#ccff66 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(4264..5124) /label=Beta Lactamase AMP Resistance /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc 1441 gacgccgcca ccATGACGAG TGATGAGGTT CGCAAGAACC TGATGGACAT GTTCAGGGAT 1501 CGCCAGGCGT TTTCTGAGCA TACCTGGAAA ATGCTTCTGT CCGTTTGCCG GTCGTGGGCG 1561 GCATGGTGCA AGTTGAATGG AGGCGGTGGA GGTCAtACaC TGTAtGCCCC tGGaGGaTAt 1621 GAtATtATGG GaTAtCTGAT tCAGATtATG AAaAGACCtA AtCCtCAGGT GGAaCTGGGa 1681 CCtGTGGAtA CaAGCGTGGC CCTGATtCTG TGtGAtCTGA AaCAGAAaGA tACaCCtATt 1741 GTGTAtGCCA GCGAaGCCTT tCTGTAtATG ACaGGaTAtA GCAAtGCCGA aGTGCTGGGa 1801 AGAAAtTGtA GATTtCTGCA GAGCCCtGAt GGaATGGTGA AaCCtAAaAG CACaAGAAAa 1861 TAtGTGGAtA GCAAtACaAT tAAtACaATG AGAAAaGCCA TtGAtAGAAA tGCCGAaGTG 1921 CAGGTGGAaG TGGTGAAtTT tAAaAAaAAt GGaCAGAGAT TtGTGAAtTT tCTGACaATG 1981 ATtCCtGTGA GAGAtGAaAC aGGaGAaTAt AGATAtAGCA TGGGaTTtCA GTGtGAaACa 2041 GAaTAAgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa 2101 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 2161 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 2221 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 2281 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 2341 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 2401 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 2461 gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg 2521 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 2581 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 2641 ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc 2701 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca 2761 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata 2821 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg 2881 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa 2941 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc 3001 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca 3061 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca 3121 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg 3181 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc 3241 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 3301 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg 3361 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag 3421 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 3481 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 3541 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 3601 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg 3661 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 3721 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta 3781 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 3841 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc 3901 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 3961 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 4021 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 4081 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 4141 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 4201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 4261 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 4321 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 4381 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 4441 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 4501 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 4561 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 4621 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 4681 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 4741 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 4801 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 4861 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 4921 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 4981 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 5041 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 5101 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 5161 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5401 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 5461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 5521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 5581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 5641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 5701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 5761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 5821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 5881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 5941 atgt //