LOCUS       pAAV_EF1a_DIO_hC        5944 bp ds-DNA     circular     24-FEB-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      5219..5901
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1594..1595,1597..1598,1600..1604,1606..1610,
                     1612..1613,1615..1616,1618..1619,1621..1622,1624..1625,
                     1627..1631,1633..1634,1636..1640,1642..1646,1648..1652,
                     1654..1658,1660..1661,1663..1664,1666..1673,1675..1679,
                     1681..1682,1684..1688,1690..1691,1693..1706,1708..1712,
                     1714..1715,1717..1721,1723..1727,1729..1730,1732..1733,
                     1735..1736,1738..1739,1741..1745,1747..1754,1756..1760,
                     1762..1766,1768..1772,1774..1775,1777..1778,1780..1784,
                     1786..1790,1792..1799,1801..1805,1807..1808,1810..1814,
                     1816..1826,1828..1829,1831..1832,1834..1841,1843..1844,
                     1846..1847,1849..1853,1855..1859,1861..1862,1864..1868,
                     1870..1874,1876..1877,1879..1880,1882..1883,1885..1886,
                     1888..1895,1897..1901,1903..1904,1906..1910,1912..1916,
                     1918..1928,1930..1937,1939..1940,1942..1943,1945..1946,
                     1948..1949,1951..1952,1954..1961,1963..1967,1969..1970,
                     1972..1976,1978..1982,1984..1985,1987..1994,1996..1997,
                     1999..2000,2002..2003,2005..2006,2008..2009,2011..2015,
                     2017..2024,2026..2027,2029..2033,2035..2036,2038..2039,
                     2041..2042)
                     /label=Vivid
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=#ff80c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3439..3745
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2044..2046
                     /label=VP16 Transactivation Domain
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3422..3877
                     /label=M13 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1456..1578
                     /label=N-Cre
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1453..1455
                     /label=iCRE
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4462..5121
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    2690..3170
                     /label=hGH polyA signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    89..104
                     /label=REP78 Binding Element
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3319..3334
                     /label=REP78 Binding Element(1)
                     /ApEinfo_label=REP78 Binding Element
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(14..29)
                     /label=REP78 Binding Element(2)
                     /ApEinfo_label=REP78 Binding Element
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3244..3259)
                     /label=REP78 Binding Element(3)
                     /ApEinfo_label=REP78 Binding Element
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    121..141
                     /label=D' Element
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3207..3227)
                     /label=D' Element(1)
                     /ApEinfo_label=D' Element
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    60..68
                     /label=ITR HAIRPIN REPEAT 1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3268..3276
                     /label=ITR HAIRPIN REPEAT 1(1)
                     /ApEinfo_label=ITR HAIRPIN REPEAT 1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(72..80)
                     /label=ITR HAIRPIN REPEAT 1(2)
                     /ApEinfo_label=ITR HAIRPIN REPEAT 1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3280..3288)
                     /label=ITR HAIRPIN REPEAT 1(3)
                     /ApEinfo_label=ITR HAIRPIN REPEAT 1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    38..46
                     /label=ITR HAIRPIN REPEAT 2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3290..3298
                     /label=ITR HAIRPIN REPEAT 2(1)
                     /ApEinfo_label=ITR HAIRPIN REPEAT 2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(50..58)
                     /label=ITR HAIRPIN REPEAT 2(2)
                     /ApEinfo_label=ITR HAIRPIN REPEAT 2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3302..3310)
                     /label=ITR HAIRPIN REPEAT 2(3)
                     /ApEinfo_label=ITR HAIRPIN REPEAT 2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     enhancer        2070..2659
                     /label=WRE
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#cc66ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        242..1419
                     /label=Human EF1A 1.2 kb Promoter
                     /ApEinfo_fwdcolor=#ccff66
                     /ApEinfo_revcolor=#ccff66
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4264..5124)
                     /label=Beta Lactamase AMP Resistance
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gacgccgcca ccATGACGAG TGATGAGGTT CGCAAGAACC TGATGGACAT GTTCAGGGAT
     1501 CGCCAGGCGT TTTCTGAGCA TACCTGGAAA ATGCTTCTGT CCGTTTGCCG GTCGTGGGCG
     1561 GCATGGTGCA AGTTGAATGG AGGCGGTGGA GGTCAtACaC TGTAtGCCCC tGGaGGaTAt
     1621 GAtATtATGG GaTAtCTGAT tCAGATtATG AAaAGACCtA AtCCtCAGGT GGAaCTGGGa
     1681 CCtGTGGAtA CaAGCGTGGC CCTGATtCTG TGtGAtCTGA AaCAGAAaGA tACaCCtATt
     1741 GTGTAtGCCA GCGAaGCCTT tCTGTAtATG ACaGGaTAtA GCAAtGCCGA aGTGCTGGGa
     1801 AGAAAtTGtA GATTtCTGCA GAGCCCtGAt GGaATGGTGA AaCCtAAaAG CACaAGAAAa
     1861 TAtGTGGAtA GCAAtACaAT tAAtACaATG AGAAAaGCCA TtGAtAGAAA tGCCGAaGTG
     1921 CAGGTGGAaG TGGTGAAtTT tAAaAAaAAt GGaCAGAGAT TtGTGAAtTT tCTGACaATG
     1981 ATtCCtGTGA GAGAtGAaAC aGGaGAaTAt AGATAtAGCA TGGGaTTtCA GTGtGAaACa
     2041 GAaTAAgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     2101 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2161 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2221 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2281 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2341 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2401 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2461 gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg
     2521 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2581 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     2641 ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc
     2701 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     2761 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     2821 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     2881 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     2941 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     3001 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     3061 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     3121 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg
     3181 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     3241 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     3301 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     3361 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     3421 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3481 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3541 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3601 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     3661 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3721 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     3781 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3841 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     3901 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3961 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4021 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     4081 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4141 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4261 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4321 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4381 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4441 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4501 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4561 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4621 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4681 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4741 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4801 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4861 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4921 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4981 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5041 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5101 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5161 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5401 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     5461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5941 atgt     
//
