LOCUS pAAV_EF1a_iFlpV 7669 bp ds-DNA linear 30-OCT-2019 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin 6944..7626 /label=ColE1 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1993..2003 /label=New Feature /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 5164..5470 /label=F1 ori /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1441..1521 /locus_tag="Flpo" /label=misc_feature /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 5147..5602 /label=M13 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2081..2088 /label=New Feature(1) /ApEinfo_label="New Feature" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 6187..6846 /label=AmpR /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2125..2574 /label=VIVID Codon diversified /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="#ff0080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 4415..4895 /label=hGH polyA signal /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2095..2118 /label=NLS /ApEinfo_fwdcolor="#ffad48" /ApEinfo_revcolor="#ffad48" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 89..104 /label=REP78 Binding Element /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1528..1977 /label=VVD-N /ApEinfo_fwdcolor="#ffe33c" /ApEinfo_revcolor="#ffe33c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5044..5059 /label=REP78 Binding Element(1) /ApEinfo_label="REP78 Binding Element" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(14..29) /label=REP78 Binding Element(2) /ApEinfo_label="REP78 Binding Element" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(4969..4984) /label=REP78 Binding Element(3) /ApEinfo_label="REP78 Binding Element" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 121..141 /label=D' Element /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(4932..4952) /label=D' Element(1) /ApEinfo_label="D' Element" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 60..68 /label=ITR HAIRPIN REPEAT 1 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4993..5001 /label=ITR HAIRPIN REPEAT 1(1) /ApEinfo_label="ITR HAIRPIN REPEAT 1" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(72..80) /label=ITR HAIRPIN REPEAT 1(2) /ApEinfo_label="ITR HAIRPIN REPEAT 1" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(5005..5013) /label=ITR HAIRPIN REPEAT 1(3) /ApEinfo_label="ITR HAIRPIN REPEAT 1" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 38..46 /label=ITR HAIRPIN REPEAT 2 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5015..5023 /label=ITR HAIRPIN REPEAT 2(1) /ApEinfo_label="ITR HAIRPIN REPEAT 2" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(50..58) /label=ITR HAIRPIN REPEAT 2(2) /ApEinfo_label="ITR HAIRPIN REPEAT 2" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(5027..5035) /label=ITR HAIRPIN REPEAT 2(3) /ApEinfo_label="ITR HAIRPIN REPEAT 2" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" enhancer 3795..4384 /label=WRE /ApEinfo_fwdcolor="#cc66ff" /ApEinfo_revcolor="#cc66ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 242..1419 /label=Human EF1A 1.2 kb Promoter /ApEinfo_fwdcolor="#ccff66" /ApEinfo_revcolor="#ccff66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(5989..6849) /label=Beta Lactamase AMP Resistance /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2581..3771 /label=Flpo /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2023..2043 /label=New Feature(2) /ApEinfo_label="New Feature" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2125..2574 /label=VIVID Codon diversified(1) /ApEinfo_label="VIVID Codon diversified" /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="#ff0080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1993..2016 /label=NLS(1) /ApEinfo_label="NLS" /ApEinfo_fwdcolor="#ffad48" /ApEinfo_revcolor="#ffad48" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2023..2088 /label=P2A /ApEinfo_fwdcolor="#6edbff" /ApEinfo_revcolor="#6edbff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1528..1977 /label=N-VVD /ApEinfo_fwdcolor="#a7ff3a" /ApEinfo_revcolor="#a7ff3a" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc tgccgccacc 1441 atgagccagt tcgacatcct gtgcaagacc ccccccaagg tgctggtgcg gcagttcgtg 1501 gagagattcg agaggcccag cGGTACCCAt ACaCTGTAtG CCCCtGGaGG aTAtGAtATt 1561 ATGGGaTAtC TGATtCAGAT tATGAAaAGA CCtAAtCCtC AGGTGGAaCT GGGaCCtGTG 1621 GAtACaAGCG TGGCCCTGAT tCTGTGtGAt CTGAAaCAGA AaGAtACaCC tATtGTGTAt 1681 GCCAGCGAaG CCTTtCTGTA tATGACaGGa TAtAGCAAtG CCGAaGTGCT GGGaAGAAAt 1741 TGtAGATTtC TGCAGAGCCC tGAtGGaATG GTGAAaCCtA AaAGCACaAG AAAaTAtGTG 1801 GAtAGCAAtA CaATtAAtAC aATGAGAAAa GCCATtGAtA GAAAtGCCGA aGTGCAGGTG 1861 GAaGTGGTGA AtTTtAAaAA aAAtGGaCAG AGATTtGTGA AtTTtCTGAC aATGATtCCt 1921 GTGAGAGAtG AaACaGGaGA aTAtAGATAt AGCATGGGaT TtCAGTGtGA aACaGAaGGC 1981 GGAAGCGGTG GCGTGCCCAA GAAGAAGAGG AAAGTCGGAT CCGGCAGCGG CGCCACCAAC 2041 TTCAGCCTGC TGAAGCAGGC CGGCGACGTG GAGGAGAACC CCGGCCCCCT CGAGGTGCCC 2101 AAGAAGAAGA GGAAAGTCGG CGGACATACT CTGTACGCTC CCGGAGGGTA TGACATAATG 2161 GGTTACCTAA TCCAGATTAT GAAGCGTCCT AATCCCCAGG TTGAGTTGGG ACCAGTGGAC 2221 ACCAGCGTAG CCTTAATTCT TTGCGACCTT AAACAGAAAG ATACACCAAT AGTATACGCG 2281 AGTGAGGCTT TTTTGTATAT GACGGGTTAT TCCAACGCGG AAGTGTTAGG ACGAAATTGT 2341 CGGTTTCTAC AATCACCGGA TGGGATGGTC AAACCGAAAA GTACTAGAAA GTATGTGGAT 2401 TCGAATACAA TTAACACCAT GAGAAAGGCA ATCGATAGGA ACGCAGAAGT ACAAGTCGAG 2461 GTTGTCAATT TCAAAAAGAA TGGGCAACGC TTCGTAAACT TTCTCACTAT GATACCTGTT 2521 AGGGACGAAA CGGGCGAGTA TCGATACTCT ATGGGCTTCC AATGTGAAAC AGAAGGTACC 2581 ggcgagaaga tcgccagctg tgccgccgag ctgacctacc tgtgctggat gatcacccac 2641 aacggcaccg ccatcaagag ggccaccttc atgagctaca acaccatcat cagcaacagc 2701 ctgagcttcg acatcgtgaa caagagcctg cagttcaagt acaagaccca gaaggccacc 2761 atcctggagg ccagcctgaa gaagctgatc cccgcctggg agttcaccat catcccttac 2821 aacggccaga agcaccagag cgacatcacc gacatcgtgt ccagcctgca gctgcagttc 2881 gagagcagcg aggaggccga caagggcaac agccacagca agaagatgct gaaggccctg 2941 ctgtccgagg gcgagagcat ctgggagatc accgagaaga tcctgaacag cttcgagtac 3001 accagcaggt tcaccaagac caagaccctg taccagttcc tgttcctggc cacattcatc 3061 aactgcggca ggttcagcga catcaagaac gtggacccca agagcttcaa gctggtgcag 3121 aacaagtacc tgggcgtgat cattcagtgc ctggtgaccg agaccaagac aagcgtgtcc 3181 aggcacatct actttttcag cgccagaggc aggatcgacc ccctggtgta cctggacgag 3241 ttcctgagga acagcgagcc cgtgctgaag agagtgaaca ggaccggcaa cagcagcagc 3301 aacaagcagg agtaccagct gctgaaggac aacctggtgc gcagctacaa caaggccctg 3361 aagaagaacg ccccctaccc catcttcgct atcaagaacg gccctaagag ccacatcggc 3421 aggcacctga tgaccagctt tctgagcatg aagggcctga ccgagctgac aaacgtggtg 3481 ggcaactgga gcgacaagag ggcctccgcc gtggccagga ccacctacac ccaccagatc 3541 accgccatcc ccgaccacta cttcgccctg gtgtccaggt actacgccta cgaccccatc 3601 agcaaggaga tgatcgccct gaaggacgag accaacccca tcgaggagtg gcagcacatc 3661 gagcagctga agggcagcgc cgagggcagc atcagatacc ccgcctggaa cggcatcatc 3721 agccaggagg tgctggacta cctgagcagc tacatcaaca ggcggatctg agaattcgat 3781 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt 3841 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 3901 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 3961 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 4021 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 4081 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 4141 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc 4201 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac 4261 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 4321 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 4381 ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc 4441 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa 4501 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg 4561 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt 4621 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg 4681 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca 4741 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg 4801 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac 4861 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac 4921 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc 4981 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc 5041 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac 5101 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc 5161 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 5221 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 5281 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 5341 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag 5401 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 5461 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg 5521 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 5581 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca 5641 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 5701 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 5761 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta 5821 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 5881 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 5941 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 6001 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 6061 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 6121 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 6181 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 6241 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 6301 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 6361 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 6421 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 6481 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 6541 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 6601 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 6661 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 6721 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 6781 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 6841 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 6901 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 6961 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 7021 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 7081 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 7141 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 7201 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 7261 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 7321 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 7381 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 7441 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 7501 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 7561 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 7621 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt //