LOCUS       pYFP-lacY               6242 bp ds-DNA     circular     11-FEB-2020
DEFINITION  .
ACCESSION   urn.local...y-bjttt86
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /locus_tag="L3S3P21 Terminator"
                     /note="Geneious type: terminator"
                     /standard_name="L3S3P21 Terminator"
     misc_feature    73..128
                     /locus_tag="PTac Promoter"
                     /note="Geneious type: promoter"
                     /standard_name="Ptac"
     misc_feature    212..223
                     /locus_tag="B0064 RBS"
                     /note="Geneious type: RBS"
                     /standard_name="RBS"
     misc_feature    230..949
                     /locus_tag="YFP (3SNP)"
                     /note="Geneious type: CDS"
                     /standard_name="YFP"
     RBS             988..994
     CDS             1001..2254
                     /gene="lacY"
                     /locus_tag="b0343"
                     /gene_synonym="ECK0340; JW0334"
                     /function="transport; Transport of small molecules:
                     Carbohydrates, organic acids, alcohols"
                     /note="galactoside permease (lactose permease, M protein)
                     (MFS family)"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="NP_414877.1"
                     /db_xref="GI:16128328"
                     /db_xref="ASAP:ABE-0001181"
                     /db_xref="UniProtKB/Swiss-Prot:P02920"
                     /db_xref="EcoGene:EG10526"
                     /db_xref="GeneID:949083"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     misc_feature    2255..2315
                     /locus_tag="L3S2P21 Terminator"
                     /note="Geneious type: terminator"
                     /standard_name="L3S2P21 terminator"
     misc_feature    2320..2373
                     /locus_tag="PLacI (up until -1)"
                     /note="Geneious type: promoter"
                     /standard_name="PlacI"
     misc_feature    2402..3484
                     /locus_tag="LacI"
                     /note="Geneious type: CDS"
                     /standard_name="lacI"
     misc_feature    3503..4126
                     /locus_tag="TetR"
                     /note="Geneious type: CDS"
                     /standard_name="tetR"
     misc_feature    4130..4246
                     /locus_tag="Native AraC Terminator (2SNP)"
                     /note="Geneious type: terminator"
                     /standard_name="Native araC terminator"
     misc_feature    4419..5255
                     /locus_tag="p15A Origin of Replication"
                     /note="Geneious type: rep_origin"
                     /standard_name="p15A origin of replication"
     misc_feature    4790
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    complement(5327..6142)
                     /locus_tag="KanR"
                     /note="Geneious type: CDS"
                     /standard_name="Kanamycin Kinase"
     misc_feature    6110
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
ORIGIN      
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgtgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgc
      121 tcacaattag ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag
      181 cctctacaaa taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa
      241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      361 cctgaagttc atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      421 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt
      481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      661 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      721 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      781 gcagaacacc ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta
      841 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat aataaccggg
      961 caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac
     1021 aaacttttgg atgttcggtt tattcttttt cttttacttt tttatcatgg gagcctactt
     1081 cccgtttttc ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat
     1141 tatttttgcc gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc
     1201 tgacaaactc gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt
     1261 tgcgccgttc tttattttta tcttcgggcc actgttacaa tacaacattt tagtaggatc
     1321 gattgttggt ggtatttatc taggcttttg ttttaacgcc ggtgcgccag cagtagaggc
     1381 atttattgag aaagtcagcc gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg
     1441 ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc atgttcacca tcaataatca
     1501 gtttgttttc tggctgggct ctggctgtgc actcatcctc gccgttttac tctttttcgc
     1561 caaaacggat gcgccctctt ctgccacggt tgccaatgcg gtaggtgcca accattcggc
     1621 atttagcctt aagctggcac tggaactgtt cagacagcca aaactgtggt ttttgtcact
     1681 gtatgttatt ggcgtttcct gcacctacga tgtttttgac caacagtttg ctaatttctt
     1741 tacttcgttc tttgctaccg gtgaacaggg tacgcgggta tttggctacg taacgacaat
     1801 gggcgaatta cttaacgcct cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg
     1861 tgggaaaaac gccctgctgc tggctggcac tattatgtct gtacgtatta ttggctcatc
     1921 gttcgccacc tcagcgctgg aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc
     1981 gttcctgctg gtgggctgct ttaaatatat taccagccag tttgaagtgc gtttttcagc
     2041 gacgatttat ctggtctgtt tctgcttctt taagcaactg gcgatgattt ttatgtctgt
     2101 actggcgggc aatatgtatg aaagcatcgg tttccagggc gcttatctgg tgctgggtct
     2161 ggtggcgctg ggcttcacct taatttccgt gttcacgctt agcggccccg gcccgctttc
     2221 cctgctgcgt cgtcaggtga atgaagtcgc ttaactcggt accaaattcc agaaaagagg
     2281 cctcccgaaa ggggggcctt ttttcgtttt ggtccaatgg cggcgcgcca tcgaatggcg
     2341 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     2401 tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     2461 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     2521 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     2581 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     2641 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     2701 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     2761 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     2821 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     2881 tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     2941 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     3001 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     3061 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     3121 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     3181 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagatagctc
     3241 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     3301 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     3361 agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     3421 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3481 gtgataatcc aggaggaaaa aaatgtccag attagataaa agtaaagtga ttaacagcgc
     3541 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
     3601 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
     3661 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
     3721 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
     3781 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
     3841 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
     3901 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
     3961 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
     4021 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
     4081 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcctaataat tggtaacgaa
     4141 tcagacaatt gacggctcga gggagtagca tagggtttgc agaatccctg cttcgtccat
     4201 ttgacaggca cattatgcat cgatgataag ctgtcaaaca tgagcagatc ctctacgccg
     4261 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     4321 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcaaa tattttatct
     4381 gaggtgcttc ctcgctcact gactcgctgc acgaggcaga cctcagcgct agcggagtgt
     4441 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     4501 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     4561 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     4621 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     4681 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     4741 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     4801 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     4861 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     4921 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     4981 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     5041 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     5101 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     5161 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     5221 agaccaaaac gatctcaaga agatcatctt attaaggggt ctgacgctca gtggaacgaa
     5281 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccttag aaaaactcat
     5341 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     5401 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     5461 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     5521 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     5581 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     5641 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     5701 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     5761 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     5821 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     5881 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     5941 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     6001 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     6061 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg
     6121 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     6181 ttattgttca tgatgatata tttttatctt gtgcaatgta catcagagat tttgagacac
     6241 aa
//
