LOCUS 445_pCEFL_GAl4dbd_KLF4_1-329_with_old_GAL4.txt.xdna 7487 bp DNA circular 2/12/2020 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers promoter 218..1484 /label=EF-1a /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=vntifkey="29" - label=EF-1a - 218..1484 \ misc_feature 1507..1519 /label=SfiI A /SerialCloner_Color=&h80FF00 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1548..1988 /label=Gal4 DNA-binding domain /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 2003..2995 /label=KLF4 1-329 /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from pCEFL GAl4dbd KLF4 1-329.txt.xdna [993 nt] : (BamHI[1988] / NotI[2984]) \ CDS 4192..4986 /label=Neomycin\(G418\resistance) /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=vntifkey="4" - label=Neomycin(G418resistance) - 2766..3560 \ polyA_signal 3083..3307 /label=BGH\pA\signal /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=vntifkey="25" - label=BGHpAsignal - 1657..1881 \ primer_bind complement(5326..5346) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(3040..3057) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(5714..6342) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(5352..5374) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(6494..7153) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 4195..4986 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(7393..7421) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(5379..5408) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3976..4053 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 3080..3307 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3011..3023 /label=SfiI B /SerialCloner_Color=&h80FF80 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True ORIGIN 1 gacggatcgg gagatcgatc tcccgatccc ctatggtcga ctctcagtac aatctgctct 61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag 121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat 181 ctgcttaggg ttaggcgttt tgcgctgctt cgagcttgca aagatggata aagttttaaa 241 cagagaggaa tctttgcagc taatggacct tgtaggtctt gaaaggagtg ggaattggct 301 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 361 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 421 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 481 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 541 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 601 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 661 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 721 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 781 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 841 caagatagtc ttgtaaatgc gggccaagat cgatctgcac actggtattt cggtttttgg 901 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct 961 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt 1021 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc 1081 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg 1141 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt 1201 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct 1261 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc 1321 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt gggcacttga 1381 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc 1441 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagaattg gcgaagctca 1501 gttaacggcc attgtggcca tcaagctTGA AGCAAGCCTC CTGAAAGATG AAGCTACTGT 1561 CTTCTATCGA ACAAGCATGC GATATTTGCC GACTTAAAAA GCTCAAGTGC TCCAAAGAAA 1621 AACCGAAGTG CGCCAAGTGT CTGAAGAACA ACTGGGAGTG TCGCTACTCT CCCAAAACCA 1681 AAAGGTCTCC GCTGACTAGG GCACATCTGA CAGAAGTGGA ATCAAGGCTA GAAAGACTGG 1741 AACAGCTATT TCTACTGATT TTTCCTCGAG AAGACCTTGA CATGATTTTG AAAATGGATT 1801 CTTTACAGGA TATAAAAGCA TTGTTAACAG GATTATTTGT ACAAGATAAT GTGAATAAAG 1861 ATGCCGTCAC AGATAGATTG GCTTCAGTGG AGACTGATAT GCCTCTAACA TTGAGACAGC 1921 ATAGAATAAG TGCGACATCA TCATCGGAAG AGAGTAGTAA CAAAGGTCAA AGACAGTTGA 1981 CTGTATCGCC GGAATTTGga tccAtgaggc agccacctgg cgagtctgac atggctgtca 2041 gcgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga agggagaaga 2101 cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc cacatgaagc 2161 gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc gtggccacag 2221 acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg cccctacctc 2281 ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc tccaattcgc 2341 tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca gcctcctctt 2401 cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc ttcacctatc 2461 cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga ggcctcctct 2521 atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac atcaacgacg 2581 tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac ccggtgtaca 2641 ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc gtgctgaagg 2701 cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc gtcagcaaag 2761 gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg ccgccgcgca 2821 cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc gctggacccc 2881 ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg cagctcccca 2941 gcaggactac cccgaccctg ggtCTTGAGG AAGTGCTGAG CAGCAGGGAC TAAGCggccg 3001 cactcgagat ggccctagcg gcctctagag ggccctattc tatagtgtca cctaaatgct 3061 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 3121 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 3181 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 3241 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 3301 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 3361 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 3421 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 3481 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta 3541 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 3601 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3661 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 3721 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3781 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc 3841 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3901 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3961 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4021 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 4081 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 4141 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 4201 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 4261 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 4321 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 4381 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 4441 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 4501 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 4561 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 4621 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 4681 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 4741 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 4801 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 4861 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 4921 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 4981 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 5041 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 5101 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 5161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 5221 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 5281 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 5341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 5401 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5461 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5521 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 5581 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 5641 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 5701 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 5761 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 5821 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 5881 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 5941 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6001 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6061 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6121 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 6181 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6241 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 6301 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6361 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6421 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6481 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6541 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6601 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6661 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6721 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6781 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 6841 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 6901 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 6961 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7021 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7081 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7141 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7201 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7261 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7321 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7381 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7441 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //