LOCUS       Exported               10224 bp ds-DNA     circular SYN 10-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Glick Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10224
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(385..401)
                     /label=BV13
     CDS             397..974
                     /codon_start=1
                     /label=SEC7
                     /translation="NSIVVKCVLQLLMIELLNELFENEDFAHCIPYKEAIRITRLLEKS
                     YEFSRDFNEDYGLRTRLVEARVVDKIPNLLKQETSAAAVLLDIMFQLYLNDDEKKADLI
                     TRLITICIQVVEGYVSLDDRTMERSINAWRSVIVEILQGYYEFDDEDFRLYCPAMYALV
                     IQILDKSVPTELRHAIKQFLSRVGELYLS"
     primer_bind     975..996
                     /label=yo-mScarlet2i final in-frame QC2.FOR
     misc_feature    976..979
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     CDS             988..1669
                     /codon_start=1
                     /label=yo-mScarlet2-I
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGSG"
     CDS             1675..2356
                     /codon_start=1
                     /label=yo-mScarlet2-I
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGSG"
     CDS             2362..3043
                     /codon_start=1
                     /label=yo-mScarlet2-I
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGSG"
     CDS             3049..3730
                     /codon_start=1
                     /label=yo-mScarlet2-I
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGSG"
     CDS             3736..4417
                     /codon_start=1
                     /label=yo-mScarlet2-I
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGSG"
     CDS             4423..5112
                     /codon_start=1
                     /label=yo-mScarlet2-I
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGSGAS"
     misc_feature    5120..6821
                     /label=3' SEC7
     primer_bind     complement(5220..5240)
                     /label=AE62
     primer_bind     complement(6841..6857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7243..7831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8002..8862)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8863..8967)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(9134..9937)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9938..10154)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctattgttgt caaatgtgtt
      421 ttacagcttt tgatgataga gctgctcaac gaattattcg agaacgaaga ttttgcccac
      481 tgtatccctt acaaggaagc cattagaatt acaagattgt tggagaaatc gtacgaattt
      541 tctcgtgact ttaatgaaga ttatgggtta agaacaagac tagtagaggc tcgtgtagtt
      601 gataaaatac caaacctgtt gaaacaagaa acgagtgctg ctgcagttct ccttgacatt
      661 atgtttcagt tgtatctgaa cgatgatgag aagaaggctg atttaataac tcgtctgatc
      721 accatttgca tccaggtcgt agagggttat gtttctttgg atgatagaac catggaacgt
      781 agtatcaatg cctggcgttc agtgatagtt gaaatattac aaggttacta cgaatttgat
      841 gatgaagact ttagactata ctgtcctgct atgtatgctc tggtgattca aatattagat
      901 aagagcgttc caacggagtt gagacatgct ataaaacaat ttctaagcag agttggtgaa
      961 ttataccttt ctacggatcc aactagtatg gatagtacag aagcagttat aaaggaattc
     1021 atgagattca aagtccacat ggaaggtagt atgaacggtc acgaattcga aatagaaggt
     1081 gaaggtgaag gtagaccata tgaaggtact caaacagcta aattgaaggt tactaaaggt
     1141 ggtccattgc cattttcttg ggatatcttg tctccacaat tcatgtacgg ttcaagagct
     1201 tttattaaac atccagcaga tattccagat tactacaagc aatcatttcc agaaggtttt
     1261 aaatgggaaa gagttatgaa cttcgaagat ggtggtgcag ttactgttac acaagatact
     1321 tctttggaag atggtacatt gatctataag gttaaattga gaggtacaaa ttttccacca
     1381 gatggtccag ttatgcaaaa gaaaactatg ggttgggaag catctacaga aagattgtac
     1441 ccagaagatg gtgttttgaa gggtgacatc aagatggcat tgagattgaa ggatggtggt
     1501 agatatttgg ctgatttcaa gactacatac aaggctaaga aaccagttca aatgccaggt
     1561 gcttacaacg ttgatagaaa attggatatc acttctcata acgaagatta cacagttgtt
     1621 gaacaatacg aaagaagtga aggtagacat agtacaggtg gtagtggtgc tagtatggat
     1681 agtacagaag cagttataaa ggaattcatg agattcaaag tccacatgga aggtagtatg
     1741 aacggtcacg aattcgaaat agaaggtgaa ggtgaaggta gaccatatga aggtactcaa
     1801 acagctaaat tgaaggttac taaaggtggt ccattgccat tttcttggga tatcttgtct
     1861 ccacaattca tgtacggttc aagagctttt attaaacatc cagcagatat tccagattac
     1921 tacaagcaat catttccaga aggttttaaa tgggaaagag ttatgaactt cgaagatggt
     1981 ggtgcagtta ctgttacaca agatacttct ttggaagatg gtacattgat ctataaggtt
     2041 aaattgagag gtacaaattt tccaccagat ggtccagtta tgcaaaagaa aactatgggt
     2101 tgggaagcat ctacagaaag attgtaccca gaagatggtg ttttgaaggg tgacatcaag
     2161 atggcattga gattgaagga tggtggtaga tatttggctg atttcaagac tacatacaag
     2221 gctaagaaac cagttcaaat gccaggtgct tacaacgttg atagaaaatt ggatatcact
     2281 tctcataacg aagattacac agttgttgaa caatacgaaa gaagtgaagg tagacatagt
     2341 acaggtggta gtggtgctag tatggatagt acagaagcag ttataaagga attcatgaga
     2401 ttcaaagtcc acatggaagg tagtatgaac ggtcacgaat tcgaaataga aggtgaaggt
     2461 gaaggtagac catatgaagg tactcaaaca gctaaattga aggttactaa aggtggtcca
     2521 ttgccatttt cttgggatat cttgtctcca caattcatgt acggttcaag agcttttatt
     2581 aaacatccag cagatattcc agattactac aagcaatcat ttccagaagg ttttaaatgg
     2641 gaaagagtta tgaacttcga agatggtggt gcagttactg ttacacaaga tacttctttg
     2701 gaagatggta cattgatcta taaggttaaa ttgagaggta caaattttcc accagatggt
     2761 ccagttatgc aaaagaaaac tatgggttgg gaagcatcta cagaaagatt gtacccagaa
     2821 gatggtgttt tgaagggtga catcaagatg gcattgagat tgaaggatgg tggtagatat
     2881 ttggctgatt tcaagactac atacaaggct aagaaaccag ttcaaatgcc aggtgcttac
     2941 aacgttgata gaaaattgga tatcacttct cataacgaag attacacagt tgttgaacaa
     3001 tacgaaagaa gtgaaggtag acatagtaca ggtggtagtg gtgctagtat ggatagtaca
     3061 gaagcagtta taaaggaatt catgagattc aaagtccaca tggaaggtag tatgaacggt
     3121 cacgaattcg aaatagaagg tgaaggtgaa ggtagaccat atgaaggtac tcaaacagct
     3181 aaattgaagg ttactaaagg tggtccattg ccattttctt gggatatctt gtctccacaa
     3241 ttcatgtacg gttcaagagc ttttattaaa catccagcag atattccaga ttactacaag
     3301 caatcatttc cagaaggttt taaatgggaa agagttatga acttcgaaga tggtggtgca
     3361 gttactgtta cacaagatac ttctttggaa gatggtacat tgatctataa ggttaaattg
     3421 agaggtacaa attttccacc agatggtcca gttatgcaaa agaaaactat gggttgggaa
     3481 gcatctacag aaagattgta cccagaagat ggtgttttga agggtgacat caagatggca
     3541 ttgagattga aggatggtgg tagatatttg gctgatttca agactacata caaggctaag
     3601 aaaccagttc aaatgccagg tgcttacaac gttgatagaa aattggatat cacttctcat
     3661 aacgaagatt acacagttgt tgaacaatac gaaagaagtg aaggtagaca tagtacaggt
     3721 ggtagtggtg ctagtatgga tagtacagaa gcagttataa aggaattcat gagattcaaa
     3781 gtccacatgg aaggtagtat gaacggtcac gaattcgaaa tagaaggtga aggtgaaggt
     3841 agaccatatg aaggtactca aacagctaaa ttgaaggtta ctaaaggtgg tccattgcca
     3901 ttttcttggg atatcttgtc tccacaattc atgtacggtt caagagcttt tattaaacat
     3961 ccagcagata ttccagatta ctacaagcaa tcatttccag aaggttttaa atgggaaaga
     4021 gttatgaact tcgaagatgg tggtgcagtt actgttacac aagatacttc tttggaagat
     4081 ggtacattga tctataaggt taaattgaga ggtacaaatt ttccaccaga tggtccagtt
     4141 atgcaaaaga aaactatggg ttgggaagca tctacagaaa gattgtaccc agaagatggt
     4201 gttttgaagg gtgacatcaa gatggcattg agattgaagg atggtggtag atatttggct
     4261 gatttcaaga ctacatacaa ggctaagaaa ccagttcaaa tgccaggtgc ttacaacgtt
     4321 gatagaaaat tggatatcac ttctcataac gaagattaca cagttgttga acaatacgaa
     4381 agaagtgaag gtagacatag tacaggtggt agtggtgcta gtatggatag tacagaagca
     4441 gttataaagg aattcatgag attcaaagtc cacatggaag gtagtatgaa cggtcacgaa
     4501 ttcgaaatag aaggtgaagg tgaaggtaga ccatatgaag gtactcaaac agctaaattg
     4561 aaggttacta aaggtggtcc attgccattt tcttgggata tcttgtctcc acaattcatg
     4621 tacggttcaa gagcttttat taaacatcca gcagatattc cagattacta caagcaatca
     4681 tttccagaag gttttaaatg ggaaagagtt atgaacttcg aagatggtgg tgcagttact
     4741 gttacacaag atacttcttt ggaagatggt acattgatct ataaggttaa attgagaggt
     4801 acaaattttc caccagatgg tccagttatg caaaagaaaa ctatgggttg ggaagcatct
     4861 acagaaagat tgtacccaga agatggtgtt ttgaagggtg acatcaagat ggcattgaga
     4921 ttgaaggatg gtggtagata tttggctgat ttcaagacta catacaaggc taagaaacca
     4981 gttcaaatgc caggtgctta caacgttgat agaaaattgg atatcacttc tcataacgaa
     5041 gattacacag ttgttgaaca atacgaaaga agtgaaggta gacatagtac aggtggtagt
     5101 ggtgctagct aagcggccgg gctttgttga atggtccagc agattaaaat atgcttagtt
     5161 gtagaacaca gaaaaaatga gaaggttgat atacttttac tcatattaat cctgatttag
     5221 tggcggtgat cctttttaga ttcgtttaat tcataaacaa ggtacatcat atagcaataa
     5281 caaatatgta aactatgtag aaagacttat caagtttagc cattttggtc ctcattcatg
     5341 ggcattttat actaacttca tttttgccga aataagaaag gatgtaatct taacctaaaa
     5401 tatgtaagca attaagggcg cacgtagtca cttgcgaaca aataggaaaa gaaagaaaca
     5461 agcacggata ctgaatgact gagtgtatta ctacacgatt tatctcaacg aatttaagtg
     5521 tggaggacga tcggctgcac gtaggagtca acgttttttt actgtgttca tcacaaaacc
     5581 attaagctga gtcatccata ttgcaaacaa taaggtagca ccgcttccat caaaacacta
     5641 gaacataccg atgttttcac ggatttattg gaaacaaata gcagatgagg gcgtttttac
     5701 ttgtaatgaa ggcgaatgag aaggcaaggc ttacaggctt gttgtttatg ccaagcatca
     5761 atactcgcta tggaggagcg ccatcagcga taacaacaaa gtttgcaagt gcttgcttgg
     5821 atataaaagg cttgacgctt aagataaata gggccatact tgcttgcttt tactattttt
     5881 gttcctcata agcctgattg actacgctat tataaacccc tacattatcc tataatcaac
     5941 ttcacttgtg taacgttgaa tgagttcaac tgagtaatac tcggtcggaa atatacgctc
     6001 catctggcag gaaacatagg ctgcatttat atcttgcaca attatgccct tcgtaggtgt
     6061 atgttttcat gttttccatt atgtcaatgt agatgtatct ttggacaggc aacttttgga
     6121 tcaataaagc acaatttttg agcaatttga gatatccagt atttgtacgg gagtcccaag
     6181 attgtttagc ctaaaaggtt gaggacttgg attattcttc catcaagcgc ctccacccct
     6241 cgcatcctat ttcttttgaa gcatagggga cgagactgtc tgtaatttat ggcgaagtat
     6301 gacaagaaag attcaaactt tgttttacgt atgccccaat aaattctatg gtagcactac
     6361 tagagacgta ttttggtttt gtaataattt tcaaactgct cgctatgatt tgttacaaga
     6421 aacaaacaaa agacacgacc atagggttca tgttggtggt tgcgaagcca catctttaaa
     6481 acattactgc aaattcagca ggagtgagta gcaaaatcga caaaaactag agtacgcaac
     6541 attcccggtc gtaaatttct tatcgtggcg ccagaacatt cccgtccaat gatgagaatg
     6601 tctctaaaac aaatgcagtc acagagattt tctgcgactt ctcgagtgag aatgttgctt
     6661 attggtgcta gtggcttgag atcttcgtca agcaaattgg aatgccccag attctcaaac
     6721 aagcacggca gaaactgaaa tacggccaaa attatgaacc cttttgtggg atctttgtag
     6781 aaataggacc tcaggatagt atgatgaaga atgttggcat gcaagcttgg cgtaatcatg
     6841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7681 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7861 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgagctttt
     9001 tctttccaat tttttttttt tcgtcattat agaaatcatt acgaccgaga ttcccgggta
     9061 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt
     9121 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt
     9181 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa
     9241 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca
     9301 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt
     9361 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct
     9421 tcgcaatgtc aacagtaccc ttagtatatt ctccagtagc tagggagccc ttgcatgaca
     9481 attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttccgcc gcctgcttca
     9541 aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg
     9601 ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt
     9661 ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg
     9721 tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg
     9781 gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac
     9841 acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat
     9901 gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg
     9961 tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacaccac
    10021 atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgctcgg
    10081 agattaccga atcaaaaaaa tttcaaagaa accggaatca aaaaaaagaa caaaaaaaaa
    10141 aaagatgaat tgaaaagctc taagaaacca ttattatcat gacattaacc tataaaaata
    10201 ggcgtatcac gaggcccttt cgtc
//
