LOCUS       Exported               10994 bp ds-DNA     circular SYN 10-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Glick Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2660..2722
                     /codon_start=1
                     /label=sporamin A signal peptide
                     /translation="MKAFTLALFLALSLYLLPNPA"
     CDS             2723..2731
                     /codon_start=1
                     /label=ER export motif
                     /translation="APV"
     CDS             2732..2740
                     /codon_start=1
                     /label=N-linked glycosylation site
                     /translation="NTT"
     CDS             2741..3412
                     /codon_start=1
                     /label=DsRedExpress2
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHKA
                     LKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHHL
                     FQ"
     CDS             3425..3745
                     /codon_start=1
                     /label=FKBP(LV)
                     /translation="GVQVETISPGDGRTFPKRGQTVVVHYTGMLEDGKKLDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGVIPPHATLVFDVELL
                     KLE"
     terminator      3765..4472
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4716..4740
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6040..6669
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7098..8171
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8237..8431
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8775..8915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9101..9689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9776..10570)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgaca tgaaagcctt cacactcgct ctcttcttag ctctttccct
     2701 ctatctcctg cccaatccag ccgcgccggt aaacacaact gacagcacag aaaacgttat
     2761 aaaacccttt atgcgtttta aagtccatat ggaggggagc gtcaatggac acgaatttga
     2821 gattgagggt gaaggagagg gcaagcctta cgagggaacc caaactgcga agctacaggt
     2881 gaccaagggc ggacccctcc catttgcttg ggacattctg tctccgcaat tccaatatgg
     2941 cagtaaagtg tatgtcaaac acccggccga cataccggac tacaagaagt tatcatttcc
     3001 ggaaggtttc aagtgggaga gagtcatgaa ttttgaagac gggggggtag taacagtgac
     3061 tcaggacagc agtcttcaag acggtacgtt catctatcat gtcaaattta tcggagtcaa
     3121 cttcccttct gacggaccgg ttatgcagaa gaagactctg ggctgggaac cgagtactga
     3181 gcgactgtac cctcgtgatg gagtactgaa aggtgagata cataaagcac taaagctcaa
     3241 gggcggcggt cattatctcg tggaatttaa gagtatttac atggctaaaa aacccgtaaa
     3301 gctacctgga tattattatg ttgattctaa actagatatt acgagccata acgaagacta
     3361 taccgtcgtc gagcagtatg agagagcaga ggcgcgacat catttattcc agggctcaag
     3421 cagaggagtt caggtcgaaa ctattagccc cggagatggc cgaacctttc cgaaaagggg
     3481 ccagactgta gttgtacact atacgggaat gctcgaggac ggaaagaagt tagacagctc
     3541 ccgagacaga aacaaaccgt tcaagttcat gctgggtaag caagaagtaa ttcgaggctg
     3601 ggaagaaggc gtagcccaaa tgagcgtagg acaacgagcc aaacttacga tttcaccaga
     3661 ttacgcctat ggggctactg gacatcccgg agtgatacct cctcatgcaa cgctcgtttt
     3721 tgatgttgag ttattgaagt tagagtaaac tagtccctag agtcctgctt taatgagata
     3781 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
     3841 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
     3901 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc
     3961 tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag
     4021 cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa
     4081 ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca
     4141 aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
     4201 ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
     4261 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
     4321 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
     4381 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
     4441 gaattttgcg acaacatgtc gaggctcagc aggacctgca ggcatgcaag cttggcactg
     4501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4621 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
     4681 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     4741 ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat
     4801 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     4861 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     4921 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     4981 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     5041 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     5101 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     5161 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     5221 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     5281 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     5341 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     5401 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     5461 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     5521 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     5581 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     5641 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     5701 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     5761 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     5821 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     5881 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     5941 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     6001 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     6061 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     6121 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     6181 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     6241 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     6301 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     6361 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     6421 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     6481 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     6541 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     6601 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     6661 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     6721 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     6781 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     6841 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     6901 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     6961 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     7021 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     7081 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     7141 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     7201 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     7261 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     7321 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     7381 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     7441 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     7501 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     7561 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     7621 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     7681 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     7741 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     7801 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     7861 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     7921 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     7981 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     8041 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     8101 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     8161 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     8221 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     8281 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     8341 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     8401 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     8461 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     8521 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     8581 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     8641 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8701 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8761 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     8821 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     8881 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     8941 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9001 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9061 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9121 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9181 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9241 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9301 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9361 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9421 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9481 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9541 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9601 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9661 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9721 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
     9781 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     9841 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
     9901 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     9961 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
    10021 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
    10081 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
    10141 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
    10201 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
    10261 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
    10321 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
    10381 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
    10441 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
    10501 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
    10561 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
    10621 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
    10681 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
    10741 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
    10801 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
    10861 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
    10921 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
    10981 tgttggctgg ctgg
//
