LOCUS       Exported                4444 bp ds-DNA     circular SYN 11-FEB-2020
DEFINITION  NotI mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     GenBank       4444 bp    DNA     circular      6-OCT-2006
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             7..702
                     /codon_start=1
                     /label=GSTTEVLIC
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDEN
                     LYFQSNIGSG"
     CDS             complement(2546..3403)
                     /codon_start=1
                     /label=Ampicillin\resistance\gene
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGGRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    3133..3134
                     /label=NotI site
                     /note="GA -> CG"
ORIGIN
        1 actagtatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
       61 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      121 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      181 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      241 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      301 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      361 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      421 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      481 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      541 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      601 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      661 tcggatgaga acctgtactt ccaatccaat attggaagtg gataacggat ccatatatag
      721 ggcccgggtt ataattacct caggtcgacg tcccatggtt ttgtatagaa tttacggcta
      781 gcgccggatg cgacgccggt cgcgtcttat ccggccttcc tatatcaggc tgtgtttaag
      841 acgccgccgc ttcgcccaaa tccttatgcc ggttcgacgg ctggacaaaa tactgtttat
      901 cttcccagcg caggcaggtt aatgtaccac cccagcagca gccggtatcc agcgcgtata
      961 taccttccgg cgtacctttg ccctccagcg atgcccagtg accaaaggcg atgctgtatt
     1021 cttcagcgac agggccagga atcgcaaacc acggtttcag tggggcaggg gcctcttccg
     1081 gcgattctta ctagctagta tgcataggtg ctgaaatata aagtttgtgt ttctaaaaca
     1141 cacgtggtac gtacgataac gtacagtgtt tttccctcca cttaaatcga agggtagtgt
     1201 cttggagcgc gcggagtaaa catatatggt tcatatatgt ccgtaggcac gtaaaaaaag
     1261 cgagggattc gaattccccc ggaacccccg gttggggccc acgcctcgat cgagcaaaaa
     1321 aaaaaaaaag aaaaaaaaaa aaaaaaaaag ctttcccgcg gccaagcttg gcgtaatcat
     1381 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1441 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1501 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1561 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1621 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1681 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1741 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1801 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1861 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1921 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1981 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2041 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2101 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2161 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2221 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2281 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2341 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2401 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2461 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2521 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2581 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2641 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2701 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2761 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2821 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2881 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2941 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3001 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3061 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3121 agtgtatgcg gccgccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3181 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3241 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3301 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3361 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3421 gttatgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3541 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3601 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3661 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3721 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3781 gcaccatatc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     3841 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     3901 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     3961 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     4021 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg
     4081 accctgctga ttggttcgct gaccatttcc ggggtgcgga acggcgttac cagaaactca
     4141 gaaggttcgt ccaaccaaac cgactctgac ggcagtttac gagagagatg atagggtctg
     4201 cttcagtaag ccagatgcta cacaattagg cttgtacata ttgtcgttag aacgcggcta
     4261 caattaatac ataaccttat gtatcataca catacgtaat acgactcact atagggtaca
     4321 cggaattcgc gtttttattt ttaattttct ttcaaatact tctagctaga gtatttttac
     4381 aacaattacc aacaacaaca acaaacaaca acaacattac attttacatt ctacaactac
     4441 agat
//
