LOCUS       Exported                3865 bp ds-DNA     circular SYN 10-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     misc_feature    97..126
                     /label=LIC site
     primer_bind     complement(224..245)
                     /label=pEU-rev
     rep_origin      complement(1205..1793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1964..2824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGGRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2825..2928)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3718..3736
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3728..3751
                     /label=pEU3-NII-for
     enhancer        3792..3862
                     /label=GAA Omega
ORIGIN
        1 actagtatgc atcatcatca tcatcattct cgtgcttggc gtcacccgca gttcggtggt
       61 actagtatgc atcatcatca tcatcatgag aacctgtact tccaatccaa tattggaagt
      121 ggataacgga tccatatata gggcccgggt tataattacc tcaggtcgac gtcccatggt
      181 tttgtataga atttacggct agcgccggat gcgacgccgg tcgcgtctta tccggccttc
      241 ctatatcagg ctgtgtttaa gacgccgccg cttcgcccaa atccttatgc cggttcgacg
      301 gctggacaaa atactgttta tcttcccagc gcaggcaggt taatgtacca ccccagcagc
      361 agccggtatc cagcgcgtat ataccttccg gcgtaccttt gccctccagc gatgcccagt
      421 gaccaaaggc gatgctgtat tcttcagcga cagggccagg aatcgcaaac cacggtttca
      481 gtggggcagg ggcctcttcc ggcgattctt actagctagt atgcataggt gctgaaatat
      541 aaagtttgtg tttctaaaac acacgtggta cgtacgataa cgtacagtgt ttttccctcc
      601 acttaaatcg aagggtagtg tcttggagcg cgcggagtaa acatatatgg ttcatatatg
      661 tccgtaggca cgtaaaaaaa gcgagggatt cgaattcccc cggaaccccc ggttggggcc
      721 cacgcctcga tcgagcaaaa aaaaaaaaaa gaaaaaaaaa aaaaaaaaaa gctttcccgc
      781 ggccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      841 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      901 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1201 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1261 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1321 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1381 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1441 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1501 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1561 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1621 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     1681 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1741 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1801 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1981 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2041 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2101 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2161 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2221 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2281 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2341 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2401 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2461 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2521 caaccaagtc attctgagaa tagtgtatgc ggccgccgag ttgctcttgc ccggcgtcaa
     2581 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2641 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2701 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2761 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2821 tcatactctt cctttttcaa tgttatgaag catttatcag ggttattgtc tcatgagcgg
     2881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2941 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3001 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     3061 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     3121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     3181 agagcagatt gtactgagag tgcaccatat cgacgctctc ccttatgcga ctcctgcatt
     3241 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     3301 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     3361 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     3421 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
     3481 tagaggatct ggctagcgat gaccctgctg attggttcgc tgaccatttc cggggtgcgg
     3541 aacggcgtta ccagaaactc agaaggttcg tccaaccaaa ccgactctga cggcagttta
     3601 cgagagagat gatagggtct gcttcagtaa gccagatgct acacaattag gcttgtacat
     3661 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgtaa
     3721 tacgactcac tatagggtac acggaattcg cgtttttatt tttaattttc tttcaaatac
     3781 ttctagctag agtattttta caacaattac caacaacaac aacaaacaac aacaacatta
     3841 cattttacat tctacaacta cagat
//
