LOCUS       Exported                3811 bp ds-DNA     circular SYN 10-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             10..33
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     misc_feature    43..72
                     /label=LIC site
     rep_origin      complement(1151..1739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1910..2770)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGGRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2771..2874)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3664..3682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(3690..3692,3738..3808)
                     /label=GAA Omega
ORIGIN
        1 actagtatgg attataagga cgacgacgac aaagagaacc tgtacttcca atccaatatt
       61 ggaagtggat aacggatcca tatatagggc ccgggttata attacctcag gtcgacgtcc
      121 catggttttg tatagaattt acggctagcg ccggatgcga cgccggtcgc gtcttatccg
      181 gccttcctat atcaggctgt gtttaagacg ccgccgcttc gcccaaatcc ttatgccggt
      241 tcgacggctg gacaaaatac tgtttatctt cccagcgcag gcaggttaat gtaccacccc
      301 agcagcagcc ggtatccagc gcgtatatac cttccggcgt acctttgccc tccagcgatg
      361 cccagtgacc aaaggcgatg ctgtattctt cagcgacagg gccaggaatc gcaaaccacg
      421 gtttcagtgg ggcaggggcc tcttccggcg attcttacta gctagtatgc ataggtgctg
      481 aaatataaag tttgtgtttc taaaacacac gtggtacgta cgataacgta cagtgttttt
      541 ccctccactt aaatcgaagg gtagtgtctt ggagcgcgcg gagtaaacat atatggttca
      601 tatatgtccg taggcacgta aaaaaagcga gggattcgaa ttcccccgga acccccggtt
      661 ggggcccacg cctcgatcga gcaaaaaaaa aaaaaagaaa aaaaaaaaaa aaaaaagctt
      721 tcccgcggcc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1561 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2461 agtactcaac caagtcattc tgagaatagt gtatgcggcc gccgagttgc tcttgcccgg
     2521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2761 gaatactcat actcttcctt tttcaatgtt atgaagcatt tatcagggtt attgtctcat
     2821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2881 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2941 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     3001 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     3061 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     3121 ggcatcagag cagattgtac tgagagtgca ccatatcgac gctctccctt atgcgactcc
     3181 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     3241 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     3301 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     3361 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     3421 ccggcgtaga ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg
     3481 gtgcggaacg gcgttaccag aaactcagaa ggttcgtcca accaaaccga ctctgacggc
     3541 agtttacgag agagatgata gggtctgctt cagtaagcca gatgctacac aattaggctt
     3601 gtacatattg tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
     3661 acgtaatacg actcactata gggtacacgg aattcgcgtt tttattttta attttctttc
     3721 aaatacttct agctagagta tttttacaac aattaccaac aacaacaaca aacaacaaca
     3781 acattacatt ttacattcta caactacaga t
//
