LOCUS       Exported                4456 bp ds-DNA     circular SYN 10-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             7..660
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             667..687
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     misc_feature    676..705
                     /label=LIC site
     CDS             714..731
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(815..836)
                     /label=pEU-rev
     rep_origin      complement(1796..2384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2555..3415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGGRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3416..3519)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4309..4327
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4319..4342
                     /label=pEU3-NII-for
     enhancer        4383..4453
                     /label=GAA Omega
ORIGIN
        1 actagtatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
       61 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      121 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      181 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      241 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      301 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      361 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      421 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      481 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      541 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      601 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      661 tcggatgaga acctgtactt ccaatccaat attggaagtg gataacggat ccccatcatc
      721 atcatcatca ttgacccggg ttataattac ctcaggtcga cgtcccatgg ttttgtatag
      781 aatttacggc tagcgccgga tgcgacgccg gtcgcgtctt atccggcctt cctatatcag
      841 gctgtgttta agacgccgcc gcttcgccca aatccttatg ccggttcgac ggctggacaa
      901 aatactgttt atcttcccag cgcaggcagg ttaatgtacc accccagcag cagccggtat
      961 ccagcgcgta tataccttcc ggcgtacctt tgccctccag cgatgcccag tgaccaaagg
     1021 cgatgctgta ttcttcagcg acagggccag gaatcgcaaa ccacggtttc agtggggcag
     1081 gggcctcttc cggcgattct tactagctag tatgcatagg tgctgaaata taaagtttgt
     1141 gtttctaaaa cacacgtggt acgtacgata acgtacagtg tttttccctc cacttaaatc
     1201 gaagggtagt gtcttggagc gcgcggagta aacatatatg gttcatatat gtccgtaggc
     1261 acgtaaaaaa agcgagggat tcgaattccc ccggaacccc cggttggggc ccacgcctcg
     1321 atcgagcaaa aaaaaaaaaa agaaaaaaaa aaaaaaaaaa agctttcccg cggccaagct
     1381 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1441 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1501 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1561 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1621 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2221 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2701 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2761 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2821 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2881 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2941 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3001 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3061 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3121 cattctgaga atagtgtatg cggccgccga gttgctcttg cccggcgtca atacgggata
     3181 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3241 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3301 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3361 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3421 tcctttttca atgttatgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3481 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3541 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3601 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     3661 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     3721 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     3781 tgtactgaga gtgcaccata tcgacgctct cccttatgcg actcctgcat taggaagcag
     3841 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     3901 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     3961 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     4021 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     4081 tggctagcga tgaccctgct gattggttcg ctgaccattt ccggggtgcg gaacggcgtt
     4141 accagaaact cagaaggttc gtccaaccaa accgactctg acggcagttt acgagagaga
     4201 tgatagggtc tgcttcagta agccagatgc tacacaatta ggcttgtaca tattgtcgtt
     4261 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgta atacgactca
     4321 ctatagggta cacggaattc gcgtttttat ttttaatttt ctttcaaata cttctagcta
     4381 gagtattttt acaacaatta ccaacaacaa caacaaacaa caacaacatt acattttaca
     4441 ttctacaact acagat
//
