LOCUS       pCRI012-pGEM-Pac        3711 bp    DNA     circular UNA 11-SEP-2020
DEFINITION  Roux et al 2020 vector set.
ACCESSION   urn.local...qt-ce7jk13
VERSION     urn.local...qt-ce7jk13
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    112..119
                     /note="Geneious type: misc. marker"
                     /standard_name="PacI"
     promoter        <121..587
                     /standard_name="AfPU3"
     unsure          589..594
     misc_feature    595..714
                     /note="Geneious type: motif"
                     /standard_name="lacZ fragment"
     unsure          715..720
     ncRNA           721..797
                     /standard_name="Cas9 sgRNA scaffold"
     terminator      798..805
                     /standard_name="Poly T"
     misc_feature    812..819
                     /note="Geneious type: misc. marker"
                     /standard_name="NotI"
     CDS             complement(<832..916)
                     /standard_name="5'-lacZa"
     rep_origin      complement(1299..1918)
     gene            complement(2073..2933)
                     /gene="Ampicillin"
                     /standard_name="Ampicillin "
     promoter        complement(2975..3003)
                     /standard_name="AmpR_promoter"
     rep_origin      complement(3248..3554)
     misc_feature    complement(3583..>3711)
                     /standard_name="lacZ_a"
ORIGIN      
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg aattcctcgt cgtgtcgccc attaattaag
      121 atcacataga tgctcggttg acaggaccac ctatagcaag tactttgtag gaggttctcc
      181 taacgcttgg cttcaaatcc aatcaggata tcagtgagtt actgtttgtc atcgcatcct
      241 catgaagtcg ctcaacagcc actgagaagc aaatatttgg gagaccatcc ctgatgttga
      301 aattttacgc tggagcccat tcaccggtga gctcggggac agtctggcca gtggagcgga
      361 aaatcttcaa ctaactctga ttggtaccac aggtagcccc acagaacaaa ccgagcaaac
      421 atgaaaattt tcgcttgagg ttagcgcact cgctagcgcc tgcccgcaaa tataaaaggc
      481 cccgaaatcc catgcatttt ggaaatcaag cgactctacg tatgtcatcc ccctccctac
      541 ctttgggaaa acctctcaac tgcaagtcag aacattttgc taacagcaga gacgggcgcc
      601 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      661 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgcgtctc
      721 cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      781 tggcaccgag tcggtgcttt tttttcaatt cgcggccgcc tgcaggtcga ccatatggga
      841 gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg
      901 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      961 aacatacgag ccggaagcat aaagtgcaaa gcctggggtg cctaatgagt gagctaactc
     1021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3061 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     3121 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3181 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3241 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3301 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3361 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3421 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3481 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3541 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     3601 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3661 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt t
//
