LOCUS       pMAC118                 5733 bp ds-DNA     circular     07-MAR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     2466..2486
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        5098..5345
                     /label="3' PB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     2440..2457
                     /label="M13-fwd primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2467..2485
                     /label="T7 promoter (Ambion)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     polyA_signal    4833..5057
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(5384..5403)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1976..2282)
                     /label="F1 origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(5450..5467)
                     /label="E coli lacZp1 UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(853..1713)
                     /label="beta-lactamase"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3367..4584
                     /label="dLbCpf1 N 406"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4591..4767
                     /label="10Glycine-EE12RR345L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     prot_bind       5449..5465
                     /label="LacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(856..1515)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2301..2369)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(5450..5501)
                     /label="E coli lacZp4 UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     prot_bind       5518..5539
                     /label="CAPbs"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(4827..4844)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(4789..4807)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2441..2457
                     /label="M13F"
                     /ApEinfo_revcolor=#cd3249
                     /ApEinfo_fwdcolor=#cd3249
     primer_bind     complement(5421..5441)
                     /label="M13-rev primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(5425..5441)
                     /label="M13R"
                     /ApEinfo_revcolor=#cd3249
                     /ApEinfo_fwdcolor=#cd3249
     promoter        complement(5386..5404)
                     /label="T3p"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    complement(5447..5469)
                     /label="LacO site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1755..1783)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(76..758)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(5520..5539)
                     /label="CRP binding site (lacZYA MG1655)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(5473..5503)
                     /label="LACp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(94..682)
                     /label="ORI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(1714..1818)
                     /label="AmpRp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        2467..2485
                     /label="T7p"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        2854..3353
                     /label="PGK promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(4790..4807)
                     /label="SP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        2511..2832
                     /label="5' PB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(1844..2299)
                     /label="ORI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
       61 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     1861 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1921 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1981 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2041 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2101 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2161 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2221 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2281 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2341 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2401 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     2461 cgcgcgtaat acgactcact atagggcgaa ttggggcgcg ccattctaga ttaaccctag
     2521 aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa
     2581 tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga
     2641 ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca
     2701 aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat
     2761 tgttatttca tgttctactt acgtgataac ttattatata tatattttct tgttatagat
     2821 atcaactaga atgctagcat gggcccagat ctcGGGTAGG GGAGGCGCTT TTCCCAAGGC
     2881 AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT
     2941 GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC
     3001 CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG
     3061 CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA
     3121 GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT
     3181 CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG
     3241 GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC
     3301 AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGaGAATTC
     3361 GGTACCatga gcaagctgga gaagtttaca aactgctact ccctgtctaa gaccctgagg
     3421 ttcaaggcca tccctgtggg caagacccag gagaacatcg acaataagcg gctgctggtg
     3481 gaggacgaga agagagccga ggattataag ggcgtgaaga agctgctgga tcgctactat
     3541 ctgtctttta tcaacgacgt gctgcacagc atcaagctga agaatctgaa caattacatc
     3601 agcctgttcc ggaagaaaac cagaaccgag aaggagaata aggagctgga gaacctggag
     3661 atcaatctgc ggaaggagat cgccaaggcc ttcaagggca acgagggcta caagtccctg
     3721 tttaagaagg atatcatcga gacaatcctg ccagagttcc tggacgataa ggacgagatc
     3781 gccctggtga acagcttcaa tggctttacc acagccttca ccggcttctt tgataacaga
     3841 gagaatatgt tttccgagga ggccaagagc acatccatcg ccttcaggtg tatcaacgag
     3901 aatctgaccc gctacatctc taatatggac atcttcgaga aggtggacgc catctttgat
     3961 aagcacgagg tgcaggagat caaggagaag atcctgaaca gcgactatga tgtggaggat
     4021 ttctttgagg gcgagttctt taactttgtg ctgacacagg agggcatcga cgtgtataac
     4081 gccatcatcg gcggcttcgt gaccgagagc ggcgagaaga tcaagggcct gaacgagtac
     4141 atcaacctgt ataatcagaa aaccaagcag aagctgccta agtttaagcc actgtataag
     4201 caggtgctga gcgatcggga gtctctgagc ttctacggcg agggctatac atccgatgag
     4261 gaggtgctgg aggtgtttag aaacaccctg aacaagaaca gcgagatctt cagctccatc
     4321 aagaagctgg agaagctgtt caagaatttt gacgagtact ctagcgccgg catctttgtg
     4381 aagaacggcc ccgccatcag cacaatctcc aaggatatct tcggcgagtg gaacgtgatc
     4441 cgggacaagt ggaatgccga gtatgacgat atccacctga agaagaaggc cgtggtgacc
     4501 gagaagtacg aggacgatcg gagaaagtcc ttcaagaaga tcggctcctt ttctctggag
     4561 cagctgcagg agtacgccga cgccAAGCTT ggaggaggtg gtggaggtgg aggtggaggt
     4621 actagtctgg agatcgaggc cgccttcctg gagcgcgaga acaccgccct ggagacccgc
     4681 gtggccgagc tgcgccagcg cgtgcagcgc ctgcgcaacc gcgtgagcca gaaccgcacc
     4741 cgctacggcc ccctgggcgg cggcaagtaa CTCGAGagag ggccctattc tatagtgtca
     4801 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4861 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4921 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4981 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5041 tgcggtgggc tctatggctt ctgaggTCGA GGCGGCCGCT GATCACAATT GTCTAGTtaa
     5101 aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa
     5161 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact
     5221 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc
     5281 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaat
     5341 ctagtatacg cgtatgcggc cgcttaatta atccagcttt tgttcccttt agtgagggtt
     5401 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5701 ggtatcagct cactcaaagg cggtaatacg gtt
//