LOCUS       pMAC236                 7332 bp ds-DNA     circular     20-NOV-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(94..682)
                     /label="ORI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(1976..2282)
                     /label="F1 origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(1844..2299)
                     /label="ORI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     prot_bind       7048..7064
                     /label="LacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(6389..6406)
                     /label="SP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3553..6018
                     /label="dLbCpf1 C 406"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(7119..7138)
                     /label="CRP binding site (lacZYA MG1655)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(856..1515)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(7049..7100)
                     /label="E coli lacZp4 UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     prot_bind       7117..7138
                     /label="CAPbs"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(7072..7102)
                     /label="LACp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        2854..3353
                     /label="PGK promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(7024..7040)
                     /label="M13R"
                     /ApEinfo_revcolor=#cd3249
                     /ApEinfo_fwdcolor=#cd3249
     primer_bind     complement(7020..7040)
                     /label="M13-rev primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2301..2369)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2441..2457
                     /label="M13F"
                     /ApEinfo_revcolor=#cd3249
                     /ApEinfo_fwdcolor=#cd3249
     promoter        6697..6944
                     /label="3' PB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(853..1713)
                     /label="beta-lactamase"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(1714..1818)
                     /label="AmpRp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6025..6051
                     /label="HA tag"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     polyA_signal    6432..6656
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        2467..2485
                     /label="T7p"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(6388..6406)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3367..3546
                     /label="RR12EE345L-10Glycine"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6686..6693
                     /label="backbone"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(6983..7002)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1755..1783)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(7049..7066)
                     /label="E coli lacZp1 UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6674..6685
                     /label="backbone"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_binding    complement(7046..7068)
                     /label="LacO site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(6426..6443)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(6985..7003)
                     /label="T3p"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(76..758)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2467..2485
                     /label="T7 promoter (Ambion)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6061..6366
                     /label="N intein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2466..2486
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2511..2832
                     /label="5' PB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     2440..2457
                     /label="M13-fwd primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
       61 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     1861 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1921 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1981 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2041 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2101 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2161 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2221 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2281 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2341 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2401 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     2461 cgcgcgtaat acgactcact atagggcgaa ttggggcgcg ccattctaga ttaaccctag
     2521 aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa
     2581 tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga
     2641 ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca
     2701 aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat
     2761 tgttatttca tgttctactt acgtgataac ttattatata tatattttct tgttatagat
     2821 atcaactaga atgctagcat gggcccagat ctcGGGTAGG GGAGGCGCTT TTCCCAAGGC
     2881 AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT
     2941 GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC
     3001 CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG
     3061 CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA
     3121 GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT
     3181 CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG
     3241 GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC
     3301 AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGaGAATTC
     3361 GGTACCatgc tggagatccg cgccgccttc ctgcgccagc gcaacaccgc cctgcgcacc
     3421 gaggtggccg agctggagca ggaggtgcag cgcctggaga acgaggtgag ccagtacgag
     3481 acccgctacg gccccctggg cggcggcaag gctagcggag gaggtggtgg aggtggaggt
     3541 ggaggtcata tggatctgtc tgtggtggag aagctgaagg agatcatcat ccagaaggtg
     3601 gatgagatct acaaggtgta tggctcctct gagaagctgt tcgacgccga ttttgtgctg
     3661 gagaagagcc tgaagaagaa cgacgccgtg gtggccatca tgaaggacct gctggattct
     3721 gtgaagagct tcgagaatta catcaaggcc ttctttggcg agggcaagga gacaaacagg
     3781 gacgagtcct tctatggcga ttttgtgctg gcctacgaca tcctgctgaa ggtggaccac
     3841 atctacgatg ccatccgcaa ttatgtgacc cagaagccct actctaagga taagttcaag
     3901 ctgtattttc agaaccctca gttcatgggc ggctgggaca aggataagga gacagactat
     3961 cgggccacca tcctgagata cggctccaag tactatctgg ccatcatgga taagaagtac
     4021 gccaagtgcc tgcagaagat cgacaaggac gatgtgaacg gcaattacga gaagatcaac
     4081 tataagctgc tgcccggccc taataagatg ctgccaaagg tgttcttttc taagaagtgg
     4141 atggcctact ataaccccag cgaggacatc cagaagatct acaagaatgg cacattcaag
     4201 aagggcgata tgtttaacct gaatgactgt cacaagctga tcgacttctt taaggatagc
     4261 atctcccggt atccaaagtg gtccaatgcc tacgatttca acttttctga gacagagaag
     4321 tataaggaca tcgccggctt ttacagagag gtggaggagc agggctataa ggtgagcttc
     4381 gagtctgcca gcaagaagga ggtggataag ctggtggagg agggcaagct gtatatgttc
     4441 cagatctata acaaggactt ttccgataag tctcacggca cacccaatct gcacaccatg
     4501 tacttcaagc tgctgtttga cgagaacaat cacggacaga tcaggctgag cggaggagca
     4561 gagctgttca tgaggcgcgc ctccctgaag aaggaggagc tggtggtgca cccagccaac
     4621 tcccctatcg ccaacaagaa tccagataat cccaagaaaa ccacaaccct gtcctacgac
     4681 gtgtataagg ataagaggtt ttctgaggac cagtacgagc tgcacatccc aatcgccatc
     4741 aataagtgcc ccaagaacat cttcaagatc aatacagagg tgcgcgtgct gctgaagcac
     4801 gacgataacc cctatgtgat cggcatcgcc aggggcgagc gcaatctgct gtatatcgtg
     4861 gtggtggacg gcaagggcaa catcgtggag cagtattccc tgaacgagat catcaacaac
     4921 ttcaacggca tcaggatcaa gacagattac cactctctgc tggacaagaa ggagaaggag
     4981 aggttcgagg cccgccagaa ctggacctcc atcgagaata tcaaggagct gaaggccggc
     5041 tatatctctc aggtggtgca caagatctgc gagctggtgg agaagtacga tgccgtgatc
     5101 gccctggagg acctgaactc tggctttaag aatagccgcg tgaaggtgga gaagcaggtg
     5161 tatcagaagt tcgagaagat gctgatcgat aagctgaact acatggtgga caagaagtct
     5221 aatccttgtg caacaggcgg cgccctgaag ggctatcaga tcaccaataa gttcgagagc
     5281 tttaagtcca tgtctaccca gaacggcttc atcttttaca tccctgcctg gctgacatcc
     5341 aagatcgatc catctaccgg ctttgtgaac ctgctgaaaa ccaagtatac cagcatcgcc
     5401 gattccaaga agttcatcag ctcctttgac aggatcatgt acgtgcccga ggaggatctg
     5461 ttcgagtttg ccctggacta taagaacttc tctcgcacag acgccgatta catcaagaag
     5521 tggaagctgt actcctacgg caaccggatc agaatcttcc ggaatcctaa gaagaacaac
     5581 gtgttcgact gggaggaggt gtgcctgacc agcgcctata aggagctgtt caacaagtac
     5641 ggcatcaatt atcagcaggg cgatatcaga gccctgctgt gcgagcagtc cgacaaggcc
     5701 ttctactcta gctttatggc cctgatgagc ctgatgctgc agatgcggaa cagcatcaca
     5761 ggccgcaccg acgtggattt tctgatcagc cctgtgaaga actccgacgg catcttctac
     5821 gatagccgga actatgaggc ccaggagaat gccatcctgc caaagaacgc cgacgccaat
     5881 ggcgcctata acatcgccag aaaggtgctg tgggccatcg gccagttcaa gaaggccgag
     5941 gacgagaagc tggataaggt gaagatcgcc atctctaaca aggagtggct ggagtacgcc
     6001 cagaccagcg tgaagcacgg atccTATCCC TATGACGTGC CCGATTATGC Cagcctgggc
     6061 TGTCTGTCAT ACGAAACTGA GATACTGACG GTGGAATATG GTCTCCTTCC TATTGGGAAA
     6121 ATAGTGGAAA AGAGGATCGA GTGTACGGTT TACTCAGTGG ACAACAATGG GAATATCTAT
     6181 ACGCAACCTG TCGCTCAGTG GCATGATCGA GGGGAACAGG AGGTGTTTGA GTACTGTTTG
     6241 GAGGACGGTT CTCTTATTCG GGCTACTAAG GACCACAAGT TTATGACAGT TGATGGTCAG
     6301 ATGTTGCCCA TCGACGAGAT ATTTGAGCGG GAACTCGATT TGATGCGGGT TGATAATCTG
     6361 CCGAATtaaC TCGAGagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
     6421 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     6481 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     6541 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6601 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6661 tgaggTCGAG GCGGCCGCTG ATCACAATTG TCTAGTtaaa agttttgtta ctttatagaa
     6721 gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg
     6781 aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata
     6841 aataaacctc gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt
     6901 taacgtacgt cacaatatga ttatctttct agggttaatc tagtatacgc gtatgcggcc
     6961 gcttaattaa tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     7021 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7321 ggtaatacgg tt
//