LOCUS       pMAC237                 6138 bp ds-DNA     circular     21-OCT-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             3481..3528
                     /label="2x NLS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3748..4233
                     /label="mini p65 AD domain (church paper)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     prot_bind       5854..5870
                     /label="LacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(76..758)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     polyA_signal    5238..5462
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3565..3720
                     /label="VP64 AD domain"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4252..4449
                     /label="mini RTA (church paper)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(5826..5846)
                     /label="M13-rev primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(1714..1818)
                     /label="AmpRp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(5830..5846)
                     /label="M13R"
                     /ApEinfo_revcolor=#cd3249
                     /ApEinfo_fwdcolor=#cd3249
     misc_feature    3529..3561
                     /label="linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(5194..5212)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     2440..2457
                     /label="M13-fwd primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4234..4251
                     /label="linker(1)"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     promoter        complement(5791..5809)
                     /label="T3p"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2467..2485
                     /label="T7 promoter (Ambion)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(5195..5212)
                     /label="SP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        2467..2485
                     /label="T7p"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(2301..2369)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(5855..5872)
                     /label="E coli lacZp1 UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        2854..3353
                     /label="PGK promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(5855..5906)
                     /label="E coli lacZp4 UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        5503..5750
                     /label="3' PB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2441..2457
                     /label="M13F"
                     /ApEinfo_revcolor=#cd3249
                     /ApEinfo_fwdcolor=#cd3249
     misc_feature    4465..5175
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(5789..5808)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(5232..5249)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    complement(5852..5874)
                     /label="LacO site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(5925..5944)
                     /label="CRP binding site (lacZYA MG1655)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(5878..5908)
                     /label="LACp"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(94..682)
                     /label="ORI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(856..1515)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(853..1713)
                     /label="beta-lactamase"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        2511..2832
                     /label="5' PB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3721..3741
                     /label="NLS signal"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3367..3471
                     /label="C intein"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(1976..2282)
                     /label="F1 origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     prot_bind       5923..5944
                     /label="CAPbs"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(1844..2299)
                     /label="ORI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     2466..2486
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1755..1783)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
       61 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     1861 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1921 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1981 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2041 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2101 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2161 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2221 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2281 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2341 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2401 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     2461 cgcgcgtaat acgactcact atagggcgaa ttggggcgcg ccattctaga ttaaccctag
     2521 aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa
     2581 tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga
     2641 ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca
     2701 aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat
     2761 tgttatttca tgttctactt acgtgataac ttattatata tatattttct tgttatagat
     2821 atcaactaga atgctagcat gggcccagat ctcGGGTAGG GGAGGCGCTT TTCCCAAGGC
     2881 AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT
     2941 GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC
     3001 CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG
     3061 CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA
     3121 GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT
     3181 CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG
     3241 GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC
     3301 AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGaGAATTC
     3361 GGTACCATGA TAAAGATCGC CACTCGGAAG TATTTGGGGA AACAGAACGT GTATGATATT
     3421 GGTGTCGAGA GAGACCACAA CTTTGCACTT AAGAATGGGT TCATAGCGTC Tagcggctcc
     3481 cccaagaaaa aacgcaaggt ggaagatcct aagaaaaagc ggaaagtgGA CGGCATTGGT
     3541 AGTGGGAGCA ACGGCAGCAG CCTCGATGCT TTAGACGATT TTGACTTAGA TATGCTTGGT
     3601 TCAGACGCGT TAGACGACTT CGACCTAGAC ATGTTAGGCT CAGATGCATT GGACGACTTC
     3661 GATTTAGATA TGTTGGGCTC CGATGCCCTA GATGACTTTG ATCTAGATAT GCTAGGTAGT
     3721 CCCAAAAAGA AGAGGAAAGT GGGATCCGTC CTTCCGCAAG CGCCTGCCCC AGCGCCTGCA
     3781 CCGGCAATGG TGTCCGCCCT CGCACAGGCC CCTGCGCCCG TCCCCGTGCT CGCGCCTGGA
     3841 CCGCCCCAGG CGGTCGCTCC ACCGGCTCCG AAGCCGACGC AGGCCGGAGA GGGAACACTC
     3901 TCCGAAGCAC TTCTTCAACT CCAGTTTGAT GACGAGGATC TTGGAGCACT CCTTGGAAAC
     3961 TCGACAGACC CTGCGGTGTT TACCGACCTC GCGTCAGTAG ATAACTCCGA ATTTCAGCAG
     4021 CTTTTGAACC AGGGTATCCC GGTCGCGCCA CATACAACGG AGCCCATGTT GATGGAATAC
     4081 CCCGAAGCAA TCACGAGACT TGTGACGGGA GCGCAGCGGC CTCCCGATCC CGCACCCGCA
     4141 CCTTTGGGGG CACCTGGCCT CCCTAACGGA CTTTTGAGCG GCGACGAGGA TTTCTCCTCC
     4201 ATCGCCGATA TGGATTTCTC AGCCTTGCTG TCAGGCTCTG GCAGCGGCAG CGACCTTTCC
     4261 CATCCGCCCC CAAGGGGCCA TCTGGATGAG CTGACAACCA CACTTGAGTC CATGACCGAG
     4321 GATCTGAACC TGGACTCACC CCTGACCCCG GAATTGAACG AGATTCTGGA TACCTTCCTG
     4381 AACGACGAGT GCCTCTTGCA TGCCATGCAT ATCAGCACAG GACTGTCCAT CTTCGACACA
     4441 TCTCTGTTTG GCAGCAGCGG ATCCatggtg agcaagggcg aggaggataa catggccatc
     4501 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     4561 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     4621 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     4681 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     4741 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     4801 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     4861 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     4921 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     4981 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     5041 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     5101 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     5161 gagctgtaca agtaaCTCGA Gagagggccc tattctatag tgtcacctaa atgctagagc
     5221 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5281 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5341 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5401 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5461 ggcttctgag gTCGAGGCGG CCGCTGATCA CAATTGTCTA GTtaaaagtt ttgttacttt
     5521 atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt
     5581 ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa
     5641 ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat
     5701 tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagt atacgcgtat
     5761 gcggccgctt aattaatcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     5821 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5881 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5941 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6001 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6061 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6121 aaaggcggta atacggtt
//