LOCUS       pMAC211                 8858 bp ds-DNA     circular     13-FEB-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     misc_feature    574..592
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     8814..8834
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    538..556
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(4144..4197)
                     /label="T2A (EGRGSLLTCGDVEENPG/P)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    574..592
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2452..2471
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3385..4131)
                     /label="rtTA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    394..412
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(8103..8131)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1712..2390
                     /label="FLuc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    394..412
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             738..1711
                     /label="FLuc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5724..5958
                     /label="PiggyBac L-border"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(6135..6154)
                     /label="CRP binding site (lacZYA MG1655)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1..313
                     /label="PiggyBac R-border"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(7204..7863)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(6036..6056)
                     /label="M13-rev primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     8788..8805
                     /label="M13-fwd primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    502..520
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    466..484
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    466..484
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    8815..8833
                     /label="T7 promoter (Ambion)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2554..2685
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    358..376
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2642..2661
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2429..2451
                     /label="crIFNg_5"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    430..448
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2409..2428
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(5999..6018)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    358..376
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    502..520
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    430..448
                     /label="TetO2 site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2495..2514
                     /label="crRNA repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2472..2494
                     /label="crIFNg_5"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    538..556
                     /label="Tetracycline Response Element (TRE)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_binding    complement(6062..6084)
                     /label="LacO site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(8649..8717)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(6424..7106)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(8324..8630)
                     /label="F1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(4207..4578)
                     /label="bleo gene"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcaactaga atgctagcgc tgatatctcc accccacagt ggtttactcc
      361 ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga gaacgtatgc
      421 agactttact ccctatcagt gatagagaac gtataaggag tttactccct atcagtgata
      481 gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac agtttactcc
      541 ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga gaacgtataa
      601 gctttaggcg tgtacggtgg gcgcctataa aagcagagct cgtttagtga accgtcagat
      661 cgcctggagc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
      721 ctcgtaaaga attcaccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct
      781 atccgctaga ggatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc
      841 tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc acgtacgcgg
      901 aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa
      961 atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg
     1021 cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc
     1081 tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa
     1141 aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc atggattcta
     1201 aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg
     1261 gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca attgcactga
     1321 taatgaactc ctctggatct actgggttac ctaagggtgt ggcccttccg catagaactg
     1381 cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata
     1441 ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat
     1501 atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac
     1561 gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta ttttcattct
     1621 tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg
     1681 ggggcgcacc tctttcgaaa gaagtcgggg aggcggttgc aaaacgcttc catcttccag
     1741 ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg
     1801 gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg
     1861 atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt gtcagaggac
     1921 ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg
     1981 atggatggct acattctgga gacatagctt actgggacga agacgaacac ttcttcatag
     2041 ttgaccgctt gaagtcttta attaaataca aaggatacca ggtggccccc gctgaattgg
     2101 agtcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt cttcccgacg
     2161 atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg
     2221 aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag
     2281 gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa
     2341 tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa GGATCCTTTC
     2401 TTTCTTTGaa tttctactaa gtgtagatag atgagatggt gacagatagg cAATTTCTAC
     2461 TAAGTGTAGA Tagatgagat ggtgacagat aggcAATTTC TACTAAGTGT AGATCGAGTT
     2521 TTCTTTTTGT TTtgtacaag taagttACGC GTcttgttta ttgcagctta taatggttac
     2581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2641 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     2701 gctaaccgtc atacatatgt cgacggtatc gatgcgggga ggcggcccaa agggagatcc
     2761 gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc
     2821 gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac gtccccgcgc
     2881 agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac
     2941 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
     3001 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
     3061 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
     3121 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
     3181 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
     3241 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
     3301 attttgtaat ccagaggttg attatcgata agcttgatat caagcttgca tgcctgcagg
     3361 tcgactctag agtcttatgc ggccttagcc tggcagcata tcgagatcga agtcatcgag
     3421 agcgtcagca gggagcatat ccagatcaaa atcatccagg gcatcggcgg gcagcatgtc
     3481 caggtcgaag tcgtccaggg cgtcggtggg tccgccgctc tcgcacttca gctgcttctc
     3541 gaggccgcag atgatcagtt ccaggccgaa caggaaggcg ggctcggcgc cctgtctgtc
     3601 gaacagctcg atggcctgct tcagcagggg gggcatgctg tcggtggtgg gtgtctctct
     3661 ttcctctttg gcgacctggt gttcctgttc ttccagcacg cagcccaggg tgaagtggcc
     3721 cacggcgctc agggcgtaca gggcgttttc caggctgaag ccctgctggc acaggaaggc
     3781 cagctggttt tccagtgtct cgtactgctt ctcggtgggt ctggtgccca ggtgcacttt
     3841 ggcgccgtcc cggtggctca gcagggcgca tctgtagctc ttggcgttgt tccgcaggaa
     3901 gtcctgccag ctctcgcctt ccagagggca gctgtgggtg tggtgccggt ccagcatctc
     3961 gatgggcagg gcgtccagca gggcccgctt gttcttcacg tgccagtaca gggtgggctg
     4021 ttccacgccc agtttctggg ccagcttccg ggtggtcagg ccctcgatgc ccacgccgtt
     4081 cagcagttcc agggcgctgt tgatcacttt gctcttgtcc agccggctca tcgaccctcc
     4141 gcctgggcca ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctcgga
     4201 accgccgtcc tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc
     4261 gaactcccgc ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg
     4321 gaagttcgtg gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca
     4381 cacccaggcc agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt
     4441 cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag
     4501 ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc
     4561 actggtcaac ttggccataa ttctcgagag ctccagatcc cctgttcacg acacctgaaa
     4621 tggaagaaaa aaactttgaa ccactgtctg aggcttgaga atgaaccaag atccaaactc
     4681 aaaaaggcca aattccaagg agaattacat caagtgccaa gctggcctaa cttcagtctc
     4741 cacccactca gtgtggggaa actccatcgc ataaaacccc tccccccaac ctaaagacga
     4801 cgtactccaa aagctccaga actaatcgag gtgcctggac ggcgcccggt actccgtgga
     4861 gtcacatgaa gcgacggctg aggacggaaa ggcccttttc ctttgtgtgg gtgactcacc
     4921 cgcccgctct cccgagcgcc gcgtcctcca ttttgagccc cctggagcag ggccgggaag
     4981 cggccatctt tccgctcacg caactggtgc cgaccgggcc agccttgccg cccagggcgg
     5041 ggcgatacac ggcggcgcga ggccaggcac cagagcaggc cggccagctt gagactaccc
     5101 ccgtccgatt ctcggtggcc gcgctcgcag gccccgcctc gccgaacatg tgcgctggga
     5161 cgcacgggcc ccgtcgccgg ccgcgggccc aaaaaccgaa ataccagtgt gcagatcctg
     5221 gcccgcattt acaagactat cttgccagaa aaaaagcgtc gcagcacgtc atcaaaaatt
     5281 ttaaatggct agagacttat cgaaagcagc gagacaggcg cgaaggtgcc accagattcg
     5341 cacgcggcgg ccccagcgcc caggccaggc ctcaactcaa gcacgaggcg aaggggctcc
     5401 taaagcgcaa ggcccgcccc tggctccagc tcgggatcaa gaatcacgta ctggagccag
     5461 gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa agaggccagg cccgcgggaa
     5521 ccacacacgg cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg
     5581 gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg gagccagtac
     5641 acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt tcaattggct
     5701 gcaggactag tctagttaaa agttttgtta ctttatagaa gaaattttga gtttttgttt
     5761 ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa
     5821 gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc gatatacaga
     5881 ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga
     5941 ttatctttct agggttaatc tagtgtttaa acggccgctt aattaatcca gcttttgttc
     6001 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6841 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8161 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     8221 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     8281 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     8341 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     8401 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8461 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8521 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8581 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8641 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8701 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8761 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     8821 gactcactat agggcgaatt ggggcgcgcc attctaga
//