LOCUS       pRZ5150_=_SPACE_NG       10308 bp ds-DNA     circular SYN 14-FEB-2020
DEFINITION  pCMV-bpNLS-TadA*(V82G)-nCas9_NG-bpNLS-pmCDA1(R187W)-UGI-UGI-P2A-EGF
            P.
ACCESSION
VERSION     .
KEYWORDS    pCMV_ABEmax_P2A_GFP.
SOURCE      
  ORGANISM  .
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Koblan, LW, Doman, JL, Wilson, C, Levy, JM, Tay, T, Newby, GA,
            Maianti, JP, Raguram, A, Liu and DR
  TITLE     Improving cytidine and adenine base editors by expression
            optimization and ancestral reconstruction.
  JOURNAL   Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
  PUBMED    29813047
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     286..306
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     primer_bind     343..368
                     /Sequence="TGAACCGTCAGATCCGCTAGAGATCC"
                     /Hairpin_Tm="None"
                     /Tm="65.5"
                     /Self_Dimer_Tm="15.8"
                     /%GC="53.8"
                     /modified_by="User"
                     /label="oRZ176"
     primer_bind     371..374
                     /Sequence="GGCC"
                     /Color="#ff33ff"
                     /modified_by="User"
                     /label="cut BB"
     promoter        377..395
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     377..396
                     /note="T7 promoter, forward primer"
                     /label="T7"
     motif           400..411
                     /created_by="User"
                     /label="KOZAC"
     primer_bind     412..465
                     /created_by="User"
                     /Sequence="AAACGGACAGCCGACGGAAGCGAGTTCGAGTCACCAAAGAAGAAGCG
                     GAAAGTC"
                     /Color="#1199ff"
                     /modified_by="User"
                     /label="NLS"
     misc_feature    465^466
                     /Original_Bases="TCTGAAGTCGAGTTTAGCCACGAGTATTGGATGAGGCACGC
                     ACTGACCCTGGCAAAGCGAGCATGGGATGAAAGAGAAGTCCCCGTGGGCGCCGTGCTG
                     GTGCACAACAATAGAGTGATCGGAGAGGGATGGAACAGGCCAATCGGCCGCCACGACC
                     CTACCGCACACGCAGAGATCATGGCACTGAGGCAGGGAGGCCTGGTCATGCAGAATTA
                     CCGCCTGATCGATGCCACCCTGTATGTGACACTGGAGCCATGCGTGATGTGCGCAGGA
                     GCAATGATCCACAGCAGGATCGGAAGAGTGGTGTTCGGAGCACGGGACGCCAAGACCG
                     GCGCAGCAGGCTCCCTGATGGATGTGCTGCACCACCCCGGCATGAACCACCGGGTGGA
                     GATCACAGAGGGAATCCTGGCAGACGAGTGCGCCGCCCTGCTGAGCGATTTCTTTAGA
                     ATGCGGAGACAGGAGATCAAGGCCCAGAAGAAGGCACAGAGCTCCACCGACTCTGGAG
                     GATCTAGCGGAGGATCCTCTGGAAGCGAGACACCAGGCACAAGCGAGTCCGCCACACC
                     AGAGAGCTCCGGCGGCTCCTCCGGAGGATCC"
                     /label="TCTGAAGTCGAGTTTAGCCACGAGTATTGGATGAGGCACGCACTGACCCT
                     GGCAAAGCGAGCATGGGATGAAAGAGAAGTCCCCGTGGGCGCCGTGCTGGTGCACAAC
                     AATAGAGTGATCGGAGAGGGATGGAACAGGCCAATCGGCCGCCACGACCCTACCGCAC
                     ACGCAGAGATCATGGCACTGAGGCAGGGAGGCCTGGTCATGCAGAATTACCGCCTGAT
                     CGATGCCACCCTGTATGTGACACTGGAGCCATGCGTGATGTGCGCAGGAGCAATGATC
                     CACAGCAGGATCGGAAGAGTGGTGTTCGGAGCACGGGACGCCAAGACCGGCGCAGCAG
                     GCTCCCTGATGGATGTGCTGCACCACCCCGGCATGAACCACCGGGTGGAGATCACAGA
                     GGGAATCCTGGCAGACGAGTGCGCCGCCCTGCTGAGCGATTTCTTTAGAATGCGGAGA
                     CAGGAGATCAAGGCCCAGAAGAAGGCACAGAGCTCCACCGACTCTGGAGGATCTAGCG
                     GAGGATCCTCTGGAAGCGAGACACCAGGCACAAGCGAGTCCGCCACACCAGAGAGCTC
                     CGGCGGCTCCTCCGGAGGATCC"
                     /note="Geneious type: Editing History Deletion"
     domain          466..963
                     /created_by="User"
                     /label="tadA(ABE7.10)"
     mutation        706..708
                     /created_by="User"
                     /label="V82G"
     misc_feature    706^707
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    708
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     964..1059
                     /Sequence="TCCGGAGGATCTAGCGGAGGCTCCTCTGGCTCTGAGACACCTGGCAC
                     AAGCGAGAGCGCAACACCTGAAAGCAGCGGGGGCAGCAGCGGGGGGTCA"
                     /Color="#999999"
                     /created_by="User"
                     /label="3GGS-XTEN-3GGS"
     primer_bind     1041..1085
                     /created_by="User"
                     /Sequence="GGGCAGCAGCGGGGGGTCAGACAAGAAGTACAGCATCGGCCTGGC"
                     /modified_by="User"
                     /label="oRZ592"
     primer_bind     complement(1041..1059)
                     /Sequence="TGACCCCCCGCTGCTGCCC"
                     /Hairpin_Tm="35.4"
                     /Tm="69.7"
                     /Self_Dimer_Tm="None"
                     /%GC="78.9"
                     /modified_by="User"
                     /label="oRZ590"
     primer_bind     complement(1043..1074)
                     /Sequence="GCTGTACTTCTTGTCTGACCCCCCGCTGCTGC"
                     /Hairpin_Tm="None"
                     /Tm="74.5"
                     /Self_Dimer_Tm="5.3"
                     /%GC="62.5"
                     /created_by="primer3"
                     /Color="#00ff00"
                     /label="oRZ591"
     primer_bind     1047..1083
                     /Sequence="CAGCGGGGGGTCAGACAAGAAGTACAGCATCGGCCTG"
                     /Color="#00ff00"
                     /created_by="User"
                     /label="oRZ593"
     domain          1060..5160
                     /created_by="User"
                     /Color="#034700"
                     /modified_by="User"
                     /label="nCas9_NG"
     source          <1060..>5160
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     mutation        1084..1086
                     /created_by="User"
                     /label="D10A"
     misc_feature    4388..5067
                     /Original_Bases="TGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAG
                     GACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGC
                     TGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCT
                     GCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTG
                     GAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACT
                     CCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCA
                     GAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGC
                     CACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGG
                     AACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAG
                     AGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGG
                     GATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATC
                     TGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACAC
                     C"
                     /label="TGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGAC
                     CCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGG
                     CCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGAT
                     CACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAG
                     GGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCG
                     AGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAA
                     CGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAG
                     AAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACA
                     AGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCT
                     GGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCC
                     ATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCC
                     CTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACC"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(5136..5184)
                     /Sequence="GGCGGTTCTTTTTGAGCCGCCAGAGTCACCTCCCAGCTGAGACAGGT
                     CG"
                     /created_by="User"
                     /label="oRZ596"
     primer_bind     complement(5139..5170)
                     /Sequence="AGCCGCCAGAGTCACCTCCCAGCTGAGACAGG"
                     /Hairpin_Tm="38.6"
                     /Tm="76.1"
                     /Self_Dimer_Tm="14.8"
                     /%GC="65.6"
                     /created_by="primer3"
                     /Color="#00ff00"
                     /label="oRZ597"
     primer_bind     5146..5182
                     /Sequence="CAGCTGGGAGGTGACTCTGGCGGCTCAAAAAGAACCG"
                     /Color="#00ff00"
                     /created_by="User"
                     /label="oRZ595"
     primer_bind     5161..5184
                     /Sequence="TCTGGCGGCTCAAAAAGAACCGCC"
                     /Hairpin_Tm="71.9"
                     /Tm="68.2"
                     /Self_Dimer_Tm="32.5"
                     /%GC="58.3"
                     /created_by="primer3"
                     /label="oRZ594"
     primer_bind     5173..5223
                     /Sequence="AAAAGAACCGCCGACGGCAGCGAATTCGAGCCCAAGAAGAAGAGGAA
                     AGTC"
                     /Color="#33bbff"
                     /created_by="User"
                     /label="bpNLS"
     misc_feature    5224..6498
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     domain          5224..5319
                     /Color="#cccccc"
                     /created_by="User"
                     /label="linker"
     source          <5224..>5319
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     region          <5320..5940
                     /created_by="User"
                     /Color="#ffbb33"
                     /modified_by="User"
                     /label="pmCDA1_NCBI: ABO15149.1  (R187W)"
     source          <5941..>6498
                     /organism="pCMV-BE3"
                     /mol_type="other DNA"
     source          5941..>6498
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="Genscript 2xUGI"
     primer_bind     5941..5970
                     /Sequence="AGCGGCGGGAGCGGCGGGAGCGGGGGGAGC"
                     /Hairpin_Tm="69.7"
                     /Tm="85.5"
                     /Self_Dimer_Tm="16.9"
                     /%GC="86.7"
                     /created_by="primer3"
                     /Color="#777777"
                     /modified_by="User"
                     /label="10AA linker 1"
     primer_bind     5971..6219
                     /Sequence="ACTAATCTGAGCGACATCATTGAGAAGGAGACTGGGAAACAGCTGGT
                     CATTCAGGAGTCCATCCTGATGCTGCCTGAGGAGGTGGAGGAAGTGATCGGCAACAAG
                     CCAGAGTCTGACATCCTGGTGCACACCGCCTACGACGAGTCCACAGATGAGAATGTGA
                     TGCTGCTGACCTCTGACGCCCCCGAGTATAAGCCTTGGGCCCTGGTCATCCAGGATTC
                     TAACGGCGAGAATAAGATCAAGATGCTG"
                     /Color="#ffff11"
                     /created_by="User"
                     /label="UGI No1 "
     primer_bind     6220..6249
                     /Sequence="AGCGGAGGATCCGGAGGATCTGGAGGCAGC"
                     /Hairpin_Tm="75.2"
                     /Tm="75.1"
                     /Self_Dimer_Tm="22.3"
                     /%GC="66.7"
                     /Color="#777777"
                     /modified_by="User"
                     /label="10AA linker"
     primer_bind     6250..6498
                     /Sequence="ACCAACCTGTCTGACATCATCGAGAAGGAGACAGGCAAGCAGCTGGT
                     CATCCAGGAGAGCATCCTGATGCTGCCCGAAGAAGTCGAAGAAGTGATCGGAAACAAG
                     CCTGAGAGCGATATCCTGGTCCATACCGCCTACGACGAGAGTACCGACGAAAATGTGA
                     TGCTGCTGACATCCGACGCCCCAGAGTATAAGCCCTGGGCTCTGGTCATCCAGGATTC
                     CAACGGAGAGAACAAAATCAAAATGCTG"
                     /Color="#ffff33"
                     /created_by="User"
                     /label="UGI No. 2"
     CDS             6508..6564
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding
                     separatepolypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /Color="#0033aa"
                     /modified_by="User"
                     /label="P2A"
     CDS             6565..7281
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
                     /Color="#11ff11"
                     /modified_by="User"
                     /label="EGFP"
     primer_bind     7218..7239
                     /note="EGFP, forward primer"
                     /label="EGFP-C"
     CDS             7294..7314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     primer_bind     7318..7321
                     /created_by="User"
                     /Sequence="CCGG"
                     /Color="#ff00ff"
                     /modified_by="User"
                     /label="cut BB"
     primer_bind     complement(7320..7355)
                     /Sequence="GCGGGTTTAAACTCAATGGTGATGGTGATGATGACC"
                     /Hairpin_Tm="31.9"
                     /Tm="70.2"
                     /Self_Dimer_Tm="6.9"
                     /%GC="47.2"
                     /modified_by="User"
                     /label="oRZ174"
     CDS             7323..7340
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /translation="HHHHHH"
                     /label="6xHis"
     primer_bind     complement(7363..7380)
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /label="BGH-rev"
     polyA_signal    7369..7593
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     primer_bind     complement(7664..7680)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     complement(7664..7680)
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     complement(7677..7699)
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     protein_bind    7688..7704
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7712..7742)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7757..7778
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     primer_bind     complement(7895..7912)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     rep_origin      complement(8066..8654)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(8146..8165)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(8825..9685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     9448..9467
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(9686..9790)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     complement(9869..9888)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     enhancer        10060..131
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg
      541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacggcac attcgagcct
      721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
      781 aggaacgcaa aaaccggcgc cgcaggctcc ctgatggacg tgctgcacta ccccggcatg
      841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      901 tatttctttc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccacc
      961 gactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
     1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag acaagaagta cagcatcggc
     1081 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     1141 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     1201 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     1261 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     1321 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     1381 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     1441 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     1501 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     1561 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     1621 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     1681 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     1741 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     1801 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     1861 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     1921 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     1981 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     2041 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     2101 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     2161 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     2221 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     2281 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     2341 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     2401 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     2461 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     2521 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     2581 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     2641 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     2701 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     2761 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     2821 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     2881 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     2941 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     3001 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     3061 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     3121 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     3181 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     3241 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     3301 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     3361 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     3421 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     3481 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     3541 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     3601 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     3661 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     3721 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     3781 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     3841 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     3901 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     3961 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     4021 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     4081 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     4141 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     4201 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     4261 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     4321 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     4381 tctatccggc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     4441 aagaagtacg gcggcttcgt gagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     4501 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     4561 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     4621 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     4681 ggccggaaga gaatgctggc ctctgcccgg ttcctgcaga agggaaacga actggccctg
     4741 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     4801 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     4861 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     4921 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     4981 atcatccacc tgtttaccct gaccaatctg ggagcccctc gggccttcaa gtactttgac
     5041 accaccatcg accggaaggt gtaccggagc accaaagagg tgctggacgc caccctgatc
     5101 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac
     5161 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     5221 gtctctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc
     5281 gccacaccag agagctccgg cggctcctcc ggaggatcca cagacgccga gtacgtgcgc
     5341 attcatgaga aactggatat ttacaccttc aagaagcagt tcttcaacaa caagaaatct
     5401 gtgtcacacc gctgctacgt gctgtttgag ttgaagcgaa ggggcgaaag aagggcttgc
     5461 ttttggggct atgccgtcaa caagccccaa agtggcaccg agagaggaat acacgctgag
     5521 atattcagta tccgaaaggt ggaagagtat cttcgggata atcctgggca gtttacgatc
     5581 aactggtatt ccagctggag tccttgcgct gattgtgccg agaaaattct ggaatggtat
     5641 aatcaggaac ttcggggaaa cgggcacaca ttgaaaatct gggcctgcaa gctgtactac
     5701 gagaagaatg cccggaacca gataggactc tggaatctga gggacaatgg tgtaggcctg
     5761 aacgtgatgg tttccgagca ctatcagtgt tgtcggaaga ttttcatcca aagctctcat
     5821 aaccagctca atgaaaaccg ctggttggag aaaacactga aacgtgcgga gaagtggaga
     5881 tccgagctga gcatcatgat ccaggtcaag attctgcata ccactaagtc tccagccgtt
     5941 agcggcggga gcggcgggag cggggggagc actaatctga gcgacatcat tgagaaggag
     6001 actgggaaac agctggtcat tcaggagtcc atcctgatgc tgcctgagga ggtggaggaa
     6061 gtgatcggca acaagccaga gtctgacatc ctggtgcaca ccgcctacga cgagtccaca
     6121 gatgagaatg tgatgctgct gacctctgac gcccccgagt ataagccttg ggccctggtc
     6181 atccaggatt ctaacggcga gaataagatc aagatgctga gcggaggatc cggaggatct
     6241 ggaggcagca ccaacctgtc tgacatcatc gagaaggaga caggcaagca gctggtcatc
     6301 caggagagca tcctgatgct gcccgaagaa gtcgaagaag tgatcggaaa caagcctgag
     6361 agcgatatcc tggtccatac cgcctacgac gagagtaccg acgaaaatgt gatgctgctg
     6421 acatccgacg ccccagagta taagccctgg gctctggtca tccaggattc caacggagag
     6481 aacaaaatca aaatgctggg aagcggagct actaacttca gcctgctgaa gcaggctgga
     6541 gacgtggagg agaaccctgg acctatggtg agcaagggcg aggagctgtt caccggggtg
     6601 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     6661 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6721 aagctgcccg tgccctggcc caccctcgtg accaccctga cctatggagt gcagtgcttc
     6781 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6841 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6901 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6961 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     7021 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     7081 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     7141 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     7201 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     7261 ggcatggacg agctgtacaa gtctggtggt tctcccaaga agaagaggaa agtctaaccg
     7321 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     7381 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7441 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7501 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7561 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7621 gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7681 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7741 aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacact cagtggaacg
     8701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9781 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat
     9841 ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc
     9901 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     9961 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
    10021 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
    10081 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    10141 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10201 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10261 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatc
//
LOCUS       pRZ5150_midi_CMVf_2019-11-16_B01.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...i0-b7ph4z2
VERSION     urn.local...i0-b7ph4z2
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..34
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         738..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ccgggtcggg gagtcttata gcagagctgg tttagtgaac cgtcagatcc gctagagatc
       61 cgcggccgct aatacgactc actataggga gagccgccac catgaaacgg acagccgacg
      121 gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc tgaggtggag ttttcccacg
      181 agtactggat gagacatgcc ctgaccctgg ccaagagggc acgcgatgag agggaggtgc
      241 ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca
      301 tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg
      361 tcatgcagaa ctacagactg attgacgcca ccctgtacgg cacattcgag ccttgcgtga
      421 tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaacg
      481 caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca ctaccccggc atgaatcacc
      541 gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg tgctatttct
      601 ttcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc accgactccg
      661 gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag agcgcaacac
      721 ctgaaagcag cgggggcagc agcggggggg tcagacaaga agtacagcat cggcctggcc
      781 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaagtg cccagcaaga
      841 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
      901 tgctgtcgac agcggcgaaa cagcgagcca cccggctgag agacgcagag agatacacca
      961 gacgaggacg atctgctatc tgcagagatc tcagcaccga
//
LOCUS       pRZ5150_midi_oRZ148__2019-11-19_B09.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...ls-b7ub84q
VERSION     urn.local...ls-b7ub84q
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..30
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         778..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ggtgcggtcg gtgttgccag caccttgatt tcttgctggg caccttgtac tcgtcggtga
       61 tcacggccca gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtctg
      121 accccccgct gctgcccccg ctgctttcag gtgttgcgct ctcgcttgtg ccaggtgtct
      181 cagagccaga ggagcctccg ctagatcctc cggagtcggt ggagctctgg gccttcttct
      241 gagcattgaa cacctgtcta ggcatccgaa agaaatagca cagcagggcg gcacattcat
      301 ctgccaggat tccctcggta atttcgacgc ggtgattcat gccggggtag tgcagcacgt
      361 ccatcaggga gcctgcggcg ccggtttttg cgttcctcac gccaaacacc acgcggccga
      421 tcctagagtg gatcatggcg ccggcgcaca tcacgcaagg ctcgaatgtg ccgtacaggg
      481 tggcgtcaat cagtctgtag ttctgcatga ccaggccgcc ctgtctcagg gccataattt
      541 cggcatgggc tgttgggtcg tgcaggccga tggctctgtt ccagccctcg ccgatcactc
      601 tattgttcag caccagcacg gctcccacag gcacctccct ctcatcgcgt gccctcttgg
      661 ccagggtcag ggcatgtctc atccagtact cgtgggaaaa ctccacctca gagactttcc
      721 gcttcttctt tggtgactcg aactcgcttc cgtcggctgt ccgtttcatg gtggcggctc
      781 tccctatagt gagtcgtatt agcggccgcg gatctctagc ggatctgacg gttcactaaa
      841 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcatgggg
      901 cggagttgtt acgacatttt ggaaagtccc gttgatttgg tgccaaacaa ctcccattga
      961 cgtcaatggg gtggagactg gaaatctccg tgagtcaacc
//
LOCUS       pRZ5150_midi_oRZ148_2019-11-16_B12.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...jo-b7ph50s
VERSION     urn.local...jo-b7ph50s
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..75
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         529..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 aaggggcggt cggtgtgcag caccttgatt tcttgctggg caccttgtac tcgtcggtga
       61 tcacggccca gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtctg
      121 accccccgct gctgcccccg ctgctttcag gtgttgcgct ctcgcttgtg ccaggtgtct
      181 cagagccaga ggagcctccg ctagatcctc cggagtcggt ggagctctgg gccttcttct
      241 gagcattgaa cacctgtcta ggcatccgaa agaaatagca cagcagggcg gcacattcat
      301 ctgccaggat tccctcggta atttcgacgc ggtgattcat gccggggtag tgcagcacgt
      361 ccatcaggga gcctgcggcg ccggtttttg cgttcctcac gccaaacacc acgcggccga
      421 tcctagagtg gatcatggcg ccggcgcaca tcacgcaagg ctcgaatgtg ccgtacaggg
      481 tggcgtcaat cagtctgtag ttctgcatga ccaggccgcc ctgtctcagg gccataattt
      541 cggcatgggc tgttgggtcg tgcaggccga tggctctgtt ccagccctcg ccgatcactc
      601 tattgttcag caccagcacg gctcccacag gcacctccct ctcatcgcgt gccctcttgg
      661 ccagggtcag gcatgtctca tccagtactc gtgggaaaac tccacctcag agactttccg
      721 cttcttcttt ggtgactcga actcgcttcc gtcggctgtc cgtttcatgg tggcggctct
      781 ccctatagtg agtcgtatta gcggccgcgg atctctagcg gatctgacgg ttcactaaac
      841 cagctctgct tatatagact cccaccgtac acgcctacgc ccattgcgtc atggggcgga
      901 gtgtacgaca ttttgaaagt ccgtgatttg tgcaaaccaa ctccatgacg tcatggggtg
      961 agacctgcaa ttccgttgag tcaaccgtat cagtccattg
//
LOCUS       pRZ5150_midi_oJUL1084__2019-11-19_A09.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...lm-b7ub84l
VERSION     urn.local...lm-b7ub84l
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..32
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         673..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ttgtcattta gggctatggc gtgaggacgc aaaaaccggc gccgcaggct ccctgatgga
       61 cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga
      121 tgaatgtgcc gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca
      181 gaagaaggcc cagagctcca ccgactccgg aggatctagc ggaggctcct ctggctctga
      241 gacacctggc acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc
      301 agacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
      361 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      421 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      481 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      541 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
      601 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
      661 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
      721 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
      781 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
      841 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaccccatc
      901 acgccagcgg cgtggacgcc aaggccatcc tgtctgcaga ctgagcagag cagacggctg
      961 gaaatctgat cgcccagctg ccggcgagag aaaatggcct
//
LOCUS       pRZ5150_midi_oJUL1085_2019-11-16_B03.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...i6-b7ph4z8
VERSION     urn.local...i6-b7ph4z8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..45
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         741..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ggggaattcg cacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
       61 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
      121 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
      181 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
      241 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
      301 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
      361 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa
      421 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
      481 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
      541 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
      601 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
      661 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
      721 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
      781 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
      841 cggcggagcc agccaggaag agttctacaa gttcatcaag ctcatcctgg aaagatggac
      901 ggcaccgagg actgctcgtg agctgaacag agaggacctg ctgcgagcag cggatctcga
      961 cacggcagca tccctcacag attcatcttg gggagagctg
//
LOCUS       pRZ5150_midi_oJUL1086_2019-11-16_B04.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...ic-b7ph4ze
VERSION     urn.local...ic-b7ph4ze
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..40
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         670..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 tgggcacggc gacagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
       61 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
      121 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
      181 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
      241 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
      301 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
      361 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
      421 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
      481 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
      541 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
      601 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
      661 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
      721 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
      781 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
      841 gtgaagcagc tgaaagagga ctacttcaga aaatcgagtg cttcgactcc gtggaaatct
      901 cggcgtgaag atcgggttca acgcctccct gggcacatac aacgatctgc tgacattatc
      961 aaggaccaga cctctgacat gagaaacgag acatctggag
//
LOCUS       pRZ5150_midi_oJUL1087_2019-11-16_B05.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...ii-b7ph4zk
VERSION     urn.local...ii-b7ph4zk
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..36
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         775..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 tgcatttatg gtacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
       61 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
      121 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
      181 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
      241 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
      301 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
      361 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
      421 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
      481 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
      541 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
      601 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
      661 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
      721 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
      781 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
      841 aggcatcctg cagacagtga aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
      901 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
      961 gacagccgcg agaagaatga ggcggatcga gagggcatca
//
LOCUS       pRZ5150_midi_oJUL1088_2019-11-16_B06.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...io-b7ph4zr
VERSION     urn.local...io-b7ph4zr
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..32
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         701..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 aacggctgcc tgacctgacc tgtttgagga cagagagatg atcgaggaac ggctgaaaac
       61 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg
      121 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac
      181 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca
      241 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga
      301 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct
      361 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa
      421 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg
      481 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga
      541 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa
      601 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt
      661 ggaccatatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac
      721 cagaagcgac aagaaccggg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
      781 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaagttcga
      841 caatctgacc aaaggccgag agaggcgggc ctgagcgaac tgggataagg ccggcttcat
      901 caagagacag ctggtgcaaa cccgggcaga tcacaagcac gtggcacaga tctgactccc
      961 ggatgacact aagtacgacg agaatgacaa gctgaatcgg
//
LOCUS       pRZ5150_midi_oJUL1089_2019-11-16_B07.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...iu-b7ph4zx
VERSION     urn.local...iu-b7ph4zx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..43
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         706..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ccgattcatc tgcagatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
       61 tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg
      121 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
      181 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
      241 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
      301 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
      361 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
      421 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
      481 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
      541 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
      601 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
      661 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
      721 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
      781 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
      841 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aagagtctat ccgggcccag
      901 aggacagcga tagctgatcg ccagaaagac gactggacct agagtacggc ggctcgtgag
      961 cccaacgtgt ctatttctgg tgctggtgtg caaagtgaaa
//
LOCUS       pRZ5150_midi_oJUL1090_2019-11-16_B08.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...j0-b7ph504
VERSION     urn.local...j0-b7ph504
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..76
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         701..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 aggcatgctc tcacgtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
       61 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
      121 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
      181 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
      241 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
      301 tccggcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
      361 agtacggcgg cttcgtgagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
      421 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
      481 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
      541 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
      601 ggaagagaat gctggcctct gcccggttcc tgcagaaggg aaacgaactg gccctgccct
      661 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
      721 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
      781 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
      841 tgctgtccgc ctacacaagc accgggataa gcccatcaga gagcaggccg agatatcatc
      901 cacctgttac cctgaccatc tgggagcccc tcgggcttca gtacttgaca caacatcgac
      961 cggaggtgta cggagcacaa gaggtgctgg acgccacctg
//
LOCUS       pRZ5150_midi_oJUL1091_2019-11-16_B09.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...j6-b7ph50a
VERSION     urn.local...j6-b7ph50a
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..15
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         682..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 cggactgatc atcagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
       61 atgctggcct ctgcccggtt cctgcagaag ggaaacgaac tggccctgcc ctccaaatat
      121 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
      181 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
      241 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
      301 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
      361 tttaccctga ccaatctggg agcccctcgg gccttcaagt actttgacac caccatcgac
      421 cggaaggtgt accggagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
      481 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggtgactc tggcggctca
      541 aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctctggagga
      601 tctagcggag gatcctctgg aagcgagaca ccaggcacaa gcgagtccgc cacaccagag
      661 agctccggcg gctcctccgg aggatccaca gacgccgagt acgtgcgcat tcatgagaaa
      721 ctggatattt acaccttcaa gaagcagttc ttcaacaaca agaaatctgt gtcacaccgc
      781 tgctacgtgc tgtttgagtt gaagcgaaag gggcgaaaga aggcttgctt ttggggctat
      841 gcccgtcaac aagctctcaa agtgcaccga gagaggaata cacgctgaga tattcagtat
      901 ccgaaaggtg aagagtatct tcgggatatc cttggcaggt tacgatcact gctattcagc
      961 ctgagttctg cgctgatgtg cgagaaattc tgcattgtat
//
LOCUS       pRZ5150_midi_oJUL1094_2019-11-16_B10.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...jc-b7ph50g
VERSION     urn.local...jc-b7ph50g
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..30
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         722..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 cgacgtcgag tatctggcgg ctcaaaagaa ccgccgacgg cagcgaattc gagcccaaga
       61 agaagaggaa agtctctgga ggatctagcg gaggatcctc tggaagcgag acaccaggca
      121 caagcgagtc cgccacacca gagagctccg gcggctcctc cggaggatcc acagacgccg
      181 agtacgtgcg cattcatgag aaactggata tttacacctt caagaagcag ttcttcaaca
      241 acaagaaatc tgtgtcacac cgctgctacg tgctgtttga gttgaagcga aggggcgaaa
      301 gaagggcttg cttttggggc tatgccgtca acaagcccca aagtggcacc gagagaggaa
      361 tacacgctga gatattcagt atccgaaagg tggaagagta tcttcgggat aatcctgggc
      421 agtttacgat caactggtat tccagctgga gtccttgcgc tgattgtgcc gagaaaattc
      481 tggaatggta taatcaggaa cttcggggaa acgggcacac attgaaaatc tgggcctgca
      541 agctgtacta cgagaagaat gcccggaacc agataggact ctggaatctg agggacaatg
      601 gtgtaggcct gaacgtgatg gtttccgagc actatcagtg ttgtcggaag attttcatcc
      661 aaagctctca taaccagctc aatgaaaacc gctggttgga gaaaacactg aaacgtgcgg
      721 agaagtggag atccgagctg agcatcatga tccaggtcaa gattctgcat accactaagt
      781 ctccagccgt tagcggcggg agcggcggga gcggggggag cactaatctg agcgacatca
      841 ttgagaagga gactgggaaa cagctggtca ttcaggagtc catcctgatg ctgcctgagg
      901 aggtggaggg agtgatcggg cacagccaga gtctgacatc ctgtgcacat cgcctacgac
      961 gagtccacag atgagatgtg atgctgctga ctctgacgct
//
LOCUS       pRZ5150_midi_oRZ218__2019-11-19_C09.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...ly-b7ub84w
VERSION     urn.local...ly-b7ub84w
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..27
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         637..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 atgcatggtg tcttgcgctg attgtgccga gaaaattctg gaatggtata atcaggaact
       61 tcggggaaac gggcacacat tgaaaatctg ggcctgcaag ctgtactacg agaagaatgc
      121 ccggaaccag ataggactct ggaatctgag ggacaatggt gtaggcctga acgtgatggt
      181 ttccgagcac tatcagtgtt gtcggaagat tttcatccaa agctctcata accagctcaa
      241 tgaaaaccgc tggttggaga aaacactgaa acgtgcggag aagtggagat ccgagctgag
      301 catcatgatc caggtcaaga ttctgcatac cactaagtct ccagccgtta gcggcgggag
      361 cggcgggagc ggggggagca ctaatctgag cgacatcatt gagaaggaga ctgggaaaca
      421 gctggtcatt caggagtcca tcctgatgct gcctgaggag gtggaggaag tgatcggcaa
      481 caagccagag tctgacatcc tggtgcacac cgcctacgac gagtccacag atgagaatgt
      541 gatgctgctg acctctgacg cccccgagta taagccttgg gccctggtca tccaggattc
      601 taacggcgag aataagatca agatgctgag cggaggatcc ggaggatctg gaggcagcac
      661 caacctgtct gacatcatcg agaaggagac aggcaagcag ctggtcatcc aggagagcat
      721 cctgatgctg cccgaagaag tcgaagaagt gatcggaaac aagcctgaga gcgatatcct
      781 ggtccatacc gcctacgacg agagtaccga cgaaaatgtg atgctgctga catccgacgc
      841 ccagagtata agccctgggc tctggtcatc caggattcca cggagagaca aatcaaatgc
      901 tgggaagcgg agctactaac ttcagctgct gagcaggtgg agacgtggag gagaacctgg
      961 actatgtgag caggcgagga ctttcaccgg tggtgccatt
//
LOCUS       pRZ5150_midi_oRZ608__2019-11-20_A01.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...r1-b8f37gn
VERSION     urn.local...r1-b8f37gn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..29
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         616..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gtactactct cacgtctcag cctgcttcag caggctgaag ttagtagctc cgcttcccag
       61 cattttgatt ttgttctctc cgttggaatc ctggatgacc agagcccagg gcttatactc
      121 tggggcgtcg gatgtcagca gcatcacatt ttcgtcggta ctctcgtcgt aggcggtatg
      181 gaccaggata tcgctctcag gcttgtttcc gatcacttct tcgacttctt cgggcagcat
      241 caggatgctc tcctggatga ccagctgctt gcctgtctcc ttctcgatga tgtcagacag
      301 gttggtgctg cctccagatc ctccggatcc tccgctcagc atcttgatct tattctcgcc
      361 gttagaatcc tggatgacca gggcccaagg cttatactcg ggggcgtcag aggtcagcag
      421 catcacattc tcatctgtgg actcgtcgta ggcggtgtgc accaggatgt cagactctgg
      481 cttgttgccg atcacttcct ccacctcctc aggcagcatc aggatggact cctgaatgac
      541 cagctgtttc ccagtctcct tctcaatgat gtcgctcaga ttagtgctcc ccccgctccc
      601 gccgctcccg ccgctaacgg ctggagactt agtggtatgc agaatcttga cctggatcat
      661 gatgctcagc tcggatctcc acttctccgc acgtttcagt gttctctcca accagcggtt
      721 ttcattgagc tggttatgag agctttggat gaaaatcttc cgacacactg atagtgctcg
      781 gaaaccatca cgttcagcct acaccattgt ccctcagatt ccagagtcct atctggttcc
      841 gggcatctct cgtagtacag ctgcagccca gattttcatg tgtgcccgtt tcccgagtct
      901 gatataccat tccagatttc tcgcacatca gcgcagactc agctgataca gtgatcgtaa
      961 ctgccagaat tatccgagat ccctccactc gatctgaaat
//
LOCUS       pRZ5150_midi_BGHrev_2019-11-16_B02.ab1        1000 bp    DNA     linear   UNA 14-FEB-2020
DEFINITION  .
ACCESSION   urn.local...hu-b7ph4xk
VERSION     urn.local...hu-b7ph4xk
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..34
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         830..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 cggcatggct actcatggtg atggtgatga tgaccggtta gactttcctc ttcttcttgg
       61 gagaaccacc agacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      121 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      181 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      241 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      301 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      361 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      421 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      481 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      541 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcactcca taggtcaggg
      601 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      661 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      721 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      781 tgctcaccat aggtccaggg ttctcctcca cgtctccagc ctgcttcagc caggctgaaa
      841 gttagtagct ccgcttccca gcatttttga ttttgttctc tccgtggaaa tcctggatga
      901 ccagagcgca ggctatactc tgggggcgtc ggatgtcagc agcatcacat ttcgtcgtac
      961 tctccgtcgt agcgaatgac agatatcgct cctcaggctg
//
LOCUS       pRZ5150_midi_BGHrev__2019-11-19_D09.ab1        1000 bp    DNA     linear   UNA 23-NOV-2019
DEFINITION  .
ACCESSION   urn.local...lg-b7ub848
VERSION     urn.local...lg-b7ub848
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..29
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         645..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ggcgggctac tcatggtgat ggtgatgatg accggttaga ctttcctctt cttcttggga
       61 gaaccaccag acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      121 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      181 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      241 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      301 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      361 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      421 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      481 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      541 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcactccata ggtcagggtg
      601 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      661 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
      721 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
      781 ctcaccatag gtccagggtt ctcctccacg tctccagcct gcttcagcag ctgaagttag
      841 tagctcgctt cccagcattt tgatttgtct ctccgttgga atcctgatga cagagcccag
      901 gctatactct ggggcgtcga tgtcagcagc atcacatttt cgtcgtactc ctcgtcgtag
      961 gcggatggac agatatcgct ctcaagcttg ttcgatccct
//
