LOCUS       pRZ5137_=_SPACE_VRQR       10308 bp ds-DNA     circular SYN 14-FEB-2020
DEFINITION  pCMV-bpNLS-TadA*(V82G)-nCas9_VRQR-bpNLS-pmCDA1(R187W)-UGI-UGI-P2A-E
            GFP.
ACCESSION
VERSION     .
KEYWORDS    pCMV_ABEmax_P2A_GFP.
SOURCE      
  ORGANISM  .
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Koblan, LW, Doman, JL, Wilson, C, Levy, JM, Tay, T, Newby, GA,
            Maianti, JP, Raguram, A, Liu and DR
  TITLE     Improving cytidine and adenine base editors by expression
            optimization and ancestral reconstruction.
  JOURNAL   Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
  PUBMED    29813047
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     286..306
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     primer_bind     343..368
                     /Sequence="TGAACCGTCAGATCCGCTAGAGATCC"
                     /Hairpin_Tm="None"
                     /Tm="65.5"
                     /Self_Dimer_Tm="15.8"
                     /%GC="53.8"
                     /modified_by="User"
                     /label="oRZ176"
     primer_bind     371..374
                     /Sequence="GGCC"
                     /Color="#ff33ff"
                     /modified_by="User"
                     /label="cut BB"
     promoter        377..395
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     377..396
                     /note="T7 promoter, forward primer"
                     /label="T7"
     motif           400..411
                     /created_by="User"
                     /label="KOZAC"
     primer_bind     412..465
                     /created_by="User"
                     /Sequence="AAACGGACAGCCGACGGAAGCGAGTTCGAGTCACCAAAGAAGAAGCG
                     GAAAGTC"
                     /Color="#1199ff"
                     /modified_by="User"
                     /label="NLS"
     misc_feature    465^466
                     /Original_Bases="TCTGAAGTCGAGTTTAGCCACGAGTATTGGATGAGGCACGC
                     ACTGACCCTGGCAAAGCGAGCATGGGATGAAAGAGAAGTCCCCGTGGGCGCCGTGCTG
                     GTGCACAACAATAGAGTGATCGGAGAGGGATGGAACAGGCCAATCGGCCGCCACGACC
                     CTACCGCACACGCAGAGATCATGGCACTGAGGCAGGGAGGCCTGGTCATGCAGAATTA
                     CCGCCTGATCGATGCCACCCTGTATGTGACACTGGAGCCATGCGTGATGTGCGCAGGA
                     GCAATGATCCACAGCAGGATCGGAAGAGTGGTGTTCGGAGCACGGGACGCCAAGACCG
                     GCGCAGCAGGCTCCCTGATGGATGTGCTGCACCACCCCGGCATGAACCACCGGGTGGA
                     GATCACAGAGGGAATCCTGGCAGACGAGTGCGCCGCCCTGCTGAGCGATTTCTTTAGA
                     ATGCGGAGACAGGAGATCAAGGCCCAGAAGAAGGCACAGAGCTCCACCGACTCTGGAG
                     GATCTAGCGGAGGATCCTCTGGAAGCGAGACACCAGGCACAAGCGAGTCCGCCACACC
                     AGAGAGCTCCGGCGGCTCCTCCGGAGGATCC"
                     /label="TCTGAAGTCGAGTTTAGCCACGAGTATTGGATGAGGCACGCACTGACCCT
                     GGCAAAGCGAGCATGGGATGAAAGAGAAGTCCCCGTGGGCGCCGTGCTGGTGCACAAC
                     AATAGAGTGATCGGAGAGGGATGGAACAGGCCAATCGGCCGCCACGACCCTACCGCAC
                     ACGCAGAGATCATGGCACTGAGGCAGGGAGGCCTGGTCATGCAGAATTACCGCCTGAT
                     CGATGCCACCCTGTATGTGACACTGGAGCCATGCGTGATGTGCGCAGGAGCAATGATC
                     CACAGCAGGATCGGAAGAGTGGTGTTCGGAGCACGGGACGCCAAGACCGGCGCAGCAG
                     GCTCCCTGATGGATGTGCTGCACCACCCCGGCATGAACCACCGGGTGGAGATCACAGA
                     GGGAATCCTGGCAGACGAGTGCGCCGCCCTGCTGAGCGATTTCTTTAGAATGCGGAGA
                     CAGGAGATCAAGGCCCAGAAGAAGGCACAGAGCTCCACCGACTCTGGAGGATCTAGCG
                     GAGGATCCTCTGGAAGCGAGACACCAGGCACAAGCGAGTCCGCCACACCAGAGAGCTC
                     CGGCGGCTCCTCCGGAGGATCC"
                     /note="Geneious type: Editing History Deletion"
     domain          466..963
                     /created_by="User"
                     /label="tadA(ABE7.10)"
     mutation        706..708
                     /created_by="User"
                     /label="V82G"
     misc_feature    706^707
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    708
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     964..1059
                     /Sequence="TCCGGAGGATCTAGCGGAGGCTCCTCTGGCTCTGAGACACCTGGCAC
                     AAGCGAGAGCGCAACACCTGAAAGCAGCGGGGGCAGCAGCGGGGGGTCA"
                     /Color="#999999"
                     /created_by="User"
                     /label="3GGS-XTEN-3GGS"
     primer_bind     1041..1085
                     /Sequence="GGGCAGCAGCGGGGGGTCAGACAAGAAGTACAGCATCGGCCTGGC"
                     /created_by="User"
                     /label="oRZ592"
     primer_bind     complement(1041..1059)
                     /Sequence="TGACCCCCCGCTGCTGCCC"
                     /Hairpin_Tm="35.4"
                     /Tm="69.7"
                     /Self_Dimer_Tm="None"
                     /%GC="78.9"
                     /modified_by="User"
                     /label="oRZ590"
     primer_bind     complement(1043..1074)
                     /Sequence="GCTGTACTTCTTGTCTGACCCCCCGCTGCTGC"
                     /Hairpin_Tm="None"
                     /Tm="74.5"
                     /Self_Dimer_Tm="5.3"
                     /%GC="62.5"
                     /created_by="primer3"
                     /Color="#00ff00"
                     /label="oRZ591"
     primer_bind     1047..1083
                     /Sequence="CAGCGGGGGGTCAGACAAGAAGTACAGCATCGGCCTG"
                     /Color="#00ff00"
                     /created_by="User"
                     /label="oRZ593"
     source          join(<1060..>5160,<1060..>5160)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             <1060..5160
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /Original_Translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQ
                     SGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPA
                     IKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKE
                     LGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLK
                     DDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG
                     GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS
                     DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
                     AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
                     ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPT
                     VAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
                     KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
                     KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL
                     FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /modified_by="hstutzman"
                     /Color="#33bbff"
                     /modified_by="User"
                     /label="nSpCas9-VRQR (FZ human codon optimized)"
     misc_difference 1084..1086
                     /created_by="hstutzman"
                     /modified_by="hstutzman"
                     /label="D10A (GAC -> GCC)"
     primer_bind     1188..1210
                     /Sequence="GAAGAACCTGATCGGAGCCCTGC"
                     /Transferred_From="oBK7322"
                     /Transferred_Similarity="100.00%"
                     /label="oBK7322"
     primer_bind     1870..1891
                     /Sequence="GACGACGACCTGGACAACCTGC"
                     /Transferred_From="oBK7323"
                     /Transferred_Similarity="100.00%"
                     /label="oBK7323"
     primer_bind     2556..2577
                     /Sequence="GAACCTGCCCAACGAGAAGGTG"
                     /Transferred_From="oBK7324"
                     /Transferred_Similarity="100.00%"
                     /label="oBK7324"
     primer_bind     3252..3277
                     /Sequence="CATTAAGAAGGGCATCCTGCAGACAG"
                     /Transferred_From="oBK7325"
                     /Transferred_Similarity="100.00%"
                     /label="oBK7325"
     misc_difference 3574..3576
                     /created_by="hstutzman"
                     /modified_by="hstutzman"
                     /label="H840 (CAT)"
     primer_bind     3921..3945
                     /Sequence="GATCACCCTGAAGTCCAAGCTGGTG"
                     /Transferred_From="oBK7326"
                     /Transferred_Similarity="100.00%"
                     /label="oBK7326"
     misc_difference 4459
                     /created_by="hstutzman"
                     /modified_by="hstutzman"
                     /label="D1135V (GAC -> GTG)"
     misc_feature    4460..5157
                     /Original_Bases="ACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAA
                     GTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCA
                     TCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTA
                     CAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTG
                     GAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAAC
                     TGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCT
                     GAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCAC
                     TACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCG
                     ACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAG
                     AGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCC
                     GCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGG
                     TGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGA
                     CCTGTCTCAGCTGGGAGGT"
                     /label="ACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAG
                     GGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAA
                     GAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGT
                     GAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGC
                     CGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGC
                     CCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTC
                     CCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGAC
                     GAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATC
                     TGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGC
                     CGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAG
                     TACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACG
                     CCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCA
                     GCTGGGAGGT"
                     /note="Geneious type: Editing History Replacement"
     misc_difference 4462..4464
                     /created_by="hstutzman"
                     /label="S1136 (AGC)"
     primer_bind     4602..4626
                     /Sequence="GGAAGCCAAGGGCTACAAAGAAGTG"
                     /Transferred_From="oBK7327"
                     /Transferred_Similarity="100.00%"
                     /label="oBK7327"
     misc_difference 4708..4710
                     /created_by="hstutzman"
                     /modified_by="hstutzman"
                     /label="G1218R (GGC -> AGA)"
     misc_difference 4711..4713
                     /created_by="hstutzman"
                     /label="E1219 (GAA)"
     misc_difference 5053..5055
                     /created_by="hstutzman"
                     /label="R1333 (CGG)"
     misc_difference 5061
                     /created_by="hstutzman"
                     /modified_by="hstutzman"
                     /label="R1335Q (AGG-> CAG)"
     misc_difference 5065
                     /created_by="hstutzman"
                     /modified_by="hstutzman"
                     /label="T1337R (ACC->AGA)"
     primer_bind     complement(5137..5184)
                     /created_by="User"
                     /Sequence="GGCGGTTCTTTTTGAGCCGCCAGAGTCGCCTCCCAGCTGAGACAGGT
                     C"
                     /modified_by="User"
                     /label="oRZ598"
     primer_bind     complement(5140..5171)
                     /Sequence="GAGCCGCCAGAGTCGCCTCCCAGCTGAGACAG"
                     /Hairpin_Tm="38.6"
                     /Tm="76.7"
                     /Self_Dimer_Tm="7.4"
                     /%GC="68.8"
                     /created_by="primer3"
                     /Color="#00ff00"
                     /label="oRZ599"
     primer_bind     5150..5181
                     /Sequence="TGGGAGGCGACTCTGGCGGCTCAAAAAGAACC"
                     /Hairpin_Tm="56.3"
                     /Tm="74.4"
                     /Self_Dimer_Tm="22.5"
                     /%GC="59.4"
                     /created_by="primer3"
                     /Color="#00ff00"
                     /modified_by="User"
                     /label="oRZ600"
     primer_bind     5161..5184
                     /Sequence="TCTGGCGGCTCAAAAAGAACCGCC"
                     /Hairpin_Tm="71.9"
                     /Tm="68.2"
                     /Self_Dimer_Tm="32.5"
                     /%GC="58.3"
                     /created_by="primer3"
                     /label="oRZ594"
     primer_bind     5173..5223
                     /Sequence="AAAAGAACCGCCGACGGCAGCGAATTCGAGCCCAAGAAGAAGAGGAA
                     AGTC"
                     /Color="#33bbff"
                     /created_by="User"
                     /label="bpNLS"
     misc_feature    5224..6498
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     domain          5224..5319
                     /Color="#cccccc"
                     /created_by="User"
                     /label="linker"
     source          <5224..>5319
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     region          <5320..5940
                     /created_by="User"
                     /Color="#ffbb33"
                     /modified_by="User"
                     /label="pmCDA1_NCBI: ABO15149.1  (R187W)"
     source          <5941..>6498
                     /organism="pCMV-BE3"
                     /mol_type="other DNA"
     source          5941..>6498
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="Genscript 2xUGI"
     primer_bind     5941..5970
                     /Sequence="AGCGGCGGGAGCGGCGGGAGCGGGGGGAGC"
                     /Hairpin_Tm="69.7"
                     /Tm="85.5"
                     /Self_Dimer_Tm="16.9"
                     /%GC="86.7"
                     /created_by="primer3"
                     /Color="#777777"
                     /modified_by="User"
                     /label="10AA linker 1"
     primer_bind     5971..6219
                     /Sequence="ACTAATCTGAGCGACATCATTGAGAAGGAGACTGGGAAACAGCTGGT
                     CATTCAGGAGTCCATCCTGATGCTGCCTGAGGAGGTGGAGGAAGTGATCGGCAACAAG
                     CCAGAGTCTGACATCCTGGTGCACACCGCCTACGACGAGTCCACAGATGAGAATGTGA
                     TGCTGCTGACCTCTGACGCCCCCGAGTATAAGCCTTGGGCCCTGGTCATCCAGGATTC
                     TAACGGCGAGAATAAGATCAAGATGCTG"
                     /Color="#ffff11"
                     /created_by="User"
                     /label="UGI No1 "
     primer_bind     6220..6249
                     /Sequence="AGCGGAGGATCCGGAGGATCTGGAGGCAGC"
                     /Hairpin_Tm="75.2"
                     /Tm="75.1"
                     /Self_Dimer_Tm="22.3"
                     /%GC="66.7"
                     /Color="#777777"
                     /modified_by="User"
                     /label="10AA linker"
     primer_bind     6250..6498
                     /Sequence="ACCAACCTGTCTGACATCATCGAGAAGGAGACAGGCAAGCAGCTGGT
                     CATCCAGGAGAGCATCCTGATGCTGCCCGAAGAAGTCGAAGAAGTGATCGGAAACAAG
                     CCTGAGAGCGATATCCTGGTCCATACCGCCTACGACGAGAGTACCGACGAAAATGTGA
                     TGCTGCTGACATCCGACGCCCCAGAGTATAAGCCCTGGGCTCTGGTCATCCAGGATTC
                     CAACGGAGAGAACAAAATCAAAATGCTG"
                     /Color="#ffff33"
                     /created_by="User"
                     /label="UGI No. 2"
     CDS             6508..6564
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding
                     separatepolypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /Color="#0033aa"
                     /modified_by="User"
                     /label="P2A"
     CDS             6565..7281
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
                     /Color="#11ff11"
                     /modified_by="User"
                     /label="EGFP"
     primer_bind     7218..7239
                     /note="EGFP, forward primer"
                     /label="EGFP-C"
     CDS             7294..7314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     primer_bind     7318..7321
                     /created_by="User"
                     /Sequence="CCGG"
                     /Color="#ff00ff"
                     /modified_by="User"
                     /label="cut BB"
     primer_bind     complement(7320..7355)
                     /Sequence="GCGGGTTTAAACTCAATGGTGATGGTGATGATGACC"
                     /Hairpin_Tm="31.9"
                     /Tm="70.2"
                     /Self_Dimer_Tm="6.9"
                     /%GC="47.2"
                     /modified_by="User"
                     /label="oRZ174"
     CDS             7323..7340
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /translation="HHHHHH"
                     /label="6xHis"
     primer_bind     complement(7363..7380)
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /label="BGH-rev"
     polyA_signal    7369..7593
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     primer_bind     complement(7664..7680)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     complement(7664..7680)
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     complement(7677..7699)
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     protein_bind    7688..7704
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7712..7742)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7757..7778
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     primer_bind     complement(7895..7912)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     rep_origin      complement(8066..8654)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(8146..8165)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(8825..9685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     9448..9467
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(9686..9790)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     complement(9869..9888)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     enhancer        10060..131
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg
      541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacggcac attcgagcct
      721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
      781 aggaacgcaa aaaccggcgc cgcaggctcc ctgatggacg tgctgcacta ccccggcatg
      841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      901 tatttctttc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccacc
      961 gactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
     1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag acaagaagta cagcatcggc
     1081 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     1141 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     1201 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     1261 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     1321 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     1381 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     1441 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     1501 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     1561 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     1621 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     1681 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     1741 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     1801 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     1861 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     1921 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     1981 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     2041 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     2101 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     2161 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     2221 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     2281 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     2341 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     2401 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     2461 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     2521 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     2581 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     2641 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     2701 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     2761 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     2821 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     2881 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     2941 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     3001 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     3061 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     3121 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     3181 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     3241 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     3301 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     3361 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     3421 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     3481 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     3541 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     3601 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     3661 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     3721 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     3781 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     3841 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     3901 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     3961 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     4021 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     4081 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     4141 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     4201 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     4261 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     4321 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     4381 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     4441 aagaagtacg gcggcttcgt gagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     4501 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     4561 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     4621 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     4681 ggccggaaga gaatgctggc ctctgccaga gaactgcaga agggaaacga actggccctg
     4741 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     4801 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     4861 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     4921 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     4981 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     5041 accaccatcg accggaagca gtacagaagc accaaagagg tgctggacgc caccctgatc
     5101 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
     5161 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     5221 gtctctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc
     5281 gccacaccag agagctccgg cggctcctcc ggaggatcca cagacgccga gtacgtgcgc
     5341 attcatgaga aactggatat ttacaccttc aagaagcagt tcttcaacaa caagaaatct
     5401 gtgtcacacc gctgctacgt gctgtttgag ttgaagcgaa ggggcgaaag aagggcttgc
     5461 ttttggggct atgccgtcaa caagccccaa agtggcaccg agagaggaat acacgctgag
     5521 atattcagta tccgaaaggt ggaagagtat cttcgggata atcctgggca gtttacgatc
     5581 aactggtatt ccagctggag tccttgcgct gattgtgccg agaaaattct ggaatggtat
     5641 aatcaggaac ttcggggaaa cgggcacaca ttgaaaatct gggcctgcaa gctgtactac
     5701 gagaagaatg cccggaacca gataggactc tggaatctga gggacaatgg tgtaggcctg
     5761 aacgtgatgg tttccgagca ctatcagtgt tgtcggaaga ttttcatcca aagctctcat
     5821 aaccagctca atgaaaaccg ctggttggag aaaacactga aacgtgcgga gaagtggaga
     5881 tccgagctga gcatcatgat ccaggtcaag attctgcata ccactaagtc tccagccgtt
     5941 agcggcggga gcggcgggag cggggggagc actaatctga gcgacatcat tgagaaggag
     6001 actgggaaac agctggtcat tcaggagtcc atcctgatgc tgcctgagga ggtggaggaa
     6061 gtgatcggca acaagccaga gtctgacatc ctggtgcaca ccgcctacga cgagtccaca
     6121 gatgagaatg tgatgctgct gacctctgac gcccccgagt ataagccttg ggccctggtc
     6181 atccaggatt ctaacggcga gaataagatc aagatgctga gcggaggatc cggaggatct
     6241 ggaggcagca ccaacctgtc tgacatcatc gagaaggaga caggcaagca gctggtcatc
     6301 caggagagca tcctgatgct gcccgaagaa gtcgaagaag tgatcggaaa caagcctgag
     6361 agcgatatcc tggtccatac cgcctacgac gagagtaccg acgaaaatgt gatgctgctg
     6421 acatccgacg ccccagagta taagccctgg gctctggtca tccaggattc caacggagag
     6481 aacaaaatca aaatgctggg aagcggagct actaacttca gcctgctgaa gcaggctgga
     6541 gacgtggagg agaaccctgg acctatggtg agcaagggcg aggagctgtt caccggggtg
     6601 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     6661 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6721 aagctgcccg tgccctggcc caccctcgtg accaccctga cctatggagt gcagtgcttc
     6781 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6841 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     6901 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     6961 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     7021 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     7081 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     7141 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     7201 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     7261 ggcatggacg agctgtacaa gtctggtggt tctcccaaga agaagaggaa agtctaaccg
     7321 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     7381 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7441 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7501 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7561 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7621 gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7681 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7741 aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacact cagtggaacg
     8701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9781 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat
     9841 ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc
     9901 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     9961 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
    10021 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
    10081 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    10141 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10201 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10261 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatc
//
LOCUS       pRZ5137_oRZ148__2019-11-12_E02.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...6w-b6tm2fo
VERSION     urn.local...6w-b6tm2fo
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..31
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         802..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gcctgtcgac ggtgttgcag caccttgatt tcttgctggg caccttgtac tcgtcggtga
       61 tcacggccca gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtctg
      121 accccccgct gctgcccccg ctgctttcag gtgttgcgct ctcgcttgtg ccaggtgtct
      181 cagagccaga ggagcctccg ctagatcctc cggagtcggt ggagctctgg gccttcttct
      241 gagcattgaa cacctgtcta ggcatccgaa agaaatagca cagcagggcg gcacattcat
      301 ctgccaggat tccctcggta atttcgacgc ggtgattcat gccggggtag tgcagcacgt
      361 ccatcaggga gcctgcggcg ccggtttttg cgttcctcac gccaaacacc acgcggccga
      421 tcctagagtg gatcatggcg ccggcgcaca tcacgcaagg ctcgaatgtg ccgtacaggg
      481 tggcgtcaat cagtctgtag ttctgcatga ccaggccgcc ctgtctcagg gccataattt
      541 cggcatgggc tgttgggtcg tgcaggccga tggctctgtt ccagccctcg ccgatcactc
      601 tattgttcag caccagcacg gctcccacag gcacctccct ctcatcgcgt gccctcttgg
      661 ccagggtcag ggcatgtctc atccagtact cgtgggaaaa ctccacctca gagactttcc
      721 gcttcttctt tggtgactcg aactcgcttc cgtcggctgt ccgtttcatg gtggcggctc
      781 tccctatagt gagtcgtatt agcggccgcg gatctctagc ggatctgacg gttcactaaa
      841 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      901 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      961 ttgacgtcaa tgggggtgag acttggaaat ccccgtgagt
//
LOCUS       pRZ5137_CMVf_2019-11-08_H08.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...4y-b6tm1v7
VERSION     urn.local...4y-b6tm1v7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..46
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         778..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ctggaaggtg gagtctatat agcagagctg gtttagtgaa ccgtcagatc cgctagagat
       61 ccgcggccgc taatacgact cactataggg agagccgcca ccatgaaacg gacagccgac
      121 ggaagcgagt tcgagtcacc aaagaagaag cggaaagtct ctgaggtgga gttttcccac
      181 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga gagggaggtg
      241 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc
      301 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg
      361 gtcatgcaga actacagact gattgacgcc accctgtacg gcacattcga gccttgcgtg
      421 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac
      481 gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg catgaatcac
      541 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgctatttc
      601 tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc caccgactcc
      661 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca
      721 cctgaaagca gcgggggcag cagcgggggg tcagacaaga agtacagcat cggcctggcc
      781 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaagtg cccagcaaga
      841 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
      901 tgctgttcga cagcggcgaa acagccgagg cactcggctg agagaccgcc agaagagata
      961 cacagacgga gaccggatct gctatctgca gagatcttca
//
LOCUS       pRZ5137_oJUL1084__2019-11-12_D04.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...58-b6tm2dy
VERSION     urn.local...58-b6tm2dy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..59
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         774..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 tggtcgtgtg tacgttttcg gagtggcgtg aggacgcaaa accggcgccg caggctccct
       61 gatggacgtg ctgcactacc ccggcatgaa tcaccgcgtc gaaattaccg agggaatcct
      121 ggcagatgaa tgtgccgccc tgctgtgcta tttctttcgg atgcctagac aggtgttcaa
      181 tgctcagaag aaggcccaga gctccaccga ctccggagga tctagcggag gctcctctgg
      241 ctctgagaca cctggcacaa gcgagagcgc aacacctgaa agcagcgggg gcagcagcgg
      301 ggggtcagac aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc
      361 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
      421 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
      481 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
      541 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
      601 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
      661 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
      721 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
      781 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
      841 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
      901 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcagagc
      961 agacggctgg aaaatctgat cgcccagctg ctcgcgagag
//
LOCUS       pRZ5137_oJUL1085__2019-11-12_D05.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...5e-b6tm2e4
VERSION     urn.local...5e-b6tm2e4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..45
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         784..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gggggacttc gcacgagatg gccaggtgga cgacagcttc ttccacagac tggaagagtc
       61 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
      121 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
      181 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
      241 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
      301 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
      361 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa
      421 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
      481 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
      541 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
      601 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
      661 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
      721 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
      781 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
      841 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
      901 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac
      961 cttcgacacg gcagcatccc ccaccagatc cacctggaga
//
LOCUS       pRZ5137_oRZ147__2019-11-12_E01.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...6q-b6tm2fi
VERSION     urn.local...6q-b6tm2fi
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..29
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         785..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 ggaacagagc ggcacccatc ttcggcacat cgtggacgag gtggcctacc acgagaagta
       61 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
      121 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
      181 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
      241 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
      301 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
      361 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
      421 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
      481 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
      541 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
      601 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
      661 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
      721 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
      781 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
      841 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
      901 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttactcatt
      961 cctgaaggac aaccggggaa agatcgagag atcctgactt
//
LOCUS       pRZ5137_oJUL1086__2019-11-12_D06.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...5k-b6tm2ea
VERSION     urn.local...5k-b6tm2ea
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..39
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         755..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gtgcacggcg acagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc
       61 tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta
      121 tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc
      181 agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg
      241 gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg
      301 aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga
      361 agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg
      421 ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg
      481 agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca
      541 gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag
      601 tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga
      661 acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt
      721 ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga
      781 gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg
      841 tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct
      901 ccggcgtgga aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
      961 atcaaggaca aggacttcct ggacatgagg aaaacgagga
//
LOCUS       pRZ5137_oJUL1087__2019-11-12_D07.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...5q-b6tm2eh
VERSION     urn.local...5q-b6tm2eh
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..35
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         704..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 cggctgctgg taacgcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
       61 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
      121 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
      181 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
      241 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
      301 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
      361 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
      421 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
      481 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
      541 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
      601 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
      661 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
      721 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
      781 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
      841 ggcatcctgc agacagtgaa aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
      901 gcccgagaaa catcgtgatc gaatggccag agagaaccag accacccaga aggacagaga
      961 cagtcgcgag agatgagcga tcgagagggc atcaagagct
//
LOCUS       pRZ5137_oJUL1088__2019-11-12_D08.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...5w-b6tm2en
VERSION     urn.local...5w-b6tm2en
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..40
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         677..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 agacgtgctg acctgacctg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
       61 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
      121 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
      181 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
      241 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
      301 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
      361 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
      421 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
      481 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
      541 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
      601 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
      661 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
      721 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
      781 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
      841 tctgaccaag gccgagagag gcggcctgag cgaactggat aagccggctt catcagagac
      901 agctggtgga aacccggcag atcacaagca cgtggcacag atcctgactc ccggatgaca
      961 ctagtacgac gagatgacag ctgatcggag tgagtgatca
//
LOCUS       pRZ5137_oJUL1089__2019-11-12_D09.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...62-b6tm2et
VERSION     urn.local...62-b6tm2et
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..55
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         884..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gtgcaatctc tgcagatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
       61 tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg
      121 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
      181 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
      241 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
      301 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
      361 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
      421 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
      481 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
      541 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
      601 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
      661 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
      721 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
      781 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
      841 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
      901 agaggaacag cgataagctg atcgccagaa gaaggactgg gaccctaaga agtacggcgg
      961 cttcgtgagc cccaccgtgt ctattctgtg ctgtggtggc
//
LOCUS       pRZ5137_oJUL1090__2019-11-12_D10.ab1         999 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...68-b6tm2ez
VERSION     urn.local...68-b6tm2ez
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..262
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         713..999
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gggctgggat ccgtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
       61 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
      121 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
      181 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
      241 caggtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
      301 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
      361 tacggcggct tcgtgagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
      421 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
      481 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
      541 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
      601 aagagaatgc tggcctctgc cagagaactg cagaagggaa acgaactggc cctgccctcc
      661 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctccccccga
      721 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
      781 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
      841 gctgtccgcc tactacaagc accgggataa gcccatcaga gagcaggccg agatatcatc
      901 cacctgttta ccctgaccat ctggagcccc tgccgacttc agtactttga cacagcatcg
      961 accgtagcag tacagagcac aagaggtgct gacgccacc
//
LOCUS       pRZ5137_oJUL1091__2019-11-12_D11.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...6e-b6tm2f6
VERSION     urn.local...6e-b6tm2f6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..15
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         739..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gaggactgat atcagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
       61 atgctggcct ctgccagaga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
      121 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
      181 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
      241 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
      301 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
      361 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
      421 cggaagcagt acagaagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
      481 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgactc tggcggctca
      541 aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctctggagga
      601 tctagcggag gatcctctgg aagcgagaca ccaggcacaa gcgagtccgc cacaccagag
      661 agctccggcg gctcctccgg aggatccaca gacgccgagt acgtgcgcat tcatgagaaa
      721 ctggatattt acaccttcaa gaagcagttc ttcaacaaca agaaatctgt gtcacaccgc
      781 tgctacgtgc tgtttgagtt gaagcgaaag gggcgaaaga aaggcttgct tttggggcta
      841 tgccgtcaac aagccccaaa gtggcaccga gagaggaata cacgctgaga tattcagtat
      901 ccgaaaggtg gaagagtatc ttcgggatat cctggcagtt tacgatcaac tgtattccag
      961 ctgagtctgc gctgatgtgc gagaaatctg atgtatatca
//
LOCUS       pRZ5137_oJUL1094__2019-11-12_D12.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...6k-b6tm2fc
VERSION     urn.local...6k-b6tm2fc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..29
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         761..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 tgacgtggag catctggcgg ctcaaagacc gccgacggca gcgaattcga gcccaagaag
       61 aagaggaaag tctctggagg atctagcgga ggatcctctg gaagcgagac accaggcaca
      121 agcgagtccg ccacaccaga gagctccggc ggctcctccg gaggatccac agacgccgag
      181 tacgtgcgca ttcatgagaa actggatatt tacaccttca agaagcagtt cttcaacaac
      241 aagaaatctg tgtcacaccg ctgctacgtg ctgtttgagt tgaagcgaag gggcgaaaga
      301 agggcttgct tttggggcta tgccgtcaac aagccccaaa gtggcaccga gagaggaata
      361 cacgctgaga tattcagtat ccgaaaggtg gaagagtatc ttcgggataa tcctgggcag
      421 tttacgatca actggtattc cagctggagt ccttgcgctg attgtgccga gaaaattctg
      481 gaatggtata atcaggaact tcggggaaac gggcacacat tgaaaatctg ggcctgcaag
      541 ctgtactacg agaagaatgc ccggaaccag ataggactct ggaatctgag ggacaatggt
      601 gtaggcctga acgtgatggt ttccgagcac tatcagtgtt gtcggaagat tttcatccaa
      661 agctctcata accagctcaa tgaaaaccgc tggttggaga aaacactgaa acgtgcggag
      721 aagtggagat ccgagctgag catcatgatc caggtcaaga ttctgcatac cactaagtct
      781 ccagccgtta gcggcgggag cggcgggagc ggggggagca ctaatctgag cgacatcatt
      841 gagaaggaga ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag
      901 gtggaggaag tgatcggcaa caagccagag tctgacatcc tgtgcacacc gcctacgacg
      961 agtcacagat gagatgtgat gctgctgacc tctgacgccc
//
LOCUS       pRZ5137_oRZ218__2019-11-12_E03.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...72-b6tm2fv
VERSION     urn.local...72-b6tm2fv
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..27
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         943..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 tatgctggag tcttgcgctg attgtgccga gaaaattctg gaatggtata atcaggaact
       61 tcggggaaac gggcacacat tgaaaatctg ggcctgcaag ctgtactacg agaagaatgc
      121 ccggaaccag ataggactct ggaatctgag ggacaatggt gtaggcctga acgtgatggt
      181 ttccgagcac tatcagtgtt gtcggaagat tttcatccaa agctctcata accagctcaa
      241 tgaaaaccgc tggttggaga aaacactgaa acgtgcggag aagtggagat ccgagctgag
      301 catcatgatc caggtcaaga ttctgcatac cactaagtct ccagccgtta gcggcgggag
      361 cggcgggagc ggggggagca ctaatctgag cgacatcatt gagaaggaga ctgggaaaca
      421 gctggtcatt caggagtcca tcctgatgct gcctgaggag gtggaggaag tgatcggcaa
      481 caagccagag tctgacatcc tggtgcacac cgcctacgac gagtccacag atgagaatgt
      541 gatgctgctg acctctgacg cccccgagta taagccttgg gccctggtca tccaggattc
      601 taacggcgag aataagatca agatgctgag cggaggatcc ggaggatctg gaggcagcac
      661 caacctgtct gacatcatcg agaaggagac aggcaagcag ctggtcatcc aggagagcat
      721 cctgatgctg cccgaagaag tcgaagaagt gatcggaaac aagcctgaga gcgatatcct
      781 ggtccatacc gcctacgacg agagtaccga cgaaaatgtg atgctgctga catccgacgc
      841 cccagagtat aagccctggg ctctggtcat ccaggattcc aacggagaga acaaaatcaa
      901 aatgctggga agcggagcta ctaacttcag cctgctgaag cagctggaga cgtggaggag
      961 aaccctggac ctatggtgag caaggcgagg agctgttcac
//
LOCUS       pRZ5137_BGHrev_2019-11-08_D08.ab1        1000 bp    DNA     linear   UNA 12-NOV-2019
DEFINITION  .
ACCESSION   urn.local...4x-b6tm1v7
VERSION     urn.local...4x-b6tm1v7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     trimmed         1..34
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
     trimmed         830..1000
                     /Method="Error probability: 0.001"
                     /label="Trimmed"
ORIGIN      
        1 gtgatggcct actcatggtg atggtgatga tgaccggtta gactttcctc ttcttcttgg
       61 gagaaccacc agacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      121 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      181 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      241 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      301 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      361 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      421 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      481 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      541 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcactcca taggtcaggg
      601 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      661 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      721 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      781 tgctcaccat aggtccaggg ttctcctcca cgtctccagc ctgcttcagc agctgaagtt
      841 agtagctccg cttcccagca ttttgatttt gttctctccg ttggaatcct ggatgaccag
      901 agcccagggc ttatactctg gggcgtcgga tgtcagcagc atcacatttt cgtcggtact
      961 ctcgtcgtag cgtatggacc aggatatcgc tctcagctgt
//
