LOCUS       Exported                8395 bp ds-DNA     circular SYN 04-FEB-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 11, 2020 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  589 ..  657 / #ccffcc / signal sequence
                        2:  658 .. 1449 / #ccffcc
                     Cleavage site after base 657"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /note="color: #ccffcc"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    3295..3322
                     /label=reads ""CGACTGCCC"" in sequencing
                     /note="color: #a6acb3"
     primer_bind     complement(3698..3717)
                     /label=TagSeq-R1
                     /note="color: black; sequence: agcaccttgccgtagaagaa"
     primer_bind     3698..3717
                     /label=TagSeq-F1
                     /note="color: black; sequence: ttcttctacggcaaggtgct"
     misc_feature    3922..4052
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     promoter        4065..4379
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    4073..4091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4109..4127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4144..4162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4180..4198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4216..4234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4251..4269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4287..4305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    4317..4355
                     /label=miniCMV
                     /note="color: #a6acb3"
     primer_bind     complement(4353..4373)
                     /label=TagSeq-R3
                     /note="color: black; sequence: ctgacggttcactaaacgagc"
     primer_bind     4353..4373
                     /label=TagSeq-F3
                     /note="color: black; sequence: GCTCGTTTAGTGAACCGTCAG"
     CDS             4412..5110
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    5163..5751
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(5242..5261)
                     /label=TagSeq-R5
                     /note="color: black; sequence: caaaggcattaaagcagcgt"
     primer_bind     5242..5261
                     /label=TagSeq-F5
                     /note="color: black; sequence: acgctgctttaatgcctttg"
     primer_bind     complement(5417..5436)
                     /label=R screening primer (RAM)
                     /note="color: black; sequence: aaaggagctgacaggtggtg"
     misc_feature    5556..5565
                     /label=sequencing: is ""AAATCATCGT""
                     /note="color: #a6acb3"
     misc_feature    5573
                     /label=sequencing: is ""T""
                     /note="color: #a6acb3"
     misc_feature    5616
                     /label=sequencing: is ""C""
                     /note="color: #a6acb3"
     CDS             complement(5634..5645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 5633"
                     /translation="IEGR"
     misc_feature    5751..5754
                     /label=sequencing: is ""C""
                     /note="color: #a6acb3"
     polyA_signal    5793..5904
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6041..6060)
                     /label=TagSeq-R6
                     /note="color: black; sequence: tattttattgcggcctgtcc"
     primer_bind     6041..6060
                     /label=TagSeq-F6
                     /note="color: black; sequence: GGACAGGCCGCAATAAAATA"
     polyA_signal    6052..6100
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    6114..6205
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="This feature has 2 segments:
                        1: 6114 .. 6203 / #a6acb3
                        2: 6204 .. 6205 / #a6acb3"
     primer_bind     6206..6253
                     /label=F RtW RAM Mlu1
                     /note="color: black; sequence: 
                     GGCCTAACTGGCCGacgcGTACCCTAGAAGTTTG"
     primer_bind     complement(6206..6253)
                     /label=R RtW RAM BamHi
                     /note="color: black; sequence: 
                     CAAACTTCTAGGGTAggatCCGGCCAGTTAGGCC"
     primer_bind     complement(6206..6253)
                     /label=R RtW RAM Mlu1
                     /note="color: black; sequence: 
                     CAAACTTCTAGGGTACgcgtCGGCCAGTTAGGCC"
     primer_bind     6220..6251
                     /label=Mlu1-F
                     /note="color: black; sequence: 
                     GAGTAACGCGTCCTAACTGGCCGGTACCCTAGAAGTT"
     primer_bind     6226..6277
                     /label=F RtW RAM Mlu1
                     /note="color: black; sequence: 
                     GGCCTAACTGGCCGacgcGTACCCTAGAAGTTTG"
     misc_feature    6243..6445
                     /label=pNRAM
                     /note="color: #3366ff; direction: RIGHT"
     misc_feature    6243..6266
                     /label=Varient2
                     /note="color: #ff6600"
     misc_feature    6255..6262
                     /label=NRE
                     /note="color: #a6acb3"
     misc_feature    6267..6290
                     /label=Varient2
                     /note="color: #ff6600"
     misc_feature    6279..6286
                     /label=NRE
                     /note="color: #a6acb3"
     misc_feature    6291..6314
                     /label=Varient2
                     /note="color: #ff6600"
     misc_feature    6303..6310
                     /label=NRE
                     /note="color: #a6acb3"
     misc_feature    6315..6338
                     /label=Varient2
                     /note="color: #ff6600"
     misc_feature    6327..6334
                     /label=NRE
                     /note="color: #a6acb3"
     misc_feature    6346..6445
                     /label=Fos min promoter
                     /note="color: #00ff00"
     primer_bind     complement(6440..6462)
                     /label=BamHI-R
                     /note="color: black; sequence: 
                     AGCTAGGATCCCTAGCGAGCTCGGCCGC"
     misc_feature    6467..6591
                     /label=pNRE_d2EGFPTTA
                     /note="color: #a6acb3"
     misc_feature    6471..6590
                     /label=Degradation domain of mouse ornithine decarboxylas
                     /note="color: #ff99cc"
     misc_feature    6523..6541
                     /label=Untitled
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(6523..6541)
                     /label=TagSeq-R7
                     /note="color: black; sequence: ctctcctgggcacaagaca"
     primer_bind     6523..6541
                     /label=TagSeq-F7
                     /note="color: black; sequence: TGTCTTGTGCCCAGGAGAG"
     CDS             6600..7346
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /note="This feature has 9 segments:
                        1: 6600 .. 7217 / #993366 / TetR
                        2: 7218 .. 7223 / #cc99b2
                        3: 7224 .. 7224 / #993366 / VP16 minimal transcriptio...
                        4: 7225 .. 7259 / #993366 / VP16 minimal transcriptio...
                        5: 7260 .. 7262 / #cc99b2
                        6: 7263 .. 7298 / #993366 / VP16 minimal transcriptio...
                        7: 7299 .. 7301 / #cc99b2
                        8: 7302 .. 7337 / #993366 / VP16 minimal transcriptio...
                        9: 7338 .. 7346 / #cc99b2"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    complement(6703..6722)
                     /label=d2TTA_SeqR4
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    6849..6868
                     /label=d2TTA_SeqF5
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(6849..6868)
                     /label=d2TTA_SeqR5
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    7037..7056
                     /label=Untitled
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     7037..7056
                     /label=TagSeq-F8
                     /note="color: black; sequence: ggaggatcaggagcatcaag"
     misc_feature    complement(7120..7139)
                     /label=d2TTA_SeqR6
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(7288..7307)
                     /label=TagSeq-R8
                     /note="color: black; sequence: gtcagcaggcagcatatcaa"
     primer_bind     complement(7359..7375)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        complement(7401..7419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    7445..7579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   7609..7749
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    7609..7749
                     /label=L-ITR
                     /note="color: #a6acb3"
     polyA_signal    7886..8020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     mobile_element  8049..8214
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca
     3901 gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg
     3961 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
     4021 ggagtggcca actccatcac taggggttcc tgcggccgca tctcgagttt actccctatc
     4081 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     4141 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     4201 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     4261 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     4321 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcca
     4381 ccggtgttta aacgctagcg cggccgccac catggtgagc gagctgatta aggagaacat
     4441 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
     4501 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
     4561 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
     4621 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
     4681 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     4741 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
     4801 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
     4861 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
     4921 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     4981 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
     5041 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
     5101 gcacagatga gtcgacggcg cgccagcgat ccaccggatc tagataactg atccttatcg
     5161 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5221 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5281 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5341 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5401 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5461 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5521 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     5581 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5641 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5701 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgatcgat
     5761 accgtcgact agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5821 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5881 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5941 tggggtgggg caggacagca agggggagga ttgggaagac aatagcagac tagtacgtct
     6001 ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata
     6061 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     6121 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     6181 agtgcaggtg ccagaacatt tctctggcct aactggccga cgcgtcctaa ctggccggta
     6241 ccctagaagt ttgttcgtga ctgtgactag aagtttgttc gtgactgtga ctagaagttt
     6301 gttcgtgact gtgactagaa gtttgttcgt gactgtgaca tgcatcattc ataaaacgct
     6361 tgttataaaa gcagtggctg cggcgcctcg tactccaacc gcatctgcag cgagcaactg
     6421 agaagccaag actgagccgg cggccgagct cgctagggat ccagctcatg agccatggct
     6481 tcccgccgga ggtggaggag caggatgatg gcacgctgcc catgtcttgt gcccaggaga
     6541 gcgggatgga ccgtcaccct gcagcctgtg cttctgctag gatcaatgtg gaattcacca
     6601 tgtctagact ggacaagagc aaagtcataa actctgctct ggaattactc aatgaagtcg
     6661 gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc
     6721 tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctggcaatc gagatgctgg
     6781 acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga
     6841 acaacgccaa gtcattccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc
     6901 tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt
     6961 gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta
     7021 cactgggctg cgtattggag gatcaggagc atcaagtagc aaaagaggaa agagagacac
     7081 ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccatcagg
     7141 gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc
     7201 taaagtgcga aagcggcggg ccggccgacg cccttgacga ttttgactta gacatgctcc
     7261 cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt
     7321 ttgaccttga catgctcccc gggtaactaa gtaagcatcg ataccgtcga cctcgagggg
     7381 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgcagcg gccgaccatg
     7441 gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7501 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7561 atcttatcat gtctggatct ccggaccacg tgcggaccga gcggccgcag gaacccctag
     7621 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     7681 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     7741 gcctgcagga agctgtaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     7801 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     7861 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     7921 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7981 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactga tatcgcctag
     8041 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     8101 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     8161 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
     8221 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
     8281 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
     8341 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatg
//
