LOCUS       Exported                2933 bp ds-DNA     circular SYN 18-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 19, 2020 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        263..279
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    285..312
                     /label=ttgO
                     /bound_moiety="Transcriptional Regulator TtgR"
                     /note="binds homodimeric TtgR"
                     /citation="DOI: 10.1093/nar/gkz772"
     gene            313..464
                     /product="Fluorescence-activating Three Way Junction 
                     dimeric Broccoli (3WJdB) RNA aptamer"
                     /label=Three Way Junction dimeric Broccoli (3WJdB)
     terminator      465..511
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(537..553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1027..1131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1132..1992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2163..2751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gataatacga ctcactatag gaggtattta caaacaacca
      301 tgaatgtaag tacccacata ctctgatgat ccgagacggt cgggtccaga tattcgtatc
      361 tgtcgagtag agtgtgggct cggatcattc atggcaagag acggtcgggt ccagatattc
      421 gtatctgtcg agtagagtgt gggctcttgc catgtgtatg tgggtagcat aaccccttgg
      481 ggcctctaaa cgggtcttga ggggtttttt gatccccggg taccgagctc gaattcactg
      541 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      601 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      661 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
      721 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
      781 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      841 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
      901 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
      961 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1021 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1081 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1141 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1201 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1261 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1321 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1381 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1441 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1501 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1561 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1621 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1681 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1741 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1801 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1861 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1921 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1981 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2821 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2881 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga
//
