LOCUS       GS1.2/35S              11650 bp    DNA     circular UNA 20-FEB-2020
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...u-9j6rhbp
VERSION     urn.local...u-9j6rhbp
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        <1..>635
                     /standard_name="At_UBQ10"
     CDS             664..1374
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed
                     (Shaner et al., 2008)"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     AFQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVM
                     NFEDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDG
                     ALKSEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
                     /standard_name="mApple"
     primer_bind     1077..1096
                     /note="mCherry, forward primer"
                     /standard_name="mCherry-F"
     protein_bind    complement(1377..1401)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /standard_name="attB2"
     misc_feature    1721..1745
                     /note="right border repeat from nopaline C58 T-DNA"
                     /standard_name="RB T-DNA repeat"
     CDS             3076..3705
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
                     /standard_name="pVS1 StaA"
     CDS             4134..5207
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                      pVS1 (Heeb et al., 2000)"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES
                     WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR
                     LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG
                     DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV
                     SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG
                     RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA
                     GQAGPILVMRYRNLIEGEASAGS"
                     /standard_name="pVS1 RepA"
     rep_origin      5273..5467
     primer_bind     complement(5703..5725)
                     /note="pGEX vectors, reverse primer"
                     /standard_name="pGEX 3'"
     misc_feature    5811..5951
                     /note="basis of mobility region from pBR322"
                     /standard_name="bom"
     primer_bind     5866..5885
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /standard_name="pRS-marker"
     primer_bind     complement(5966..5983)
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     rep_origin      complement(6137..6725)
     primer_bind     complement(6217..6236)
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     CDS             complement(6971..7762)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     misc_feature    8283..8307
                     /note="left border repeat from nopaline C58 T-DNA"
                     /standard_name="LB T-DNA repeat"
     terminator      8400..8652
                     /note="nopaline synthase terminator and poly(A) signal"
                     /standard_name="NOS terminator"
     CDS             complement(8675..9247)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP"
                     /standard_name="NrsR"
     promoter        complement(9289..9468)
                     /note="nopaline synthase promoter"
                     /standard_name="NOS promoter"
     regulatory      complement(9578..9830)
                     /regulatory_class="terminator"
                     /note="from nopaline synthase (NOS)"
                     /note="Geneious type: terminator"
                     /standard_name="nopaline synthase (NOS) terminator"
     primer_bind     9578..9602
                     /standard_name="Ligation of pGFPGUSPlus genbank - nopaline
                     synthase (NOS) terminator (reversed) - ADPG PP-350 -
                     mCitrine ligated into pENTR-dTOPO linearised extraction
                     (reversed) into mApple CDS ligated into pN2GW7,0
                     extraction 2 - nopaline synthase (NOS) terminator
                     (reversed) (1 F) - mApple CDS ligated into pN2GW7,0
                     extraction 2"
     primer_bind     9831..9850
                     /standard_name="PGC1-mCit (reversed) (1 F) - Ligation of
                     pGFPGUSPlus genbank - nopaline synthase (NOS) terminator
                     (reversed) - ADPG PP-350 - mCitrine ligated into
                     pENTR-dTOPO linearised extraction (reversed) into mApple
                     CDS ligated into pN2GW7,0 extraction 2 - nopaline synthase
                     (NOS) terminator (reversed)"
     CDS             complement(9843..10559)
                     /standard_name="mCitrine"
     misc_feature    complement(9885..9906)
                     /standard_name="EGFP_C_primer"
     misc_feature    10493..10514
                     /standard_name="EGFP_N_primer"
     misc_feature    complement(<10560..10567)
                     /note="Geneious type: attR1"
                     /standard_name="attR1"
     misc_feature    10560..10567
                     /standard_name="Remaining bit after clonase rxn"
     primer_bind     10560..10583
                     /standard_name="CaMV35S (reversed) (1 F) - GS1.2
                     extraction"
     misc_feature    complement(10595..11621)
                     /note="Geneious type: promoter uukaryotic"
                     /standard_name="p35S"
ORIGIN      
        1 tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt
       61 tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac
      121 aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac
      181 cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac
      241 aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa
      301 ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa
      361 aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct
      421 gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc
      481 gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat
      541 tactcttcga tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga
      601 atttgtcgat taatctgagt ttttctgatt aacaggcggc cgcactagtg atatcacaag
      661 tttatggtga gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc
      721 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      781 ggccgcccct acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg
      841 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag
      901 cacccagccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag
      961 cgcgtgatga acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag
     1021 gacggcgtgt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1081 gtaatgcaga agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac
     1141 ggcgccctga agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc
     1201 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
     1261 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac
     1321 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatcgttc
     1381 agctttcttg tacaaagtgg tgatatcccg cggccatgct agagtccgca aaaatcacca
     1441 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     1501 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     1561 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     1621 caaaatccag tgacctgcag gcatgcgacg tcgggcccaa gcttagcttg agcttggatc
     1681 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     1741 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     1801 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     1861 gtactttaaa gtactttaaa gtactttaaa gtactttgat ccaacccctc cgctgctata
     1921 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
     1981 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     2041 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     2101 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     2161 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     2221 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     2281 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     2341 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     2401 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     2461 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     2521 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     2581 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     2641 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     2701 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     2761 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     2821 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     2881 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     2941 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     3001 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     3061 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     3121 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     3181 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     3241 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     3301 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     3361 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     3421 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     3481 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     3541 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     3601 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     3661 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     3721 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     3781 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     3841 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     3901 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     3961 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     4021 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     4081 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     4141 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     4201 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     4261 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     4321 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     4381 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     4441 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     4501 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     4561 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     4621 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     4681 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     4741 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     4801 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     4861 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     4921 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     4981 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     5041 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     5101 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     5161 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     5221 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     5281 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     5341 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     5401 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     5461 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     5521 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     5581 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     5641 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     5701 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5761 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     5821 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     5881 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5941 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6001 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6061 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6121 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6181 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6241 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6301 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6361 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6421 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6481 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6541 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6601 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6661 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6721 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6781 gttaagggat tttggtcatg catgatatat ctcccaattt gtgtagggct tattatgcac
     6841 gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt
     6901 agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg aacgaatttc
     6961 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     7021 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     7081 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     7141 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     7201 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     7261 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     7321 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     7381 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     7441 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     7501 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     7561 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     7621 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     7681 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     7741 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg
     7801 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     7861 tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg
     7921 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     7981 tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgccc
     8041 gaattgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     8101 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     8161 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     8221 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     8281 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     8341 cggacgtttt taatgtactg aattaacgcc gaattgaatt atcagcttgc atgccggtcg
     8401 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     8461 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     8521 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
     8581 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
     8641 gtttgaacga tctgcttgac tctaggggtc atcattaggg gcagggcatg ctcatgtaga
     8701 gcgcctgctc gccgtccgag gcggtgccgt cgtacagggc ggtgtccagg ccgcagaggg
     8761 tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt gacgttggtg acctccagcc
     8821 agaggtgccc ggcaccccgc tcgcgggcga actccgtcgc gagccccatc aacgcgcgcc
     8881 cgaccccgtg cccccggtgc tccggggcga cctcgatgtc ctcgacggtc agccggcggt
     8941 tccagccgga gtacgagacg accacgaagc ccgccaggtc gccgtcgtcc ccgtacgcga
     9001 cgaacgtccg agagtccggg tcgccgtcct ccccgtcgtc cgattcgtcg tccgattcgt
     9061 cgtcggggaa caccttggtc aggggcgggt ccaccggcac ctcccgcagg gtgaagccgt
     9121 ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa ggacccatcc agtgcctcga
     9181 tggcctcggc gtcccccggg acactggtgc ggtaccggta agccgtgtcg tcaagagtgg
     9241 tacccatggt agatcccctc gatcgagttg agagtgaata tgagactcta attggatacc
     9301 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     9361 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     9421 taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa
     9481 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
     9541 cgctcatgat cgataattcg agctctccca tatggtcgat ctagtaacat agatgacacc
     9601 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     9661 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     9721 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     9781 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc cgcccaccct
     9841 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     9901 accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactggta gctcaggtag
     9961 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
    10021 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
    10081 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
    10141 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
    10201 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
    10261 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
    10321 ttcatgtggt cggggtagcg ggcgaagcac atcaggccgt agccgaaggt ggtcacgagg
    10381 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
    10441 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
    10501 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccata
    10561 aacttgtgat atcactagtg cggccgcctg caggtcgact agaatagtaa attgtaatgt
    10621 tgtttgttgt ttgttttgtt gtggtattgt tgtaaaaata ccggagtcct ctccaaatga
    10681 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
    10741 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
    10801 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
    10861 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
    10921 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
    10981 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
    11041 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgaa gattttcttc
    11101 ttgtcattga gtcgtaaaag actctgtatg aactgttcgc cagtcttcac ggcgagttct
    11161 gttagatcct cgatctgaat ttttgactcc atggcctttg attcagtagg aactactttc
    11221 ttagagactc caatctctat tacttgcctt ggtttatgaa gcaagccttg aatcgtccat
    11281 actggaatag tacttctgat cttgagaaat atatctttct ctgtgttctt gatgcagtta
    11341 gtcctgaatc ttttgactgc atctttaacc ttcttgggaa ggtatttgat ctcctggaga
    11401 ttattactcg ggtagatcgt cttgatgaga cctgccgcgt aggcctctct aaccatctgt
    11461 gggtcagcat tctttctgaa attgaagagg ctaatcttct cattatcggt ggtgaacatg
    11521 gtatcgtcac cttctccgtc gaactttctt cctagatcgt agagatagag aaagtcgtcc
    11581 atggtgatct ccggggcaaa ggagatcagc ttggctctag ttataaaaaa gcattgctca
    11641 tcaatttgtt
//
