LOCUS       Exported               11289 bp ds-DNA     circular SYN 18-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   LQP
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 20, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..1999
                     /label=ZmUbi1
     CDS             2009..2029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2030..6130
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6137..6157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6164..6259
                     /codon_start=1
                     /label=32aa-linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             6266..8296
                     /codon_start=1
                     /label=M-MLV
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
     CDS             8309..8329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8389..8565
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9119..9135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9143..9159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9167..9197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9212..9233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9521..10109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10280..11140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11141..11245)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttatgcc taagaaaaag agaaaagtgg acaagaagta
     2041 ctcgatcggc ctcgatattg ggactaactc tgttggctgg gccgtgatca ccgacgagta
     2101 caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa
     2161 gaatctcatt ggcgctctcc tgttcgacag cggcgagacg gctgaggcta cgcggctcaa
     2221 gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc atctgctacc tgcaggagat
     2281 tttctccaac gagatggcga aggttgacga ttctttcttc cacaggctgg aggagtcatt
     2341 cctcgtggag gaggataaga agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga
     2401 ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc
     2461 cacagataag gcggacctcc gcctgatcta cctcgctctg gcccacatga ttaagttcag
     2521 gggccatttc ctgatcgagg gggatctcaa cccggacaat agcgatgttg acaagctgtt
     2581 catccagctc gtgcagacgt acaaccagct cttcgaggag aaccccatta atgcgtcagg
     2641 cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag tctcggcgcc tcgagaacct
     2701 gatcgcccag ctgccgggcg agaagaagaa cggcctgttc gggaatctca ttgcgctcag
     2761 cctggggctc acgcccaact tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca
     2821 gctctccaag gacacatacg acgatgacct ggataacctc ctggcccaga tcggcgatca
     2881 gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc tgtctgatat
     2941 tctcagggtg aacaccgaga ttacgaaggc tccgctctca gcctccatga tcaagcgcta
     3001 cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtcaggcagc agctccccga
     3061 gaagtacaag gagatcttct tcgatcagtc gaagaacggc tacgctgggt acattgacgg
     3121 cggggcctct caggaggagt tctacaagtt catcaagccg attctggaga agatggacgg
     3181 cacggaggag ctgctggtga agctcaatcg cgaggacctc ctgaggaagc agcggacatt
     3241 cgataacggc agcatcccac accagattca tctcggggag ctgcacgcta tcctgaggag
     3301 gcaggaggac ttctaccctt tcctcaagga taaccgcgag aagatcgaga agattctgac
     3361 tttcaggatc ccgtactacg tcggcccact cgctaggggc aactcccgct tcgcttggat
     3421 gacccgcaag tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg
     3481 cgctagcgct cagtcgttca tcgagaggat gacgaatttc gacaagaacc tgccaaatga
     3541 gaaggtgctc cctaagcact cgctcctgta cgagtacttc acagtctaca acgagctgac
     3601 taaggtgaag tatgtgaccg agggcatgag gaagccggct ttcctgtctg gggagcagaa
     3661 gaaggccatc gtggacctcc tgttcaagac caaccggaag gtcacggtta agcagctcaa
     3721 ggaggactac ttcaagaaga ttgagtgctt cgattcggtc gagatctctg gcgttgagga
     3781 ccgcttcaac gcctccctgg ggacctacca cgatctcctg aagatcatta aggataagga
     3841 cttcctggac aacgaggaga atgaggatat cctcgaggac attgtgctga cactcactct
     3901 gttcgaggac cgggagatga tcgaggagcg cctgaagact tacgcccatc tcttcgatga
     3961 caaggtcatg aagcagctca agaggaggag gtacaccggc tgggggaggc tgagcaggaa
     4021 gctcatcaac ggcattcggg acaagcagtc cgggaagacg atcctcgact tcctgaagag
     4081 cgatggcttc gcgaaccgca atttcatgca gctgattcac gatgacagcc tcacattcaa
     4141 ggaggatatc cagaaggctc aggtgagcgg ccagggggac tcgctgcacg agcatatcgc
     4201 gaacctcgct ggctcgccag ctatcaagaa ggggattctg cagaccgtga aggttgtgga
     4261 cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg agatggcccg
     4321 ggagaatcag accacgcaga agggccagaa gaactcacgc gagaggatga agaggatcga
     4381 ggagggcatt aaggagctgg ggtcccagat cctcaaggag cacccggtgg agaacacgca
     4441 gctgcagaat gagaagctct acctgtacta cctccagaat ggccgcgata tgtatgtgga
     4501 ccaggagctg gatattaaca ggctcagcga ttacgacgtc gatgccatcg ttccacagtc
     4561 attcctgaag gatgactcca ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg
     4621 caagtctgat aatgttcctt cagaggaggt cgttaagaag atgaagaact actggcgcca
     4681 gctcctgaat gccaagctga tcacgcagcg gaagttcgat aacctcacaa aggctgagag
     4741 gggcgggctc tctgagctgg acaaggcggg cttcatcaag aggcagctgg tcgagacacg
     4801 gcagatcact aagcacgttg cgcagattct cgactcacgg atgaacacta agtacgatga
     4861 gaatgacaag ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc tcgtctccga
     4921 cttcaggaag gatttccagt tctacaaggt tcgggagatc aacaattacc accatgccca
     4981 tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc aagaagtacc caaagctcga
     5041 gagcgagttc gtgtacgggg actacaaggt ttacgatgtg aggaagatga tcgccaagtc
     5101 ggagcaggag attggcaagg ctaccgccaa gtacttcttc tactctaaca ttatgaattt
     5161 cttcaagaca gagatcactc tggccaatgg cgagatccgg aagcgccccc tcatcgagac
     5221 gaacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa
     5281 ggttctctcc atgccacaag tgaatatcgt caagaagaca gaggtccaga ctggcgggtt
     5341 ctctaaggag tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga
     5401 ctgggatccg aagaagtacg gcgggttcga cagccccact gtggcctact cggtcctggt
     5461 tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctgctggg
     5521 gatcacgatt atggagcgct ccagcttcga gaagaacccg atcgatttcc tggaggcgaa
     5581 gggctacaag gaggtgaaga aggacctgat cattaagctc cccaagtact cactcttcga
     5641 gctggagaac ggcaggaagc ggatgctggc ttccgctggc gagctgcaga aggggaacga
     5701 gctggctctg ccgtccaagt atgtgaactt cctctacctg gcctcccact acgagaagct
     5761 caagggcagc cccgaggaca acgagcagaa gcagctgttc gtcgagcagc acaagcatta
     5821 cctcgacgag atcattgagc agatttccga gttctccaag cgcgtgatcc tggccgacgc
     5881 gaatctggat aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa tcagggagca
     5941 ggctgagaat atcattcatc tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa
     6001 gtacttcgac acaactatcg atcgcaagag gtacacaagc actaaggagg tcctggacgc
     6061 gaccctcatc caccagtcga ttaccggcct ctacgagacg cgcatcgacc tgtctcagct
     6121 cgggggcgac gaattcccaa agaagaagcg gaaggtggag ctcagcggag gatcttccgg
     6181 aggatctagc ggctccgaga caccaggaac atccgaaagc gctacaccag aatctagcgg
     6241 aggctcttcc ggaggatcta ggcctaccct caacatcgag gatgagtatc gcctccacga
     6301 aacctccaaa gaaccggacg tgtccctcgg cagcacatgg ctcagcgact tcccacaagc
     6361 gtgggccgaa accggcggca tgggcctcgc cgtccgccaa gccccactca ttatcccgct
     6421 gaaggcgacc tccacaccgg tgtccatcaa gcagtacccg atgagccaag aggcgaggct
     6481 cgggattaag ccgcacattc agcgcctcct cgatcaaggc attctcgtgc cgtgccaatc
     6541 cccgtggaat acaccactcc tcccggtcaa aaagccgggc accaacgact atcgcccggt
     6601 ccaagatctc cgcgaggtca acaagcgcgt ggaagacatc cacccgaccg tcccgaaccc
     6661 gtataatctg ctctccgggc tcccaccatc ccaccagtgg tatacagtgc tggacctcaa
     6721 agacgccttc ttctgtctcc gcctccaccc aacaagccag ccgctcttcg ccttcgagtg
     6781 gcgcgacccg gagatgggca tctccggcca actgacatgg acacgcctcc cgcaaggctt
     6841 caagaacagc ccgacactct tcaacgaggc gctccatagg gacctcgcgg attttcgcat
     6901 ccagcatccg gacctcatcc tcctccagta tgtggatgat ctcctcctcg ccgcgacctc
     6961 cgagctggat tgtcaacaag gcacacgcgc gctcctccaa acactcggga acctcggcta
     7021 tcgcgcgtcc gcgaaaaagg cccaaatctg ccagaagcaa gtgaagtacc tcgggtatct
     7081 gctcaaggaa ggccaacgct ggctcaccga agcgcgcaaa gaaacagtga tggggcaacc
     7141 gacaccgaaa acaccacgcc agctgcgcga gtttctcggc aaagccggct tctgtcgcct
     7201 cttcatcccg ggctttgccg agatggccgc gccactctac ccactcacca agccgggcac
     7261 actgtttaac tgggggccgg atcagcagaa agcctaccaa gagatcaaac aagcgctcct
     7321 caccgcccca gcgctcgggc tcccagatct cacaaagccg ttcgagctgt tcgtcgatga
     7381 gaagcaaggc tacgcgaagg gcgtgctcac acagaagctc ggcccgtgga ggaggccagt
     7441 ggcctatctc tccaaaaaac tcgatccagt ggccgccggc tggccaccgt gtctgcgcat
     7501 ggtcgccgcg attgccgtgc tcacaaagga tgccggcaaa ctcacaatgg gccagccgct
     7561 ggtgatcctc gcgccacatg ccgtggaagc cctcgtcaaa cagccgccgg ataggtggct
     7621 ctccaatgcg cgcatgaccc attaccaagc gctcctcctc gacaccgatc gcgtccagtt
     7681 cggcccagtg gtcgccctca atccggcgac actgctgcca ctcccagagg agggcctcca
     7741 acacaactgt ctggatattc tcgcggaagc gcatggcaca aggccagacc tcacagatca
     7801 accgctcccg gatgcggatc acacatggta taccgacggc tcctctctgc tccaagaggg
     7861 ccaaaggaaa gccggcgccg cggtgaccac agaaacagaa gtgatctggg ccaaggccct
     7921 cccagccggc acatccgcgc aaagggcgga actcatcgcg ctcacacaag ccctcaagat
     7981 ggccgagggc aagaagctca acgtctacac agactcccgc tatgccttcg ccaccgccca
     8041 cattcacggc gaaatctata ggaggcgcgg ctggctcaca agcgagggga aggagatcaa
     8101 gaacaaggat gagatcctcg cgctgctcaa ggccctcttt ctcccgaagc gcctctccat
     8161 catccactgt ccgggccacc aaaaggggca ctccgcggaa gcgaggggca ataggatggc
     8221 cgatcaagcc gcgcgcaaag ccgcgattac cgaaacccca gacacatcca ccctcctcat
     8281 cgaaaactcc tccccaagcg gcggcagccc gaagaagaaa aggaaggtgt gagcggccgc
     8341 ggtacgctga aatcaccagt ctctctctac aaatctatct ctctctattt tctccataaa
     8401 taatgtgtga gtagtttccc gataagggaa attagggttc ttatagggtt tcgctcatgt
     8461 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     8521 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatctccta aagtccctat
     8581 agatctttgt cgtgaatata aaccagacac gagacgacta aacctggagc ccagacgccg
     8641 ttcgaagcta gaagtaccgc ttaggcagga ggccgttagg gaaaagatgc taaggcaggg
     8701 ttggttacgt tgactccccc gtaggtttgg tttaaatatg atgaagtgga cggaaggaag
     8761 gaggaagaca aggaaggata aggttgcagg ccctgtgcaa ggtaagaaga tggaaatttg
     8821 atagaggtac gctactatac ttatactata cgctaaggga atgcttgtat ttatacccta
     8881 taccccctaa taacccctta tcaatttaag aaataatccg cataagcccc cgcttaaaaa
     8941 ttggtatcag agccatgaat aggtctatga ccaaaactca agaggataaa acctcaccaa
     9001 aatacgaaag agttcttaac tctaaagata aaagatcttt caagatcaaa actagttccc
     9061 tcacaccggt gacggggatc gcatgcgata tctcgagatc tagcttggcg taatcatggt
     9121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gccagtgcca
    11281 agctaattc
//
