LOCUS Exported 2866 bp ds-DNA circular SYN 20-FEB-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2866) AUTHORS LQP TITLE Direct Submission JOURNAL Exported Thursday, Feb 20, 2020 from SnapGene Viewer 5.0.7 https://www.snapgene.com FEATURES Location/Qualifiers source 1..2866 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 80..668 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 916..1316 /label=TaU3 promoter (400bp upstream) misc_RNA 1340..1415 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind 1426..1442 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" polyA_signal 1455..1629 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" misc_feature 1746..2799 /label=AmpR promoter 1787..1878 /gene="bla" /label=AmpR promoter ORIGIN 1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt 181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc 421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 841 aagcgggaga gcgcccacct gccagggcag cgaagagcaa gccagataac agtatgcgta 901 tttgcgcgct tcgaaggcgg cagggagagt tttaacattg actagcgtgc tgataatttg 961 tgagaaataa taattgacaa gtagatactg acatttgaga agagcttctg aactgttatt 1021 agtaacaaaa atggaaagct gatgcacgga aaaaggaaag aaaaagccat actttttttt 1081 aggtaggaaa agaaaaagcc atacgagact gatgtctctc agatgggccg ggatctgtct 1141 atctagcagg cagcagccct accaacctca cgggccagca attacgagtc cttctaaaac 1201 gtcccgccga gggcgcgtgg ccgtgctgtg cagcagcacg tctaacatta gtcccacctc 1261 gccagtttac agggagcaga accagcttat aagcggaggc gcggcaccaa gaagcgagag 1321 acggagtcga ttcgtctcgg ttttagagct agaaatagca agttaaaata aggctagtcc 1381 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttttcagga aacagctatg 1441 acccatggct cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg 1501 gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta 1561 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa 1621 atccagatcc cccgaattag agctcgcgcg tagtcctcgg taatatcgca gaacaaaagt 1681 acctgatatc gagtgtactt caagtcacac cggcgagtga ctagcaggtg atgaaaagcc 1741 ttagggtcag gtggcacttt tcggggaaat gtgcgatgaa cccctatttg tttatttttc 1801 taaatacatt caaatatgta tccgctaatg agacaataac cctgataaat gcttcaatta 1861 tattgaaaaa ggaagagtat gagtatccaa catttccgtg tcgcccttat tccctttttt 1921 gcggccttct gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 1981 gaagatcagt tgggtgcccg agtgggttac attgaactgg atctcaacag cggcaaaatc 2041 ttagagagtt ttcgccccga agaacgcttc ccaatgatga gcactttcaa agttctgcta 2101 tgtggcgcgg tgttatcccg tattgatgct gggcaagagc aactcggtcg ccgcatacac 2161 tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct tacggatggc 2221 atgacagtaa gagaattatg tagtgctgcc ataaccatga gtgataacac tgcggccaac 2281 ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca caacatgggg 2341 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 2401 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaactc tccgcaaact attaactggc 2461 gaactactta ctcttgcttc acgccagcaa ctcattgact ggatggaggc ggacaaagtt 2521 gcaggaccac ttctgcgctc ggcacttccg gctggctggt ttattgctga taaatctgga 2581 gccggtgagc gtggctctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 2641 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 2701 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacga agggaaataa 2761 atagtagccc gcctgatgtg cgggcttttt tttggtggtg gttaccagtg tcgcttagct 2821 accgtaggtc ctcttaggcg gtccgattag cgacatagtc atcaat //