LOCUS       Exported                2866 bp ds-DNA     circular SYN 20-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   LQP
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 20, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        916..1316
                     /label=TaU3 promoter (400bp upstream)
     misc_RNA        1340..1415
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1426..1442
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1455..1629
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1746..2799
                     /label=AmpR
     promoter        1787..1878
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcgggaga gcgcccacct gccagggcag cgaagagcaa gccagataac agtatgcgta
      901 tttgcgcgct tcgaaggcgg cagggagagt tttaacattg actagcgtgc tgataatttg
      961 tgagaaataa taattgacaa gtagatactg acatttgaga agagcttctg aactgttatt
     1021 agtaacaaaa atggaaagct gatgcacgga aaaaggaaag aaaaagccat actttttttt
     1081 aggtaggaaa agaaaaagcc atacgagact gatgtctctc agatgggccg ggatctgtct
     1141 atctagcagg cagcagccct accaacctca cgggccagca attacgagtc cttctaaaac
     1201 gtcccgccga gggcgcgtgg ccgtgctgtg cagcagcacg tctaacatta gtcccacctc
     1261 gccagtttac agggagcaga accagcttat aagcggaggc gcggcaccaa gaagcgagag
     1321 acggagtcga ttcgtctcgg ttttagagct agaaatagca agttaaaata aggctagtcc
     1381 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttttcagga aacagctatg
     1441 acccatggct cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg
     1501 gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     1561 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
     1621 atccagatcc cccgaattag agctcgcgcg tagtcctcgg taatatcgca gaacaaaagt
     1681 acctgatatc gagtgtactt caagtcacac cggcgagtga ctagcaggtg atgaaaagcc
     1741 ttagggtcag gtggcacttt tcggggaaat gtgcgatgaa cccctatttg tttatttttc
     1801 taaatacatt caaatatgta tccgctaatg agacaataac cctgataaat gcttcaatta
     1861 tattgaaaaa ggaagagtat gagtatccaa catttccgtg tcgcccttat tccctttttt
     1921 gcggccttct gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1981 gaagatcagt tgggtgcccg agtgggttac attgaactgg atctcaacag cggcaaaatc
     2041 ttagagagtt ttcgccccga agaacgcttc ccaatgatga gcactttcaa agttctgcta
     2101 tgtggcgcgg tgttatcccg tattgatgct gggcaagagc aactcggtcg ccgcatacac
     2161 tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct tacggatggc
     2221 atgacagtaa gagaattatg tagtgctgcc ataaccatga gtgataacac tgcggccaac
     2281 ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2341 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2401 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaactc tccgcaaact attaactggc
     2461 gaactactta ctcttgcttc acgccagcaa ctcattgact ggatggaggc ggacaaagtt
     2521 gcaggaccac ttctgcgctc ggcacttccg gctggctggt ttattgctga taaatctgga
     2581 gccggtgagc gtggctctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2641 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2701 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacga agggaaataa
     2761 atagtagccc gcctgatgtg cgggcttttt tttggtggtg gttaccagtg tcgcttagct
     2821 accgtaggtc ctcttaggcg gtccgattag cgacatagtc atcaat
//
