LOCUS       Exported                9583 bp ds-DNA     circular SYN 24-FEB-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 143 .. 148 / #ffffff / -35
                        2: 149 .. 166 / #ffffff
                        3: 167 .. 173 / #ffffff / -10"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    207..225
                     /label=M13 REV
                     /note="color: #ff8000; direction: RIGHT"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    295..1098
                     /label=AAVS1 arm 1
                     /note="color: #ff99cc"
     enhancer        1155..1458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        1459..1662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1688..1736
                     /label=LacZ_misc_feature
                     /note="note=""LacZ"" - 146..493"
                     /note="color: #dc143c; direction: RIGHT"
     CDS             1700..3427
                     /codon_start=1
                     /label=AtAFB2
                     /note="color: #0000ff"
                     /translation="MNYFPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFI
                     GNCYAINPERLLRRFPCLKSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEE
                     LRLKRMVVTDESLELLSRSFVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEI
                     DDHRGQWLSCFPDTCTTLVTLNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDAL
                     ARLMACAPQIVDLGVGSYENDPDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHP
                     ICHNLTSLNLSYAAEIHGSHLIKLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELR
                     VFPSDLLGGGNTAVTEEGLVAISAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRL
                     CILEPNKPDHVTSQPLDEGFGAIVKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSI
                     AFAGDTDKGMLYVLNGCKKMKKLEIRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSG
                     CKRLAEKAPWLNVEIINENDNNRMEENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL"
     misc_feature    2119..2649
                     /label=ori_misc_feature [*]
                     /note="note=""ori"" - 876..1490"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    2650
                     /label=ori_misc_feature [*] [5']
                     /note="note=""ori"" - 876..1490"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    2652..2733
                     /label=ori_misc_feature [*] [3']
                     /note="note=""ori"" - 876..1490"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    complement(2893..3448)
                     /label=Amp+_misc_feature
                     /note="note=""Amp+"" - complement(1650..2510)"
                     /note="color: #dc143c; direction: LEFT"
     polyA_signal    3594..3715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        3724..4224
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4246..4845
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    4852..5076
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    5079..5915
                     /label=AAVS1 arm 2
                     /note="color: #ff99cc"
     promoter        complement(5956..5974)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5981..5997)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    5981..5997
                     /label=M13 FOR
                     /note="color: #ff8000; direction: RIGHT"
     CDS             6135..6437
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             6786..7580
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7830..8690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7830 .. 8621 / #ccffcc
                        2: 8622 .. 8690 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8814..9402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1141 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1201 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1261 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1321 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1381 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1441 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1501 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1561 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1621 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     1681 atccgctagc gctgccacca tgaactattt tcctgacgag gtgattgagc acgtctttga
     1741 cttcgtgacc agccacaagg atcggaacgc tattagcctg gtgtgcaagt catggtacaa
     1801 gatcgagaga tactctaggc agaaagtctt cattggcaac tgttatgcta tcaaccccga
     1861 gcggttgctg cggcggttcc cctgcctgaa gtctctcacc ttgaagggga agccccactt
     1921 cgcagacttt aacctggtgc cacatgagtg gggcgggttt gtgctgccat ggattgaggc
     1981 cttggcccgg tccagggtgg ggctggagga gcttaggctc aagagaatgg tggtcaccga
     2041 tgagtccctg gagctgctca gcaggtcctt tgtcaacttc aagagcctcg tgctcgtcag
     2101 ctgcgagggg ttcaccactg atgggctggc atctatcgcc gctaactgta gacacctgag
     2161 ggatctcgat ctgcaggaaa atgaaatcga cgatcacaga gggcagtggt tgtcatgttt
     2221 ccccgacact tgcactaccc tggtcaccct taactttgcc tgcctggagg gcgaaaccaa
     2281 cctggtggcc ctcgaacggc tggtggcaag gagcccaaac ctcaaatctt tgaagctcaa
     2341 ccgggccgtc cccctggatg ctctggctag gttgatggcc tgcgcccctc agattgtgga
     2401 cctgggcgtc ggctcctacg agaatgaccc tgactccgag agctacctca agctgatggc
     2461 cgtgatcaag aaatgtacaa gcttgagaag cctgtcaggc tttctggaag ccgccccaca
     2521 ttgtctgtca gccttccacc ccatttgcca caatctcaca agcctgaacc tctcctacgc
     2581 cgctgagatc catggcagcc acctcatcaa actgattcag cactgcaaga agctgcagag
     2641 gttgtggatt ctggatagca tcggagacaa aggactggag gtggtggcct ccacctgcaa
     2701 agagctgcag gagctgaggg tgtttccctc cgacctgctc ggggggggga acacagccgt
     2761 gacagaggaa ggcctggtgg ccatctccgc cggatgcccc aagttgcact ccatcctgta
     2821 cttctgtcag cagatgacta acgccgctct tgtgacagtg gccaagaact gccccaactt
     2881 tattaggttc aggttgtgca tccttgagcc taacaagcct gaccacgtca caagtcagcc
     2941 cctggacgag ggcttcgggg caatcgtcaa ggcctgcaag tcccttaggc ggcttagcct
     3001 ctccggactg ctgaccgacc aggtcttcct ttacattggg atgtacgcca accaactgga
     3061 gatgctgagc attgcttttg ctggcgatac agataagggg atgctctacg tgctcaacgg
     3121 ctgcaagaaa atgaagaagt tggagatccg ggatagccct ttcggagata cagcccttct
     3181 cgctgatgtg agcaagtacg agactatgcg cagtctgtgg atgagtagtt gcgaggtgac
     3241 actgtccggc tgcaagcgcc tcgccgagaa ggccccctgg ctcaacgtgg agattatcaa
     3301 cgagaacgac aacaaccgca tggaggaaaa cggccatgaa ggacggcaga aggtcgataa
     3361 gctgtacctg tacagaaccg tcgtcgggac acggatggat gccccaccat ttgtgtggat
     3421 tttgtgagga tccgacgggc ccgtcgacgg taccgcgggc ccgggatcca ccggggccgc
     3481 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3541 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3601 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3661 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaagttg
     3721 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     3781 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     3841 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     3901 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     3961 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     4021 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     4081 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     4141 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     4201 cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag
     4261 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     4321 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4381 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4441 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4501 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4561 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4621 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     4681 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     4741 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     4801 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaggtac cctgtgcctt
     4861 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4921 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4981 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5041 atagcaggca tgctggggat gcggtgggct ctatggttac tagggacagg attggtgaca
     5101 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     5161 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     5221 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     5281 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     5341 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     5401 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     5461 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     5521 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     5581 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     5641 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     5701 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     5761 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     5821 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     5881 tgccccttcc ctacaggggt tcctggctct gctctaaggg caagggcgaa ttcgcggccg
     5941 ctaaattcaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     6001 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6061 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6121 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
     6181 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg
     6241 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
     6301 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
     6361 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
     6421 tgttctgggg aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc
     6481 ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     6541 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     6601 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     6661 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
     6721 cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt
     6781 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6841 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6901 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6961 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     7021 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     7081 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     7141 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     7201 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     7261 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     7321 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     7381 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     7441 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7501 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7561 ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc
     7621 ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc
     7681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagatta
     7741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgaccaaaat cccttaacgt
     8761 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8941 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
