LOCUS       Exported                9179 bp ds-DNA     circular SYN 24-FEB-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Molecular Function Laboratory
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 143 .. 148 / #ffffff / -35
                        2: 149 .. 166 / #ffffff
                        3: 167 .. 173 / #ffffff / -10"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     240..270
                     /label=oTK425
                     /note="color: black; sequence: 
                     gcgcgcggtaccTTCAGCGAGAGGATCAAACAGCTGC"
     misc_feature    246..1296
                     /label=DHC1 homology
                     /note="color: #ff99cc"
     misc_feature    806..933
                     /label=85544-85671
                     /note="color: #ffff99; direction: RIGHT"
     primer_bind     825..844
                     /label=oTK434
                     /note="color: black; sequence: CACGTGCAACAACAACAAGC"
     CDS             1170..1295
                     /codon_start=1
                     /label=85908-86036 (last exon)
                     /note="color: #ffff99"
                     /translation="VTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE"
     primer_bind     complement(1235..1280)
                     /label=oTK440
                     /note="color: black; sequence: 
                     TGCGACACCtCttTCGTAGAAcgaGCGAGGgTCCTCCTTTGTAGCA"
     misc_feature    1251..1270
                     /label=sgRNA target
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    1267
                     /label=cleavage
                     /note="color: #ff0000"
     misc_feature    1271..1273
                     /label=PAM(mutation)
                     /note="color: #800080"
     CDS             1296
                     /codon_start=1
                     /label=Linker
                     /note="note=""direct strand 
                     translation""translation=""GAGA"""
                     /note="color: #ffcc99"
                     /translation=""
     CDS             1297..1313
                     /codon_start=1
                     /label=linker
                     /note="color: #ff99cc"
                     /translation="SGAGA"
     CDS             1314..1517
                     /codon_start=1
                     /label=miniIAA7
                     /note="color: #3366ff"
                     /translation="GFSETVDLMLNLQSNKEGSVDLKNVSAVPKEKTTLKDPSKPPAKA
                     QVVGWPPVRNYRKNMMTQQKTSS"
     CDS             complement(1522..2219)
                     /codon_start=1
                     /label=ORF frame 1
                     /note="color: #993366"
                     /translation="MCNPGGGHELQQDHVIALLVGVFAQLGLMAQVVVVGQQHGAVADG
                     GVLLVVVGELHAAVLNVVADLEVSLDAVLLLVGRDIDVVAVEVVLQLVPQDVAVLLEVD
                     ALQLDAVHQGVALELHLGAGLVGTVVLERDGALLDVAFGHGGLEEVVLLHVVGVAAEAG
                     HAVAEGGHEGGPGHGQLAGGADELQGQLAVGGIALALAADAELVAVYVAVQLDQDGHHP
                     GEQLLALAHH"
     CDS             1524..2241
                     /codon_start=1
                     /label=ORF frame 2
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             1524..2240
                     /codon_start=1
                     /label=mClover(A206K)
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     gene            1527..2240
                     /gene="EGFP (variant)"
                     /label=EGFP (variant)
                     /note="color: #a6acb3; direction: RIGHT"
     gene            1527..2240
                     /gene="ECFP (variant)"
                     /label=ECFP (variant)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(1569..1590)
                     /label=EGFP_N_primer
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(2083..2102)
                     /label=(534) GFP seq 3
                     /note="color: black; sequence: GGGGCCGTCGCCGATGGGGG"
     misc_feature    2142..2144
                     /label=A206K
                     /note="color: #ff0000"
     misc_feature    2177..2198
                     /label=EGFP_C_primer
                     /note="color: #a6acb3"
     polyA_signal    2361..2482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(2489..2944)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        2971..3075
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3077..3434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      3285..3420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             3469..4263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     3926..3945
                     /label=(877) KanNeoR F3
                     /note="color: black; sequence: tcgatcaggatgatctggac"
     polyA_signal    4499..4517
                     /label=TK polyA
                     /note="color: #a6acb3"
     misc_feature    4513..4538
                     /label=(966)_HSV-TK-polyA_3'_BamHI_KpnI
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     4538..4563
                     /label=oTK441
                     /note="color: black; sequence: 
                     CTACGAaaGaGGTGTCGCAGTCTTGTGCACAGAGggatccACTTTTCTAGCTGCCCCTT
                     T"
     misc_feature    4539..5581
                     /label=DHC1 homology
                     /note="color: #ff99cc"
     CDS             4539..4543
                     /codon_start=1
                     /label=Linker
                     /note="color: #ffcc99"
                     /translation="S"
     primer_bind     complement(5557..5587)
                     /label=oTK426
                     /note="color: black; sequence: 
                     gcgcgcctcgagTGGGCCACTGTCATCATCACCACTG"
     promoter        complement(5613..5631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5638..5654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      5795..6223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     CDS             6567..7361
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     7024..7043
                     /label=(877) KanNeoR F3
                     /note="color: black; sequence: tcgatcaggatgatctggac"
     CDS             7379..8239
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7379 .. 7447 / #ccffcc / signal sequence
                        2: 7448 .. 8239 / #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8410..8998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttcag cgagaggatc aaacagctgc agaacatctc actggcagct gcatctggtg
      301 gcgccaagga gctaaaggtg aaggcgctcc tgacgagtct cgggtggtca gcagctgtcc
      361 tgggctgggg tgggagtggc tctggggaaa aacacagggc ccaggtctga cctgagctcc
      421 ttcccctggg ggctgctgct ttccacagaa catccacgtg tgcctgggtg gcctgttcgt
      481 gcctgaggcg tacatcactg ccaccaggca gtatgtggcc caggccaaca gctggtccct
      541 ggaggagctc tgcctggaag tcaacgtcac cacctcacag ggcgccaccc ttgacgcttg
      601 cagcttcgga gtcacgggtg agtggagtct cacagaaaat actggctctt tgcaggtgac
      661 ctcggtggcc tgagaccatt gttcccagat acatgcactt agggtgaccg gctggcagtt
      721 gggtggagcc tctgggcgcc ctgtgactgg ggtttgtgta gctgttgtgt tcacctcagc
      781 ctgggttttg gcttccgcct cacaggtttg aaacttcaag gggccacgtg caacaacaac
      841 aagctgtcac tgtccaatgc catctcaacc gcccttcccc tgacgcagct gcgctgggtc
      901 aagcagacaa acaccgagaa gaaggccagt gtggtaagga ggcactgcct ttcccaggca
      961 ttctgcaggg acccctgcgg taacaagggc agaggcggct cctcttctat gcctgggttc
     1021 cacttggaac gggaaatgag gttcacagag gaaatccatt tcgcacctgt ccaaacctct
     1081 ccttgccggg ccccatcagc tgtcccgggc agtcttccag ttttcttact tttcccttaa
     1141 gccaccagta aacccctctg cttctgcagg taaccttacc tgtctacctg aacttcaccc
     1201 gtgcagacct catcttcacc gtggacttcg aaattgctac aaaggaggac cctcgctcgt
     1261 tctacgaaag aggtgtcgca gtcttgtgca cagagggatc cggtgcaggc gccggattca
     1321 gcgagaccgt agacctcatg ctcaatttgc agagcaataa ggagggtagc gttgacctga
     1381 aaaatgtgtc agccgtgccg aaagaaaaga cgacattgaa ggacccctcc aagccccctg
     1441 caaaagcaca agtagtcgga tggccacccg tgcggaacta caggaagaat atgatgaccc
     1501 agcagaaaac ttcatccgct agcatggtga gcaagggcga ggagctgttc accggggtgg
     1561 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg
     1621 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
     1681 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca
     1741 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1801 acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg
     1861 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1921 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata
     1981 tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg
     2041 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2101 ccgtgctgct gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca
     2161 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg
     2221 gcatggacga gctgtacaag taatctagat aactgatcat aatcagccat accacatttg
     2281 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2341 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2461 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     2521 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2581 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2641 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2701 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2761 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2821 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2881 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2941 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3061 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3181 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3241 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3361 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3421 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3481 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     3541 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3601 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     3661 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     3721 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     3781 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     3841 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     3901 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     3961 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4021 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4081 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4141 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4201 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4261 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4321 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4381 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     4441 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4501 taaaaagaca gaataaaacg cacggtgttg ggtcgttgga tccacttttc tagctgcccc
     4561 tttctgtaat agtgaaagtt ggtatttaac atttattcat ttttaaaata tttggaaggt
     4621 ctgagcttgt gaaaagaaag tggttggtct gaggttggag gaagctgaat ggaatctgac
     4681 ggttgggagt ggtggaaatt ggaaggatac caggaggtat ttgggaaggc caatggcgtg
     4741 gctcctttga ggaaataaaa cactaagcat gagccggctc cgcctcttct gtctccgctt
     4801 tcatcccagg gcacagagcc ttgccttcca tgctgcccag ggagggcagc ccacggcagc
     4861 catgcccctc cccacctcgc tttcatcatg agctcgctcc cgagcggcca cagcactcat
     4921 gaatgaagac cttggggccc ttcacagaca cagatgcagc cagctgtggc tctgaaggcc
     4981 ctggggcccg ggcaccatgg ttcacacctt taatcgcagc actttgggag tctgggagtt
     5041 aaagaccagc ctcggcaaca tagtgagacc ccgtctctac aggaaattaa atcaggtgtg
     5101 gtggtgcatg cctgtagtcc cagctacttg agggctgagg tgggaggatc acccaagccc
     5161 aagaggtcga ggctgcagtg agctgtgatc tcaccactgc actccagcct gggtgacaga
     5221 gcaagaccct gtctcaaaaa aaaaaaaagc tgggcgtggt ggctcatgcc tgtaatccca
     5281 gcactttggg aggccgaggc gggcggatca ccaaattagc cgggcatggt ggcacatgcc
     5341 tgtaatccca gctactcggg aggctgaggc aggagaattg cttgaacctg ggaggcggag
     5401 gttgcagtga gctgaaaaaa aaaaaggcag cccccagccg cttgtgttct tgaccagggc
     5461 cccaggactt ggctcctcca gacaagggag ttttgtgctg tagatgaggg agttgcccat
     5521 cgccgcccta gcaagtccat tcccacacga cctttccagt ggtgatgatg acagtggccc
     5581 actcgagcat gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact
     5641 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5701 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5761 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     5821 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5881 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5941 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6001 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6061 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6121 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6181 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aattcagggc
     6241 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac
     6301 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
     6361 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
     6421 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
     6481 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc
     6541 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6601 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6661 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6721 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6781 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6841 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct cctgccgaga
     6901 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6961 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7021 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7081 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7141 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7201 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7261 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7321 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattgaaaaa ggaagagtat
     7381 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7441 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7501 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7561 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7621 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7681 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7741 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7801 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7861 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7921 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7981 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8041 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8101 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8161 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8221 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8401 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8461 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8521 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8581 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8641 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8701 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8761 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8821 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8881 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8941 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9001 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9061 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9121 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
