LOCUS       Exported                6541 bp ds-DNA     circular UNA 30-AUG-2019
DEFINITION  natural circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hsyn-GRAB5HT2.1(seq)
SOURCE      natural DNA sequence (unknown)
  ORGANISM  natural DNA sequence (unknown)
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="L-ITR"
     promoter        156..597
                     /note="synapsin"
     gene            598..2655
                     /label=GRAB-HT
                     /label=GRAB-5-HT1.0
                     /note="GRAB-5HT2.1"
     misc_feature    1438..2187
                     /label=cpEGFP
                     /note="cpEGFP"
     unsure          2658..3266
                     /label=WPRE
                     /note="WPRE"
     polyA_signal    3286..3764
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="hGH polyA"
     unsure          3804..3944
                     /label=R-ITR
                     /note="R-ITR"
     rep_origin      4036..4342
                     /label=f1 Origin
                     /label=Replication origin
                     /note="f1 Origin"
     unsure          4861..5718
                     /label=AmpR
                     /note="AmpR"
     rep_origin      5869..6536
                     /label=pUC ori
                     /label=Replication origin
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtgtgaacc tgaggaatgc ggtgcattca
      721 ttccttgtgc acctaattgg cctattggtt tggcaatgtg atatttctgt gagcccagta
      781 gcagctatag taactgacat tttcaatacc tccgatggtg gacgcttcaa attcccagac
      841 ggggtacaaa actggccagc actttcaatc gtcatcataa taatcatgac aataggtggc
      901 aacatccttg tgatcatggc agtaagcatg gaaaagaaac tgcacaatgc caccaattac
      961 ttcttaatgt ccctagccat tgctgatatg ctagtgggac tacttgtcat gcccctgtct
     1021 ctcctggcaa tcctttatga ttatgtctgg ccactaccta gatatttgtg ccccgtctgg
     1081 atttctttag atgttttatt ttcaacagcg tccatcatgc acctctgcgc tatatcgctg
     1141 gatcggtatg tagcaatacg taatcctatt gagcatagcc gtttcaattc gcggactaag
     1201 gccatcatga agattgctat tgtttgggca atttctatag gtgtatcagt tcctatccct
     1261 gtgattggac tgagggacga agaaaaggtg ttcgtgaaca acacgacgtg cgtgctcaac
     1321 gacccaaatt tcgttcttat tgggtccttc gtagctttct tcataccgct gacgattatg
     1381 gtgattacgt attgcctgac catctacgtt ctgcgccgac aagctttgat gtttctgaac
     1441 ggaaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa cttccacatc
     1501 cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca gaacaccccc
     1561 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca gtccaaactt
     1621 tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1681 ggaatcactc tcggcatgga cgagctgtac aagggcggta ccggagggca agaatccatg
     1741 agcagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1801 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc caccactggc
     1861 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1921 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1981 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
     2041 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2101 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2161 ctggagtaca acggatttgc aacagcaaac aatgaaagaa aagcttcgaa agtccttggg
     2221 attgttttct ttgtgtttct gatcatgtgg tgcccatttt tcattaccaa tattctgtct
     2281 gttctttgtg agaagtcctg taaccaaaag ctcatggaaa agcttctgaa tgtgtttgtt
     2341 tggattggct atgtttgttc aggaatcaat cctctggtgt atactctgtt caacaaaatt
     2401 taccgaaggg cattctccaa ctatttgcgt tgcaattata aggtagagaa aaagcctcct
     2461 gtcaggcaga ttccaagagt tgccgccact gctttgtctg ggagggagct taatgttaac
     2521 atttatcggc ataccaatga accggtgatc gagaaagcca gtgacaatga gcccggtata
     2581 gagatgcaag ttgagaattt agagttacca gtaaatccct ccagtgtggt tagcgaaagg
     2641 attagcagtg tgtgaaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     2701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3061 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     3121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3241 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     3301 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     3361 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     3421 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3481 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3541 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     3601 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     3661 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     3721 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     3781 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3841 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3901 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3961 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4021 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4081 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4141 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4201 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4321 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     4381 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4441 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4501 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4561 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4621 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4681 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5401 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5701 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6001 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6541 t
//
