LOCUS       Exported                6352 bp ds-DNA     circular UNA 24-FEB-2020
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=synapsin
     unsure          598..2454
                     /label=IgK-DA4.3M205T
     CDS             598..2454
                     /codon_start=1
                     /label=GRAB_DA2m
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGDPLNLSWYDDDLE
                     RQNWSRPFNGSDGKADRPHYNYYATLLTLLIAVIVFGNVLVCMAVSREKALQTTTNYLI
                     VSLAVADLLVATLVMPWVVYLEVVGEWKFSRIHCDIFVTLDVMMCTASILNLCAISIDR
                     YTAVAMPMLYNTRYSSKRRVTVMISIVWVLSFTISCPLLFGLNNADQNECIIANPAFVV
                     YSSIVSFYVPFIVTLLVYIKIYIVLRRRRKRVNTKRSSRAFRAHLRAPLKGNCTHPEDM
                     KLGGTVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSV
                     QSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGKSMVSKGEELFTGVVPILV
                     ELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYP
                     DHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDG
                     NILGHKLEYNTGAAAMSRRKLSQQKEKKATQMLAIVLGVFIICWLPFFITHILNIHCDC
                     NIPPVLYSAFTWLGYVNSAVNPIIYTTFNIEFRKAFLKILHC"
     unsure          2469..3077
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3097..3575
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          3615..3755
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      3847..4153
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          4672..5529
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5680..6347
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtgatccac tgaatctgtc ctggtatgat
      721 gatgatctgg agaggcagaa ctggagccgg cccttcaacg ggtcagacgg gaaggcggac
      781 agaccccact acaactacta tgccacactg ctcaccctgc tcatcgctgt catcgtcttc
      841 ggcaacgtgc tggtgtgcat ggctgtgtcc cgcgagaagg cgctgcagac caccaccaac
      901 tacctgatcg tcagcctcgc agtggccgac ctcctcgtcg ccacactggt catgccctgg
      961 gttgtctacc tggaggtggt aggtgagtgg aaattcagca ggattcactg tgacatcttc
     1021 gtcactctgg acgtcatgat gtgcacggcg agcatcctga acttgtgtgc catcagcatc
     1081 gacaggtaca cagctgtggc catgcccatg ctgtacaata cgcgctacag ctccaagcgc
     1141 cgggtcaccg tcatgatctc catcgtctgg gtcctgtcct tcaccatctc ctgcccactc
     1201 ctcttcggac tcaataacgc agaccagaac gagtgcatca ttgccaaccc ggccttcgtg
     1261 gtctactcct ccatcgtctc cttctacgtg cccttcattg tcaccctgct ggtctacatc
     1321 aagatctaca ttgtcctccg cagacgccgc aagcgagtca acaccaaacg cagcagccga
     1381 gctttcaggg cccacctgag ggctccacta aagggcaact gtactcaccc cgaggacatg
     1441 aaactcggag gaactgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
     1501 ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     1561 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
     1621 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1681 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggaaa
     1741 tccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1801 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     1861 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1921 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1981 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     2041 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2101 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2161 cacaagctgg agtacaacac tggagcagca gcaatgagcc gtaggaagct ctcccagcag
     2221 aaggagaaga aagccactca gatgctcgcc attgttctcg gcgtgttcat catctgctgg
     2281 ctgcccttct tcatcacaca catcctgaac atacactgtg actgcaacat cccgcctgtc
     2341 ctgtacagcg ccttcacgtg gctgggctat gtcaacagcg ccgtgaaccc catcatctac
     2401 accaccttca acattgagtt ccgcaaggcc ttcctgaaga tcctccactg ctgagaattc
     2461 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2521 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2581 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2641 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2701 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2761 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2821 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2881 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     2941 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3001 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3061 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     3121 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     3181 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     3241 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     3301 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     3361 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     3421 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     3481 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     3541 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     3601 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3661 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     3721 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     3781 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     3841 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3901 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3961 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4021 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4081 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4141 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     4201 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4261 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     4321 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4381 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4441 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4501 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5821 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
