LOCUS       Exported                6235 bp ds-DNA     circular UNA 24-FEB-2020
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=synapsin
     CDS             598..2337
                     /codon_start=1
                     /label=GRAB_rDA1h
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGDPLNLSWYDDDLE
                     RQNWSRPFNGSDGKADRPHYNYYATLLTLLIAVIVFGNVLVCMAVSREKALQTTTNYLI
                     VSLAVADLLVATLVMPWVVYLEVVGEWKFSRIHCDIFVTLDVMMCTASILNLCAISIDR
                     YTAVAMPMLYNTRYSSKRRVTVMISIVWVLSFTISCPLLFGLNNADQNECIIANPAFVV
                     YSSIVSFYVPFIVMLLVYIKIYIVLRRRRKRVPVCSEVMYPEDGALKSEIKKGLRLKDG
                     GHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEARHSTGGMDEL
                     YKGGTGGSLVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKL
                     KVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGII
                     HVNQDSSLQDGYFIYKVKLRGTNFPPDGPVMQKKTMGWELTVQQLEKKATQMLAIVLGV
                     FIICWLPFFITHILNIHCDCNIPPVLYSAFTWLGYVNSAVNPIIYTTFNIEFRKAFLKI
                     LHC"
     gene            1303..1305
                     /label=205
     gene            2104..2106
                     /label=6.29
     unsure          2335..2337
                     /label=IgK-DA4.1
     unsure          2352..2960
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    2980..3458
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          3498..3638
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      3730..4036
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          4555..5412
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5563..6230
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtgatccac tgaatctgtc ctggtatgat
      721 gatgatctgg agaggcagaa ctggagccgg cccttcaacg ggtcagacgg gaaggcggac
      781 agaccccact acaactacta tgccacactg ctcaccctgc tcatcgctgt catcgtcttc
      841 ggcaacgtgc tggtgtgcat ggctgtgtcc cgcgagaagg cgctgcagac caccaccaac
      901 tacctgatcg tcagcctcgc agtggccgac ctcctcgtcg ccacactggt catgccctgg
      961 gttgtctacc tggaggtggt aggtgagtgg aaattcagca ggattcactg tgacatcttc
     1021 gtcactctgg acgtcatgat gtgcacggcg agcatcctga acttgtgtgc catcagcatc
     1081 gacaggtaca cagctgtggc catgcccatg ctgtacaata cgcgctacag ctccaagcgc
     1141 cgggtcaccg tcatgatctc catcgtctgg gtcctgtcct tcaccatctc ctgcccactc
     1201 ctcttcggac tcaataacgc agaccagaac gagtgcatca ttgccaaccc ggccttcgtg
     1261 gtctactcct ccatcgtctc cttctacgtg cccttcattg tcatgctgct ggtctacatc
     1321 aagatctaca ttgtcctccg cagacgccgc aagcgagtcc ccgtgtgttc cgaggttatg
     1381 taccccgagg acggcgccct gaagagcgag atcaagaagg ggctgaggct gaaggacggc
     1441 ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1501 ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga ctacaccatc
     1561 gtggaacagt gcgaacgcgc cgaggcccgc cactccaccg gcggcatgga cgagctgtac
     1621 aagggaggta caggcgggag tctggtgagc aagggcgagg aggataacat ggccatcatc
     1681 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     1741 atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg
     1801 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     1861 tccaaggcct acattaagca cccagccgac atccccgact acttcaagct gtccttcccc
     1921 gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac
     1981 caggactcct ccctgcagga cggctacttc atctacaagg tgaagctgcg cggcaccaac
     2041 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggagct gacggtccag
     2101 cagctcgaga agaaagccac tcagatgctc gccattgttc tcggcgtgtt catcatctgc
     2161 tggctgccct tcttcatcac acacatcctg aacatacact gtgactgcaa catcccgcct
     2221 gtcctgtaca gcgccttcac gtggctgggc tatgtcaaca gcgccgtgaa ccccatcatc
     2281 tacaccacct tcaacattga gttccgcaag gccttcctga agatcctcca ctgctgagaa
     2341 ttcgatatca agcttatcga tcatcaacct ctggattaca aaatttgtga aagattgact
     2401 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2461 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2521 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2581 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2641 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2701 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2761 tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc
     2821 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2881 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2941 gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc
     3001 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     3061 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     3121 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     3181 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     3241 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     3301 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     3361 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     3421 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt
     3481 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3541 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3601 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     3661 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     3721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3901 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     3961 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4021 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     4081 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4141 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     4201 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4261 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4321 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     4381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
