LOCUS       pX165_eSpCas9           8214 bp ds-DNA     circular     24-FEB-2020
DEFINITION  Human codon optimized Cas9 nuclease from Streptococcus pyogenes
            (Cbh-3X-FLAG-NLS-SpCas9-NLS).
ACCESSION   .
VERSION     .
KEYWORDS    pSpCas9 (PX165)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143.
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(8096..8115)
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /locus_tag="pRS-marker"
                     /label="pRS-marker"
                     /ApEinfo_label="pRS-marker"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7624..8079
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7921..7942
                     /note="F1 origin, forward primer"
                     /locus_tag="F1ori-F"
                     /label="F1ori-F"
                     /ApEinfo_label="F1ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7711..7730)
                     /note="F1 origin, reverse primer"
                     /locus_tag="F1ori-R"
                     /label="F1ori-R"
                     /ApEinfo_label="F1ori-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_region   complement(7409..7549)
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /locus_tag="AAV2 ITR"
                     /label="AAV2 ITR"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_region   complement(7409..7538)
                     /locus_tag="AAV2 ITR(1)"
                     /label="AAV2 ITR(1)"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    complement(7193..7400)
                     /note="bovine growth hormone polyadenylation signal"
                     /locus_tag="bGH poly(A) signal"
                     /label="bGH poly(A) signal"
                     /ApEinfo_label="bGH poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7187..7204)
                     /note="Bovine growth hormone terminator, reverse primer.
                     Also called BGH reverse"
                     /locus_tag="BGH-rev"
                     /label="BGH-rev"
                     /ApEinfo_label="BGH-rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7112..7159
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /translation="KRPAATKKAGQAKKKK"
                     /locus_tag="nucleoplasmin NLS"
                     /label="nucleoplasmin NLS"
                     /ApEinfo_label="nucleoplasmin NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2966..2986
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2894..2959
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /locus_tag="3xFLAG"
                     /label="3xFLAG"
                     /ApEinfo_label="3xFLAG"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     regulatory      complement(2885..2894)
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /locus_tag="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /label="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /ApEinfo_label="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          complement(2646..2873)
                     /note="hybrid between chicken beta-actin (CBA) and minute
                     virus of mice (MMV) introns (Gray et al., 2011)"
                     /locus_tag="hybrid intron"
                     /label="hybrid intron"
                     /ApEinfo_label="hybrid intron"
                     /ApEinfo_fwdcolor="#eb606c"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2368..2645
                     /locus_tag="chicken beta-actin promoter"
                     /label="chicken beta-actin promoter"
                     /ApEinfo_label="chicken beta-actin promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        complement(2081..2366)
                     /note="human cytomegalovirus immediate early enhancer;
                     contains an 18-bp deletion relative to the standard CMV
                     enhancer"
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_region   complement(1933..2062)
                     /note="Functional equivalent of wild-type AAV2 ITR"
                     /locus_tag="AAV2 ITR (alternate)"
                     /label="AAV2 ITR (alternate)"
                     /ApEinfo_label="AAV2 ITR (alternate)"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1283..1871
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1772..1791
                     /note="pBR322 origin, forward primer"
                     /locus_tag="pBR322ori-F"
                     /label="pBR322ori-F"
                     /ApEinfo_label="pBR322ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             252..1112
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(470..489)
                     /note="Ampicillin resistance gene, reverse primer"
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        147..251
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(61..79)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
                     /locus_tag="pBRforEco"
                     /label="pBRforEco"
                     /ApEinfo_label="pBRforEco"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1..23
                     /note="pGEX vectors, reverse primer"
                     /locus_tag="pGEX 3'"
                     /label="pGEX 3'"
                     /ApEinfo_label="pGEX 3'"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2891..2893
                     /locus_tag="START"
                     /label="START"
                     /ApEinfo_label="START"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7160..7162
                     /locus_tag="STOP"
                     /label="STOP"
                     /ApEinfo_label="STOP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3011..7111
                     /locus_tag="Sniper Cas9"
                     /label="Sniper Cas9"
                     /ApEinfo_label="Sniper Cas9"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
       61 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      121 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      181 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      241 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      301 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      361 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      421 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      481 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      541 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      601 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      661 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      721 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      781 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      841 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      901 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      961 agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1021 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1081 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1141 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1201 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1861 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1921 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
     1981 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
     2041 aactccatca ctaggggttc ctgcggcctc tagaggtacc cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa
     2161 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2221 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2281 ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2341 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     2401 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     2461 cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
     2521 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     2581 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     2641 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     2701 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     2761 ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg
     2821 gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac
     2881 cggtgccacc atggactata aggaccacga cggagactac aaggatcatg atattgatta
     2941 caaagacgat gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt
     3001 cccagcagcc GACAAGAAGT ACAGCATCGG CCTGGACATC GGCACCAACT CTGTGGGCTG
     3061 GGCCGTGATC ACCGACGAGT ACAAGGTGCC CAGCAAGAAA TTCAAGGTGC TGGGCAACAC
     3121 CGACCGGCAC AGCATCAAGA AGAACCTGAT CGGAGCCCTG CTGTTCGACA GCGGCGAAAC
     3181 AGCCGAGGCC ACCCGGCTGA AGAGAACCGC CAGAAGAAGA TACACCAGAC GGAAGAACCG
     3241 GATCTGCTAT CTGCAAGAGA TCTTCAGCAA CGAGATGGCC AAGGTGGACG ACAGCTTCTT
     3301 CCACAGACTG GAAGAGTCCT TCCTGGTGGA AGAGGATAAG AAGCACGAGC GGCACCCCAT
     3361 CTTCGGCAAC ATCGTGGACG AGGTGGCCTA CCACGAGAAG TACCCCACCA TCTACCACCT
     3421 GAGAAAGAAA CTGGTGGACA GCACCGACAA GGCCGACCTG CGGCTGATCT ATCTGGCCCT
     3481 GGCCCACATG ATCAAGTTCC GGGGCCACTT CCTGATCGAG GGCGACCTGA ACCCCGACAA
     3541 CAGCGACGTG GACAAGCTGT TCATCCAGCT GGTGCAGACC TACAACCAGC TGTTCGAGGA
     3601 AAACCCCATC AACGCCAGCG GCGTGGACGC CAAGGCCATC CTGTCTGCCA GACTGAGCAA
     3661 GAGCAGACGG CTGGAAAATC TGATCGCCCA GCTGCCCGGC GAGAAGAAGA ATGGCCTGTT
     3721 CGGAAACCTG ATTGCCCTGA GCCTGGGCCT GACCCCCAAC TTCAAGAGCA ACTTCGACCT
     3781 GGCCGAGGAT GCCAAACTGC AGCTGAGCAA GGACACCTAC GACGACGACC TGGACAACCT
     3841 GCTGGCCCAG ATCGGCGACC AGTACGCCGA CCTGTTTCTG GCCGCCAAGA ACCTGTCCGA
     3901 CGCCATCCTG CTGAGCGACA TCCTGAGAGT GAACACCGAG ATCACCAAGG CCCCCCTGAG
     3961 CGCCTCTATG ATCAAGAGAT ACGACGAGCA CCACCAGGAC CTGACCCTGC TGAAAGCTCT
     4021 CGTGCGGCAG CAGCTGCCTG AGAAGTACAA AGAGATTTTC TTCGACCAGA GCAAGAACGG
     4081 CTACGCCGGC TACATTGACG GCGGAGCCAG CCAGGAAGAG TTCTACAAGT TCATCAAGCC
     4141 CATCCTGGAA AAGATGGACG GCACCGAGGA ACTGCTCGTG AAGCTGAACA GAGAGGACCT
     4201 GCTGCGGAAG CAGCGGACCT TCGACAACGG CAGCATCCCC CACCAGATCC ACCTGGGAGA
     4261 GCTGCACGCC ATTCTGCGGC GGCAGGAAGA TTTTTACCCA TTCCTGAAGG ACAACCGGGA
     4321 AAAGATCGAG AAGATCCTGA CCTTCCGCAT CCCCTACTAC GTGGGCCCTC TGGCCAGGGG
     4381 AAACAGCAGA TTCGCCTGGA TGACCAGAAA GAGCGAGGAA ACCATCACCC CCTGGAACTT
     4441 CGAGGAAGTG GTGGACAAGG GCGCTTCCGC CCAGAGCTTC ATCGAGCGGA TGACCAACTT
     4501 CGATAAGAAC CTGCCCAACG AGAAGGTGCT GCCCAAGCAC AGCCTGCTGT ACGAGTACTT
     4561 CACCGTGTAT AACGAGCTGA CCAAAGTGAA ATACGTGACC GAGGGAATGA GAAAGCCCGC
     4621 CAGCCTGAGC GGCGAGCAGA AAAAGGCCAT CGTGGACCTG CTGTTCAAGA CCAACCGGAA
     4681 AGTGACCGTG AAGCAGCTGA AAGAGGACTA CTTCAAGAAA ATCGAGTGCT TCGACTCCGT
     4741 GGAAATCTCC GGCGTGGAAG ATCGGTTCAA CGCCTCCCTG GGCACATACC ACGATCTGCT
     4801 GAAAATTATC AAGGACAAGG ACTTCCTGGA CAATGAGGAA AACGAGGACA TTCTGGAAGA
     4861 TATCGTGCTG ACCCTGACAC TGTTTGAGGA CAGAGAGATG ATCGAGGAAC GGCTGAAAAC
     4921 CTATGCCCAC CTGTTCGACG ACAAAGTGAT GAAGCAGCTG AAGCGGCGGA GATACACCGG
     4981 CTGGGGCAGG CTGAGCCGGA AGCTGATCAA CGGCATCCGG GACAAGCAGT CCGGCAAGAC
     5041 AATCCTGGAT TTCCTGAAGT CCGACGGCTT CGCCAACAGA AACTTCATGC AGCTGATCCA
     5101 CGACGACAGC CTGACCTTTA AAGAGGACAT CCAGAAAGCC CAGGTGTCCG GCCAGGGCGA
     5161 TAGCCTGCAC GAGCACATTG CCAATCTGGC CGGCAGCCCC GCCATTAAGA AGGGCATCCT
     5221 GCAGACAGTG AAGGTGGTGG ACGAGCTCGT GAAAGTGATG GGCCGGCACA AGCCCGAGAA
     5281 CATCGTGATC GAAATCGCCA GAGAGAACCA GACCACCCAG AAGGGACAGA AGAACAGCCG
     5341 CGAGAGAATG AAGCGGATCG AAGAGGGCAT CAAAGAGCTG GGCAGCCAGA TCCTGAAAGA
     5401 ACACCCCGTG GAAAACACCC AGCTGCAGAA CGAGAAGCTG TACCTGTACT ACCTGCAGAA
     5461 TGGGCGGGAT ATGTACGTGG ACCAGGAACT GGACATCAAC CGGCTGTCCG ACTACGATGT
     5521 GGACCATATC GTGCCTCAGA GCTTTCTGAA GGACGACTCC ATCGACAACA AGGTGCTGAC
     5581 CAGAAGCGAC AAGAACCGGG GCAAGAGCGA CAACGTGCCC TCCGAAGAGG TCGTGAAGAA
     5641 GATGAAGAAC TACTGGCGGC AGCTGCTGAA CGCCAATCTG ATTACCCAGA GAAAGTTCGA
     5701 CAATCTGACC AAGGCCGAGA GAGGCGGCCT GAGCGAACTG GATAAGGCCG GCTTCATCAA
     5761 GAGACAGCTG GTGGAAACCC GGCAGATCAC AAAGCACGTG GCACAGATCC TGGACTCCCG
     5821 GATGAACACT AAGTACGACG AGAATGACAA GCTGATCCGG GAAGTGAAAG TGATCACCCT
     5881 GAAGTCCAAG CTGGTGTCCG ATTTCCGGAA GGATTTCCAG TTTTACAAAG TGCGCGAGAT
     5941 CAACAACTAC CACCACGCCC ACGACGCCTA CCTGAACGCC GTCGTGGGAA CCGCCCTGAT
     6001 CAAAAAGTAC CCTAAGCTGG AAAGCGAGTT CGTGTACGGC GACTACAAGG TGTACGACGT
     6061 GCGGAAGATG ATCGCCAAGA GCGAGCAGGA AATCGGCAAG GCTACCGCCA AGTACTTCTT
     6121 CTACAGCAAC ATCATGAACT TTTTCAAGAC CGAGATTACC CTGGCCAACG GCGAGATCCG
     6181 GAAGCGGCCT CTGATCGAGA CAAACGGCGA AACCGGGGAG ATCGTGTGGG ATAAGGGCCG
     6241 GGATTTTGCC ACCGTGCGGA AAGTGCTGAG CATGCCCCAA GTGAATATCG TGAAAAAGAC
     6301 CGAGGTGCAG ACAGGCGGCT TCAGCAAAGA GTCTATCCTG CCCAAGAGGA ACAGCGATAA
     6361 GCTGATCGCC AGAAAGAAGG ACTGGGACCC TAAGAAGTAC GGCGGCTTCG ACAGCCCCAC
     6421 CGTGGCCTAT TCTGTGCTGG TGGTGGCCAA AGTGGAAAAG GGCAAGTCCA AGAAACTGAA
     6481 GAGTGTGAAA GAGCTGCTGG GGATCACCAT CATGGAAAGA AGCAGCTTCG AGAAGAATCC
     6541 CATCGACTTT CTGGAAGCCA AGGGCTACAA AGAAGTGAAA AAGGACCTGA TCATCAAGCT
     6601 GCCTAAGTAC TCCCTGTTCG AGCTGGAAAA CGGCCGGAAG AGAATGCTGG CCTCTGCCGG
     6661 CGAACTGCAG AAGGGAAACG AACTGGCCCT GCCCTCCAAA TATGTGAACT TCCTGTACCT
     6721 GGCCAGCCAC TATGAGAAGC TGAAGGGCTC CCCCGAGGAT AATGAGCAGA AACAGCTGTT
     6781 TGTGGAACAG CACAAGCACT ACCTGGACGA GATCATCGAG CAGATCAGCG AGTTCTCCAA
     6841 GAGAGTGATC CTGGCCGACG CTAATCTGGA CAAAGTGCTG TCCGCCTACA ACAAGCACCG
     6901 GGATAAGCCC ATCAGAGAGC AGGCCGAGAA TATCATCCAC CTGTTTACCC TGACCAATCT
     6961 GGGAGCCCCT GCCGCCTTCA AGTACTTTGA CACCACCATC GACCGGAAGA GGTACACCAG
     7021 CACCAAAGAG GTGCTGGACG CCACCCTGAT CCACCAGAGC ATCACCGGCC TGTACGAGAC
     7081 ACGGATCGAC CTGTCTCAGC TGGGAGGCGA Caaaaggccg gcggccacga aaaaggccgg
     7141 ccaggcaaaa aagaaaaagt aagaattcct agagctcgct gatcagcctc gactgtgcct
     7201 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7261 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7321 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag
     7381 aatagcaggc atgctgggga gcggccgcag gaacccctag tgatggagtt ggccactccc
     7441 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     7501 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     7561 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     7621 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7681 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7801 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     7861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7921 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     7981 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     8041 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     8101 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8161 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtct
//
