LOCUS       Exported                4133 bp ds-DNA     circular SYN 23-FEB-2020
DEFINITION  Kanamycin resistance donor for ShCAST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4133)
  TITLE     RNA-guided DNA insertion with CRISPR-associated transposases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..1824
                     /organism="Red recombinase plasmid pKD46"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:170495"
     primer_bind     1..29
                     /label=pSC101-F
     CDS             complement(47..997)
                     /codon_start=1
                     /transl_table=11
                     /product="repA101 protein"
                     /label=repA101
                     /db_xref="GI:15554350"
                     /protein_id="AAL02049.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     complement(230..267)
                     /label=donor-check-R
     rep_origin      1001..1741
                     /label=oriR101
                     /note="oriR101"
     primer_bind     complement(1797..1824)
                     /label=pSC101-R
     primer_bind     1805..1841
                     /label=donor-shCAST-F
     LTR             1884..2093
                     /label=LE
     protein_bind    complement(2149..2196)
                     /label=FRT
     CDS             2583..3377
                     /codon_start=1
                     /label=NeoR/KanR
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(3485..3532)
                     /label=FRT
     terminator      3580..3666
                     /label=rrnB T1 terminator
     terminator      3769..3863
                     /label=lambda t0 terminator
     CDS             complement(3882..4093)
                     /label=RE
     primer_bind     3911..3951
                     /label=donor-check-F
     primer_bind     complement(join(4115..4133,1..20))
                     /label=donor-shCAST-R
ORIGIN
        1 tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt
       61 atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac
      121 cattgagatc atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct
      181 gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttacgt aggtaggaat
      241 ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt
      301 cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc
      361 ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg
      421 gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca
      481 tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta
      541 gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat
      601 gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac
      661 taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct
      721 caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg
      781 ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt
      841 tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta
      901 gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg
      961 ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat
     1021 tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt
     1081 gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta
     1141 gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta
     1201 taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt
     1261 ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg
     1321 ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg
     1381 ttgcggccgc aatcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     1441 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     1501 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     1561 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     1621 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     1681 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     1741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1801 caaaaaggat cttcacctag atccgttatg cagcggaaag taaaaaattt ttagtttatt
     1861 agacatctcc acaaaaggcg tagtgtacag tgacaaatta tctgtcgtcg gtgacagatt
     1921 aatgtcattg tgactattta attgtcgtcg tgacccatca gcgttgctta attaattgat
     1981 gacaaattaa atgtcatcaa tataatatgc tctgcaatta ttatacaaag caattaaaac
     2041 aagcggataa aaggacttgc tttcaaccca cccctaagtt taatagttac tgagggggat
     2101 ccactagtga gctcatgcat gatctcgaat tagcttcaaa agcgctctga agttcctata
     2161 ctttctagag aataggaact tcggaatagg aacttcaaga tcccctgatt ccctttgtca
     2221 acagcaatgg ataattcgat ttaacaaatg catggcgcaa gggctgctaa aggaagcgga
     2281 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     2341 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     2401 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     2461 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     2521 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     2581 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     2641 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     2701 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     2761 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     2821 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     2881 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     2941 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3001 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3061 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3121 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3181 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3241 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3301 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3361 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagg atccaacatt tccaatcact
     3421 agtgaattat ctagaattat tccattgagt aagtttttaa gcacatcagc ttcaaaagcg
     3481 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggtact tcaagatccc
     3541 caattcgaga tcgtccgggc cgcaagctcc tagcggcgga tttgtcctac tcaggagagc
     3601 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     3661 tatttgatgc ctcaagctag agagtcatta ccccaggcgt ttaagggcac caataactgc
     3721 cttaaaaaaa ttacgccccg ccctgccact catcgcagtc tagcttggat tctcaccaat
     3781 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     3841 tactggatct atcaacagga gtccaagctc agctaattaa ggcgacagtc aatttgtcat
     3901 tatgaaaata cacaaaagct ttttcctatc ttgcaaagcg acagctaatt tgtcacaatc
     3961 acggacaacg acatctattt tgtcactgca aagaggttat gctaaaactg ccaaagcgct
     4021 ataatctata ctgtataagg attttactga tgacaataat ttgtcacaac gacatataat
     4081 tagtcactgt acacgtagag acgtagcaat gctacctctc tacaatggtt ttg
//
