LOCUS       pBAV1K-lacI-Trc-mCherry 5013 bp ds-DNA     circular     06-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(1500..1519)
                     /label="LacI-R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2795..2824
                     /label="trc promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2790..2794
                     /label="5 bp upstream"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2869..3579
                     /label="mCherry"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1345..1365
                     /label="Potential end"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    2832..2848
                     /label="lac operator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      247..341
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2851..2855
                     /label="Space before BCD"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2795..2800
                     /label="-35 Box"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3564..3584
                     /label="Other end"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(357..1151)
                     /label="KanR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1357..1376
                     /label="BioBrick prefix"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      3630..3716
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2818..2823
                     /label="-10 Box"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_signal     3418..3437
                     /label="spKDL023"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_binding    2828..2850
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2833..2833
                     /label="TSS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             34..45
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1481..2563
                     /label="lacI CDS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        1403..1480
                     /label="lacIq promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ccctcatact cccttgagcc tcctccaacc gaaatagaag ggcgctgcgc ttattatttc
       61 attcagtcat cggctttcat aatctaacag acaacatctt cgctgcaaag ccacgctacg
      121 ctcaagggct tttacgctac gataacgcct gttttaacga ttatgccgat aactaaacga
      181 aataaacgct aaaacgtctc agaaacgatt ttgagacgtt ttaataaaaa atcgcctagt
      241 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
      301 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcggtactaa
      361 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
      421 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
      481 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
      541 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
      601 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
      661 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
      721 tccaattcgg ctaagcggcc gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
      781 gagtgaaaga gcctgatgca ctccgcatac agctcgatag tcttttcagg gctttgttca
      841 tcttcatacc cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
      901 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
      961 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
     1021 tttaaatata ggatttcatt ttctcccacc agcttatata ccttagcagg agacattcct
     1081 tccgtatctt ttacgcagcg gtattcttcg atcagttttt tcaattccgg tgatattctc
     1141 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
     1201 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaataca
     1261 gaaaacagcc ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     1321 gattttgaaa ccacaattat gatagaattt gacgtcgaat tcgcggccgc ttctagaact
     1381 atagctagca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc
     1441 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     1501 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     1561 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     1621 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     1681 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     1741 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     1801 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     1861 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     1921 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     1981 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     2041 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     2101 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     2161 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     2221 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     2281 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac
     2341 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     2401 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     2461 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2521 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2581 tgagttagcg cgaattgatc tggtttgaca gcttatcatc gactgcacgg tgcaccaatg
     2641 cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg
     2701 cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc
     2761 ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc cggctcgtat
     2821 aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagaccat ggtgagcaag
     2881 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt tcacatggag
     2941 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     3001 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     3061 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     3121 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3181 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaagacgg cgagttcatc
     3241 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     3301 actatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc gctgaagggc
     3361 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     3421 acctacaagg ccaagaagcc cgtgcaactg cccggcgcgt acaacgtcaa catcaagttg
     3481 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     3541 cgccactcca ccggcggcat ggacgagctg tataagtaag cctcagggcc cgatcgatgc
     3601 cgccgcttaa ttaattaatc cagaggcatc aaataaaacg aaaggctcag tcgaaagact
     3661 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     3721 cgccctagac ctagtgtcat tttatttccc ccgtttcagc atcaagaacc tttgcataac
     3781 ttgctctata tccacactga taattgccct caaaccataa tctaaaggcg ctagagtttg
     3841 ttgaaacaat atcttttaca tcattcgtat ttaaaattcc aaactccgct cccctaaggc
     3901 gaataaaagc cattaaatct tttgtattta ccaaattata gtcatccact atatctaaga
     3961 gtaaattctt caattctctt ttttggcttt catcaagtgt tatatagcgg tcaatatcaa
     4021 aatcattaat gttcaaaata tcttttttgt cgtatatatg tttattctta gcaatagcgt
     4081 cctttgattc atgagtcaaa tattcatatg aacctttgat ataatcaagt atctcaacat
     4141 gagcaactga actattcccc aattttcgct taatcttgtt cctaacgctt tctattgtta
     4201 caggatttcg tgcaatatat ataacgtgat agtgtggttt tttatagtgc tttccatttc
     4261 gtataacatc actactattc catgtatctt tatctttttt ttcgtccata tcgtgtaaag
     4321 gactgacagc catagatacg cccaaactct ctaatttttc cttccaatca ttaggaattg
     4381 agtcaggata taataaaaat ccaaaatttc tagctttagt atttttaata gccatgatat
     4441 aattacctta tcaaaaacaa gtagcgaaaa ctcgtatcct tctaaaaacg cgagctttcg
     4501 cttatttttt ttgttctgat tcctttcttg catattcttc tatagctaac gccgcaaccg
     4561 cagattttga aaaacctttt tgtttcgcca tatctgttaa ttttttatct tgctcttttg
     4621 tcagagaaat cataactctt tttttcgatt ctgaaatcac catttaaaaa actccaatca
     4681 aataatttta taaagttagt gtatcacttt gtaatcataa aaacaacaat aaagctactt
     4741 aaatatagat ttataaaaaa cgttggcgaa aacgttggcg attcgttggc gattgaaaaa
     4801 ccccttaaac ccttgagcca gttgggatag agcgtttttg gcacaaaaat tggcactcgg
     4861 cacttaatgg ggggtcgtag tacggaagca aaattcgctt cctttccccc catttttttc
     4921 caaattccaa atttttttca aaaattttcc agcgctaccg ctcggcaaaa ttgcaagcaa
     4981 tttttaaaat caaacccatg agggaatttc att
//