LOCUS       pJ2076.2_TS             4361 bp ds-DNA     circular     28-FEB-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2316..2372
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    382..396
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1156..1189
                     /label="RBS"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1091..1120
                     /label="Linker_10"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7..36
                     /label="Linker_14"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2254..2259
                     /label="SalI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1..6
                     /label="EcoRI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    93..107
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    43..57
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    729..734
                     /label="HindIII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    439..528
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2443..2450
                     /label="AscI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1050..1069
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(845..867)
                     /label="bs-1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    58..92
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2272..2277
                     /label="AatII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     oriT            3716..4351
                     /label="ColA ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    800..829
                     /label="Linker_24"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2436..2442
                     /label="BbvCI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2373..2417
                     /label="Linker_8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1085..1090
                     /label="BamHI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2308..2315
                     /label="NotI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    236..255
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2278..2307
                     /label="Linker_11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    221..235
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(153..175)
                     /label="bs-4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    176..190
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1121..1135
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    191..220
                     /label="Linker_1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2418..2423
                     /label="KasI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    913..927
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            1190..1903
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1070..1084
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    362..381
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    108..137
                     /label="Linker_21"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1993..2053
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    883..912
                     /label="Linker_19"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    403..432
                     /label="Linker_0"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2260..2271
                     /label="Spacer 5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    37..42
                     /label="SacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            complement(2791..3606)
                     /label="KanR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    785..799
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    868..882
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    529..728
                     /label="Spacer 1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1904..1969
                     /label="MarAn20"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    750..784
                     /label="P(BBa_J23102)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    138..152
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    397..402
                     /label="XhoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2054..2253
                     /label="Spacer 2.5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    433..438
                     /label="NcoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1136..1155
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    948..1049
                     /label="sgRNA-4 t4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    928..947
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1985..1992
                     /label="SbfI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2424..2435
                     /label="Spacer 15"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    830..844
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    256..361
                     /label="sgRNA-1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1970..1984
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    735..749
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 GAATTCATAG GATAGATTCT GGAAACTTTA CCGTCCGAGC TCCAGCCTGC GGTCCGGttg
       61 acggctagct cagtcctagg tacagtgcta gcTCGCTGGG ACGCCCGAGA GCCGAATCGC
      121 ACTTATTTAC AGTAGTTCAG CCTGCGGTCC GGccaTACAT ACTCTAAGAT GTGTCTCGCT
      181 GGGACGCCCG GGGACTACAC TTACGAAACT ATTGATTGCT CAGCCTGCGG TCCGGGTTCA
      241 CTGCCGTATA GGCAGTCTCA AGCTAGACTC TAGTGGTTTc AGAGCTATGC TGGAAACAGC
      301 ATAGCAAGTT gAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG
      361 CGTTCACTGC CGTATAGGCA GTCGCTGGGA CGCCCGCTCG AGCAATAAAC AGTTGATAGG
      421 GCTTCTCCGT TACCATGGTT CAGCCAAAAA ACTTAAGACC GCCGGTCTTG TCCACTACCT
      481 TGCAGTAATG CGGTGGACAG GATCGGCGGT TTTCTTTTCT CTTCTCAATT CTTCTGACCT
      541 GTAACGAATA ATAGATAGTA AAGTAGTCTC CGATTGAGTT TTCTCTGCCG AGTCCCACCC
      601 AGTTCTGTGA TTTCAGTAAG TTGGTAATTG ATACACTGTT GCGAGAACTG CTGCCTGGTA
      661 GTAGATAGGT TGTTATTGAG TAAGAAGGTA AAGTGAACGA AATCCCTGAA ACTGAGACTG
      721 TAGAAAATAA GCTTCAGCCT GCGGTCCGGT TGACAGCTAG CTCAGTCCTA GGTACTGTGC
      781 TAGCTCGCTG GGACGCCCGA GATAGCCGTT ACACAGGTGA CACTTATTTC AGCCTGCGGT
      841 CCGGccaCAC TAGAGTCTAG CTTGAGATCG CTGGGACGCC CGTGAACAGT TGCTCTGATT
      901 GAAACCACGA TTCAGCCTGC GGTCCGGGTT CACTGCCGTA TAGGCAGCAT CTTAGAGTAT
      961 GTAGTTTcAG AGCTATGCTG GAAACAGCAT AGCAAGTTgA AATAAGGCTA GTCCGTTATC
     1021 AACTTGAAAA AGTGGCACCG AGTCGGTGCG TTCACTGCCG TATAGGCAGT CGCTGGGACG
     1081 CCCGGGATCC AAGAGATTTC TACACGATTG AGCACTGTCT CAGCCTGCGG TCCGGGTTCA
     1141 CTGCCGTATA GGCAGTAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGTAAAGG
     1201 CGAAGAACTG TTTACCGGTG TGGTTCCGAT TCTGGTGGAA CTGGACGGCG ATGTTAATGG
     1261 TCATAAATTC AGTGTTCGCG GCGAAGGTGA AGGCGATGCG ACGAACGGCA AACTGACCCT
     1321 GAAATTTATC TGCACCACGG GTAAACTGCC GGTCCCGTGG CCGACGCTGG TGACCACGCT
     1381 GACCTATGGC GTTCAATGTT TTGCGCGTTA CCCGGATCAC ATGAAACAGC ACGACTTTTT
     1441 CAAATCGGCC ATGCCGGAAG GCTATGTGCA GGAACGTACG ATTAGCTTTA AAGACGATGG
     1501 TACGTATAAA ACCCGCGCGG AAGTGAAATT CGAAGGCGAT ACCCTGGTTA ACCGTATCGA
     1561 ACTGAAAGGT ATCGATTTCA AAGAAGACGG CAATATTCTG GGTCATAAAC TGGAATATAA
     1621 CTTCAATTCC CACAACGTGT ACATCACCGC GGATAAACAG AAAAACGGCA TTAAAGCCAA
     1681 TTTCAAAATC CGCCATAATG TGGAAGATGG TAGCGTTCAG CTGGCCGACC ACTATCAGCA
     1741 AAACACGCCG ATTGGTGATG GCCCGGTCCT GCTGCCGGAC AATCACTACC TGAGTACCCA
     1801 GTCCGTGCTG TCAAAAGATC CGAACGAAAA ACGTGACCAC ATGGTCCTGC TGGAATTTGT
     1861 GACGGCTGCG GGTATCACCC ACGGCATGGA CGAACTGTAT AAAATGTCCC GCCGTAATAC
     1921 TGACGCCATC ACAATCCACA GCATCCTGGA TTGGATTGAA GACTAATAAT CGCTGGGACG
     1981 CCCGCCTGCA GGCTCGGTAC CAAATTCCAG AAAAGAGGCC TCCCGAAAGG GGGGCCTTTT
     2041 TTCGTTTTGG TCCTAATAGA TAAAGGATAG GTCTGGTAGT GTTGTTCGTT CTCGCAGGTA
     2101 AATCAATAAT ACTCAGCAGT TCCGTAGACT TTTCAGTGGG ACAGGGTAGC GATAACAGAT
     2161 AGATTGTAAT AAGACACAGT AGGTGCTCGT AGTTGCGTGA AGAGAACCGC TCAGGAAATC
     2221 CAGTCAGAAG TATTGGTAAT CGTTGAAAAC TCAGTCGACG CACTTACTGA AGACGTCCTA
     2281 TTACACTCGT CGTTGGAAAC TGAAGATGCG GCCGCggaaa cacagAAAAA AGCCCGCACC
     2341 TGACAGTGCG GGCTTTTTTT TTcgaccaaa ggTAGCGAAC GACGAGTCAC TGTTGAGGAT
     2401 AAATACTTTC TCTACTAGGC GCCTGTTACA CAGGTCCTCA GCGGCGCGCC TTTGTCGGTG
     2461 AACGCTCTCC TGAGTAGGAC AAATCCGCCG GGAGCGGATT TGAACGTTGT GAAGCAACGG
     2521 CCCGGAGGGT GGCGGGCAGG ACGCCCGCCA TAAACTGCCA GGCATCAAAC TAAGCAGAAG
     2581 GCCATCCTGA CGGATGGCCT TTTTGCGTTT CAGATCTACC GGTaaaccag caatagacat
     2641 aagcggctat ttaacgaccc tgccctgaac cgacgacaag ctgacgaccg ggtctccgca
     2701 agtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2761 tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa
     2821 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     2881 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     2941 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     3001 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     3061 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     3121 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcggtcgct
     3181 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     3241 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     3301 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     3361 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     3421 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     3481 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     3541 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     3601 gctcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3661 cggatacata tttgaatgta tttagaaaaa taaacaaata ggcatgctag cgcagaaacg
     3721 tcctagaaga tgccaggagg atacttagca gagagacaat aaggccggag cgaagccgtt
     3781 tttccatagg ctccgccccc ctgacgaaca tcacgaaatc tgacgctcaa atcagtggtg
     3841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctgatggct ccctcttgcg
     3901 ctctcctgtt cccgtcctgc ggcgtccgtg ttgtggtgga ggctttaccc aaatcaccac
     3961 gtcccgttcc gtgtagacag ttcgctccaa gctgggctgt gtgcaagaac cccccgttca
     4021 gcccgactgc tgcgccttat ccggtaacta tcatcttgag tccaacccgg aaagacacga
     4081 caaaacgcca ctggcagcag ccattggtaa ctgagaatta gtggatttag atatcgagag
     4141 tcttgaagtg gtggcctaac agaggctaca ctgaaaggac agtatttggt atctgcgctc
     4201 cactaaagcc agttaccagg ttaagcagtt ccccaactga cttaaccttc gatcaaaccg
     4261 cctccccagg cggttttttc gtttacagag caggagatta cgacgatcgt aaaaggatct
     4321 caagaagatc ctttacggat tcccgacacc atcactctag a
//