LOCUS pJ2076.2_cL 4415 bp ds-DNA circular 28-FEB-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature 2370..2426 /label="ECK120033737 Terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac gene 1244..1957 /label="sfGFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2362..2369 /label="NotI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 583..782 /label="Spacer 1" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 937..966 /label="Linker_19" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 191..220 /label="Linker_1" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature complement(899..921) /label="bs-1" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 37..42 /label="SacI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2427..2471 /label="Linker_8" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 487..492 /label="NcoI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 93..107 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 839..853 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 43..57 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 176..190 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2108..2307 /label="Spacer 2.5" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 493..582 /label="ECK120029600 Terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 2314..2325 /label="Spacer 5" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 884..898 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 804..838 /label="P(BBa_J23102)" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 oriT 3770..4405 /label="ColA ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 1210..1243 /label="RBS" /ApEinfo_revcolor=#7ea7d8 /ApEinfo_fwdcolor=#7ea7d8 misc_feature 2047..2107 /label="L3S2P21 Terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 967..981 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2326..2331 /label="AatII" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 108..137 /label="Linker_21" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1002..1103 /label="sgRNA-4 t4" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 451..456 /label="XhoI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2490..2496 /label="BbvCI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature complement(153..175) /label="bs-4" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2024..2038 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 982..1001 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1139..1144 /label="BamHI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 138..152 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1104..1123 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1124..1138 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1145..1174 /label="Linker_10" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 789..803 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 gene complement(2845..3660) /label="KanR" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 2308..2313 /label="SalI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 854..883 /label="Linker_24" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2472..2477 /label="KasI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 436..450 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2039..2046 /label="SbfI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 221..235 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 922..936 /label="Suffix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 7..36 /label="Linker_14" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 58..92 /label="P(BBa_J23100)" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 457..486 /label="Linker_0" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1190..1209 /label="Csy4 site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2478..2489 /label="Spacer 15" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2497..2504 /label="AscI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1..6 /label="EcoRI" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 236..435 /label="Spacer 2.2" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1175..1189 /label="Prefix" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2332..2361 /label="Linker_11" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1958..2023 /label="MarAn20" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 783..788 /label="HindIII" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd ORIGIN 1 GAATTCATAG GATAGATTCT GGAAACTTTA CCGTCCGAGC TCCAGCCTGC GGTCCGGttg 61 acggctagct cagtcctagg tacagtgcta gcTCGCTGGG ACGCCCGAGA GCCGAATCGC 121 ACTTATTTAC AGTAGTTCAG CCTGCGGTCC GGccaTACAT ACTCTAAGAT GTGTCTCGCT 181 GGGACGCCCG GGGACTACAC TTACGAAACT ATTGATTGCT CAGCCTGCGG TCCGGTGTAA 241 GAGAAATCCA CGAAGGAAAA GGTGAAAGCA GAACAGATAA TCAGTAATAG TAGCAACTCA 301 ACCACAGGAC ACTTCCCTCA CTCCGAACGA ATCACAATAC GCTCCAATCT TCAACTACAC 361 GATAAAACTG GTAATCCCAA CAACCTGACT CACACTCTAC TTTCTCAACG AGGAACTTCT 421 TATTCTGATA CAATCTCGCT GGGACGCCCG CTCGAGCAAT AAACAGTTGA TAGGGCTTCT 481 CCGTTACCAT GGTTCAGCCA AAAAACTTAA GACCGCCGGT CTTGTCCACT ACCTTGCAGT 541 AATGCGGTGG ACAGGATCGG CGGTTTTCTT TTCTCTTCTC AATTCTTCTG ACCTGTAACG 601 AATAATAGAT AGTAAAGTAG TCTCCGATTG AGTTTTCTCT GCCGAGTCCC ACCCAGTTCT 661 GTGATTTCAG TAAGTTGGTA ATTGATACAC TGTTGCGAGA ACTGCTGCCT GGTAGTAGAT 721 AGGTTGTTAT TGAGTAAGAA GGTAAAGTGA ACGAAATCCC TGAAACTGAG ACTGTAGAAA 781 ATAAGCTTCA GCCTGCGGTC CGGTTGACAG CTAGCTCAGT CCTAGGTACT GTGCTAGCTC 841 GCTGGGACGC CCGAGATAGC CGTTACACAG GTGACACTTA TTTCAGCCTG CGGTCCGGcc 901 aCACTAGAGT CTAGCTTGAG ATCGCTGGGA CGCCCGTGAA CAGTTGCTCT GATTGAAACC 961 ACGATTCAGC CTGCGGTCCG GGTTCACTGC CGTATAGGCA GCATCTTAGA GTATGTAGTT 1021 TcAGAGCTAT GCTGGAAACA GCATAGCAAG TTgAAATAAG GCTAGTCCGT TATCAACTTG 1081 AAAAAGTGGC ACCGAGTCGG TGCGTTCACT GCCGTATAGG CAGTCGCTGG GACGCCCGGG 1141 ATCCAAGAGA TTTCTACACG ATTGAGCACT GTCTCAGCCT GCGGTCCGGG TTCACTGCCG 1201 TATAGGCAGT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGCGTA AAGGCGAAGA 1261 ACTGTTTACC GGTGTGGTTC CGATTCTGGT GGAACTGGAC GGCGATGTTA ATGGTCATAA 1321 ATTCAGTGTT CGCGGCGAAG GTGAAGGCGA TGCGACGAAC GGCAAACTGA CCCTGAAATT 1381 TATCTGCACC ACGGGTAAAC TGCCGGTCCC GTGGCCGACG CTGGTGACCA CGCTGACCTA 1441 TGGCGTTCAA TGTTTTGCGC GTTACCCGGA TCACATGAAA CAGCACGACT TTTTCAAATC 1501 GGCCATGCCG GAAGGCTATG TGCAGGAACG TACGATTAGC TTTAAAGACG ATGGTACGTA 1561 TAAAACCCGC GCGGAAGTGA AATTCGAAGG CGATACCCTG GTTAACCGTA TCGAACTGAA 1621 AGGTATCGAT TTCAAAGAAG ACGGCAATAT TCTGGGTCAT AAACTGGAAT ATAACTTCAA 1681 TTCCCACAAC GTGTACATCA CCGCGGATAA ACAGAAAAAC GGCATTAAAG CCAATTTCAA 1741 AATCCGCCAT AATGTGGAAG ATGGTAGCGT TCAGCTGGCC GACCACTATC AGCAAAACAC 1801 GCCGATTGGT GATGGCCCGG TCCTGCTGCC GGACAATCAC TACCTGAGTA CCCAGTCCGT 1861 GCTGTCAAAA GATCCGAACG AAAAACGTGA CCACATGGTC CTGCTGGAAT TTGTGACGGC 1921 TGCGGGTATC ACCCACGGCA TGGACGAACT GTATAAAATG TCCCGCCGTA ATACTGACGC 1981 CATCACAATC CACAGCATCC TGGATTGGAT TGAAGACTAA TAATCGCTGG GACGCCCGCC 2041 TGCAGGCTCG GTACCAAATT CCAGAAAAGA GGCCTCCCGA AAGGGGGGCC TTTTTTCGTT 2101 TTGGTCCTAA TAGATAAAGG ATAGGTCTGG TAGTGTTGTT CGTTCTCGCA GGTAAATCAA 2161 TAATACTCAG CAGTTCCGTA GACTTTTCAG TGGGACAGGG TAGCGATAAC AGATAGATTG 2221 TAATAAGACA CAGTAGGTGC TCGTAGTTGC GTGAAGAGAA CCGCTCAGGA AATCCAGTCA 2281 GAAGTATTGG TAATCGTTGA AAACTCAGTC GACGCACTTA CTGAAGACGT CCTATTACAC 2341 TCGTCGTTGG AAACTGAAGA TGCGGCCGCg gaaacacagA AAAAAGCCCG CACCTGACAG 2401 TGCGGGCTTT TTTTTTcgac caaaggTAGC GAACGACGAG TCACTGTTGA GGATAAATAC 2461 TTTCTCTACT AGGCGCCTGT TACACAGGTC CTCAGCGGCG CGCCTTTGTC GGTGAACGCT 2521 CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGTGAAGCA ACGGCCCGGA 2581 GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC AAACTAAGCA GAAGGCCATC 2641 CTGACGGATG GCCTTTTTGC GTTTCAGATC TACCGGTaaa ccagcaatag acataagcgg 2701 ctatttaacg accctgccct gaaccgacga caagctgacg accgggtctc cgcaagtggc 2761 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 2821 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa 2881 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg 2941 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc 3001 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag 3061 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc 3121 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 3181 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcggt cgctgttaaa 3241 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 3301 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 3361 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 3421 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 3481 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 3541 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 3601 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat 3661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 3721 catatttgaa tgtatttaga aaaataaaca aataggcatg ctagcgcaga aacgtcctag 3781 aagatgccag gaggatactt agcagagaga caataaggcc ggagcgaagc cgtttttcca 3841 taggctccgc ccccctgacg aacatcacga aatctgacgc tcaaatcagt ggtggcgaaa 3901 cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctct tgcgctctcc 3961 tgttcccgtc ctgcggcgtc cgtgttgtgg tggaggcttt acccaaatca ccacgtcccg 4021 ttccgtgtag acagttcgct ccaagctggg ctgtgtgcaa gaaccccccg ttcagcccga 4081 ctgctgcgcc ttatccggta actatcatct tgagtccaac ccggaaagac acgacaaaac 4141 gccactggca gcagccattg gtaactgaga attagtggat ttagatatcg agagtcttga 4201 agtggtggcc taacagaggc tacactgaaa ggacagtatt tggtatctgc gctccactaa 4261 agccagttac caggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc 4321 caggcggttt tttcgtttac agagcaggag attacgacga tcgtaaaagg atctcaagaa 4381 gatcctttac ggattcccga caccatcact ctaga //