LOCUS       pJ2042.2_Bs             5864 bp ds-DNA     circular     28-FEB-2020
DEFINITION  .
KEYWORDS    "or pN1-011 + pN2-011 + pN3-011" "pN1-006.2 + pN2-007 + pN3-007"   
FEATURES             Location/Qualifiers
     misc_feature    321..350
                     /label="Linker_14"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    392..493
                     /label="sgRNA-1t4"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2135..2195
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2452..2466
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    372..391
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    735..740
                     /label="XhoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1168..1182
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    771..776
                     /label="NcoI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2902..2907
                     /label="KasI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1257..1271
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            complement(4986..5864)
                     /label="araC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1123..1137
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2512..2534)
                     /label="bs-2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    777..866
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2196..2395
                     /label="Spacer 2.5"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2550..2579
                     /label="Linker_17"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2396..2401
                     /label="SalI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2417..2451
                     /label="P(BBa_J23150)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2857..2901
                     /label="Linker_8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    357..371
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2762..2791
                     /label="Linker_11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2741..2755
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    741..770
                     /label="Linker_0"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     oriT            4200..4835
                     /label="ColA ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1206..1220
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1067..1072
                     /label="HindIII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    594..699
                     /label="sgRNA-2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    700..719
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1183..1205)
                     /label="bs-1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2497..2511
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    574..593
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2580..2594
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2908..2919
                     /label="Spacer 15"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2535..2549
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2721..2740
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1088..1122
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2127..2134
                     /label="SbfI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2402..2416
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    559..573
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2595..2614
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2792..2799
                     /label="NotI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    514..528
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2927..2934
                     /label="AscI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    529..558
                     /label="Linker_1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1138..1167
                     /label="Linker_24"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2467..2496
                     /label="Linker_25"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    494..513
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1272..1291
                     /label="Csy4 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        274..302
                     /label="P(BAD) promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1326..2045
                     /label="mKate2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1292..1325
                     /label="RBS"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1073..1087
                     /label="Prefix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2615..2720
                     /label="sgRNA-1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1221..1226
                     /label="BamHI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            complement(3275..4090)
                     /label="KanR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    315..320
                     /label="EcoRI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2920..2926
                     /label="BbvCI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2112..2126
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    351..356
                     /label="SacI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    867..1066
                     /label="Spacer 1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2800..2856
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2756..2761
                     /label="AatII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    720..734
                     /label="Suffix"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1227..1256
                     /label="Linker_10"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2046..2111
                     /label="MarAn20"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 ACTTTTCATA CTCCCGCCAT TCAGAGAAGA AACCAATTGT CCATATTGCA TCAGACATTG
       61 CCGTCACTGC GTCTTTTACT GGCTCTTCTC GCTAACCAAA CCGGTAACCC CGCTTATTAA
      121 AAGCATTCTG TAACAAAGCG GGACCAAAGC CATGACAAAA ACGCGTAACA AAAGTGTCTA
      181 TAATCACGGC AGAAAAGTCC ACATTGATTA TTTGCACGGC GTCACACTTT GCTATGCCAT
      241 AGCATTTTTA TCCATAAGAT TAGCGGtTCC TACCTGACGC TTTTTATCGC AACTCTCTAC
      301 TGTTTCTCCA TACCGAATTC ATAGGATAGA TTCTGGAAAC TTTACCGTCC GAGCTCCAGC
      361 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GAAGCTAGAC TCTAGTGGTT TcAGAGCTAT
      421 GCTGGAAACA GCATAGCAAG TTgAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
      481 ACCGAGTCGG TGCGTTCACT GCCGTATAGG CAGTCGCTGG GACGCCCGGG GACTACACTT
      541 ACGAAACTAT TGATTGCTCA GCCTGCGGTC CGGGTTCACT GCCGTATAGG CAGATCAGTG
      601 TGTACTAAGT ACTGTTTcAG AGCTATGCTG GAAACAGCAT AGCAAGTTgA AATAAGGCTA
      661 GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCG TTCACTGCCG TATAGGCAGT
      721 CGCTGGGACG CCCGCTCGAG CAATAAACAG TTGATAGGGC TTCTCCGTTA CCATGGTTCA
      781 GCCAAAAAAC TTAAGACCGC CGGTCTTGTC CACTACCTTG CAGTAATGCG GTGGACAGGA
      841 TCGGCGGTTT TCTTTTCTCT TCTCAATTCT TCTGACCTGT AACGAATAAT AGATAGTAAA
      901 GTAGTCTCCG ATTGAGTTTT CTCTGCCGAG TCCCACCCAG TTCTGTGATT TCAGTAAGTT
      961 GGTAATTGAT ACACTGTTGC GAGAACTGCT GCCTGGTAGT AGATAGGTTG TTATTGAGTA
     1021 AGAAGGTAAA GTGAACGAAA TCCCTGAAAC TGAGACTGTA GAAAATAAGC TTCAGCCTGC
     1081 GGTCCGGttg acggctagct cagtcctagg tacagtgcta gcTCGCTGGG ACGCCCGAGA
     1141 TAGCCGTTAC ACAGGTGACA CTTATTTCAG CCTGCGGTCC GGccaCACTA GAGTCTAGCT
     1201 TGAGATCGCT GGGACGCCCG GGATCCAAGA GATTTCTACA CGATTGAGCA CTGTCTCAGC
     1261 CTGCGGTCCG GGTTCACTGC CGTATAGGCA GTAATTTTGT TTAACTTTAA GAAGGAGATA
     1321 TACATatggt tagtaaagga gaagaaaata acatggcaCT GATTAAGGAG AACATGCACA
     1381 TGAAGCTGTA CATGGAGGGC ACCGTGAACA ACCACCACTT CAAGTGCACA TCCGAGGGCG
     1441 AAGGCAAGCC CTACGAGGGC ACCCAGACCA TGAGAATCAA GgccGTCGAG GGCGGCCCTC
     1501 TCCCCTTCGC CTTCGACATC CTGGCTACCA GCTTCATGTA CGGCAGCAAA ACCTTCATCA
     1561 ACCACACCCA GGGCATCCCC GACTTCTTTA AGCAGTCCTT CCCTGAGGGC TTCACATGGG
     1621 AGAGAGTCAC CACATACGAA GACGGGGGCG TGCTGACCGC TACCCAGGAC ACCAGCCTCC
     1681 AGGACGGCTG CCTCATCTAC AACGTCAAGA TCAGAGGGGT GAACTTCCCA TCCAACGGCC
     1741 CTGTGATGCA GAAGAAAACA CTCGGCTGGG AGGCCTCCAC CGAGaccCTG TACCCCGCTG
     1801 ACGGCGGCCT GGAAGGCAGA gcCGACATGG CCCTGAAGCT CGTGGGCGGG GGCCACCTGA
     1861 TCTGCAACTT GAAGACCACA TACAGATCCA AGAAACCCGC TAAGAACCTC AAGATGCCCG
     1921 GCGTCTACTA TGTGGACAGA AGACTGGAAA GAATCAAGGA GGCCGACAAA GAGACCTACG
     1981 TCGAGCAGCA CGAGGTGGCT GTGGCCAGAT ACTGCGACCT CCCTAGCAAA CTGGGGCACA
     2041 gAgtcATGTC CCGCCGTAAT ACTGACGCCA TCACAATCCA CAGCATCCTG GATTGGATTG
     2101 AAGACTAATA ATCGCTGGGA CGCCCGCCTG CAGGCTCGGT ACCAAATTCC AGAAAAGAGG
     2161 CCTCCCGAAA GGGGGGCCTT TTTTCGTTTT GGTCCTAATA GATAAAGGAT AGGTCTGGTA
     2221 GTGTTGTTCG TTCTCGCAGG TAAATCAATA ATACTCAGCA GTTCCGTAGA CTTTTCAGTG
     2281 GGACAGGGTA GCGATAACAG ATAGATTGTA ATAAGACACA GTAGGTGCTC GTAGTTGCGT
     2341 GAAGAGAACC GCTCAGGAAA TCCAGTCAGA AGTATTGGTA ATCGTTGAAA ACTCAGTCGA
     2401 CCAGCCTGCG GTCCGGTTTA CGGCTAGCTC AGTCCTAGGT ATTATGCTAG CTCGCTGGGA
     2461 CGCCCGAGTG ACGACTGCGA AGTAACCTCT ATTTATCAGC CTGCGGTCCG GccaAGTACT
     2521 TAGTACACAC TGATTCGCTG GGACGCCCGG TAGTGCTTAT CAGACCCAAT ACTGTTGAAC
     2581 AGCCTGCGGT CCGGGTTCAC TGCCGTATAG GCAGTCTCAA GCTAGACTCT AGTGGTTTcA
     2641 GAGCTATGCT GGAAACAGCA TAGCAAGTTg AAATAAGGCT AGTCCGTTAT CAACTTGAAA
     2701 AAGTGGCACC GAGTCGGTGC GTTCACTGCC GTATAGGCAG TCGCTGGGAC GCCCGGACGT
     2761 CCTATTACAC TCGTCGTTGG AAACTGAAGA TGCGGCCGCg gaaacacagA AAAAAGCCCG
     2821 CACCTGACAG TGCGGGCTTT TTTTTTcgac caaaggTAGC GAACGACGAG TCACTGTTGA
     2881 GGATAAATAC TTTCTCTACT AGGCGCCTGT TACACAGGTC CTCAGCGGCG CGCCTTTGTC
     2941 GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGTGAAGCA
     3001 ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC AAACTAAGCA
     3061 GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCAGATC TACCGGTaaa ccagcaatag
     3121 acataagcgg ctatttaacg accctgccct gaaccgacga caagctgacg accgggtctc
     3181 cgcaagtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3241 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat
     3301 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     3361 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     3421 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     3481 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     3541 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     3601 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcggt
     3661 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     3721 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     3781 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     3841 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     3901 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     3961 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     4021 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
     4081 tatggctcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggcatg ctagcgcaga
     4201 aacgtcctag aagatgccag gaggatactt agcagagaga caataaggcc ggagcgaagc
     4261 cgtttttcca taggctccgc ccccctgacg aacatcacga aatctgacgc tcaaatcagt
     4321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctct
     4381 tgcgctctcc tgttcccgtc ctgcggcgtc cgtgttgtgg tggaggcttt acccaaatca
     4441 ccacgtcccg ttccgtgtag acagttcgct ccaagctggg ctgtgtgcaa gaaccccccg
     4501 ttcagcccga ctgctgcgcc ttatccggta actatcatct tgagtccaac ccggaaagac
     4561 acgacaaaac gccactggca gcagccattg gtaactgaga attagtggat ttagatatcg
     4621 agagtcttga agtggtggcc taacagaggc tacactgaaa ggacagtatt tggtatctgc
     4681 gctccactaa agccagttac caggttaagc agttccccaa ctgacttaac cttcgatcaa
     4741 accgcctccc caggcggttt tttcgtttac agagcaggag attacgacga tcgtaaaagg
     4801 atctcaagaa gatcctttac ggattcccga caccatcact ctagatttca gtgcaattta
     4861 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt
     4921 agtcatgccc cgcgcccacc ggaaggagct gactgggttg CTCCTAgGGT CTGATTCGTT
     4981 ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC
     5041 TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA
     5101 AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC
     5161 GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG
     5221 AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA
     5281 AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC
     5341 ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC
     5401 AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC
     5461 CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG
     5521 GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC
     5581 CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC
     5641 GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC
     5701 CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA
     5761 AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA
     5821 AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCAT
//